Haloarchaeobius iranensis

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Haloarchaeobius

Average proteome isoelectric point is 4.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9XM11|A0A1G9XM11_9EURY PD-(D/E)XK endonuclease OS=Haloarchaeobius iranensis OX=996166 GN=SAMN05192554_11130 PE=4 SV=1
MM1 pKa = 7.43VRR3 pKa = 11.84DD4 pKa = 3.76TNGVSRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.46LLKK15 pKa = 10.65GVGAGLAATTMAGEE29 pKa = 4.49VVAGEE34 pKa = 4.37GAQDD38 pKa = 3.81VIVGVSDD45 pKa = 3.73DD46 pKa = 4.84HH47 pKa = 6.84GLQTARR53 pKa = 11.84EE54 pKa = 4.08RR55 pKa = 11.84ASSVRR60 pKa = 11.84HH61 pKa = 5.6EE62 pKa = 4.72FDD64 pKa = 4.28FDD66 pKa = 5.38DD67 pKa = 4.31IGQAVAGRR75 pKa = 11.84FPAEE79 pKa = 5.21AIDD82 pKa = 4.03ALQNNPHH89 pKa = 5.03VRR91 pKa = 11.84YY92 pKa = 9.84VEE94 pKa = 3.96RR95 pKa = 11.84DD96 pKa = 3.47GEE98 pKa = 4.39YY99 pKa = 10.32HH100 pKa = 7.3ALAQTLPWGVDD111 pKa = 2.99RR112 pKa = 11.84VDD114 pKa = 4.03ADD116 pKa = 3.8VLHH119 pKa = 6.92GNGDD123 pKa = 3.83TASGADD129 pKa = 3.48IAILDD134 pKa = 3.74TGIDD138 pKa = 3.59SDD140 pKa = 5.41HH141 pKa = 7.65PDD143 pKa = 3.62LADD146 pKa = 4.16NIQGGKK152 pKa = 10.14CFTDD156 pKa = 3.5NCCGEE161 pKa = 4.25AGGGGGPFGCDD172 pKa = 2.95TNTNDD177 pKa = 5.83CPNAWDD183 pKa = 4.92DD184 pKa = 4.64DD185 pKa = 4.42NDD187 pKa = 4.45HH188 pKa = 6.37GTHH191 pKa = 6.69CAGIADD197 pKa = 3.94AVDD200 pKa = 3.41NTEE203 pKa = 3.96GVVGVSTQANLWAGKK218 pKa = 10.17VLDD221 pKa = 4.26GCGSGSLSAIAAGIEE236 pKa = 3.76WAADD240 pKa = 3.16QGFDD244 pKa = 3.67VASMSLGASSGDD256 pKa = 3.32QTLQDD261 pKa = 3.64AVQYY265 pKa = 10.41AANQGLLMVAAAGNDD280 pKa = 4.51GPCSDD285 pKa = 4.28CVGYY289 pKa = 8.77PAAYY293 pKa = 8.96PEE295 pKa = 4.5VIAVSSTASDD305 pKa = 3.9DD306 pKa = 3.88SLSDD310 pKa = 3.89FSSTGPEE317 pKa = 3.93VEE319 pKa = 4.35LAAPGTDD326 pKa = 2.67IYY328 pKa = 10.94STVPSGYY335 pKa = 8.66ATFSGTSMACPHH347 pKa = 6.06VSGAAAQVMATGATASEE364 pKa = 4.34ARR366 pKa = 11.84TILQDD371 pKa = 3.36SAEE374 pKa = 4.84DD375 pKa = 3.08IGLAGNEE382 pKa = 3.95QGYY385 pKa = 9.34GLVDD389 pKa = 3.64AEE391 pKa = 4.41NAVAAAGGGDD401 pKa = 3.91GGDD404 pKa = 3.7AAPSVSWANPAGGDD418 pKa = 3.83TVSDD422 pKa = 3.7TVSIQLSASDD432 pKa = 5.48AEE434 pKa = 4.78DD435 pKa = 3.83SDD437 pKa = 5.24DD438 pKa = 3.93SLIVEE443 pKa = 4.5WSVDD447 pKa = 3.25GGPAQTASYY456 pKa = 10.44DD457 pKa = 3.83STSGYY462 pKa = 10.84YY463 pKa = 8.76EE464 pKa = 4.55ASWDD468 pKa = 3.74STGVADD474 pKa = 5.44GDD476 pKa = 4.07HH477 pKa = 6.38TLSATATDD485 pKa = 3.72AAGNTSSSSITVTTDD500 pKa = 2.71NSSSGSTAPAIDD512 pKa = 3.78EE513 pKa = 4.17FAVTNNSNGGWARR526 pKa = 11.84FDD528 pKa = 3.64VDD530 pKa = 3.69WAVSDD535 pKa = 4.33ADD537 pKa = 3.85GDD539 pKa = 4.18LASVDD544 pKa = 4.65LVLDD548 pKa = 3.72QNGTADD554 pKa = 3.61SASDD558 pKa = 3.77SVSGSSASGSTRR570 pKa = 11.84LQDD573 pKa = 3.52KK574 pKa = 10.09KK575 pKa = 11.29GSGSYY580 pKa = 10.84DD581 pKa = 3.19VTLTVIDD588 pKa = 3.86AAGNTTSQTKK598 pKa = 8.34TVTAA602 pKa = 4.48

Molecular weight: 60.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9ZRU3|A0A1G9ZRU3_9EURY Ribose 1 5-bisphosphate isomerase OS=Haloarchaeobius iranensis OX=996166 GN=SAMN05192554_12215 PE=3 SV=1
AA1 pKa = 7.46TPLPAGWLICISAASVSFRR20 pKa = 11.84AIEE23 pKa = 4.08TACLTKK29 pKa = 10.4RR30 pKa = 11.84KK31 pKa = 8.62RR32 pKa = 11.84TFCTTVLSRR41 pKa = 11.84FTSNVLTARR50 pKa = 11.84PYY52 pKa = 10.73AVRR55 pKa = 11.84GFNRR59 pKa = 11.84AVCDD63 pKa = 3.93SIRR66 pKa = 11.84NRR68 pKa = 11.84RR69 pKa = 11.84DD70 pKa = 2.24WDD72 pKa = 3.81FVIKK76 pKa = 10.45RR77 pKa = 11.84IEE79 pKa = 4.22CFLEE83 pKa = 2.92IWMIIDD89 pKa = 3.7AVFGLLAHH97 pKa = 6.91DD98 pKa = 4.72WYY100 pKa = 10.28RR101 pKa = 11.84TDD103 pKa = 3.77SRR105 pKa = 11.84EE106 pKa = 3.91ASNYY110 pKa = 7.72PQLIDD115 pKa = 3.34VRR117 pKa = 11.84KK118 pKa = 8.84IRR120 pKa = 11.84EE121 pKa = 3.88DD122 pKa = 3.38SRR124 pKa = 11.84VQDD127 pKa = 3.52ARR129 pKa = 11.84IRR131 pKa = 11.84WTITTGHH138 pKa = 6.54FADD141 pKa = 5.14DD142 pKa = 3.84LSLPEE147 pKa = 4.45SSSSNSSSLAASTSYY162 pKa = 10.87AASSSSTTPRR172 pKa = 11.84RR173 pKa = 11.84SSTPCSRR180 pKa = 11.84RR181 pKa = 11.84MSRR184 pKa = 11.84IVHH187 pKa = 6.66RR188 pKa = 11.84STSASLAASPSVIRR202 pKa = 11.84SRR204 pKa = 11.84SS205 pKa = 3.26

Molecular weight: 22.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3852

0

3852

1096490

29

1715

284.7

30.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.121 ± 0.055

0.756 ± 0.014

8.566 ± 0.057

8.302 ± 0.06

3.296 ± 0.025

8.779 ± 0.036

2.022 ± 0.02

3.363 ± 0.031

1.666 ± 0.024

8.9 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.019

2.247 ± 0.023

4.704 ± 0.025

2.542 ± 0.024

6.53 ± 0.041

5.476 ± 0.034

6.559 ± 0.036

9.602 ± 0.051

1.172 ± 0.016

2.665 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski