Naumannella halotolerans
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R7J8X6|A0A4R7J8X6_9ACTN DNA-binding NarL/FixJ family response regulator OS=Naumannella halotolerans OX=993414 GN=CLV29_1600 PE=4 SV=1
MM1 pKa = 7.05 VQGTSQAVPEE11 pKa = 4.5 RR12 pKa = 11.84 NHH14 pKa = 4.14 VTNRR18 pKa = 11.84 ARR20 pKa = 11.84 STTRR24 pKa = 11.84 IAAGLAALSLGLAACSGGGSSDD46 pKa = 3.75 GEE48 pKa = 4.18 GDD50 pKa = 3.29 GGEE53 pKa = 4.44 DD54 pKa = 3.38 LTVTSSISQDD64 pKa = 2.94 VDD66 pKa = 3.29 TLLPMDD72 pKa = 5.17 ANVGDD77 pKa = 4.67 NIAVLDD83 pKa = 4.21 VIYY86 pKa = 10.69 DD87 pKa = 3.48 GLVRR91 pKa = 11.84 YY92 pKa = 9.8 DD93 pKa = 3.84 PEE95 pKa = 4.16 TTEE98 pKa = 4.31 PYY100 pKa = 10.01 PYY102 pKa = 10.09 LAEE105 pKa = 5.02 EE106 pKa = 4.21 ITTTDD111 pKa = 3.29 NQVWTIKK118 pKa = 10.0 IKK120 pKa = 10.74 PGTTFHH126 pKa = 6.7 NGEE129 pKa = 4.29 PVDD132 pKa = 3.91 AEE134 pKa = 3.99 AFARR138 pKa = 11.84 AWNYY142 pKa = 9.76 AAYY145 pKa = 9.89 GPNAMGNNYY154 pKa = 9.21 FFEE157 pKa = 6.25 RR158 pKa = 11.84 IEE160 pKa = 5.34 GYY162 pKa = 11.04 DD163 pKa = 3.68 DD164 pKa = 3.37 MQGEE168 pKa = 4.48 YY169 pKa = 10.83 EE170 pKa = 4.16 EE171 pKa = 6.46 ADD173 pKa = 3.86 DD174 pKa = 4.14 GTVTVDD180 pKa = 3.46 EE181 pKa = 5.05 EE182 pKa = 4.13 PAADD186 pKa = 3.88 EE187 pKa = 4.26 LSGLEE192 pKa = 4.09 VVDD195 pKa = 4.21 PQTLQVTLNAPFVGFDD211 pKa = 3.21 TMLGYY216 pKa = 9.18 TGFFPIAQACLDD228 pKa = 4.89 DD229 pKa = 5.88 IEE231 pKa = 6.12 ACTTKK236 pKa = 10.61 PIGNGPFQVEE246 pKa = 4.57 EE247 pKa = 4.22 WSQGQSLTATKK258 pKa = 10.48 YY259 pKa = 10.87 ADD261 pKa = 3.44 YY262 pKa = 10.55 TGAEE266 pKa = 4.19 TPNYY270 pKa = 10.42 DD271 pKa = 3.37 RR272 pKa = 11.84 IEE274 pKa = 4.02 WVEE277 pKa = 3.79 YY278 pKa = 10.69 AGGSEE283 pKa = 4.1 WPDD286 pKa = 3.68 FQAGLMDD293 pKa = 4.78 LAEE296 pKa = 4.55 APSANFTQAYY306 pKa = 8.85 DD307 pKa = 3.62 DD308 pKa = 4.48 PALQRR313 pKa = 11.84 VEE315 pKa = 4.52 RR316 pKa = 11.84 PTAALTYY323 pKa = 10.54 LSFPLYY329 pKa = 10.58 DD330 pKa = 4.64 DD331 pKa = 5.74 GPWTDD336 pKa = 2.72 IEE338 pKa = 4.27 FRR340 pKa = 11.84 KK341 pKa = 9.78 ALSMAIDD348 pKa = 3.81 RR349 pKa = 11.84 QAVIDD354 pKa = 4.22 AVLPGQRR361 pKa = 11.84 VPADD365 pKa = 3.2 SWVMPDD371 pKa = 3.71 GVPGGVAGTCGEE383 pKa = 4.1 ACTFNPEE390 pKa = 3.62 AAKK393 pKa = 10.42 AALEE397 pKa = 4.16 AAGGWPEE404 pKa = 4.27 GEE406 pKa = 4.32 TMTIHH411 pKa = 6.89 LGVDD415 pKa = 3.22 EE416 pKa = 4.65 TEE418 pKa = 4.11 QEE420 pKa = 4.05 LFQAIGDD427 pKa = 4.04 QITLNLGIPYY437 pKa = 9.91 EE438 pKa = 4.5 LDD440 pKa = 3.21 PTPDD444 pKa = 3.13 FFDD447 pKa = 3.47 RR448 pKa = 11.84 RR449 pKa = 11.84 SNRR452 pKa = 11.84 DD453 pKa = 2.69 FSGPFRR459 pKa = 11.84 SNWFPDD465 pKa = 3.37 YY466 pKa = 10.55 PLNEE470 pKa = 4.4 NYY472 pKa = 10.41 LGPVYY477 pKa = 10.72 ASDD480 pKa = 4.26 DD481 pKa = 3.83 AEE483 pKa = 4.1 EE484 pKa = 4.87 GNTNFGYY491 pKa = 10.98 YY492 pKa = 10.24 NEE494 pKa = 5.12 DD495 pKa = 3.45 FNNAIADD502 pKa = 3.86 GDD504 pKa = 4.15 SAATLDD510 pKa = 3.97 EE511 pKa = 4.83 AVTNYY516 pKa = 10.19 QEE518 pKa = 4.31 AEE520 pKa = 4.18 RR521 pKa = 11.84 ILAEE525 pKa = 4.65 DD526 pKa = 5.04 FPTAPLFYY534 pKa = 9.44 TNVVLFHH541 pKa = 6.93 SDD543 pKa = 2.79 RR544 pKa = 11.84 VSNVVIDD551 pKa = 4.28 PFSGRR556 pKa = 11.84 AKK558 pKa = 9.63 MRR560 pKa = 11.84 LLEE563 pKa = 4.15 VTGG566 pKa = 3.81
Molecular weight: 61.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.91
Patrickios 1.443
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A4R7J1X9|A0A4R7J1X9_9ACTN DNA-binding IclR family transcriptional regulator OS=Naumannella halotolerans OX=993414 GN=CLV29_2584 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 THH17 pKa = 6.18 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 10.05 GRR40 pKa = 11.84 TKK42 pKa = 10.74 LAGG45 pKa = 3.42
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3108
0
3108
1020358
27
2777
328.3
35.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.528 ± 0.05
0.694 ± 0.013
6.236 ± 0.037
5.99 ± 0.04
2.885 ± 0.026
9.035 ± 0.041
1.969 ± 0.017
4.403 ± 0.03
2.016 ± 0.029
10.225 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.892 ± 0.014
2.078 ± 0.021
5.736 ± 0.036
3.309 ± 0.022
7.295 ± 0.051
5.719 ± 0.034
6.017 ± 0.038
8.549 ± 0.04
1.52 ± 0.02
1.907 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here