Chimpanzee faeces associated microphage 2
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A186YBX9|A0A186YBX9_9VIRU Minor CP 1 OS=Chimpanzee faeces associated microphage 2 OX=1676182 PE=4 SV=1
MM1 pKa = 7.98 EE2 pKa = 4.94 YY3 pKa = 8.97 RR4 pKa = 11.84 TIMNRR9 pKa = 11.84 QRR11 pKa = 11.84 ICSNSGSRR19 pKa = 11.84 FAPTYY24 pKa = 9.58 SLKK27 pKa = 9.44 ITDD30 pKa = 5.23 DD31 pKa = 3.73 GNKK34 pKa = 10.48 DD35 pKa = 3.34 LVEE38 pKa = 4.14 TGKK41 pKa = 10.48 VDD43 pKa = 3.48 VYY45 pKa = 11.66 GKK47 pKa = 9.39 IQTYY51 pKa = 9.9 KK52 pKa = 9.58 EE53 pKa = 3.98 SCDD56 pKa = 2.84 IHH58 pKa = 7.99 VILEE62 pKa = 4.11 RR63 pKa = 11.84 FVNGDD68 pKa = 3.28 EE69 pKa = 4.33 SALNAGTPSFGDD81 pKa = 3.3 FTEE84 pKa = 4.51 YY85 pKa = 8.01 PTTYY89 pKa = 11.25 AEE91 pKa = 4.17 MLQRR95 pKa = 11.84 THH97 pKa = 6.0 EE98 pKa = 4.2 AEE100 pKa = 4.16 EE101 pKa = 4.29 MFMQLPLEE109 pKa = 4.21 VRR111 pKa = 11.84 AEE113 pKa = 4.32 FNHH116 pKa = 6.2 SPGEE120 pKa = 4.05 FFTSIGSEE128 pKa = 4.08 RR129 pKa = 11.84 FNGVYY134 pKa = 10.33 DD135 pKa = 3.71 KK136 pKa = 11.25 YY137 pKa = 11.27 FKK139 pKa = 10.71 IEE141 pKa = 4.05 DD142 pKa = 3.72 SVASVEE148 pKa = 4.12 PVTPVVQKK156 pKa = 10.78 EE157 pKa = 4.21 VVTDD161 pKa = 3.66 VEE163 pKa = 4.31 KK164 pKa = 11.39 SEE166 pKa = 4.1
Molecular weight: 18.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.651
IPC2_protein 4.685
IPC_protein 4.584
Toseland 4.457
ProMoST 4.622
Dawson 4.52
Bjellqvist 4.698
Wikipedia 4.368
Rodwell 4.431
Grimsley 4.368
Solomon 4.52
Lehninger 4.469
Nozaki 4.635
DTASelect 4.749
Thurlkill 4.444
EMBOSS 4.393
Sillero 4.698
Patrickios 3.757
IPC_peptide 4.533
IPC2_peptide 4.698
IPC2.peptide.svr19 4.632
Protein with the highest isoelectric point:
>tr|A0A186YBP6|A0A186YBP6_9VIRU Minor CP 2 OS=Chimpanzee faeces associated microphage 2 OX=1676182 PE=4 SV=1
MM1 pKa = 7.49 RR2 pKa = 11.84 LIYY5 pKa = 10.21 RR6 pKa = 11.84 LLASMPKK13 pKa = 9.89 LHH15 pKa = 7.2 SNRR18 pKa = 11.84 QLLVRR23 pKa = 11.84 RR24 pKa = 11.84 LPCMQLIVLRR34 pKa = 11.84 RR35 pKa = 11.84 LPCMVLIRR43 pKa = 11.84 LLLLLCMVLTRR54 pKa = 11.84 LLKK57 pKa = 9.1 LTRR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 LTGKK66 pKa = 10.13 SFTLIILIRR75 pKa = 11.84 LVLLCITRR83 pKa = 11.84 VKK85 pKa = 10.62 SQEE88 pKa = 3.91 NLKK91 pKa = 10.61 LLYY94 pKa = 9.27 STLSIFLIGILAEE107 pKa = 4.29 TKK109 pKa = 10.4 KK110 pKa = 9.83 MRR112 pKa = 11.84 PLMRR116 pKa = 11.84 AHH118 pKa = 7.31
Molecular weight: 13.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.171
IPC2_protein 10.379
IPC_protein 11.754
Toseland 11.915
ProMoST 12.398
Dawson 11.915
Bjellqvist 11.901
Wikipedia 12.384
Rodwell 11.696
Grimsley 11.959
Solomon 12.398
Lehninger 12.31
Nozaki 11.915
DTASelect 11.901
Thurlkill 11.915
EMBOSS 12.413
Sillero 11.915
Patrickios 11.418
IPC_peptide 12.398
IPC2_peptide 11.389
IPC2.peptide.svr19 9.276
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1446
118
580
289.2
32.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.607 ± 1.946
0.899 ± 0.45
6.155 ± 0.798
5.256 ± 1.402
3.734 ± 0.508
5.187 ± 0.471
1.867 ± 0.288
5.256 ± 0.529
5.394 ± 1.342
8.368 ± 2.211
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.697 ± 0.435
6.708 ± 1.276
4.08 ± 0.793
3.942 ± 0.939
5.878 ± 1.083
8.299 ± 0.855
6.362 ± 0.669
5.809 ± 1.275
1.037 ± 0.258
5.463 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here