Gordonia phage Yvonnetastic
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 198 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142K9G1|A0A142K9G1_9CAUD Uncharacterized protein OS=Gordonia phage Yvonnetastic OX=1821566 GN=200 PE=4 SV=1
MM1 pKa = 7.81 SNTTCDD7 pKa = 3.71 HH8 pKa = 6.89 DD9 pKa = 3.83 WQLVEE14 pKa = 4.64 SGYY17 pKa = 10.31 SRR19 pKa = 11.84 YY20 pKa = 9.54 WSVSIDD26 pKa = 3.66 DD27 pKa = 5.39 DD28 pKa = 4.28 GTIHH32 pKa = 7.46 AGEE35 pKa = 4.69 DD36 pKa = 2.94 WMTDD40 pKa = 3.11 DD41 pKa = 4.26 GDD43 pKa = 4.14 GVRR46 pKa = 11.84 LVCLNCQTTRR56 pKa = 11.84 KK57 pKa = 10.06 DD58 pKa = 3.53 DD59 pKa = 5.61 LNIQWFF65 pKa = 3.61
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.656
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.579
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.342
Thurlkill 3.745
EMBOSS 3.91
Sillero 4.024
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A142K976|A0A142K976_9CAUD Uncharacterized protein OS=Gordonia phage Yvonnetastic OX=1821566 GN=115 PE=4 SV=1
MM1 pKa = 7.94 IVMSEE6 pKa = 4.56 HH7 pKa = 5.57 NQDD10 pKa = 2.81 WLPRR14 pKa = 11.84 FHH16 pKa = 7.27 SGYY19 pKa = 10.05 RR20 pKa = 11.84 IEE22 pKa = 5.06 PSGCHH27 pKa = 4.7 VWQKK31 pKa = 9.25 ATNNRR36 pKa = 11.84 GYY38 pKa = 11.37 GVMYY42 pKa = 10.78 LNGKK46 pKa = 8.33 LQLAHH51 pKa = 6.73 RR52 pKa = 11.84 VAFFSRR58 pKa = 11.84 HH59 pKa = 4.39 GRR61 pKa = 11.84 WPQRR65 pKa = 11.84 GLVIDD70 pKa = 4.48 HH71 pKa = 6.74 LCEE74 pKa = 4.19 NKK76 pKa = 10.07 PCVNPDD82 pKa = 3.21 HH83 pKa = 6.8 LRR85 pKa = 11.84 EE86 pKa = 4.01 IEE88 pKa = 3.51 NWRR91 pKa = 11.84 NVRR94 pKa = 11.84 RR95 pKa = 11.84 PRR97 pKa = 11.84 EE98 pKa = 3.98 VGSTDD103 pKa = 3.05 QEE105 pKa = 3.89 RR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 EE109 pKa = 3.8 QWRR112 pKa = 11.84 IRR114 pKa = 11.84 QARR117 pKa = 11.84 CRR119 pKa = 11.84 GSYY122 pKa = 10.31 SSTYY126 pKa = 7.5 EE127 pKa = 3.71 AKK129 pKa = 10.48 RR130 pKa = 3.41
Molecular weight: 15.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.531
IPC_protein 10.452
Toseland 10.262
ProMoST 10.321
Dawson 10.467
Bjellqvist 10.262
Wikipedia 10.73
Rodwell 10.526
Grimsley 10.555
Solomon 10.57
Lehninger 10.526
Nozaki 10.335
DTASelect 10.248
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.145
IPC_peptide 10.57
IPC2_peptide 9.575
IPC2.peptide.svr19 8.541
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
198
0
198
31266
25
2765
157.9
17.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.504 ± 0.449
1.452 ± 0.177
6.848 ± 0.19
7.168 ± 0.237
2.869 ± 0.108
8.006 ± 0.252
2.444 ± 0.164
4.977 ± 0.142
4.602 ± 0.186
7.753 ± 0.158
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.498 ± 0.092
3.429 ± 0.107
4.897 ± 0.171
3.716 ± 0.185
6.774 ± 0.18
5.795 ± 0.162
5.575 ± 0.221
6.736 ± 0.192
2.476 ± 0.113
3.483 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here