Anaerostipes sp. 992a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerostipes; unclassified Anaerostipes

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2529 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9K2Z4|A0A1Q9K2Z4_9FIRM Uncharacterized protein OS=Anaerostipes sp. 992a OX=1261637 GN=BHF69_09890 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.2KK3 pKa = 10.36KK4 pKa = 10.46KK5 pKa = 10.76NLGITNLNGVTDD17 pKa = 5.67DD18 pKa = 4.38EE19 pKa = 4.85DD20 pKa = 4.3TTCGITDD27 pKa = 4.44LNCVDD32 pKa = 4.05GQDD35 pKa = 3.38TGSYY39 pKa = 10.43SITDD43 pKa = 4.01LNAVDD48 pKa = 6.06DD49 pKa = 5.19KK50 pKa = 11.53DD51 pKa = 4.09TTCDD55 pKa = 3.5ITDD58 pKa = 4.4LNCNEE63 pKa = 4.08

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9K3S2|A0A1Q9K3S2_9FIRM Uncharacterized protein OS=Anaerostipes sp. 992a OX=1261637 GN=BHF69_11310 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84NKK41 pKa = 10.44LSAA44 pKa = 3.84

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2529

0

2529

795659

44

2101

314.6

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.951 ± 0.052

1.527 ± 0.019

5.476 ± 0.04

7.852 ± 0.058

4.023 ± 0.036

7.065 ± 0.043

1.881 ± 0.024

7.859 ± 0.045

7.667 ± 0.05

8.676 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.26 ± 0.024

4.122 ± 0.027

3.176 ± 0.029

3.475 ± 0.031

4.283 ± 0.038

5.595 ± 0.039

5.227 ± 0.049

6.792 ± 0.044

0.845 ± 0.015

4.247 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski