Amino acid dipepetide frequency for Flexilinea flocculi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.207AlaAla: 7.207 ± 0.113
0.932AlaCys: 0.932 ± 0.042
4.199AlaAsp: 4.199 ± 0.079
4.806AlaGlu: 4.806 ± 0.077
3.478AlaPhe: 3.478 ± 0.07
5.617AlaGly: 5.617 ± 0.132
1.142AlaHis: 1.142 ± 0.037
6.161AlaIle: 6.161 ± 0.099
3.659AlaLys: 3.659 ± 0.068
6.918AlaLeu: 6.918 ± 0.102
2.043AlaMet: 2.043 ± 0.051
2.701AlaAsn: 2.701 ± 0.067
2.072AlaPro: 2.072 ± 0.047
2.333AlaGln: 2.333 ± 0.053
2.79AlaArg: 2.79 ± 0.064
4.483AlaSer: 4.483 ± 0.114
2.889AlaThr: 2.889 ± 0.089
5.484AlaVal: 5.484 ± 0.098
0.819AlaTrp: 0.819 ± 0.031
2.533AlaTyr: 2.533 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.801CysAla: 0.801 ± 0.039
0.214CysCys: 0.214 ± 0.017
0.654CysAsp: 0.654 ± 0.031
0.658CysGlu: 0.658 ± 0.024
0.635CysPhe: 0.635 ± 0.03
1.209CysGly: 1.209 ± 0.043
0.264CysHis: 0.264 ± 0.02
0.912CysIle: 0.912 ± 0.033
0.515CysLys: 0.515 ± 0.026
1.125CysLeu: 1.125 ± 0.044
0.284CysMet: 0.284 ± 0.02
0.548CysAsn: 0.548 ± 0.026
0.614CysPro: 0.614 ± 0.03
0.372CysGln: 0.372 ± 0.02
0.575CysArg: 0.575 ± 0.028
0.923CysSer: 0.923 ± 0.036
0.597CysThr: 0.597 ± 0.029
0.711CysVal: 0.711 ± 0.029
0.159CysTrp: 0.159 ± 0.013
0.419CysTyr: 0.419 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.801AspAla: 3.801 ± 0.07
0.666AspCys: 0.666 ± 0.031
2.616AspAsp: 2.616 ± 0.073
3.725AspGlu: 3.725 ± 0.085
2.926AspPhe: 2.926 ± 0.05
3.72AspGly: 3.72 ± 0.124
1.194AspHis: 1.194 ± 0.039
4.046AspIle: 4.046 ± 0.068
2.108AspLys: 2.108 ± 0.055
5.421AspLeu: 5.421 ± 0.077
1.237AspMet: 1.237 ± 0.04
1.883AspAsn: 1.883 ± 0.069
2.713AspPro: 2.713 ± 0.05
2.481AspGln: 2.481 ± 0.053
2.597AspArg: 2.597 ± 0.062
3.417AspSer: 3.417 ± 0.09
2.647AspThr: 2.647 ± 0.163
3.293AspVal: 3.293 ± 0.071
0.816AspTrp: 0.816 ± 0.032
2.199AspTyr: 2.199 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
4.316GluAla: 4.316 ± 0.082
0.49GluCys: 0.49 ± 0.025
2.934GluAsp: 2.934 ± 0.053
4.127GluGlu: 4.127 ± 0.113
2.431GluPhe: 2.431 ± 0.06
3.373GluGly: 3.373 ± 0.06
0.92GluHis: 0.92 ± 0.035
6.195GluIle: 6.195 ± 0.106
5.675GluLys: 5.675 ± 0.126
5.199GluLeu: 5.199 ± 0.094
1.962GluMet: 1.962 ± 0.055
4.013GluAsn: 4.013 ± 0.07
2.118GluPro: 2.118 ± 0.056
2.229GluGln: 2.229 ± 0.056
2.742GluArg: 2.742 ± 0.076
3.772GluSer: 3.772 ± 0.06
3.584GluThr: 3.584 ± 0.066
3.343GluVal: 3.343 ± 0.073
0.756GluTrp: 0.756 ± 0.032
1.951GluTyr: 1.951 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.162PheAla: 3.162 ± 0.068
0.782PheCys: 0.782 ± 0.033
2.696PheAsp: 2.696 ± 0.057
2.689PheGlu: 2.689 ± 0.058
3.077PhePhe: 3.077 ± 0.096
3.504PheGly: 3.504 ± 0.073
1.135PheHis: 1.135 ± 0.04
3.695PheIle: 3.695 ± 0.073
1.652PheLys: 1.652 ± 0.045
5.47PheLeu: 5.47 ± 0.132
1.024PheMet: 1.024 ± 0.036
1.907PheAsn: 1.907 ± 0.074
2.191PhePro: 2.191 ± 0.065
1.963PheGln: 1.963 ± 0.047
2.103PheArg: 2.103 ± 0.056
4.542PheSer: 4.542 ± 0.078
2.508PheThr: 2.508 ± 0.058
2.96PheVal: 2.96 ± 0.063
0.692PheTrp: 0.692 ± 0.03
1.848PheTyr: 1.848 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.443GlyAla: 4.443 ± 0.112
0.967GlyCys: 0.967 ± 0.043
3.15GlyAsp: 3.15 ± 0.105
3.696GlyGlu: 3.696 ± 0.064
3.7GlyPhe: 3.7 ± 0.061
4.696GlyGly: 4.696 ± 0.254
1.113GlyHis: 1.113 ± 0.035
6.635GlyIle: 6.635 ± 0.09
4.711GlyLys: 4.711 ± 0.083
6.542GlyLeu: 6.542 ± 0.092
2.108GlyMet: 2.108 ± 0.05
3.156GlyAsn: 3.156 ± 0.146
1.654GlyPro: 1.654 ± 0.043
2.12GlyGln: 2.12 ± 0.053
2.662GlyArg: 2.662 ± 0.059
4.711GlySer: 4.711 ± 0.221
4.106GlyThr: 4.106 ± 0.197
4.231GlyVal: 4.231 ± 0.099
1.014GlyTrp: 1.014 ± 0.034
2.694GlyTyr: 2.694 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.267HisAla: 1.267 ± 0.036
0.321HisCys: 0.321 ± 0.021
0.838HisAsp: 0.838 ± 0.03
1.068HisGlu: 1.068 ± 0.039
1.074HisPhe: 1.074 ± 0.035
1.302HisGly: 1.302 ± 0.039
0.503HisHis: 0.503 ± 0.028
1.386HisIle: 1.386 ± 0.038
0.747HisLys: 0.747 ± 0.029
1.786HisLeu: 1.786 ± 0.044
0.378HisMet: 0.378 ± 0.02
0.721HisAsn: 0.721 ± 0.026
1.152HisPro: 1.152 ± 0.041
0.85HisGln: 0.85 ± 0.033
0.865HisArg: 0.865 ± 0.033
1.101HisSer: 1.101 ± 0.037
0.907HisThr: 0.907 ± 0.03
1.027HisVal: 1.027 ± 0.034
0.286HisTrp: 0.286 ± 0.017
0.654HisTyr: 0.654 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.408IleAla: 6.408 ± 0.094
1.21IleCys: 1.21 ± 0.038
4.691IleAsp: 4.691 ± 0.09
4.894IleGlu: 4.894 ± 0.07
4.209IlePhe: 4.209 ± 0.087
6.045IleGly: 6.045 ± 0.112
1.836IleHis: 1.836 ± 0.048
6.422IleIle: 6.422 ± 0.099
3.554IleLys: 3.554 ± 0.062
9.11IleLeu: 9.11 ± 0.14
1.658IleMet: 1.658 ± 0.05
3.555IleAsn: 3.555 ± 0.103
4.669IlePro: 4.669 ± 0.1
3.653IleGln: 3.653 ± 0.064
4.441IleArg: 4.441 ± 0.097
6.454IleSer: 6.454 ± 0.092
4.572IleThr: 4.572 ± 0.107
4.895IleVal: 4.895 ± 0.085
0.927IleTrp: 0.927 ± 0.037
2.684IleTyr: 2.684 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
3.908LysAla: 3.908 ± 0.078
0.398LysCys: 0.398 ± 0.019
2.817LysAsp: 2.817 ± 0.074
3.928LysGlu: 3.928 ± 0.085
1.975LysPhe: 1.975 ± 0.055
3.12LysGly: 3.12 ± 0.068
0.769LysHis: 0.769 ± 0.026
5.469LysIle: 5.469 ± 0.101
4.542LysLys: 4.542 ± 0.117
4.316LysLeu: 4.316 ± 0.093
1.769LysMet: 1.769 ± 0.051
3.494LysAsn: 3.494 ± 0.077
2.31LysPro: 2.31 ± 0.063
1.957LysGln: 1.957 ± 0.057
2.512LysArg: 2.512 ± 0.07
3.273LysSer: 3.273 ± 0.062
3.46LysThr: 3.46 ± 0.065
2.978LysVal: 2.978 ± 0.064
0.64LysTrp: 0.64 ± 0.027
1.737LysTyr: 1.737 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
6.905LeuAla: 6.905 ± 0.087
1.273LeuCys: 1.273 ± 0.044
5.084LeuAsp: 5.084 ± 0.088
5.475LeuGlu: 5.475 ± 0.101
5.38LeuPhe: 5.38 ± 0.124
5.956LeuGly: 5.956 ± 0.092
1.616LeuHis: 1.616 ± 0.041
8.369LeuIle: 8.369 ± 0.128
5.378LeuLys: 5.378 ± 0.103
10.063LeuLeu: 10.063 ± 0.19
2.451LeuMet: 2.451 ± 0.063
4.245LeuAsn: 4.245 ± 0.088
4.502LeuPro: 4.502 ± 0.102
3.571LeuGln: 3.571 ± 0.076
4.212LeuArg: 4.212 ± 0.097
7.708LeuSer: 7.708 ± 0.115
5.422LeuThr: 5.422 ± 0.116
5.306LeuVal: 5.306 ± 0.091
1.124LeuTrp: 1.124 ± 0.042
2.949LeuTyr: 2.949 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.962MetAla: 1.962 ± 0.051
0.198MetCys: 0.198 ± 0.015
1.598MetAsp: 1.598 ± 0.043
1.707MetGlu: 1.707 ± 0.051
0.817MetPhe: 0.817 ± 0.032
1.732MetGly: 1.732 ± 0.051
0.337MetHis: 0.337 ± 0.02
2.552MetIle: 2.552 ± 0.061
1.922MetLys: 1.922 ± 0.052
2.348MetLeu: 2.348 ± 0.064
0.799MetMet: 0.799 ± 0.031
1.545MetAsn: 1.545 ± 0.039
0.979MetPro: 0.979 ± 0.038
0.904MetGln: 0.904 ± 0.038
1.136MetArg: 1.136 ± 0.041
1.408MetSer: 1.408 ± 0.046
1.567MetThr: 1.567 ± 0.046
1.584MetVal: 1.584 ± 0.043
0.156MetTrp: 0.156 ± 0.012
0.582MetTyr: 0.582 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.18AsnAla: 3.18 ± 0.083
0.567AsnCys: 0.567 ± 0.027
2.299AsnAsp: 2.299 ± 0.096
2.732AsnGlu: 2.732 ± 0.062
2.099AsnPhe: 2.099 ± 0.045
3.367AsnGly: 3.367 ± 0.125
1.12AsnHis: 1.12 ± 0.04
3.377AsnIle: 3.377 ± 0.094
1.924AsnLys: 1.924 ± 0.054
4.714AsnLeu: 4.714 ± 0.09
1.0AsnMet: 1.0 ± 0.032
1.92AsnAsn: 1.92 ± 0.066
2.822AsnPro: 2.822 ± 0.058
2.274AsnGln: 2.274 ± 0.052
2.403AsnArg: 2.403 ± 0.057
2.972AsnSer: 2.972 ± 0.099
2.361AsnThr: 2.361 ± 0.103
2.645AsnVal: 2.645 ± 0.086
0.674AsnTrp: 0.674 ± 0.024
1.689AsnTyr: 1.689 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.025ProAla: 3.025 ± 0.064
0.409ProCys: 0.409 ± 0.022
3.008ProAsp: 3.008 ± 0.063
3.556ProGlu: 3.556 ± 0.075
2.087ProPhe: 2.087 ± 0.059
2.91ProGly: 2.91 ± 0.064
0.681ProHis: 0.681 ± 0.027
3.139ProIle: 3.139 ± 0.066
1.866ProLys: 1.866 ± 0.06
3.653ProLeu: 3.653 ± 0.079
1.004ProMet: 1.004 ± 0.042
1.798ProAsn: 1.798 ± 0.046
1.241ProPro: 1.241 ± 0.049
1.486ProGln: 1.486 ± 0.042
1.393ProArg: 1.393 ± 0.041
2.615ProSer: 2.615 ± 0.054
2.296ProThr: 2.296 ± 0.072
3.425ProVal: 3.425 ± 0.073
0.538ProTrp: 0.538 ± 0.025
1.658ProTyr: 1.658 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
2.651GlnAla: 2.651 ± 0.049
0.335GlnCys: 0.335 ± 0.018
1.762GlnAsp: 1.762 ± 0.045
2.407GlnGlu: 2.407 ± 0.066
1.568GlnPhe: 1.568 ± 0.039
1.943GlnGly: 1.943 ± 0.047
0.614GlnHis: 0.614 ± 0.031
3.637GlnIle: 3.637 ± 0.066
3.026GlnLys: 3.026 ± 0.078
3.167GlnLeu: 3.167 ± 0.069
1.165GlnMet: 1.165 ± 0.041
2.314GlnAsn: 2.314 ± 0.047
1.547GlnPro: 1.547 ± 0.046
1.597GlnGln: 1.597 ± 0.053
1.734GlnArg: 1.734 ± 0.058
2.453GlnSer: 2.453 ± 0.06
2.235GlnThr: 2.235 ± 0.049
2.05GlnVal: 2.05 ± 0.043
0.477GlnTrp: 0.477 ± 0.022
1.278GlnTyr: 1.278 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.539ArgAla: 2.539 ± 0.065
0.556ArgCys: 0.556 ± 0.027
2.196ArgAsp: 2.196 ± 0.058
2.835ArgGlu: 2.835 ± 0.079
2.377ArgPhe: 2.377 ± 0.078
2.323ArgGly: 2.323 ± 0.051
0.77ArgHis: 0.77 ± 0.033
4.294ArgIle: 4.294 ± 0.071
3.161ArgLys: 3.161 ± 0.086
4.258ArgLeu: 4.258 ± 0.093
1.371ArgMet: 1.371 ± 0.044
2.394ArgAsn: 2.394 ± 0.053
1.518ArgPro: 1.518 ± 0.045
1.681ArgGln: 1.681 ± 0.054
1.986ArgArg: 1.986 ± 0.06
2.725ArgSer: 2.725 ± 0.069
2.247ArgThr: 2.247 ± 0.047
2.466ArgVal: 2.466 ± 0.054
0.603ArgTrp: 0.603 ± 0.027
1.737ArgTyr: 1.737 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
4.89SerAla: 4.89 ± 0.136
0.813SerCys: 0.813 ± 0.036
3.928SerAsp: 3.928 ± 0.083
4.375SerGlu: 4.375 ± 0.078
3.529SerPhe: 3.529 ± 0.065
5.815SerGly: 5.815 ± 0.256
1.131SerHis: 1.131 ± 0.033
5.853SerIle: 5.853 ± 0.105
3.311SerLys: 3.311 ± 0.066
6.434SerLeu: 6.434 ± 0.084
1.746SerMet: 1.746 ± 0.043
2.797SerAsn: 2.797 ± 0.082
2.512SerPro: 2.512 ± 0.059
2.361SerGln: 2.361 ± 0.045
2.809SerArg: 2.809 ± 0.074
4.464SerSer: 4.464 ± 0.133
3.249SerThr: 3.249 ± 0.108
4.667SerVal: 4.667 ± 0.098
0.9SerTrp: 0.9 ± 0.031
2.284SerTyr: 2.284 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
4.689ThrAla: 4.689 ± 0.147
0.592ThrCys: 0.592 ± 0.027
3.036ThrAsp: 3.036 ± 0.11
3.176ThrGlu: 3.176 ± 0.057
2.364ThrPhe: 2.364 ± 0.089
4.505ThrGly: 4.505 ± 0.209
0.893ThrHis: 0.893 ± 0.031
4.7ThrIle: 4.7 ± 0.111
2.422ThrLys: 2.422 ± 0.054
5.05ThrLeu: 5.05 ± 0.133
1.215ThrMet: 1.215 ± 0.044
2.268ThrAsn: 2.268 ± 0.074
2.594ThrPro: 2.594 ± 0.074
1.602ThrGln: 1.602 ± 0.044
2.101ThrArg: 2.101 ± 0.048
3.232ThrSer: 3.232 ± 0.114
2.771ThrThr: 2.771 ± 0.114
3.718ThrVal: 3.718 ± 0.091
0.704ThrTrp: 0.704 ± 0.03
1.733ThrTyr: 1.733 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
3.93ValAla: 3.93 ± 0.07
0.822ValCys: 0.822 ± 0.032
3.394ValAsp: 3.394 ± 0.061
3.596ValGlu: 3.596 ± 0.072
3.448ValPhe: 3.448 ± 0.068
3.703ValGly: 3.703 ± 0.084
1.1ValHis: 1.1 ± 0.037
5.536ValIle: 5.536 ± 0.081
3.028ValLys: 3.028 ± 0.07
6.529ValLeu: 6.529 ± 0.083
1.549ValMet: 1.549 ± 0.044
2.738ValAsn: 2.738 ± 0.076
2.544ValPro: 2.544 ± 0.054
2.081ValGln: 2.081 ± 0.05
2.618ValArg: 2.618 ± 0.056
4.41ValSer: 4.41 ± 0.091
3.436ValThr: 3.436 ± 0.11
4.115ValVal: 4.115 ± 0.082
0.71ValTrp: 0.71 ± 0.025
2.134ValTyr: 2.134 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.746TrpAla: 0.746 ± 0.03
0.123TrpCys: 0.123 ± 0.011
0.656TrpAsp: 0.656 ± 0.025
0.712TrpGlu: 0.712 ± 0.029
0.597TrpPhe: 0.597 ± 0.028
0.786TrpGly: 0.786 ± 0.027
0.25TrpHis: 0.25 ± 0.015
1.31TrpIle: 1.31 ± 0.043
0.761TrpLys: 0.761 ± 0.028
1.424TrpLeu: 1.424 ± 0.047
0.436TrpMet: 0.436 ± 0.024
0.772TrpAsn: 0.772 ± 0.03
0.403TrpPro: 0.403 ± 0.022
0.566TrpGln: 0.566 ± 0.028
0.528TrpArg: 0.528 ± 0.022
0.671TrpSer: 0.671 ± 0.029
0.687TrpThr: 0.687 ± 0.024
0.69TrpVal: 0.69 ± 0.027
0.18TrpTrp: 0.18 ± 0.013
0.397TrpTyr: 0.397 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.439TyrAla: 2.439 ± 0.05
0.52TyrCys: 0.52 ± 0.025
1.948TyrAsp: 1.948 ± 0.055
1.965TyrGlu: 1.965 ± 0.047
1.832TyrPhe: 1.832 ± 0.051
2.483TyrGly: 2.483 ± 0.056
0.844TyrHis: 0.844 ± 0.032
2.2TyrIle: 2.2 ± 0.067
1.41TyrLys: 1.41 ± 0.042
3.462TyrLeu: 3.462 ± 0.07
0.634TyrMet: 0.634 ± 0.027
1.37TyrAsn: 1.37 ± 0.042
1.634TyrPro: 1.634 ± 0.049
1.878TyrGln: 1.878 ± 0.052
1.827TyrArg: 1.827 ± 0.047
2.537TyrSer: 2.537 ± 0.051
1.824TyrThr: 1.824 ± 0.071
1.833TyrVal: 1.833 ± 0.048
0.518TyrTrp: 0.518 ± 0.026
1.457TyrTyr: 1.457 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2774 proteins (989891 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski