Corynebacterium sp. 13CS0277
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S9Z2I3|A0A2S9Z2I3_9CORY Uncharacterized protein OS=Corynebacterium sp. 13CS0277 OX=2071994 GN=C1Y63_05065 PE=4 SV=1
MM1 pKa = 7.56 SEE3 pKa = 3.6 ATADD7 pKa = 3.4 IDD9 pKa = 4.3 GVDD12 pKa = 4.46 YY13 pKa = 10.78 PFDD16 pKa = 3.72 WPEE19 pKa = 4.0 DD20 pKa = 3.9 TTLLAAMLDD29 pKa = 3.63 AGIPAPHH36 pKa = 6.81 SCTLGQCGACRR47 pKa = 11.84 CTVEE51 pKa = 5.1 GPEE54 pKa = 3.64 STMRR58 pKa = 11.84 INQVLDD64 pKa = 3.9 DD65 pKa = 4.13 FDD67 pKa = 4.16 VRR69 pKa = 11.84 AGDD72 pKa = 3.74 RR73 pKa = 11.84 LACQTVRR80 pKa = 11.84 VPGDD84 pKa = 3.35 TTPAEE89 pKa = 3.59 ISYY92 pKa = 9.65 PFF94 pKa = 4.2
Molecular weight: 10.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 0.846
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A2S9Z187|A0A2S9Z187_9CORY DUF3566 domain-containing protein OS=Corynebacterium sp. 13CS0277 OX=2071994 GN=C1Y63_08250 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 6.02 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.71 GRR42 pKa = 11.84 ASLTAA47 pKa = 4.1
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2382
0
2382
817481
24
4452
343.2
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.606 ± 0.088
0.855 ± 0.017
6.011 ± 0.044
5.677 ± 0.055
3.048 ± 0.03
8.551 ± 0.061
2.338 ± 0.028
4.333 ± 0.038
2.6 ± 0.045
9.481 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.142 ± 0.022
2.283 ± 0.036
5.695 ± 0.049
3.274 ± 0.029
6.459 ± 0.054
5.078 ± 0.033
6.555 ± 0.055
8.487 ± 0.051
1.405 ± 0.023
2.122 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here