Rosellinia necatrix megabirnavirus 2-W8

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Megabirnaviridae; Megabirnavirus; unclassified Megabirnavirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U5BZK0|A0A0U5BZK0_9VIRU Putative coat protein OS=Rosellinia necatrix megabirnavirus 2-W8 OX=1676267 PE=4 SV=1
MM1 pKa = 7.27VLNSYY6 pKa = 7.92NTATVPKK13 pKa = 10.24VVVNRR18 pKa = 11.84HH19 pKa = 5.31AFYY22 pKa = 11.14QNTKK26 pKa = 9.97AKK28 pKa = 10.2DD29 pKa = 3.4YY30 pKa = 10.16SFRR33 pKa = 11.84FARR36 pKa = 11.84EE37 pKa = 3.44DD38 pKa = 3.58DD39 pKa = 3.81AVNTLVAQLEE49 pKa = 4.64TGVVQHH55 pKa = 6.1QVGDD59 pKa = 3.69MNYY62 pKa = 9.51NAGGLGSFSTILSGKK77 pKa = 10.08KK78 pKa = 9.66KK79 pKa = 9.05VTHH82 pKa = 4.95QQIGAFIDD90 pKa = 3.85QTGHH94 pKa = 5.62GAEE97 pKa = 4.34GSIHH101 pKa = 5.42STSTVKK107 pKa = 10.6QYY109 pKa = 9.56GTGKK113 pKa = 9.48EE114 pKa = 3.95VAGAITDD121 pKa = 3.56VRR123 pKa = 11.84NMLGGVYY130 pKa = 9.77DD131 pKa = 3.94RR132 pKa = 11.84KK133 pKa = 10.64HH134 pKa = 6.22PGVHH138 pKa = 6.86KK139 pKa = 10.7LGTLAATYY147 pKa = 10.43TDD149 pKa = 4.54DD150 pKa = 5.53APDD153 pKa = 3.63GLTPIFEE160 pKa = 4.28RR161 pKa = 11.84LVRR164 pKa = 11.84GAMHH168 pKa = 5.37TTAYY172 pKa = 9.63NAHH175 pKa = 6.68RR176 pKa = 11.84QPAPVAGVAPADD188 pKa = 3.64PMGYY192 pKa = 7.9TPKK195 pKa = 10.33QYY197 pKa = 10.51YY198 pKa = 9.28HH199 pKa = 7.42DD200 pKa = 3.95SRR202 pKa = 11.84RR203 pKa = 11.84IMPHH207 pKa = 5.44RR208 pKa = 11.84QVALVPQAANVMPHH222 pKa = 6.85ASLINNSAEE231 pKa = 4.07NILANMDD238 pKa = 3.66VNVTNEE244 pKa = 3.91LTRR247 pKa = 11.84TLLTISEE254 pKa = 4.46VPSFRR259 pKa = 11.84AAEE262 pKa = 3.95LRR264 pKa = 11.84DD265 pKa = 3.43IVAVLDD271 pKa = 3.91ACTKK275 pKa = 10.72ANLGFDD281 pKa = 3.4VHH283 pKa = 7.51ADD285 pKa = 3.28QMNEE289 pKa = 3.42HH290 pKa = 6.29STYY293 pKa = 7.83THH295 pKa = 5.95GVSRR299 pKa = 11.84VHH301 pKa = 7.29DD302 pKa = 3.96FLILDD307 pKa = 3.47MCNFYY312 pKa = 11.01SRR314 pKa = 11.84CFTDD318 pKa = 3.83EE319 pKa = 3.85AAANDD324 pKa = 3.77NGFEE328 pKa = 4.17NLNDD332 pKa = 4.1PAPNLIPLPANNGLQPGVALWFGFAGNAANRR363 pKa = 11.84TGVCNPRR370 pKa = 11.84SWMNAIAFLLEE381 pKa = 4.13LEE383 pKa = 4.78GGRR386 pKa = 11.84EE387 pKa = 3.85ACAIAFNNVVEE398 pKa = 4.19QTTRR402 pKa = 11.84FFGPNVTMLASPPVVRR418 pKa = 11.84ADD420 pKa = 3.47PDD422 pKa = 4.04TFGSAHH428 pKa = 5.9RR429 pKa = 11.84VLRR432 pKa = 11.84QRR434 pKa = 11.84LGWMYY439 pKa = 10.76CNIIRR444 pKa = 11.84TPNSAGDD451 pKa = 3.17AWLDD455 pKa = 3.51KK456 pKa = 10.84QHH458 pKa = 6.14NQWPTHH464 pKa = 5.91PKK466 pKa = 9.7LGRR469 pKa = 11.84YY470 pKa = 8.85DD471 pKa = 3.81DD472 pKa = 3.93RR473 pKa = 11.84AAAAGAGAANAATIDD488 pKa = 3.42QTLARR493 pKa = 11.84NLRR496 pKa = 11.84NEE498 pKa = 4.3LFNNAAAAAPGALEE512 pKa = 3.71NARR515 pKa = 11.84FDD517 pKa = 4.57FVWADD522 pKa = 3.51VAHH525 pKa = 6.62NGPGQAMLEE534 pKa = 4.13NMSGGVDD541 pKa = 3.65PLNDD545 pKa = 3.23QLVRR549 pKa = 11.84RR550 pKa = 11.84WGDD553 pKa = 3.26YY554 pKa = 10.86VPIFGTGVAAANPGEE569 pKa = 4.5QNTAPPLGPLTITEE583 pKa = 4.25VDD585 pKa = 3.62NFWWRR590 pKa = 11.84FFLNVSLEE598 pKa = 3.99SDD600 pKa = 3.5EE601 pKa = 4.38VVEE604 pKa = 4.99DD605 pKa = 4.58FLRR608 pKa = 11.84LMPSSLLSEE617 pKa = 4.05LLIWLTDD624 pKa = 3.41SEE626 pKa = 4.83LRR628 pKa = 11.84GGDD631 pKa = 3.26AFNVRR636 pKa = 11.84WEE638 pKa = 4.38VPAALQVVDD647 pKa = 4.33GRR649 pKa = 11.84RR650 pKa = 11.84MTLPIPGNKK659 pKa = 7.33QHH661 pKa = 6.3YY662 pKa = 9.52ANPAHH667 pKa = 6.16MRR669 pKa = 11.84NFAWVNRR676 pKa = 11.84EE677 pKa = 3.73EE678 pKa = 3.79VHH680 pKa = 6.02YY681 pKa = 10.87ASEE684 pKa = 4.42FVGNICVPAYY694 pKa = 9.51TGLAAFWGYY703 pKa = 11.32VKK705 pKa = 9.83DD706 pKa = 3.7TRR708 pKa = 11.84TGRR711 pKa = 11.84VTEE714 pKa = 4.24DD715 pKa = 3.74DD716 pKa = 3.42EE717 pKa = 5.08RR718 pKa = 11.84LAARR722 pKa = 11.84FRR724 pKa = 11.84VMTSDD729 pKa = 3.73KK730 pKa = 11.07LMSLAFALGGQMRR743 pKa = 11.84CAADD747 pKa = 3.4AMAMEE752 pKa = 5.3LSLCRR757 pKa = 11.84STIARR762 pKa = 11.84MQGHH766 pKa = 7.39ILTDD770 pKa = 3.58NAALDD775 pKa = 4.09AEE777 pKa = 4.89LVDD780 pKa = 3.7TDD782 pKa = 4.83VINAQQSMKK791 pKa = 10.52GYY793 pKa = 10.57RR794 pKa = 11.84NTCLAEE800 pKa = 3.97YY801 pKa = 10.54DD802 pKa = 3.89SLAVGMGIYY811 pKa = 10.6TMMGTYY817 pKa = 9.76SRR819 pKa = 11.84YY820 pKa = 9.27VEE822 pKa = 4.0CDD824 pKa = 2.87VRR826 pKa = 11.84FNGEE830 pKa = 3.56TWNLRR835 pKa = 11.84RR836 pKa = 11.84NRR838 pKa = 11.84QTAFAAYY845 pKa = 9.22RR846 pKa = 11.84CLHH849 pKa = 6.5PLMFEE854 pKa = 4.18YY855 pKa = 10.58FCPGANMGGGMLAGQTQVEE874 pKa = 4.46RR875 pKa = 11.84VEE877 pKa = 4.42SGCVKK882 pKa = 9.67TFPALFKK889 pKa = 10.96PEE891 pKa = 4.61DD892 pKa = 3.82DD893 pKa = 4.79SIIDD897 pKa = 3.75LEE899 pKa = 4.52IYY901 pKa = 10.38AAAQQYY907 pKa = 10.88AIIAGYY913 pKa = 9.68HH914 pKa = 5.99IDD916 pKa = 3.38YY917 pKa = 10.62TMRR920 pKa = 11.84SLVKK924 pKa = 9.81RR925 pKa = 11.84TGDD928 pKa = 3.14GTGAQFYY935 pKa = 10.6EE936 pKa = 4.59LFNPYY941 pKa = 9.47LRR943 pKa = 11.84HH944 pKa = 6.38QIRR947 pKa = 11.84TSDD950 pKa = 4.15CGPGSPLLAVSPLSFYY966 pKa = 11.4VSTFNVDD973 pKa = 3.32LFEE976 pKa = 4.47QEE978 pKa = 4.44GSFKK982 pKa = 10.66VAKK985 pKa = 10.2LDD987 pKa = 3.91LAQQTFIHH995 pKa = 6.33RR996 pKa = 11.84TMGWFATTCGDD1007 pKa = 3.48AGRR1010 pKa = 11.84PNAFPHH1016 pKa = 5.66GTQRR1020 pKa = 11.84LANSDD1025 pKa = 2.95IGVNRR1030 pKa = 11.84RR1031 pKa = 11.84FGAAWGAHH1039 pKa = 4.32GTMKK1043 pKa = 9.08PTWGMATQRR1052 pKa = 11.84RR1053 pKa = 11.84RR1054 pKa = 11.84NRR1056 pKa = 11.84DD1057 pKa = 3.07DD1058 pKa = 5.18RR1059 pKa = 11.84FDD1061 pKa = 3.9LVATRR1066 pKa = 11.84VFEE1069 pKa = 4.3IQDD1072 pKa = 2.93ATGQVRR1078 pKa = 11.84RR1079 pKa = 11.84EE1080 pKa = 3.71YY1081 pKa = 10.9SVYY1084 pKa = 9.79TNQGFALVHH1093 pKa = 6.52APHH1096 pKa = 6.92GWDD1099 pKa = 2.44TWVQSNRR1106 pKa = 11.84GLIFTQSYY1114 pKa = 9.14GAAQHH1119 pKa = 5.13QQVDD1123 pKa = 4.07RR1124 pKa = 11.84SASGFKK1130 pKa = 10.15FCRR1133 pKa = 11.84NRR1135 pKa = 11.84LTFRR1139 pKa = 11.84MAAMSDD1145 pKa = 3.54DD1146 pKa = 4.57YY1147 pKa = 11.06EE1148 pKa = 4.5FTMHH1152 pKa = 7.41PLAKK1156 pKa = 10.19AEE1158 pKa = 4.0LMPVEE1163 pKa = 4.45KK1164 pKa = 9.98MEE1166 pKa = 3.95MTAVVTVGEE1175 pKa = 4.88GEE1177 pKa = 4.26TQSRR1181 pKa = 11.84VSTSRR1186 pKa = 11.84MGSALSDD1193 pKa = 3.3MGGKK1197 pKa = 10.06RR1198 pKa = 11.84GDD1200 pKa = 3.17GGLRR1204 pKa = 11.84PRR1206 pKa = 11.84GPRR1209 pKa = 11.84TNVTPVEE1216 pKa = 4.12KK1217 pKa = 9.87PIPGTVEE1224 pKa = 3.76HH1225 pKa = 6.94LEE1227 pKa = 4.28VPLTAGTPASQAATLVPEE1245 pKa = 4.11QGDD1248 pKa = 3.8RR1249 pKa = 11.84VSDD1252 pKa = 3.75PLVPKK1257 pKa = 10.65NN1258 pKa = 3.56

Molecular weight: 138.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U5BZK0|A0A0U5BZK0_9VIRU Putative coat protein OS=Rosellinia necatrix megabirnavirus 2-W8 OX=1676267 PE=4 SV=1
MM1 pKa = 7.83ASVTKK6 pKa = 10.51KK7 pKa = 10.02LAKK10 pKa = 9.65VVCAIAAVRR19 pKa = 11.84QLHH22 pKa = 6.23RR23 pKa = 11.84MVDD26 pKa = 3.66LRR28 pKa = 11.84NVAMAARR35 pKa = 11.84CKK37 pKa = 9.68ATAEE41 pKa = 3.72WRR43 pKa = 11.84RR44 pKa = 11.84VLKK47 pKa = 10.79LVMCVMRR54 pKa = 11.84WQRR57 pKa = 11.84LVRR60 pKa = 11.84NSTSAASVTLSQLAEE75 pKa = 4.04QTTNAAGMGEE85 pKa = 4.28GDD87 pKa = 4.82VIWLAAIEE95 pKa = 4.2ALVSAVAVGPSVGAQXKK112 pKa = 9.17EE113 pKa = 4.03KK114 pKa = 10.68GVNQPGRR121 pKa = 11.84QLRR124 pKa = 11.84PVEE127 pKa = 4.4EE128 pKa = 4.34IGSLEE133 pKa = 4.05HH134 pKa = 5.69VAGVLRR140 pKa = 11.84SFLTWQRR147 pKa = 11.84YY148 pKa = 7.23RR149 pKa = 11.84RR150 pKa = 11.84IKK152 pKa = 9.7QLCFYY157 pKa = 8.3EE158 pKa = 4.16ARR160 pKa = 11.84RR161 pKa = 11.84IAAVRR166 pKa = 11.84NLRR169 pKa = 11.84IAVAARR175 pKa = 11.84DD176 pKa = 3.71EE177 pKa = 4.34AAATPGYY184 pKa = 9.41HH185 pKa = 3.59QTARR189 pKa = 11.84YY190 pKa = 7.64VHH192 pKa = 6.63SMLCKK197 pKa = 9.7RR198 pKa = 11.84AEE200 pKa = 4.3VPCTCGGLWVDD211 pKa = 3.59EE212 pKa = 4.89DD213 pKa = 4.35GAVLGEE219 pKa = 4.2AQAAAAFSSVQNQGTPRR236 pKa = 11.84FSPATHH242 pKa = 6.85TIVVALPVRR251 pKa = 11.84TEE253 pKa = 4.06SQRR256 pKa = 11.84SIAPNNQATAGDD268 pKa = 3.9RR269 pKa = 11.84QEE271 pKa = 5.34HH272 pKa = 5.63VGQLSMQEE280 pKa = 3.86LVAAYY285 pKa = 9.27IAEE288 pKa = 4.25HH289 pKa = 6.59RR290 pKa = 11.84SGVQAALRR298 pKa = 11.84MRR300 pKa = 11.84WQLPEE305 pKa = 4.44GVDD308 pKa = 3.27ANEE311 pKa = 4.08FVTAVAQRR319 pKa = 11.84LKK321 pKa = 11.01EE322 pKa = 4.03SDD324 pKa = 3.2QQ325 pKa = 3.44

Molecular weight: 35.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4221

325

1530

1055.3

115.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.888 ± 1.149

1.327 ± 0.126

5.33 ± 0.575

4.928 ± 0.215

3.435 ± 0.476

7.818 ± 0.477

2.464 ± 0.17

3.364 ± 0.107

2.227 ± 0.45

8.742 ± 0.613

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.253

3.909 ± 0.628

4.454 ± 0.305

4.241 ± 0.382

7.865 ± 0.422

5.354 ± 0.769

6.207 ± 0.753

7.771 ± 0.612

1.943 ± 0.147

2.535 ± 0.398
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski