Flavobacterium sp. LB1R34

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A553E8K0|A0A553E8K0_9FLAO Uncharacterized protein OS=Flavobacterium sp. LB1R34 OX=2594428 GN=FNW21_04560 PE=4 SV=1
MM1 pKa = 7.2VYY3 pKa = 10.24KK4 pKa = 10.58FRR6 pKa = 11.84VILDD10 pKa = 3.42AEE12 pKa = 4.04EE13 pKa = 5.63DD14 pKa = 3.42IFRR17 pKa = 11.84DD18 pKa = 3.48IAILEE23 pKa = 4.6DD24 pKa = 3.77DD25 pKa = 4.13TLEE28 pKa = 4.56DD29 pKa = 3.14LHH31 pKa = 7.78NAIFNSFGFDD41 pKa = 3.19GMEE44 pKa = 3.67VASFYY49 pKa = 11.0TCDD52 pKa = 3.51EE53 pKa = 4.1TWNQEE58 pKa = 3.85DD59 pKa = 5.24EE60 pKa = 4.6IPMFDD65 pKa = 3.3TGDD68 pKa = 3.27IPGEE72 pKa = 4.03QKK74 pKa = 9.82TMSDD78 pKa = 4.07YY79 pKa = 10.96ILSEE83 pKa = 4.0ILDD86 pKa = 3.75KK87 pKa = 11.5QNTKK91 pKa = 10.09IIYY94 pKa = 9.9VYY96 pKa = 10.91DD97 pKa = 5.09FINMWTFLVEE107 pKa = 3.97LAAVEE112 pKa = 4.15DD113 pKa = 4.11QVAGNMYY120 pKa = 9.91PEE122 pKa = 5.08TIFSHH127 pKa = 7.86GEE129 pKa = 3.86MPDD132 pKa = 3.02EE133 pKa = 4.28AMEE136 pKa = 4.54KK137 pKa = 10.39NFEE140 pKa = 4.28ADD142 pKa = 3.14MHH144 pKa = 6.64DD145 pKa = 4.5DD146 pKa = 3.19IYY148 pKa = 11.63GEE150 pKa = 4.38FEE152 pKa = 5.39DD153 pKa = 6.28DD154 pKa = 5.21LDD156 pKa = 5.18EE157 pKa = 6.67DD158 pKa = 4.46DD159 pKa = 6.43LDD161 pKa = 4.11MFSGDD166 pKa = 4.99DD167 pKa = 3.5SFEE170 pKa = 4.49DD171 pKa = 4.77FGFEE175 pKa = 4.53EE176 pKa = 4.36NWNN179 pKa = 3.64

Molecular weight: 20.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553DQI8|A0A553DQI8_9FLAO Alpha-amylase OS=Flavobacterium sp. LB1R34 OX=2594428 GN=FNW21_15630 PE=4 SV=1
MM1 pKa = 7.58PPKK4 pKa = 10.22KK5 pKa = 9.96RR6 pKa = 11.84AAKK9 pKa = 10.27KK10 pKa = 9.08NGSSFFVVCVPSLHH24 pKa = 5.61TVAPFVRR31 pKa = 11.84RR32 pKa = 11.84GYY34 pKa = 10.83CVFFSTIFFLFLSVEE49 pKa = 4.0NFQLKK54 pKa = 10.29KK55 pKa = 10.59NSPKK59 pKa = 10.49KK60 pKa = 9.57STATCVLAVAQPPAINLNRR79 pKa = 11.84NAKK82 pKa = 10.26ADD84 pKa = 3.83LNYY87 pKa = 10.45LFRR90 pKa = 11.84YY91 pKa = 9.91RR92 pKa = 11.84FTSSVAVTRR101 pKa = 11.84SHH103 pKa = 7.1PSSPEE108 pKa = 3.31KK109 pKa = 10.62GLPSLRR115 pKa = 11.84WLSVTATPPLVQQKK129 pKa = 10.26KK130 pKa = 10.12AFYY133 pKa = 10.06RR134 pKa = 11.84CC135 pKa = 3.4

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3141

0

3141

1086059

36

3959

345.8

38.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.84 ± 0.045

0.784 ± 0.013

5.169 ± 0.033

5.993 ± 0.055

5.325 ± 0.04

6.33 ± 0.047

1.656 ± 0.019

8.073 ± 0.045

7.911 ± 0.062

9.229 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.023

6.362 ± 0.043

3.388 ± 0.023

3.618 ± 0.023

3.015 ± 0.026

6.345 ± 0.042

6.489 ± 0.079

6.203 ± 0.035

1.054 ± 0.017

4.058 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski