Acidiferrobacter thiooxydans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Acidiferrobacterales; Acidiferrobacteraceae; Acidiferrobacter

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C2FXT2|A0A1C2FXT2_9GAMM 50S ribosomal protein L22 OS=Acidiferrobacter thiooxydans OX=163359 GN=rplV PE=3 SV=1
MM1 pKa = 7.75SDD3 pKa = 3.24DD4 pKa = 3.25QRR6 pKa = 11.84IYY8 pKa = 10.17MEE10 pKa = 4.59TIVEE14 pKa = 4.01YY15 pKa = 10.66MEE17 pKa = 5.27DD18 pKa = 3.84CGDD21 pKa = 3.45GTGIHH26 pKa = 6.28HH27 pKa = 6.94VLYY30 pKa = 10.72FCDD33 pKa = 4.26DD34 pKa = 3.41SGTFILEE41 pKa = 3.96STGVDD46 pKa = 3.74FHH48 pKa = 7.29EE49 pKa = 4.94GTEE52 pKa = 3.99IEE54 pKa = 4.6DD55 pKa = 4.16FNDD58 pKa = 3.24EE59 pKa = 5.08EE60 pKa = 4.13ALEE63 pKa = 4.25WCVEE67 pKa = 4.21FVGMEE72 pKa = 3.97YY73 pKa = 10.84VEE75 pKa = 3.93AARR78 pKa = 11.84RR79 pKa = 11.84IIGADD84 pKa = 3.26DD85 pKa = 3.53EE86 pKa = 4.25ARR88 pKa = 11.84AWIVGHH94 pKa = 6.91GITEE98 pKa = 4.16VEE100 pKa = 4.21LL101 pKa = 4.41

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C2G262|A0A1C2G262_9GAMM Uncharacterized protein OS=Acidiferrobacter thiooxydans OX=163359 GN=A9R16_10615 PE=4 SV=1
MM1 pKa = 7.61INQPRR6 pKa = 11.84IRR8 pKa = 11.84ILVGVIALGLATTAFASPASVALSQAVKK36 pKa = 10.49KK37 pKa = 10.12GAHH40 pKa = 6.22IFNTDD45 pKa = 2.49SFGSHH50 pKa = 5.87EE51 pKa = 4.16PSFHH55 pKa = 6.37GRR57 pKa = 11.84HH58 pKa = 4.82TTCSSCHH65 pKa = 5.8IDD67 pKa = 3.07GGRR70 pKa = 11.84VMGRR74 pKa = 11.84LPNGKK79 pKa = 9.7RR80 pKa = 11.84IPSLVNAAAIFPRR93 pKa = 11.84YY94 pKa = 9.28SPKK97 pKa = 9.46LHH99 pKa = 6.55KK100 pKa = 10.75VITLEE105 pKa = 3.94TQIRR109 pKa = 11.84HH110 pKa = 5.3CVANGLMGHH119 pKa = 6.79PPAYY123 pKa = 10.06GSQTMADD130 pKa = 3.56LVSYY134 pKa = 10.39LGSLAHH140 pKa = 6.3GQRR143 pKa = 11.84IMIGGKK149 pKa = 8.93PRR151 pKa = 3.36

Molecular weight: 16.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2872

0

2872

873618

26

5430

304.2

33.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.708 ± 0.059

1.033 ± 0.017

5.11 ± 0.037

5.148 ± 0.055

3.439 ± 0.03

8.587 ± 0.055

2.733 ± 0.029

4.766 ± 0.032

2.688 ± 0.042

10.748 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.25 ± 0.025

2.276 ± 0.042

5.327 ± 0.041

3.117 ± 0.025

8.3 ± 0.071

4.808 ± 0.053

5.194 ± 0.057

7.712 ± 0.039

1.418 ± 0.02

2.636 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski