Amino acid dipepetide frequency for Elusimicrobium sp. An273

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.833AlaAla: 12.833 ± 0.298
1.485AlaCys: 1.485 ± 0.063
5.195AlaAsp: 5.195 ± 0.117
5.9AlaGlu: 5.9 ± 0.138
4.023AlaPhe: 4.023 ± 0.094
7.294AlaGly: 7.294 ± 0.138
1.57AlaHis: 1.57 ± 0.061
4.147AlaIle: 4.147 ± 0.111
6.268AlaLys: 6.268 ± 0.152
10.716AlaLeu: 10.716 ± 0.176
2.069AlaMet: 2.069 ± 0.065
2.984AlaAsn: 2.984 ± 0.099
4.21AlaPro: 4.21 ± 0.159
5.585AlaGln: 5.585 ± 0.133
5.406AlaArg: 5.406 ± 0.135
5.262AlaSer: 5.262 ± 0.129
3.45AlaThr: 3.45 ± 0.101
7.952AlaVal: 7.952 ± 0.17
1.102AlaTrp: 1.102 ± 0.051
2.986AlaTyr: 2.986 ± 0.082
0.0AlaXaa: 0.0 ± 0.0
Cys
1.368CysAla: 1.368 ± 0.064
0.213CysCys: 0.213 ± 0.025
0.512CysAsp: 0.512 ± 0.043
0.629CysGlu: 0.629 ± 0.039
0.523CysPhe: 0.523 ± 0.037
1.298CysGly: 1.298 ± 0.058
0.222CysHis: 0.222 ± 0.022
0.595CysIle: 0.595 ± 0.037
0.614CysLys: 0.614 ± 0.039
1.098CysLeu: 1.098 ± 0.056
0.25CysMet: 0.25 ± 0.024
0.447CysAsn: 0.447 ± 0.033
0.701CysPro: 0.701 ± 0.046
0.423CysGln: 0.423 ± 0.031
0.656CysArg: 0.656 ± 0.038
0.767CysSer: 0.767 ± 0.046
0.677CysThr: 0.677 ± 0.043
0.956CysVal: 0.956 ± 0.045
0.139CysTrp: 0.139 ± 0.017
0.423CysTyr: 0.423 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
4.273AspAla: 4.273 ± 0.105
0.555AspCys: 0.555 ± 0.035
1.997AspAsp: 1.997 ± 0.064
3.073AspGlu: 3.073 ± 0.087
2.736AspPhe: 2.736 ± 0.072
3.496AspGly: 3.496 ± 0.104
0.695AspHis: 0.695 ± 0.039
3.343AspIle: 3.343 ± 0.084
3.28AspLys: 3.28 ± 0.091
5.088AspLeu: 5.088 ± 0.115
1.161AspMet: 1.161 ± 0.059
1.784AspAsn: 1.784 ± 0.072
2.409AspPro: 2.409 ± 0.072
1.294AspGln: 1.294 ± 0.054
2.137AspArg: 2.137 ± 0.071
2.313AspSer: 2.313 ± 0.075
2.35AspThr: 2.35 ± 0.078
3.657AspVal: 3.657 ± 0.088
0.693AspTrp: 0.693 ± 0.037
2.06AspTyr: 2.06 ± 0.081
0.0AspXaa: 0.0 ± 0.0
Glu
5.534GluAla: 5.534 ± 0.131
0.499GluCys: 0.499 ± 0.032
2.969GluAsp: 2.969 ± 0.075
4.809GluGlu: 4.809 ± 0.13
2.276GluPhe: 2.276 ± 0.069
3.757GluGly: 3.757 ± 0.097
1.157GluHis: 1.157 ± 0.054
4.121GluIle: 4.121 ± 0.091
5.591GluLys: 5.591 ± 0.134
5.759GluLeu: 5.759 ± 0.119
1.712GluMet: 1.712 ± 0.059
2.967GluAsn: 2.967 ± 0.081
2.1GluPro: 2.1 ± 0.077
2.725GluGln: 2.725 ± 0.082
3.006GluArg: 3.006 ± 0.092
2.402GluSer: 2.402 ± 0.067
3.258GluThr: 3.258 ± 0.091
3.936GluVal: 3.936 ± 0.094
0.512GluTrp: 0.512 ± 0.032
1.932GluTyr: 1.932 ± 0.083
0.0GluXaa: 0.0 ± 0.0
Phe
4.219PheAla: 4.219 ± 0.107
0.706PheCys: 0.706 ± 0.04
2.518PheAsp: 2.518 ± 0.077
2.444PheGlu: 2.444 ± 0.081
2.511PhePhe: 2.511 ± 0.095
3.25PheGly: 3.25 ± 0.084
0.719PheHis: 0.719 ± 0.042
2.596PheIle: 2.596 ± 0.091
2.751PheLys: 2.751 ± 0.083
4.837PheLeu: 4.837 ± 0.13
1.176PheMet: 1.176 ± 0.052
1.904PheAsn: 1.904 ± 0.065
1.662PhePro: 1.662 ± 0.066
1.167PheGln: 1.167 ± 0.053
1.681PheArg: 1.681 ± 0.061
3.187PheSer: 3.187 ± 0.087
2.818PheThr: 2.818 ± 0.084
3.021PheVal: 3.021 ± 0.087
0.549PheTrp: 0.549 ± 0.037
1.721PheTyr: 1.721 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
6.264GlyAla: 6.264 ± 0.14
0.926GlyCys: 0.926 ± 0.046
2.873GlyAsp: 2.873 ± 0.088
3.964GlyGlu: 3.964 ± 0.095
3.441GlyPhe: 3.441 ± 0.094
5.206GlyGly: 5.206 ± 0.15
1.346GlyHis: 1.346 ± 0.061
4.493GlyIle: 4.493 ± 0.109
5.412GlyLys: 5.412 ± 0.12
6.73GlyLeu: 6.73 ± 0.131
1.76GlyMet: 1.76 ± 0.069
2.516GlyAsn: 2.516 ± 0.081
2.06GlyPro: 2.06 ± 0.076
2.49GlyGln: 2.49 ± 0.07
3.4GlyArg: 3.4 ± 0.09
3.596GlySer: 3.596 ± 0.102
4.01GlyThr: 4.01 ± 0.097
5.243GlyVal: 5.243 ± 0.108
0.926GlyTrp: 0.926 ± 0.045
2.474GlyTyr: 2.474 ± 0.084
0.0GlyXaa: 0.0 ± 0.0
His
1.507HisAla: 1.507 ± 0.058
0.274HisCys: 0.274 ± 0.025
0.701HisAsp: 0.701 ± 0.044
0.812HisGlu: 0.812 ± 0.04
0.915HisPhe: 0.915 ± 0.048
1.298HisGly: 1.298 ± 0.063
0.357HisHis: 0.357 ± 0.027
1.237HisIle: 1.237 ± 0.046
1.019HisLys: 1.019 ± 0.044
1.827HisLeu: 1.827 ± 0.061
0.464HisMet: 0.464 ± 0.031
0.656HisAsn: 0.656 ± 0.039
1.187HisPro: 1.187 ± 0.052
0.577HisGln: 0.577 ± 0.034
0.775HisArg: 0.775 ± 0.037
0.923HisSer: 0.923 ± 0.046
1.137HisThr: 1.137 ± 0.044
1.109HisVal: 1.109 ± 0.053
0.176HisTrp: 0.176 ± 0.019
0.741HisTyr: 0.741 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
5.306IleAla: 5.306 ± 0.123
0.712IleCys: 0.712 ± 0.048
2.779IleAsp: 2.779 ± 0.08
3.274IleGlu: 3.274 ± 0.085
2.614IlePhe: 2.614 ± 0.092
3.892IleGly: 3.892 ± 0.108
0.943IleHis: 0.943 ± 0.048
3.35IleIle: 3.35 ± 0.111
3.518IleLys: 3.518 ± 0.109
5.739IleLeu: 5.739 ± 0.104
1.139IleMet: 1.139 ± 0.05
2.224IleAsn: 2.224 ± 0.079
2.655IlePro: 2.655 ± 0.086
1.616IleGln: 1.616 ± 0.063
2.607IleArg: 2.607 ± 0.077
3.724IleSer: 3.724 ± 0.1
3.378IleThr: 3.378 ± 0.094
3.792IleVal: 3.792 ± 0.107
0.477IleTrp: 0.477 ± 0.036
1.962IleTyr: 1.962 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
5.837LysAla: 5.837 ± 0.154
0.538LysCys: 0.538 ± 0.043
3.727LysAsp: 3.727 ± 0.113
5.223LysGlu: 5.223 ± 0.132
2.102LysPhe: 2.102 ± 0.071
4.008LysGly: 4.008 ± 0.112
1.311LysHis: 1.311 ± 0.055
4.195LysIle: 4.195 ± 0.1
6.025LysLys: 6.025 ± 0.167
5.713LysLeu: 5.713 ± 0.114
2.411LysMet: 2.411 ± 0.068
3.692LysAsn: 3.692 ± 0.099
2.775LysPro: 2.775 ± 0.085
2.892LysGln: 2.892 ± 0.088
3.197LysArg: 3.197 ± 0.084
3.093LysSer: 3.093 ± 0.073
4.681LysThr: 4.681 ± 0.106
3.711LysVal: 3.711 ± 0.11
0.708LysTrp: 0.708 ± 0.041
2.198LysTyr: 2.198 ± 0.071
0.0LysXaa: 0.0 ± 0.0
Leu
10.627LeuAla: 10.627 ± 0.173
1.47LeuCys: 1.47 ± 0.059
4.909LeuAsp: 4.909 ± 0.116
6.022LeuGlu: 6.022 ± 0.122
4.652LeuPhe: 4.652 ± 0.129
6.722LeuGly: 6.722 ± 0.142
1.803LeuHis: 1.803 ± 0.066
4.988LeuIle: 4.988 ± 0.123
6.752LeuLys: 6.752 ± 0.131
10.093LeuLeu: 10.093 ± 0.214
2.298LeuMet: 2.298 ± 0.074
3.738LeuAsn: 3.738 ± 0.078
5.358LeuPro: 5.358 ± 0.114
4.112LeuGln: 4.112 ± 0.105
4.914LeuArg: 4.914 ± 0.115
6.693LeuSer: 6.693 ± 0.126
5.691LeuThr: 5.691 ± 0.116
5.881LeuVal: 5.881 ± 0.12
1.05LeuTrp: 1.05 ± 0.056
2.982LeuTyr: 2.982 ± 0.076
0.0LeuXaa: 0.0 ± 0.0
Met
2.3MetAla: 2.3 ± 0.08
0.237MetCys: 0.237 ± 0.023
1.396MetAsp: 1.396 ± 0.048
1.403MetGlu: 1.403 ± 0.056
0.847MetPhe: 0.847 ± 0.045
1.701MetGly: 1.701 ± 0.071
0.447MetHis: 0.447 ± 0.031
1.246MetIle: 1.246 ± 0.055
2.034MetLys: 2.034 ± 0.071
2.346MetLeu: 2.346 ± 0.081
0.579MetMet: 0.579 ± 0.041
1.115MetAsn: 1.115 ± 0.05
1.411MetPro: 1.411 ± 0.055
1.013MetGln: 1.013 ± 0.055
1.15MetArg: 1.15 ± 0.045
1.353MetSer: 1.353 ± 0.053
1.318MetThr: 1.318 ± 0.049
1.37MetVal: 1.37 ± 0.056
0.246MetTrp: 0.246 ± 0.022
0.634MetTyr: 0.634 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.781AsnAla: 3.781 ± 0.088
0.466AsnCys: 0.466 ± 0.034
1.597AsnAsp: 1.597 ± 0.058
1.999AsnGlu: 1.999 ± 0.075
1.83AsnPhe: 1.83 ± 0.067
2.903AsnGly: 2.903 ± 0.09
0.597AsnHis: 0.597 ± 0.041
2.518AsnIle: 2.518 ± 0.074
2.609AsnLys: 2.609 ± 0.084
3.812AsnLeu: 3.812 ± 0.106
1.013AsnMet: 1.013 ± 0.05
1.488AsnAsn: 1.488 ± 0.07
2.551AsnPro: 2.551 ± 0.084
1.294AsnGln: 1.294 ± 0.064
1.897AsnArg: 1.897 ± 0.068
1.832AsnSer: 1.832 ± 0.064
2.291AsnThr: 2.291 ± 0.086
2.494AsnVal: 2.494 ± 0.08
0.507AsnTrp: 0.507 ± 0.034
1.411AsnTyr: 1.411 ± 0.061
0.0AsnXaa: 0.0 ± 0.0
Pro
5.648ProAla: 5.648 ± 0.173
0.488ProCys: 0.488 ± 0.034
2.453ProAsp: 2.453 ± 0.067
3.156ProGlu: 3.156 ± 0.086
2.056ProPhe: 2.056 ± 0.076
2.943ProGly: 2.943 ± 0.077
0.969ProHis: 0.969 ± 0.046
1.984ProIle: 1.984 ± 0.071
2.507ProLys: 2.507 ± 0.078
4.164ProLeu: 4.164 ± 0.098
0.891ProMet: 0.891 ± 0.045
1.575ProAsn: 1.575 ± 0.056
1.494ProPro: 1.494 ± 0.06
2.733ProGln: 2.733 ± 0.09
1.788ProArg: 1.788 ± 0.07
2.631ProSer: 2.631 ± 0.078
2.167ProThr: 2.167 ± 0.065
3.748ProVal: 3.748 ± 0.101
0.427ProTrp: 0.427 ± 0.034
1.62ProTyr: 1.62 ± 0.063
0.0ProXaa: 0.0 ± 0.0
Gln
4.981GlnAla: 4.981 ± 0.121
0.311GlnCys: 0.311 ± 0.03
2.076GlnAsp: 2.076 ± 0.071
3.032GlnGlu: 3.032 ± 0.095
1.407GlnPhe: 1.407 ± 0.06
2.387GlnGly: 2.387 ± 0.07
0.708GlnHis: 0.708 ± 0.036
2.409GlnIle: 2.409 ± 0.072
3.208GlnLys: 3.208 ± 0.1
3.572GlnLeu: 3.572 ± 0.079
1.087GlnMet: 1.087 ± 0.051
1.899GlnAsn: 1.899 ± 0.062
1.651GlnPro: 1.651 ± 0.07
2.017GlnGln: 2.017 ± 0.087
1.74GlnArg: 1.74 ± 0.058
1.76GlnSer: 1.76 ± 0.057
2.781GlnThr: 2.781 ± 0.099
2.383GlnVal: 2.383 ± 0.078
0.359GlnTrp: 0.359 ± 0.028
1.313GlnTyr: 1.313 ± 0.059
0.0GlnXaa: 0.0 ± 0.0
Arg
4.694ArgAla: 4.694 ± 0.112
0.436ArgCys: 0.436 ± 0.031
2.013ArgAsp: 2.013 ± 0.077
3.149ArgGlu: 3.149 ± 0.088
2.407ArgPhe: 2.407 ± 0.086
2.919ArgGly: 2.919 ± 0.082
0.897ArgHis: 0.897 ± 0.041
2.742ArgIle: 2.742 ± 0.083
3.163ArgLys: 3.163 ± 0.086
5.023ArgLeu: 5.023 ± 0.114
1.311ArgMet: 1.311 ± 0.057
1.712ArgAsn: 1.712 ± 0.065
2.156ArgPro: 2.156 ± 0.079
2.47ArgGln: 2.47 ± 0.082
2.964ArgArg: 2.964 ± 0.091
2.254ArgSer: 2.254 ± 0.07
2.503ArgThr: 2.503 ± 0.076
3.243ArgVal: 3.243 ± 0.084
0.606ArgTrp: 0.606 ± 0.037
1.729ArgTyr: 1.729 ± 0.067
0.0ArgXaa: 0.0 ± 0.0
Ser
5.707SerAla: 5.707 ± 0.134
0.765SerCys: 0.765 ± 0.047
2.561SerAsp: 2.561 ± 0.077
2.679SerGlu: 2.679 ± 0.076
3.008SerPhe: 3.008 ± 0.081
4.393SerGly: 4.393 ± 0.114
0.941SerHis: 0.941 ± 0.046
2.67SerIle: 2.67 ± 0.077
3.267SerLys: 3.267 ± 0.086
5.744SerLeu: 5.744 ± 0.124
1.165SerMet: 1.165 ± 0.051
1.98SerAsn: 1.98 ± 0.075
2.344SerPro: 2.344 ± 0.076
1.936SerGln: 1.936 ± 0.07
2.775SerArg: 2.775 ± 0.09
3.788SerSer: 3.788 ± 0.115
2.544SerThr: 2.544 ± 0.083
4.408SerVal: 4.408 ± 0.107
0.66SerTrp: 0.66 ± 0.041
1.923SerTyr: 1.923 ± 0.069
0.0SerXaa: 0.0 ± 0.0
Thr
5.733ThrAla: 5.733 ± 0.138
0.608ThrCys: 0.608 ± 0.037
2.969ThrAsp: 2.969 ± 0.1
2.927ThrGlu: 2.927 ± 0.08
2.37ThrPhe: 2.37 ± 0.082
4.315ThrGly: 4.315 ± 0.085
0.947ThrHis: 0.947 ± 0.048
2.651ThrIle: 2.651 ± 0.079
2.749ThrLys: 2.749 ± 0.091
6.044ThrLeu: 6.044 ± 0.132
0.987ThrMet: 0.987 ± 0.049
1.664ThrAsn: 1.664 ± 0.067
3.535ThrPro: 3.535 ± 0.101
2.163ThrGln: 2.163 ± 0.07
2.461ThrArg: 2.461 ± 0.072
2.71ThrSer: 2.71 ± 0.078
2.513ThrThr: 2.513 ± 0.089
4.467ThrVal: 4.467 ± 0.115
0.462ThrTrp: 0.462 ± 0.039
1.625ThrTyr: 1.625 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
5.691ValAla: 5.691 ± 0.129
1.231ValCys: 1.231 ± 0.059
2.956ValAsp: 2.956 ± 0.088
3.925ValGlu: 3.925 ± 0.103
3.544ValPhe: 3.544 ± 0.095
4.062ValGly: 4.062 ± 0.105
1.189ValHis: 1.189 ± 0.05
3.99ValIle: 3.99 ± 0.096
4.546ValLys: 4.546 ± 0.104
7.824ValLeu: 7.824 ± 0.156
1.546ValMet: 1.546 ± 0.064
2.566ValAsn: 2.566 ± 0.081
3.143ValPro: 3.143 ± 0.093
2.659ValGln: 2.659 ± 0.086
3.552ValArg: 3.552 ± 0.099
4.729ValSer: 4.729 ± 0.107
3.783ValThr: 3.783 ± 0.102
4.835ValVal: 4.835 ± 0.124
0.745ValTrp: 0.745 ± 0.041
2.431ValTyr: 2.431 ± 0.082
0.0ValXaa: 0.0 ± 0.0
Trp
1.043TrpAla: 1.043 ± 0.052
0.118TrpCys: 0.118 ± 0.015
0.49TrpAsp: 0.49 ± 0.035
0.56TrpGlu: 0.56 ± 0.032
0.492TrpPhe: 0.492 ± 0.034
0.871TrpGly: 0.871 ± 0.05
0.194TrpHis: 0.194 ± 0.022
0.621TrpIle: 0.621 ± 0.037
0.686TrpLys: 0.686 ± 0.042
1.324TrpLeu: 1.324 ± 0.059
0.335TrpMet: 0.335 ± 0.03
0.512TrpAsn: 0.512 ± 0.038
0.409TrpPro: 0.409 ± 0.027
0.49TrpGln: 0.49 ± 0.033
0.54TrpArg: 0.54 ± 0.038
0.49TrpSer: 0.49 ± 0.035
0.518TrpThr: 0.518 ± 0.039
0.699TrpVal: 0.699 ± 0.038
0.172TrpTrp: 0.172 ± 0.019
0.362TrpTyr: 0.362 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.093TyrAla: 3.093 ± 0.092
0.473TyrCys: 0.473 ± 0.033
1.705TyrAsp: 1.705 ± 0.064
2.036TyrGlu: 2.036 ± 0.068
1.684TyrPhe: 1.684 ± 0.073
2.415TyrGly: 2.415 ± 0.079
0.682TyrHis: 0.682 ± 0.039
1.808TyrIle: 1.808 ± 0.069
2.036TyrLys: 2.036 ± 0.071
3.302TyrLeu: 3.302 ± 0.098
0.791TyrMet: 0.791 ± 0.039
1.47TyrAsn: 1.47 ± 0.063
1.631TyrPro: 1.631 ± 0.06
1.427TyrGln: 1.427 ± 0.062
1.742TyrArg: 1.742 ± 0.065
1.751TyrSer: 1.751 ± 0.064
2.028TyrThr: 2.028 ± 0.072
2.041TyrVal: 2.041 ± 0.07
0.412TyrTrp: 0.412 ± 0.031
1.372TyrTyr: 1.372 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1395 proteins (459124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski