Brumimicrobium sp. C305

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Crocinitomicaceae; Brumimicrobium; unclassified Brumimicrobium

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2XAX5|A0A2U2XAX5_9FLAO QacE family quaternary ammonium compound efflux SMR transporter OS=Brumimicrobium sp. C305 OX=2100725 GN=DIT68_12170 PE=3 SV=1
MM1 pKa = 7.14KK2 pKa = 10.05TFLRR6 pKa = 11.84SVAVFIAASFTMNASAQIPDD26 pKa = 3.49NGIWPAGVTFTDD38 pKa = 3.78INGTTHH44 pKa = 7.54DD45 pKa = 4.38MDD47 pKa = 5.27ALLDD51 pKa = 4.02AGKK54 pKa = 8.13TVIIDD59 pKa = 5.17AFADD63 pKa = 3.26WCAPCWNYY71 pKa = 10.28HH72 pKa = 5.4QGHH75 pKa = 6.61ALEE78 pKa = 4.71NLYY81 pKa = 8.52TQYY84 pKa = 11.32GPNGTDD90 pKa = 2.78EE91 pKa = 5.02LMVLGIEE98 pKa = 4.42ADD100 pKa = 3.5PGEE103 pKa = 4.55AEE105 pKa = 5.13SNISDD110 pKa = 4.51PNTGMGDD117 pKa = 3.17WTVGISYY124 pKa = 8.93PQANDD129 pKa = 3.42DD130 pKa = 4.83AIAGIINLAYY140 pKa = 9.08YY141 pKa = 6.72PTVIMVCPDD150 pKa = 3.23RR151 pKa = 11.84SVTEE155 pKa = 4.05VGQEE159 pKa = 3.93STANLYY165 pKa = 10.81AGISTCGAPASNSNDD180 pKa = 2.73PRR182 pKa = 11.84LIANASDD189 pKa = 3.68NLFCAGSSANMSVVLQNFSTAPLTSATIEE218 pKa = 4.19VMDD221 pKa = 4.66GATSVATEE229 pKa = 3.64NWTGNLAPYY238 pKa = 10.38DD239 pKa = 3.82VTTIALGSVSPSGSTNYY256 pKa = 9.13TIKK259 pKa = 9.49ITSANDD265 pKa = 3.17DD266 pKa = 3.75TSNDD270 pKa = 3.41QITASVAPAPVLRR283 pKa = 11.84VGDD286 pKa = 3.39QTKK289 pKa = 10.54AVNVVLAGDD298 pKa = 4.15AYY300 pKa = 10.07KK301 pKa = 10.9DD302 pKa = 3.27EE303 pKa = 4.38TGIIFDD309 pKa = 4.67EE310 pKa = 5.29GPLPNEE316 pKa = 4.19SFVTIHH322 pKa = 6.6NDD324 pKa = 2.85AAGGITTPLGFAQVGTLPQGAAPLTVTYY352 pKa = 10.75NAVNEE357 pKa = 4.35GCHH360 pKa = 5.09YY361 pKa = 10.84VVIVDD366 pKa = 4.5SYY368 pKa = 11.85GDD370 pKa = 3.92GINYY374 pKa = 8.47QMPTAEE380 pKa = 5.65LRR382 pKa = 11.84IQGDD386 pKa = 4.08GTSKK390 pKa = 10.44IDD392 pKa = 3.4VNPDD396 pKa = 3.06FGDD399 pKa = 4.79GIAVLFDD406 pKa = 3.27VQFQTEE412 pKa = 4.31VSVNTEE418 pKa = 3.76EE419 pKa = 4.32FASSFKK425 pKa = 10.92VFPNPTNSVVNVEE438 pKa = 5.28FEE440 pKa = 4.31LQNQEE445 pKa = 4.24DD446 pKa = 4.52VTVSIMNTVGQTVAVNNLGTVSGVQATQMDD476 pKa = 4.4VSSLEE481 pKa = 3.79SGMYY485 pKa = 8.54IVKK488 pKa = 10.33VKK490 pKa = 9.12TANGEE495 pKa = 3.85QTKK498 pKa = 10.23RR499 pKa = 11.84ISVIKK504 pKa = 10.54

Molecular weight: 53.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2XGK8|A0A2U2XGK8_9FLAO Phosphohistidine phosphatase SixA OS=Brumimicrobium sp. C305 OX=2100725 GN=DIT68_00845 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.56HH16 pKa = 3.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.11NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.46KK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.95SLTVSSNKK48 pKa = 9.3LAKK51 pKa = 10.16RR52 pKa = 3.58

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3111

0

3111

1076708

22

2970

346.1

39.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.113 ± 0.044

0.792 ± 0.02

5.6 ± 0.038

7.01 ± 0.057

5.277 ± 0.041

6.568 ± 0.051

1.766 ± 0.019

7.852 ± 0.04

7.429 ± 0.075

9.051 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.024

6.164 ± 0.053

3.355 ± 0.03

3.374 ± 0.027

3.468 ± 0.035

6.871 ± 0.048

5.66 ± 0.07

6.261 ± 0.033

1.055 ± 0.016

4.02 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski