Amino acid dipepetide frequency for Flavobacterium sp. LB1R34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.814AlaAla: 4.814 ± 0.083	0.557AlaCys: 0.557 ± 0.027	3.237AlaAsp: 3.237 ± 0.062	3.546AlaGlu: 3.546 ± 0.065	3.535AlaPhe: 3.535 ± 0.064	4.419AlaGly: 4.419 ± 0.086	1.061AlaHis: 1.061 ± 0.03	6.283AlaIle: 6.283 ± 0.084	5.064AlaLys: 5.064 ± 0.078	6.504AlaLeu: 6.504 ± 0.093	1.578AlaMet: 1.578 ± 0.041	4.011AlaAsn: 4.011 ± 0.075	2.045AlaPro: 2.045 ± 0.051	2.7AlaGln: 2.7 ± 0.057	1.876AlaArg: 1.876 ± 0.048	4.267AlaSer: 4.267 ± 0.08	5.312AlaThr: 5.312 ± 0.146	4.595AlaVal: 4.595 ± 0.068	0.626AlaTrp: 0.626 ± 0.026	2.375AlaTyr: 2.375 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.454CysAla: 0.454 ± 0.025	0.109CysCys: 0.109 ± 0.012	0.445CysAsp: 0.445 ± 0.022	0.443CysGlu: 0.443 ± 0.023	0.474CysPhe: 0.474 ± 0.021	0.601CysGly: 0.601 ± 0.026	0.177CysHis: 0.177 ± 0.014	0.627CysIle: 0.627 ± 0.031	0.475CysLys: 0.475 ± 0.021	0.683CysLeu: 0.683 ± 0.027	0.147CysMet: 0.147 ± 0.01	0.46CysAsn: 0.46 ± 0.02	0.342CysPro: 0.342 ± 0.023	0.251CysGln: 0.251 ± 0.017	0.176CysArg: 0.176 ± 0.012	0.6CysSer: 0.6 ± 0.031	0.468CysThr: 0.468 ± 0.023	0.478CysVal: 0.478 ± 0.023	0.076CysTrp: 0.076 ± 0.008	0.358CysTyr: 0.358 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.899AspAla: 3.899 ± 0.067	0.451AspCys: 0.451 ± 0.021	2.344AspAsp: 2.344 ± 0.058	3.158AspGlu: 3.158 ± 0.066	3.745AspPhe: 3.745 ± 0.056	3.59AspGly: 3.59 ± 0.083	0.735AspHis: 0.735 ± 0.028	3.757AspIle: 3.757 ± 0.061	4.158AspLys: 4.158 ± 0.076	5.116AspLeu: 5.116 ± 0.076	0.972AspMet: 0.972 ± 0.032	2.882AspAsn: 2.882 ± 0.053	1.538AspPro: 1.538 ± 0.039	1.413AspGln: 1.413 ± 0.04	1.586AspArg: 1.586 ± 0.041	2.848AspSer: 2.848 ± 0.048	2.719AspThr: 2.719 ± 0.055	3.292AspVal: 3.292 ± 0.061	0.722AspTrp: 0.722 ± 0.026	2.762AspTyr: 2.762 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.039GluAla: 4.039 ± 0.083	0.314GluCys: 0.314 ± 0.019	2.747GluAsp: 2.747 ± 0.061	3.817GluGlu: 3.817 ± 0.081	2.738GluPhe: 2.738 ± 0.049	3.127GluGly: 3.127 ± 0.058	0.89GluHis: 0.89 ± 0.031	5.681GluIle: 5.681 ± 0.081	5.887GluLys: 5.887 ± 0.096	5.665GluLeu: 5.665 ± 0.104	1.575GluMet: 1.575 ± 0.043	4.665GluAsn: 4.665 ± 0.077	1.37GluPro: 1.37 ± 0.036	2.184GluGln: 2.184 ± 0.05	2.027GluArg: 2.027 ± 0.051	3.17GluSer: 3.17 ± 0.058	3.64GluThr: 3.64 ± 0.063	3.62GluVal: 3.62 ± 0.064	0.56GluTrp: 0.56 ± 0.027	2.217GluTyr: 2.217 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.336PheAla: 3.336 ± 0.059	0.466PheCys: 0.466 ± 0.02	3.382PheAsp: 3.382 ± 0.06	3.538PheGlu: 3.538 ± 0.068	2.799PhePhe: 2.799 ± 0.058	3.769PheGly: 3.769 ± 0.061	0.84PheHis: 0.84 ± 0.032	3.86PheIle: 3.86 ± 0.075	3.785PheLys: 3.785 ± 0.073	4.838PheLeu: 4.838 ± 0.08	1.119PheMet: 1.119 ± 0.034	3.075PheAsn: 3.075 ± 0.056	1.819PhePro: 1.819 ± 0.044	1.757PheGln: 1.757 ± 0.043	1.548PheArg: 1.548 ± 0.04	4.003PheSer: 4.003 ± 0.061	3.211PheThr: 3.211 ± 0.07	3.236PheVal: 3.236 ± 0.06	0.643PheTrp: 0.643 ± 0.025	2.231PheTyr: 2.231 ± 0.046	0.001PheXaa: 0.001 ± 0.001
Gly	4.196GlyAla: 4.196 ± 0.08	0.591GlyCys: 0.591 ± 0.03	3.071GlyAsp: 3.071 ± 0.065	3.033GlyGlu: 3.033 ± 0.053	3.809GlyPhe: 3.809 ± 0.053	4.422GlyGly: 4.422 ± 0.085	1.054GlyHis: 1.054 ± 0.037	5.652GlyIle: 5.652 ± 0.067	5.119GlyLys: 5.119 ± 0.078	5.678GlyLeu: 5.678 ± 0.079	1.576GlyMet: 1.576 ± 0.039	3.847GlyAsn: 3.847 ± 0.073	1.146GlyPro: 1.146 ± 0.032	1.986GlyGln: 1.986 ± 0.043	1.837GlyArg: 1.837 ± 0.05	4.045GlySer: 4.045 ± 0.082	4.588GlyThr: 4.588 ± 0.134	4.146GlyVal: 4.146 ± 0.072	0.743GlyTrp: 0.743 ± 0.03	2.755GlyTyr: 2.755 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	0.936HisAla: 0.936 ± 0.029	0.183HisCys: 0.183 ± 0.013	0.82HisAsp: 0.82 ± 0.029	0.887HisGlu: 0.887 ± 0.03	1.255HisPhe: 1.255 ± 0.039	0.935HisGly: 0.935 ± 0.029	0.47HisHis: 0.47 ± 0.025	1.257HisIle: 1.257 ± 0.036	1.114HisLys: 1.114 ± 0.03	1.749HisLeu: 1.749 ± 0.04	0.265HisMet: 0.265 ± 0.017	0.905HisAsn: 0.905 ± 0.037	0.843HisPro: 0.843 ± 0.032	0.756HisGln: 0.756 ± 0.029	0.547HisArg: 0.547 ± 0.026	0.968HisSer: 0.968 ± 0.03	0.852HisThr: 0.852 ± 0.025	0.807HisVal: 0.807 ± 0.027	0.2HisTrp: 0.2 ± 0.014	0.81HisTyr: 0.81 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.496IleAla: 6.496 ± 0.091	0.677IleCys: 0.677 ± 0.027	4.742IleAsp: 4.742 ± 0.062	5.179IleGlu: 5.179 ± 0.084	3.378IlePhe: 3.378 ± 0.065	5.139IleGly: 5.139 ± 0.076	1.387IleHis: 1.387 ± 0.042	6.088IleIle: 6.088 ± 0.092	6.04IleLys: 6.04 ± 0.098	7.167IleLeu: 7.167 ± 0.099	1.357IleMet: 1.357 ± 0.035	4.765IleAsn: 4.765 ± 0.079	3.492IlePro: 3.492 ± 0.058	3.042IleGln: 3.042 ± 0.063	2.462IleArg: 2.462 ± 0.045	5.358IleSer: 5.358 ± 0.084	5.395IleThr: 5.395 ± 0.089	5.004IleVal: 5.004 ± 0.083	0.708IleTrp: 0.708 ± 0.028	2.853IleTyr: 2.853 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	5.073LysAla: 5.073 ± 0.078	0.384LysCys: 0.384 ± 0.022	3.963LysAsp: 3.963 ± 0.077	5.507LysGlu: 5.507 ± 0.103	3.049LysPhe: 3.049 ± 0.051	4.592LysGly: 4.592 ± 0.067	1.142LysHis: 1.142 ± 0.036	7.314LysIle: 7.314 ± 0.079	7.067LysLys: 7.067 ± 0.092	6.369LysLeu: 6.369 ± 0.082	2.291LysMet: 2.291 ± 0.05	5.816LysAsn: 5.816 ± 0.093	2.485LysPro: 2.485 ± 0.046	2.78LysGln: 2.78 ± 0.059	2.443LysArg: 2.443 ± 0.053	4.749LysSer: 4.749 ± 0.066	5.38LysThr: 5.38 ± 0.072	4.716LysVal: 4.716 ± 0.074	0.821LysTrp: 0.821 ± 0.032	3.166LysTyr: 3.166 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	6.197LeuAla: 6.197 ± 0.083	0.735LeuCys: 0.735 ± 0.026	5.13LeuAsp: 5.13 ± 0.083	5.829LeuGlu: 5.829 ± 0.09	5.236LeuPhe: 5.236 ± 0.086	5.847LeuGly: 5.847 ± 0.092	1.631LeuHis: 1.631 ± 0.04	6.664LeuIle: 6.664 ± 0.106	7.662LeuLys: 7.662 ± 0.096	9.047LeuLeu: 9.047 ± 0.143	1.957LeuMet: 1.957 ± 0.048	5.588LeuAsn: 5.588 ± 0.074	3.459LeuPro: 3.459 ± 0.057	3.543LeuGln: 3.543 ± 0.075	2.756LeuArg: 2.756 ± 0.059	6.2LeuSer: 6.2 ± 0.067	5.337LeuThr: 5.337 ± 0.096	5.503LeuVal: 5.503 ± 0.077	0.788LeuTrp: 0.788 ± 0.03	3.179LeuTyr: 3.179 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	1.579MetAla: 1.579 ± 0.041	0.151MetCys: 0.151 ± 0.012	1.069MetAsp: 1.069 ± 0.031	1.282MetGlu: 1.282 ± 0.035	0.773MetPhe: 0.773 ± 0.027	1.359MetGly: 1.359 ± 0.04	0.378MetHis: 0.378 ± 0.022	1.531MetIle: 1.531 ± 0.039	2.232MetLys: 2.232 ± 0.042	1.865MetLeu: 1.865 ± 0.041	0.61MetMet: 0.61 ± 0.027	1.429MetAsn: 1.429 ± 0.04	0.805MetPro: 0.805 ± 0.027	0.877MetGln: 0.877 ± 0.031	0.779MetArg: 0.779 ± 0.026	1.389MetSer: 1.389 ± 0.039	1.261MetThr: 1.261 ± 0.04	1.336MetVal: 1.336 ± 0.038	0.151MetTrp: 0.151 ± 0.013	0.721MetTyr: 0.721 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	4.347AsnAla: 4.347 ± 0.069	0.547AsnCys: 0.547 ± 0.027	3.126AsnAsp: 3.126 ± 0.055	3.759AsnGlu: 3.759 ± 0.065	3.209AsnPhe: 3.209 ± 0.065	4.369AsnGly: 4.369 ± 0.086	1.145AsnHis: 1.145 ± 0.033	4.331AsnIle: 4.331 ± 0.057	4.557AsnLys: 4.557 ± 0.078	5.506AsnLeu: 5.506 ± 0.077	1.178AsnMet: 1.178 ± 0.029	3.898AsnAsn: 3.898 ± 0.085	3.037AsnPro: 3.037 ± 0.056	2.7AsnGln: 2.7 ± 0.054	2.029AsnArg: 2.029 ± 0.052	4.143AsnSer: 4.143 ± 0.077	4.267AsnThr: 4.267 ± 0.088	3.457AsnVal: 3.457 ± 0.064	0.856AsnTrp: 0.856 ± 0.028	3.156AsnTyr: 3.156 ± 0.061	0.0AsnXaa: 0.0 ± 0.0
Pro	2.18ProAla: 2.18 ± 0.051	0.217ProCys: 0.217 ± 0.014	1.87ProAsp: 1.87 ± 0.041	2.563ProGlu: 2.563 ± 0.061	1.994ProPhe: 1.994 ± 0.036	1.619ProGly: 1.619 ± 0.047	0.527ProHis: 0.527 ± 0.026	2.876ProIle: 2.876 ± 0.059	2.651ProLys: 2.651 ± 0.055	2.944ProLeu: 2.944 ± 0.064	0.727ProMet: 0.727 ± 0.025	2.439ProAsn: 2.439 ± 0.059	0.744ProPro: 0.744 ± 0.035	1.11ProGln: 1.11 ± 0.038	0.8ProArg: 0.8 ± 0.028	2.129ProSer: 2.129 ± 0.045	2.417ProThr: 2.417 ± 0.063	2.357ProVal: 2.357 ± 0.052	0.343ProTrp: 0.343 ± 0.018	1.377ProTyr: 1.377 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.134GlnAla: 2.134 ± 0.047	0.212GlnCys: 0.212 ± 0.018	1.678GlnAsp: 1.678 ± 0.038	2.536GlnGlu: 2.536 ± 0.054	1.823GlnPhe: 1.823 ± 0.039	1.759GlnGly: 1.759 ± 0.038	0.583GlnHis: 0.583 ± 0.023	2.817GlnIle: 2.817 ± 0.05	3.477GlnLys: 3.477 ± 0.064	3.656GlnLeu: 3.656 ± 0.058	0.866GlnMet: 0.866 ± 0.027	2.808GlnAsn: 2.808 ± 0.059	1.128GlnPro: 1.128 ± 0.035	1.476GlnGln: 1.476 ± 0.041	1.113GlnArg: 1.113 ± 0.035	2.154GlnSer: 2.154 ± 0.054	2.166GlnThr: 2.166 ± 0.049	2.012GlnVal: 2.012 ± 0.047	0.403GlnTrp: 0.403 ± 0.019	1.383GlnTyr: 1.383 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	1.905ArgAla: 1.905 ± 0.044	0.2ArgCys: 0.2 ± 0.015	1.531ArgAsp: 1.531 ± 0.04	1.849ArgGlu: 1.849 ± 0.053	1.744ArgPhe: 1.744 ± 0.047	1.633ArgGly: 1.633 ± 0.048	0.46ArgHis: 0.46 ± 0.021	2.772ArgIle: 2.772 ± 0.052	2.546ArgLys: 2.546 ± 0.057	2.884ArgLeu: 2.884 ± 0.053	0.803ArgMet: 0.803 ± 0.028	2.013ArgAsn: 2.013 ± 0.047	0.926ArgPro: 0.926 ± 0.031	0.943ArgGln: 0.943 ± 0.029	1.023ArgArg: 1.023 ± 0.035	1.569ArgSer: 1.569 ± 0.042	1.837ArgThr: 1.837 ± 0.038	1.855ArgVal: 1.855 ± 0.045	0.356ArgTrp: 0.356 ± 0.019	1.298ArgTyr: 1.298 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.707SerAla: 3.707 ± 0.074	0.665SerCys: 0.665 ± 0.027	3.218SerAsp: 3.218 ± 0.064	3.556SerGlu: 3.556 ± 0.06	3.823SerPhe: 3.823 ± 0.073	4.773SerGly: 4.773 ± 0.102	1.126SerHis: 1.126 ± 0.045	5.05SerIle: 5.05 ± 0.087	4.899SerLys: 4.899 ± 0.063	5.659SerLeu: 5.659 ± 0.087	1.25SerMet: 1.25 ± 0.036	3.97SerAsn: 3.97 ± 0.076	2.134SerPro: 2.134 ± 0.06	2.292SerGln: 2.292 ± 0.05	1.939SerArg: 1.939 ± 0.052	4.231SerSer: 4.231 ± 0.101	3.691SerThr: 3.691 ± 0.074	4.015SerVal: 4.015 ± 0.087	0.721SerTrp: 0.721 ± 0.027	2.726SerTyr: 2.726 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	4.933ThrAla: 4.933 ± 0.133	0.374ThrCys: 0.374 ± 0.025	3.284ThrAsp: 3.284 ± 0.06	3.313ThrGlu: 3.313 ± 0.061	3.312ThrPhe: 3.312 ± 0.054	4.336ThrGly: 4.336 ± 0.104	1.028ThrHis: 1.028 ± 0.029	5.919ThrIle: 5.919 ± 0.094	4.385ThrLys: 4.385 ± 0.065	5.751ThrLeu: 5.751 ± 0.087	1.052ThrMet: 1.052 ± 0.034	4.048ThrAsn: 4.048 ± 0.09	2.791ThrPro: 2.791 ± 0.065	2.336ThrGln: 2.336 ± 0.049	1.604ThrArg: 1.604 ± 0.039	3.922ThrSer: 3.922 ± 0.116	5.07ThrThr: 5.07 ± 0.18	4.323ThrVal: 4.323 ± 0.101	0.611ThrTrp: 0.611 ± 0.029	2.497ThrTyr: 2.497 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	4.944ValAla: 4.944 ± 0.071	0.521ValCys: 0.521 ± 0.024	3.176ValAsp: 3.176 ± 0.058	3.248ValGlu: 3.248 ± 0.062	3.51ValPhe: 3.51 ± 0.06	3.71ValGly: 3.71 ± 0.067	0.951ValHis: 0.951 ± 0.029	4.815ValIle: 4.815 ± 0.071	4.214ValLys: 4.214 ± 0.055	6.389ValLeu: 6.389 ± 0.097	1.266ValMet: 1.266 ± 0.043	3.48ValAsn: 3.48 ± 0.067	2.144ValPro: 2.144 ± 0.041	1.954ValGln: 1.954 ± 0.046	1.864ValArg: 1.864 ± 0.047	4.241ValSer: 4.241 ± 0.073	4.139ValThr: 4.139 ± 0.107	4.475ValVal: 4.475 ± 0.084	0.591ValTrp: 0.591 ± 0.023	2.396ValTyr: 2.396 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.653TrpAla: 0.653 ± 0.03	0.1TrpCys: 0.1 ± 0.008	0.571TrpAsp: 0.571 ± 0.024	0.633TrpGlu: 0.633 ± 0.027	0.573TrpPhe: 0.573 ± 0.025	0.677TrpGly: 0.677 ± 0.03	0.219TrpHis: 0.219 ± 0.014	0.769TrpIle: 0.769 ± 0.024	0.801TrpLys: 0.801 ± 0.031	0.97TrpLeu: 0.97 ± 0.033	0.306TrpMet: 0.306 ± 0.016	0.82TrpAsn: 0.82 ± 0.029	0.205TrpPro: 0.205 ± 0.016	0.447TrpGln: 0.447 ± 0.021	0.347TrpArg: 0.347 ± 0.019	0.715TrpSer: 0.715 ± 0.027	0.561TrpThr: 0.561 ± 0.027	0.603TrpVal: 0.603 ± 0.025	0.142TrpTrp: 0.142 ± 0.013	0.432TrpTyr: 0.432 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.582TyrAla: 2.582 ± 0.048	0.39TyrCys: 0.39 ± 0.02	2.283TyrAsp: 2.283 ± 0.061	2.259TyrGlu: 2.259 ± 0.046	2.474TyrPhe: 2.474 ± 0.063	2.621TyrGly: 2.621 ± 0.054	0.757TyrHis: 0.757 ± 0.026	2.667TyrIle: 2.667 ± 0.046	2.973TyrLys: 2.973 ± 0.061	3.847TyrLeu: 3.847 ± 0.069	0.681TyrMet: 0.681 ± 0.023	2.697TyrAsn: 2.697 ± 0.072	1.43TyrPro: 1.43 ± 0.035	1.634TyrGln: 1.634 ± 0.049	1.393TyrArg: 1.393 ± 0.045	2.747TyrSer: 2.747 ± 0.059	2.579TyrThr: 2.579 ± 0.061	2.195TyrVal: 2.195 ± 0.048	0.483TyrTrp: 0.483 ± 0.022	1.892TyrTyr: 1.892 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3141 proteins (1086059 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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