Amino acid dipepetide frequency for Brumimicrobium sp. C305

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.862AlaAla: 3.862 ± 0.072	0.581AlaCys: 0.581 ± 0.028	3.419AlaAsp: 3.419 ± 0.075	4.125AlaGlu: 4.125 ± 0.082	3.268AlaPhe: 3.268 ± 0.052	4.112AlaGly: 4.112 ± 0.087	1.096AlaHis: 1.096 ± 0.038	5.029AlaIle: 5.029 ± 0.069	4.464AlaLys: 4.464 ± 0.083	5.867AlaLeu: 5.867 ± 0.084	1.589AlaMet: 1.589 ± 0.044	3.338AlaAsn: 3.338 ± 0.067	1.936AlaPro: 1.936 ± 0.055	2.375AlaGln: 2.375 ± 0.049	1.892AlaArg: 1.892 ± 0.046	3.971AlaSer: 3.971 ± 0.072	3.34AlaThr: 3.34 ± 0.082	3.835AlaVal: 3.835 ± 0.065	0.635AlaTrp: 0.635 ± 0.029	2.394AlaTyr: 2.394 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.505CysAla: 0.505 ± 0.025	0.081CysCys: 0.081 ± 0.009	0.54CysAsp: 0.54 ± 0.039	0.52CysGlu: 0.52 ± 0.028	0.393CysPhe: 0.393 ± 0.021	0.713CysGly: 0.713 ± 0.036	0.165CysHis: 0.165 ± 0.013	0.538CysIle: 0.538 ± 0.028	0.46CysLys: 0.46 ± 0.02	0.619CysLeu: 0.619 ± 0.025	0.133CysMet: 0.133 ± 0.011	0.426CysAsn: 0.426 ± 0.024	0.424CysPro: 0.424 ± 0.037	0.225CysGln: 0.225 ± 0.017	0.224CysArg: 0.224 ± 0.012	0.619CysSer: 0.619 ± 0.027	0.478CysThr: 0.478 ± 0.038	0.441CysVal: 0.441 ± 0.025	0.091CysTrp: 0.091 ± 0.01	0.324CysTyr: 0.324 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.243AspAla: 3.243 ± 0.065	0.492AspCys: 0.492 ± 0.033	2.82AspAsp: 2.82 ± 0.066	4.097AspGlu: 4.097 ± 0.074	3.822AspPhe: 3.822 ± 0.065	3.856AspGly: 3.856 ± 0.064	0.959AspHis: 0.959 ± 0.028	4.297AspIle: 4.297 ± 0.067	3.919AspLys: 3.919 ± 0.069	5.551AspLeu: 5.551 ± 0.084	1.099AspMet: 1.099 ± 0.037	2.992AspAsn: 2.992 ± 0.07	1.806AspPro: 1.806 ± 0.046	1.858AspGln: 1.858 ± 0.045	1.816AspArg: 1.816 ± 0.044	3.526AspSer: 3.526 ± 0.066	2.764AspThr: 2.764 ± 0.056	3.693AspVal: 3.693 ± 0.081	0.708AspTrp: 0.708 ± 0.026	2.679AspTyr: 2.679 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	4.246GluAla: 4.246 ± 0.086	0.429GluCys: 0.429 ± 0.018	3.631GluAsp: 3.631 ± 0.07	5.875GluGlu: 5.875 ± 0.1	3.249GluPhe: 3.249 ± 0.055	4.027GluGly: 4.027 ± 0.066	1.136GluHis: 1.136 ± 0.033	6.036GluIle: 6.036 ± 0.081	6.172GluLys: 6.172 ± 0.114	6.838GluLeu: 6.838 ± 0.114	1.942GluMet: 1.942 ± 0.048	4.978GluAsn: 4.978 ± 0.093	1.452GluPro: 1.452 ± 0.048	2.332GluGln: 2.332 ± 0.054	2.666GluArg: 2.666 ± 0.058	3.711GluSer: 3.711 ± 0.064	3.391GluThr: 3.391 ± 0.056	4.648GluVal: 4.648 ± 0.072	0.739GluTrp: 0.739 ± 0.026	2.603GluTyr: 2.603 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	2.911PheAla: 2.911 ± 0.053	0.406PheCys: 0.406 ± 0.016	3.325PheAsp: 3.325 ± 0.057	3.648PheGlu: 3.648 ± 0.053	2.732PhePhe: 2.732 ± 0.062	3.602PheGly: 3.602 ± 0.06	0.912PheHis: 0.912 ± 0.027	3.99PheIle: 3.99 ± 0.08	3.67PheLys: 3.67 ± 0.068	4.66PheLeu: 4.66 ± 0.088	1.219PheMet: 1.219 ± 0.035	3.239PheAsn: 3.239 ± 0.059	1.807PhePro: 1.807 ± 0.051	1.606PheGln: 1.606 ± 0.045	1.654PheArg: 1.654 ± 0.043	4.316PheSer: 4.316 ± 0.073	3.226PheThr: 3.226 ± 0.069	3.105PheVal: 3.105 ± 0.063	0.56PheTrp: 0.56 ± 0.025	2.185PheTyr: 2.185 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.285GlyAla: 4.285 ± 0.08	0.665GlyCys: 0.665 ± 0.042	3.606GlyAsp: 3.606 ± 0.071	4.183GlyGlu: 4.183 ± 0.072	3.59GlyPhe: 3.59 ± 0.072	4.94GlyGly: 4.94 ± 0.111	1.129GlyHis: 1.129 ± 0.036	5.267GlyIle: 5.267 ± 0.086	4.772GlyLys: 4.772 ± 0.077	5.717GlyLeu: 5.717 ± 0.084	1.73GlyMet: 1.73 ± 0.046	3.926GlyAsn: 3.926 ± 0.101	1.399GlyPro: 1.399 ± 0.043	2.014GlyGln: 2.014 ± 0.043	2.179GlyArg: 2.179 ± 0.046	4.067GlySer: 4.067 ± 0.076	4.173GlyThr: 4.173 ± 0.109	4.727GlyVal: 4.727 ± 0.083	0.682GlyTrp: 0.682 ± 0.024	2.633GlyTyr: 2.633 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	0.959HisAla: 0.959 ± 0.031	0.19HisCys: 0.19 ± 0.013	0.822HisAsp: 0.822 ± 0.026	1.067HisGlu: 1.067 ± 0.032	1.109HisPhe: 1.109 ± 0.033	1.085HisGly: 1.085 ± 0.039	0.47HisHis: 0.47 ± 0.024	1.371HisIle: 1.371 ± 0.036	1.176HisLys: 1.176 ± 0.037	1.823HisLeu: 1.823 ± 0.048	0.28HisMet: 0.28 ± 0.015	0.932HisAsn: 0.932 ± 0.031	0.881HisPro: 0.881 ± 0.024	0.683HisGln: 0.683 ± 0.023	0.697HisArg: 0.697 ± 0.026	1.125HisSer: 1.125 ± 0.033	0.928HisThr: 0.928 ± 0.031	0.99HisVal: 0.99 ± 0.033	0.2HisTrp: 0.2 ± 0.015	0.869HisTyr: 0.869 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.215IleAla: 5.215 ± 0.077	0.592IleCys: 0.592 ± 0.031	5.143IleAsp: 5.143 ± 0.07	5.676IleGlu: 5.676 ± 0.084	3.544IlePhe: 3.544 ± 0.071	5.228IleGly: 5.228 ± 0.072	1.483IleHis: 1.483 ± 0.045	5.745IleIle: 5.745 ± 0.097	5.382IleLys: 5.382 ± 0.081	6.622IleLeu: 6.622 ± 0.098	1.388IleMet: 1.388 ± 0.039	4.758IleAsn: 4.758 ± 0.073	3.066IlePro: 3.066 ± 0.053	2.888IleGln: 2.888 ± 0.053	2.744IleArg: 2.744 ± 0.062	6.019IleSer: 6.019 ± 0.073	4.547IleThr: 4.547 ± 0.12	4.856IleVal: 4.856 ± 0.072	0.694IleTrp: 0.694 ± 0.025	2.931IleTyr: 2.931 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	4.595LysAla: 4.595 ± 0.078	0.372LysCys: 0.372 ± 0.019	4.249LysAsp: 4.249 ± 0.065	6.394LysGlu: 6.394 ± 0.123	2.777LysPhe: 2.777 ± 0.054	4.403LysGly: 4.403 ± 0.071	1.414LysHis: 1.414 ± 0.036	5.828LysIle: 5.828 ± 0.078	6.329LysLys: 6.329 ± 0.105	6.131LysLeu: 6.131 ± 0.089	2.041LysMet: 2.041 ± 0.049	4.815LysAsn: 4.815 ± 0.079	2.128LysPro: 2.128 ± 0.053	2.448LysGln: 2.448 ± 0.06	2.854LysArg: 2.854 ± 0.058	4.884LysSer: 4.884 ± 0.076	4.227LysThr: 4.227 ± 0.073	4.718LysVal: 4.718 ± 0.073	0.805LysTrp: 0.805 ± 0.031	2.874LysTyr: 2.874 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	5.529LeuAla: 5.529 ± 0.09	0.588LeuCys: 0.588 ± 0.024	4.971LeuAsp: 4.971 ± 0.081	5.941LeuGlu: 5.941 ± 0.09	4.989LeuPhe: 4.989 ± 0.096	5.682LeuGly: 5.682 ± 0.081	1.485LeuHis: 1.485 ± 0.04	7.176LeuIle: 7.176 ± 0.104	7.322LeuLys: 7.322 ± 0.1	8.245LeuLeu: 8.245 ± 0.124	2.249LeuMet: 2.249 ± 0.05	5.722LeuAsn: 5.722 ± 0.084	3.366LeuPro: 3.366 ± 0.056	2.744LeuGln: 2.744 ± 0.058	3.247LeuArg: 3.247 ± 0.059	6.907LeuSer: 6.907 ± 0.086	5.185LeuThr: 5.185 ± 0.081	5.285LeuVal: 5.285 ± 0.077	0.809LeuTrp: 0.809 ± 0.028	3.071LeuTyr: 3.071 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.527MetAla: 1.527 ± 0.039	0.134MetCys: 0.134 ± 0.012	1.283MetAsp: 1.283 ± 0.035	1.594MetGlu: 1.594 ± 0.038	0.883MetPhe: 0.883 ± 0.03	1.536MetGly: 1.536 ± 0.039	0.4MetHis: 0.4 ± 0.022	1.689MetIle: 1.689 ± 0.042	2.371MetLys: 2.371 ± 0.043	1.946MetLeu: 1.946 ± 0.05	0.672MetMet: 0.672 ± 0.028	1.565MetAsn: 1.565 ± 0.04	0.747MetPro: 0.747 ± 0.026	0.759MetGln: 0.759 ± 0.028	1.008MetArg: 1.008 ± 0.034	1.525MetSer: 1.525 ± 0.04	1.251MetThr: 1.251 ± 0.04	1.373MetVal: 1.373 ± 0.04	0.175MetTrp: 0.175 ± 0.012	0.693MetTyr: 0.693 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.809AsnAla: 3.809 ± 0.076	0.584AsnCys: 0.584 ± 0.041	3.292AsnAsp: 3.292 ± 0.066	4.1AsnGlu: 4.1 ± 0.092	2.976AsnPhe: 2.976 ± 0.062	4.372AsnGly: 4.372 ± 0.105	1.102AsnHis: 1.102 ± 0.032	4.537AsnIle: 4.537 ± 0.083	4.172AsnLys: 4.172 ± 0.07	5.074AsnLeu: 5.074 ± 0.074	1.251AsnMet: 1.251 ± 0.036	3.918AsnAsn: 3.918 ± 0.105	2.797AsnPro: 2.797 ± 0.055	2.418AsnGln: 2.418 ± 0.052	2.172AsnArg: 2.172 ± 0.052	4.256AsnSer: 4.256 ± 0.097	3.505AsnThr: 3.505 ± 0.077	3.5AsnVal: 3.5 ± 0.067	0.827AsnTrp: 0.827 ± 0.03	2.975AsnTyr: 2.975 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.01ProAla: 2.01 ± 0.061	0.263ProCys: 0.263 ± 0.015	1.913ProAsp: 1.913 ± 0.038	2.66ProGlu: 2.66 ± 0.057	1.891ProPhe: 1.891 ± 0.049	1.971ProGly: 1.971 ± 0.051	0.627ProHis: 0.627 ± 0.025	2.658ProIle: 2.658 ± 0.055	2.354ProLys: 2.354 ± 0.055	2.799ProLeu: 2.799 ± 0.05	0.774ProMet: 0.774 ± 0.025	2.402ProAsn: 2.402 ± 0.057	0.883ProPro: 0.883 ± 0.031	1.047ProGln: 1.047 ± 0.032	0.927ProArg: 0.927 ± 0.03	2.393ProSer: 2.393 ± 0.055	2.105ProThr: 2.105 ± 0.069	2.216ProVal: 2.216 ± 0.052	0.275ProTrp: 0.275 ± 0.016	1.383ProTyr: 1.383 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	1.958GlnAla: 1.958 ± 0.048	0.247GlnCys: 0.247 ± 0.027	1.607GlnAsp: 1.607 ± 0.037	2.571GlnGlu: 2.571 ± 0.063	1.818GlnPhe: 1.818 ± 0.046	1.929GlnGly: 1.929 ± 0.048	0.586GlnHis: 0.586 ± 0.026	2.668GlnIle: 2.668 ± 0.055	2.66GlnLys: 2.66 ± 0.056	3.546GlnLeu: 3.546 ± 0.061	0.854GlnMet: 0.854 ± 0.029	2.153GlnAsn: 2.153 ± 0.055	0.982GlnPro: 0.982 ± 0.027	1.312GlnGln: 1.312 ± 0.042	1.323GlnArg: 1.323 ± 0.036	2.12GlnSer: 2.12 ± 0.049	1.834GlnThr: 1.834 ± 0.045	1.95GlnVal: 1.95 ± 0.042	0.374GlnTrp: 0.374 ± 0.019	1.251GlnTyr: 1.251 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	1.87ArgAla: 1.87 ± 0.046	0.202ArgCys: 0.202 ± 0.014	1.752ArgAsp: 1.752 ± 0.041	2.219ArgGlu: 2.219 ± 0.057	1.951ArgPhe: 1.951 ± 0.043	1.965ArgGly: 1.965 ± 0.041	0.58ArgHis: 0.58 ± 0.025	3.014ArgIle: 3.014 ± 0.058	3.069ArgLys: 3.069 ± 0.067	3.326ArgLeu: 3.326 ± 0.066	0.999ArgMet: 0.999 ± 0.033	2.091ArgAsn: 2.091 ± 0.055	1.054ArgPro: 1.054 ± 0.029	1.1ArgGln: 1.1 ± 0.033	1.432ArgArg: 1.432 ± 0.043	2.103ArgSer: 2.103 ± 0.049	1.815ArgThr: 1.815 ± 0.044	2.236ArgVal: 2.236 ± 0.045	0.384ArgTrp: 0.384 ± 0.021	1.521ArgTyr: 1.521 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.226SerAla: 4.226 ± 0.075	0.717SerCys: 0.717 ± 0.028	3.627SerAsp: 3.627 ± 0.077	4.344SerGlu: 4.344 ± 0.071	4.251SerPhe: 4.251 ± 0.073	4.851SerGly: 4.851 ± 0.091	1.049SerHis: 1.049 ± 0.029	5.633SerIle: 5.633 ± 0.089	4.727SerLys: 4.727 ± 0.075	6.181SerLeu: 6.181 ± 0.078	1.392SerMet: 1.392 ± 0.036	4.123SerAsn: 4.123 ± 0.093	2.434SerPro: 2.434 ± 0.063	2.24SerGln: 2.24 ± 0.043	2.131SerArg: 2.131 ± 0.05	5.04SerSer: 5.04 ± 0.098	4.033SerThr: 4.033 ± 0.082	4.195SerVal: 4.195 ± 0.071	0.735SerTrp: 0.735 ± 0.029	2.783SerTyr: 2.783 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	3.702ThrAla: 3.702 ± 0.085	0.438ThrCys: 0.438 ± 0.043	3.227ThrAsp: 3.227 ± 0.095	3.489ThrGlu: 3.489 ± 0.076	2.947ThrPhe: 2.947 ± 0.049	4.014ThrGly: 4.014 ± 0.089	0.996ThrHis: 0.996 ± 0.031	4.783ThrIle: 4.783 ± 0.095	3.422ThrLys: 3.422 ± 0.065	5.257ThrLeu: 5.257 ± 0.082	1.054ThrMet: 1.054 ± 0.035	3.165ThrAsn: 3.165 ± 0.078	2.495ThrPro: 2.495 ± 0.056	1.9ThrGln: 1.9 ± 0.046	1.605ThrArg: 1.605 ± 0.046	3.935ThrSer: 3.935 ± 0.089	3.423ThrThr: 3.423 ± 0.092	3.843ThrVal: 3.843 ± 0.105	0.652ThrTrp: 0.652 ± 0.035	2.257ThrTyr: 2.257 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	3.756ValAla: 3.756 ± 0.061	0.533ValCys: 0.533 ± 0.027	3.664ValAsp: 3.664 ± 0.057	4.398ValGlu: 4.398 ± 0.068	3.522ValPhe: 3.522 ± 0.071	4.026ValGly: 4.026 ± 0.079	1.039ValHis: 1.039 ± 0.034	4.809ValIle: 4.809 ± 0.077	4.335ValLys: 4.335 ± 0.078	5.76ValLeu: 5.76 ± 0.083	1.415ValMet: 1.415 ± 0.036	3.91ValAsn: 3.91 ± 0.07	2.082ValPro: 2.082 ± 0.046	1.965ValGln: 1.965 ± 0.046	2.132ValArg: 2.132 ± 0.052	4.432ValSer: 4.432 ± 0.091	3.466ValThr: 3.466 ± 0.085	4.227ValVal: 4.227 ± 0.074	0.606ValTrp: 0.606 ± 0.025	2.535ValTyr: 2.535 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.556TrpAla: 0.556 ± 0.024	0.093TrpCys: 0.093 ± 0.01	0.657TrpAsp: 0.657 ± 0.025	0.678TrpGlu: 0.678 ± 0.032	0.553TrpPhe: 0.553 ± 0.021	0.709TrpGly: 0.709 ± 0.032	0.196TrpHis: 0.196 ± 0.014	0.824TrpIle: 0.824 ± 0.032	0.844TrpLys: 0.844 ± 0.03	0.86TrpLeu: 0.86 ± 0.029	0.328TrpMet: 0.328 ± 0.015	0.742TrpAsn: 0.742 ± 0.029	0.214TrpPro: 0.214 ± 0.014	0.34TrpGln: 0.34 ± 0.018	0.424TrpArg: 0.424 ± 0.02	0.799TrpSer: 0.799 ± 0.028	0.597TrpThr: 0.597 ± 0.032	0.623TrpVal: 0.623 ± 0.025	0.128TrpTrp: 0.128 ± 0.012	0.387TrpTyr: 0.387 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.364TyrAla: 2.364 ± 0.048	0.313TyrCys: 0.313 ± 0.016	2.453TyrAsp: 2.453 ± 0.058	2.523TyrGlu: 2.523 ± 0.053	2.508TyrPhe: 2.508 ± 0.056	2.664TyrGly: 2.664 ± 0.062	0.832TyrHis: 0.832 ± 0.029	2.63TyrIle: 2.63 ± 0.059	2.666TyrLys: 2.666 ± 0.063	3.694TyrLeu: 3.694 ± 0.079	0.719TyrMet: 0.719 ± 0.025	2.441TyrAsn: 2.441 ± 0.056	1.599TyrPro: 1.599 ± 0.04	1.489TyrGln: 1.489 ± 0.043	1.554TyrArg: 1.554 ± 0.043	2.961TyrSer: 2.961 ± 0.056	2.316TyrThr: 2.316 ± 0.059	2.152TyrVal: 2.152 ± 0.043	0.474TyrTrp: 0.474 ± 0.023	1.85TyrTyr: 1.85 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3111 proteins (1076708 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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