Poriferisphaera corsica

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae;

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517YR49|A0A517YR49_9BACT PEP-CTERM domain-containing protein OS=Poriferisphaera corsica OX=2528020 GN=KS4_07330 PE=4 SV=1
MM1 pKa = 7.34GFKK4 pKa = 10.39FGEE7 pKa = 4.06LCADD11 pKa = 3.7IFADD15 pKa = 3.74VRR17 pKa = 11.84EE18 pKa = 4.3CVKK21 pKa = 10.48LGRR24 pKa = 11.84YY25 pKa = 7.82AWCTLVVVCEE35 pKa = 4.28LGFEE39 pKa = 4.4FVFVEE44 pKa = 4.49LGEE47 pKa = 4.44SAISVPDD54 pKa = 4.23DD55 pKa = 3.39EE56 pKa = 6.28DD57 pKa = 3.54VLNFSGFDD65 pKa = 3.65GKK67 pKa = 10.7DD68 pKa = 2.99KK69 pKa = 11.35GSDD72 pKa = 3.36DD73 pKa = 4.02VFGDD77 pKa = 4.03DD78 pKa = 4.31VAGISDD84 pKa = 4.28DD85 pKa = 3.51VCLAVLEE92 pKa = 4.41AEE94 pKa = 4.47RR95 pKa = 11.84FFDD98 pKa = 4.29GDD100 pKa = 4.09SAIHH104 pKa = 6.8AGDD107 pKa = 3.16NCGIEE112 pKa = 4.1WGCDD116 pKa = 3.26CFF118 pKa = 5.57

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517YQZ5|A0A517YQZ5_9BACT Uncharacterized protein OS=Poriferisphaera corsica OX=2528020 GN=KS4_06710 PE=4 SV=1
MM1 pKa = 6.74WWFVRR6 pKa = 11.84LHH8 pKa = 6.22ALIIAAKK15 pKa = 8.13SNQMIRR21 pKa = 11.84SAITVARR28 pKa = 11.84GTLRR32 pKa = 11.84SSKK35 pKa = 9.89AQKK38 pKa = 9.81PVIVAALIHH47 pKa = 6.32LMSIILQQVGMIRR60 pKa = 11.84QQ61 pKa = 3.32

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3659

0

3659

1197420

29

2861

327.3

36.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.455 ± 0.049

1.084 ± 0.016

6.066 ± 0.034

6.153 ± 0.05

3.725 ± 0.031

7.658 ± 0.058

2.265 ± 0.023

6.547 ± 0.041

5.084 ± 0.043

9.374 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.834 ± 0.025

4.283 ± 0.041

4.231 ± 0.031

3.966 ± 0.033

5.181 ± 0.042

6.054 ± 0.033

5.662 ± 0.048

6.905 ± 0.05

1.4 ± 0.017

3.072 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski