Streptococcus satellite phage Javan156
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFW3|A0A4D5ZFW3_9VIRU RelE/StbE replicon stabilization toxin OS=Streptococcus satellite phage Javan156 OX=2558547 GN=JavanS156_0025 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 TFSDD6 pKa = 3.67 TPKK9 pKa = 10.21 TFTFHH14 pKa = 5.7 YY15 pKa = 8.55 TFKK18 pKa = 11.1 DD19 pKa = 3.09 FDD21 pKa = 4.09 TAQVACHH28 pKa = 6.88 AILGYY33 pKa = 7.77 MTGTYY38 pKa = 7.21 EE39 pKa = 4.45 QPVVDD44 pKa = 4.0 ATYY47 pKa = 10.97 HH48 pKa = 6.27 NDD50 pKa = 3.55 DD51 pKa = 3.72 QGGHH55 pKa = 6.64 ANQLLLEE62 pKa = 4.17 YY63 pKa = 11.19 AEE65 pKa = 4.28 DD66 pKa = 3.54 RR67 pKa = 11.84 KK68 pKa = 10.83 LNKK71 pKa = 9.29 VFKK74 pKa = 10.18 RR75 pKa = 11.84 ICDD78 pKa = 3.69 SFKK81 pKa = 10.93 DD82 pKa = 4.21 YY83 pKa = 11.52 YY84 pKa = 10.51 NQPEE88 pKa = 4.58 DD89 pKa = 3.61 MTDD92 pKa = 4.14 DD93 pKa = 4.37 EE94 pKa = 6.25 LDD96 pKa = 4.42 DD97 pKa = 3.69 MAQEE101 pKa = 4.07 NEE103 pKa = 4.34 LIKK106 pKa = 10.6 EE107 pKa = 4.26 VEE109 pKa = 4.22 QPDD112 pKa = 3.96 GSII115 pKa = 3.69
Molecular weight: 13.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.178
IPC2_protein 4.266
IPC_protein 4.228
Toseland 4.024
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.126
Rodwell 4.05
Grimsley 3.935
Solomon 4.202
Lehninger 4.151
Nozaki 4.317
DTASelect 4.546
Thurlkill 4.062
EMBOSS 4.139
Sillero 4.342
Patrickios 3.147
IPC_peptide 4.202
IPC2_peptide 4.329
IPC2.peptide.svr19 4.259
Protein with the highest isoelectric point:
>tr|A0A4D5ZEM3|A0A4D5ZEM3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan156 OX=2558547 GN=JavanS156_0011 PE=4 SV=1
MM1 pKa = 7.1 YY2 pKa = 7.81 RR3 pKa = 11.84 TSVYY7 pKa = 10.65 LGVDD11 pKa = 2.96 QVTGKK16 pKa = 10.41 KK17 pKa = 10.32 ARR19 pKa = 11.84 TTITASTKK27 pKa = 10.32 KK28 pKa = 10.14 GVKK31 pKa = 9.43 IKK33 pKa = 10.92 ARR35 pKa = 11.84 DD36 pKa = 3.59 ALNNFAMNGYY46 pKa = 6.25 TVKK49 pKa = 10.52 EE50 pKa = 4.27 KK51 pKa = 9.63 PTVTTYY57 pKa = 11.25 KK58 pKa = 10.56 EE59 pKa = 4.08 LTALWWEE66 pKa = 4.56 SYY68 pKa = 11.25 KK69 pKa = 10.03 NTIKK73 pKa = 10.76 PNSRR77 pKa = 11.84 QSMEE81 pKa = 4.4 GIVRR85 pKa = 11.84 LHH87 pKa = 6.69 ILPAFGDD94 pKa = 4.16 CKK96 pKa = 10.5 LSRR99 pKa = 11.84 LTTPVIQQQVNKK111 pKa = 8.75 WANNANKK118 pKa = 10.06 GIKK121 pKa = 9.07 GAYY124 pKa = 9.42 ANYY127 pKa = 10.79 SFLNNINRR135 pKa = 11.84 RR136 pKa = 11.84 ILQYY140 pKa = 10.72 GVTMQVIEE148 pKa = 4.63 HH149 pKa = 6.18 NPARR153 pKa = 11.84 DD154 pKa = 3.48 VIIPRR159 pKa = 11.84 KK160 pKa = 9.5 QNNKK164 pKa = 5.23 EE165 pKa = 3.95 HH166 pKa = 6.3 KK167 pKa = 10.35 VKK169 pKa = 10.58 FFSNQEE175 pKa = 3.7 LKK177 pKa = 10.88 QFLNYY182 pKa = 10.28 LDD184 pKa = 5.7 DD185 pKa = 5.87 LDD187 pKa = 4.43 LSSYY191 pKa = 11.49 EE192 pKa = 3.95 NFFDD196 pKa = 3.77 YY197 pKa = 11.08 VLYY200 pKa = 8.87 KK201 pKa = 10.31 TLLATGCRR209 pKa = 11.84 IGEE212 pKa = 4.15 VLALEE217 pKa = 4.36 WSDD220 pKa = 3.01 IDD222 pKa = 5.15 LEE224 pKa = 4.49 KK225 pKa = 10.79 GTIKK229 pKa = 10.46 VSKK232 pKa = 8.99 TLNRR236 pKa = 11.84 YY237 pKa = 8.7 QEE239 pKa = 4.35 TNTPKK244 pKa = 10.61 SKK246 pKa = 10.87 AGLRR250 pKa = 11.84 DD251 pKa = 3.37 IEE253 pKa = 4.11 IDD255 pKa = 3.31 RR256 pKa = 11.84 ATVLLLKK263 pKa = 10.19 QYY265 pKa = 11.23 KK266 pKa = 9.66 NRR268 pKa = 11.84 QQVLSWDD275 pKa = 3.8 LGRR278 pKa = 11.84 SEE280 pKa = 5.22 TIVFTPFTTKK290 pKa = 10.34 YY291 pKa = 10.75 AYY293 pKa = 10.29 ACLLRR298 pKa = 11.84 KK299 pKa = 9.64 RR300 pKa = 11.84 LQKK303 pKa = 10.24 HH304 pKa = 5.51 FKK306 pKa = 10.01 AAGVPDD312 pKa = 3.77 ISFHH316 pKa = 5.86 GFRR319 pKa = 11.84 HH320 pKa = 4.4 THH322 pKa = 4.65 ATIMLYY328 pKa = 10.62 AGIEE332 pKa = 4.18 AKK334 pKa = 10.42 DD335 pKa = 3.35 LQYY338 pKa = 11.55 RR339 pKa = 11.84 LGHH342 pKa = 6.02 SNISMTLNTYY352 pKa = 8.7 VHH354 pKa = 6.14 ATKK357 pKa = 10.47 EE358 pKa = 4.25 GAKK361 pKa = 9.7 KK362 pKa = 10.18 AVSIFEE368 pKa = 4.16 TAISNLL374 pKa = 3.59
Molecular weight: 42.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.097
IPC2_protein 9.253
IPC_protein 9.151
Toseland 9.882
ProMoST 9.575
Dawson 10.101
Bjellqvist 9.765
Wikipedia 10.262
Rodwell 10.555
Grimsley 10.175
Solomon 10.116
Lehninger 10.072
Nozaki 9.882
DTASelect 9.765
Thurlkill 9.94
EMBOSS 10.292
Sillero 10.014
Patrickios 9.765
IPC_peptide 10.116
IPC2_peptide 8.302
IPC2.peptide.svr19 8.209
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25
0
25
3337
46
537
133.5
15.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.694 ± 0.491
0.599 ± 0.148
6.173 ± 0.527
8.421 ± 0.777
4.135 ± 0.37
4.405 ± 0.527
1.798 ± 0.213
6.263 ± 0.389
9.14 ± 0.397
9.739 ± 0.465
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.577 ± 0.24
5.454 ± 0.433
2.427 ± 0.175
4.555 ± 0.334
5.004 ± 0.422
5.514 ± 0.284
6.143 ± 0.653
6.023 ± 0.322
1.019 ± 0.146
4.915 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here