Sulfurihydrogenibium azorense (strain Az-Fu1 / DSM 15241 / OCM 825)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1708 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1DVU1|C1DVU1_SULAA Flagellar hook-associated protein 1 OS=Sulfurihydrogenibium azorense (strain Az-Fu1 / DSM 15241 / OCM 825) OX=204536 GN=flgK PE=3 SV=1
MM1 pKa = 7.14 KK2 pKa = 10.58 VKK4 pKa = 10.76 VSVDD8 pKa = 3.11 KK9 pKa = 10.97 DD10 pKa = 3.51 LCTACALCYY19 pKa = 10.74 DD20 pKa = 4.73 EE21 pKa = 5.71 VPEE24 pKa = 4.27 VYY26 pKa = 9.85 EE27 pKa = 4.96 DD28 pKa = 3.7 SGDD31 pKa = 4.09 GIAQVKK37 pKa = 10.6 AEE39 pKa = 4.08 VGGDD43 pKa = 3.46 GAIIEE48 pKa = 4.64 GDD50 pKa = 3.17 LAQRR54 pKa = 11.84 VLEE57 pKa = 4.21 VTEE60 pKa = 4.38 EE61 pKa = 4.36 CPSGALVTEE70 pKa = 4.84 VIEE73 pKa = 4.3 EE74 pKa = 4.11
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.872
IPC_protein 3.77
Toseland 3.592
ProMoST 3.872
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.643
Rodwell 3.605
Grimsley 3.503
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 3.999
Thurlkill 3.643
EMBOSS 3.656
Sillero 3.884
Patrickios 1.85
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>sp|C1DTV7|RSMH_SULAA Ribosomal RNA small subunit methyltransferase H OS=Sulfurihydrogenibium azorense (strain Az-Fu1 / DSM 15241 / OCM 825) OX=204536 GN=rsmH PE=3 SV=1
MM1 pKa = 7.71 ANMKK5 pKa = 10.3 AKK7 pKa = 10.35 SHH9 pKa = 6.63 LSNKK13 pKa = 8.22 KK14 pKa = 8.7 RR15 pKa = 11.84 KK16 pKa = 9.05 RR17 pKa = 11.84 VSGFLARR24 pKa = 11.84 MRR26 pKa = 11.84 TKK28 pKa = 10.37 SGRR31 pKa = 11.84 KK32 pKa = 8.29 IIARR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 9.96 GRR42 pKa = 11.84 KK43 pKa = 8.77 RR44 pKa = 11.84 LAAA47 pKa = 4.42
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.228
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.749
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.486
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1708
0
1708
511207
29
1605
299.3
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.363 ± 0.067
0.735 ± 0.022
5.367 ± 0.04
7.955 ± 0.069
5.049 ± 0.058
5.975 ± 0.057
1.413 ± 0.025
9.119 ± 0.056
10.006 ± 0.072
9.911 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.891 ± 0.026
4.935 ± 0.051
3.637 ± 0.039
2.746 ± 0.038
3.518 ± 0.038
5.565 ± 0.045
4.54 ± 0.035
7.07 ± 0.053
0.777 ± 0.022
4.43 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here