Phanerochaete carnosa (strain HHB-10118-sp) (White-rot fungus) (Peniophora carnosa)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Phanerochaetaceae; Phanerochaete; Phanerochaete carnosa

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13867 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K5W9S3|K5W9S3_PHACS Glycoside hydrolase family 16 protein OS=Phanerochaete carnosa (strain HHB-10118-sp) OX=650164 GN=PHACADRAFT_246779 PE=4 SV=1
MM1 pKa = 7.47PAIRR5 pKa = 11.84LLSTTLFAAGTLAASLPSLASLQASSGICGSVGEE39 pKa = 4.14VSLNKK44 pKa = 9.92IEE46 pKa = 4.18VWSGATKK53 pKa = 10.61LGFLAPDD60 pKa = 3.36VSGLLGGGYY69 pKa = 10.5GIISPDD75 pKa = 3.21TSDD78 pKa = 3.66ALTGVFTCGAPTNPVTMGAANAQTVPGTNVNAIGLIAPYY117 pKa = 9.42YY118 pKa = 9.0PNGGSPDD125 pKa = 3.7LASGGSNWLALGATYY140 pKa = 10.59VSPSGAKK147 pKa = 9.89PNATGITFGDD157 pKa = 3.58SQAWFEE163 pKa = 4.45SSVWTTSGGYY173 pKa = 9.86DD174 pKa = 3.65GNGNYY179 pKa = 9.58HH180 pKa = 7.58DD181 pKa = 5.18YY182 pKa = 10.62ILHH185 pKa = 6.17EE186 pKa = 4.76SEE188 pKa = 4.53SHH190 pKa = 4.92WVNSDD195 pKa = 2.68GDD197 pKa = 4.26LPSLLLAALPDD208 pKa = 3.3QGGIVVTGDD217 pKa = 3.26FNAFADD223 pKa = 3.9SYY225 pKa = 10.89SYY227 pKa = 10.57VGNWVEE233 pKa = 4.17VQLASAPASDD243 pKa = 4.44RR244 pKa = 11.84GGPPPQATLVAAVAIINPLGAVDD267 pKa = 5.78SDD269 pKa = 5.46DD270 pKa = 6.09SDD272 pKa = 6.11DD273 pKa = 6.07DD274 pKa = 5.25DD275 pKa = 4.44DD276 pKa = 7.04ASVNEE281 pKa = 4.27HH282 pKa = 6.1RR283 pKa = 11.84QGSLDD288 pKa = 3.71AADD291 pKa = 3.78SDD293 pKa = 3.92VGVRR297 pKa = 11.84DD298 pKa = 3.54RR299 pKa = 11.84EE300 pKa = 4.21RR301 pKa = 11.84RR302 pKa = 11.84CAAAVVHH309 pKa = 6.6LVLL312 pKa = 5.72

Molecular weight: 31.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K5WED9|K5WED9_PHACS Uncharacterized protein OS=Phanerochaete carnosa (strain HHB-10118-sp) OX=650164 GN=PHACADRAFT_206348 PE=4 SV=1
MM1 pKa = 6.84VRR3 pKa = 11.84AARR6 pKa = 11.84LVLILPRR13 pKa = 11.84TVPRR17 pKa = 11.84SVPGTTTRR25 pKa = 11.84PIVRR29 pKa = 11.84GSTTSSAPASTTPATTSTMPASASRR54 pKa = 11.84VV55 pKa = 3.47

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13867

0

13867

5321648

49

3931

383.8

42.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.159 ± 0.018

1.335 ± 0.009

5.547 ± 0.014

5.907 ± 0.022

3.74 ± 0.014

6.479 ± 0.017

2.6 ± 0.011

4.669 ± 0.013

4.256 ± 0.022

9.428 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.007

3.191 ± 0.012

6.404 ± 0.023

3.755 ± 0.015

6.32 ± 0.018

8.312 ± 0.027

5.979 ± 0.015

6.627 ± 0.016

1.489 ± 0.008

2.706 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski