Hyaloscypha bicolor E

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Hyaloscyphaceae; Hyaloscypha; Hyaloscypha hepaticicola/Rhizoscyphus ericae species complex; Hyaloscypha bicolor

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2J6T643|A0A2J6T643_9HELO Uncharacterized protein OS=Hyaloscypha bicolor E OX=1095630 GN=K444DRAFT_614246 PE=4 SV=1
MM1 pKa = 7.64LGLLLAISVLSSTCLAKK18 pKa = 10.38AVPPTPSQAPKK29 pKa = 9.68PFLNITEE36 pKa = 4.83LVACVPPSIRR46 pKa = 11.84VTVVSPGTGGKK57 pKa = 7.87NTSTQLSVPLFTDD70 pKa = 3.68CTYY73 pKa = 11.33QKK75 pKa = 6.9TTPVTPGQNNAPSSAISSSADD96 pKa = 2.99YY97 pKa = 11.15SQFVDD102 pKa = 3.51ANGNALDD109 pKa = 4.12KK110 pKa = 11.5NSVNTFLGFFGDD122 pKa = 4.65SVDD125 pKa = 4.11LDD127 pKa = 4.04VILEE131 pKa = 4.17VTACAITDD139 pKa = 3.63TNNNVDD145 pKa = 4.37TIQSEE150 pKa = 4.41NEE152 pKa = 3.79ALNDD156 pKa = 3.82GNSVSILNYY165 pKa = 9.69QCITEE170 pKa = 5.3LIDD173 pKa = 3.34SS174 pKa = 4.33

Molecular weight: 18.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2J6SNA7|A0A2J6SNA7_9HELO Uncharacterized protein OS=Hyaloscypha bicolor E OX=1095630 GN=K444DRAFT_637431 PE=4 SV=1
MM1 pKa = 7.52PPSHH5 pKa = 6.59SAHH8 pKa = 4.59STKK11 pKa = 10.07RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84NGLSEE21 pKa = 4.72LIRR24 pKa = 11.84RR25 pKa = 11.84KK26 pKa = 9.88RR27 pKa = 11.84RR28 pKa = 11.84ARR30 pKa = 11.84SLLRR34 pKa = 11.84NPRR37 pKa = 11.84KK38 pKa = 8.82PRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84NLNPLRR50 pKa = 11.84ISRR53 pKa = 11.84GGLSEE58 pKa = 3.58LRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 9.56RR64 pKa = 11.84RR65 pKa = 11.84ARR67 pKa = 11.84SLLRR71 pKa = 11.84NSS73 pKa = 3.56

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18610

6

18616

7838171

49

7281

421.0

46.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.056 ± 0.014

1.407 ± 0.009

5.308 ± 0.014

6.23 ± 0.017

3.945 ± 0.012

6.931 ± 0.016

2.333 ± 0.009

5.325 ± 0.016

5.078 ± 0.015

9.172 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.006

3.832 ± 0.01

5.802 ± 0.021

3.879 ± 0.012

5.889 ± 0.016

8.296 ± 0.021

5.976 ± 0.013

5.991 ± 0.011

1.576 ± 0.007

2.803 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski