Streptococcus phage O1205 (Streptococcus bacteriophage phi-O1205)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O34054|O34054_BPO12 ORF23 OS=Streptococcus phage O1205 OX=85154 PE=4 SV=1
MM1 pKa = 7.49TKK3 pKa = 10.0TIEE6 pKa = 4.24LLNSFKK12 pKa = 10.4PDD14 pKa = 3.0SYY16 pKa = 11.85DD17 pKa = 3.39FGNARR22 pKa = 11.84YY23 pKa = 9.8GSLEE27 pKa = 3.87EE28 pKa = 4.25LKK30 pKa = 10.86EE31 pKa = 3.73LLVYY35 pKa = 10.39KK36 pKa = 10.47RR37 pKa = 11.84IVKK40 pKa = 8.88WDD42 pKa = 3.41KK43 pKa = 11.42DD44 pKa = 3.99HH45 pKa = 7.89IEE47 pKa = 4.65LEE49 pKa = 4.74DD50 pKa = 3.69GTKK53 pKa = 8.97VTIEE57 pKa = 3.92MSEE60 pKa = 4.09NDD62 pKa = 3.39CCAYY66 pKa = 10.54AGGSFKK72 pKa = 10.63DD73 pKa = 3.62VKK75 pKa = 10.68LDD77 pKa = 5.23AIITDD82 pKa = 3.63IKK84 pKa = 10.54IGEE87 pKa = 4.23QEE89 pKa = 4.29TVGCEE94 pKa = 3.99DD95 pKa = 4.4GSSEE99 pKa = 4.55SYY101 pKa = 9.13NTVTIYY107 pKa = 10.9HH108 pKa = 5.81NQNPVALAEE117 pKa = 4.37CEE119 pKa = 4.47ANDD122 pKa = 4.05GNGGFYY128 pKa = 10.73YY129 pKa = 10.53SVGSLVIGDD138 pKa = 2.96IHH140 pKa = 6.9FPVVEE145 pKa = 4.09AA146 pKa = 4.75

Molecular weight: 16.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O34071|O34071_BPO12 ORF40 OS=Streptococcus phage O1205 OX=85154 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.49EE18 pKa = 3.53MKK20 pKa = 9.92LYY22 pKa = 10.32QKK24 pKa = 10.55AYY26 pKa = 9.84KK27 pKa = 10.03KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.36IYY38 pKa = 9.68QLAYY42 pKa = 10.87LNRR45 pKa = 11.84LANATTKK52 pKa = 10.62DD53 pKa = 3.03GKK55 pKa = 10.64KK56 pKa = 10.46YY57 pKa = 10.49YY58 pKa = 10.19FEE60 pKa = 5.48KK61 pKa = 10.83FDD63 pKa = 3.95DD64 pKa = 4.49FYY66 pKa = 11.56NAKK69 pKa = 9.54EE70 pKa = 3.83RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.84VLGEE78 pKa = 4.35KK79 pKa = 8.61ITNSKK84 pKa = 10.18LLEE87 pKa = 3.98RR88 pKa = 11.84ARR90 pKa = 11.84NNLNYY95 pKa = 10.06KK96 pKa = 9.85KK97 pKa = 10.46EE98 pKa = 3.94RR99 pKa = 11.84GLLDD103 pKa = 3.22GRR105 pKa = 4.46

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13345

42

1517

234.1

26.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.119 ± 0.928

0.674 ± 0.127

6.309 ± 0.491

6.594 ± 0.689

3.972 ± 0.199

7.044 ± 0.474

1.281 ± 0.165

6.939 ± 0.39

7.936 ± 0.551

7.606 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.257

5.77 ± 0.376

2.862 ± 0.22

3.694 ± 0.271

4.121 ± 0.316

6.924 ± 0.607

6.639 ± 0.464

6.692 ± 0.215

1.394 ± 0.176

4.159 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski