Salmonella phage IKe (Bacteriophage IKe)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Lineavirus; Salmonella virus IKe

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03667|G4P_BPIKE Virion export protein OS=Salmonella phage IKe OX=10867 GN=IV PE=3 SV=1
MM1 pKa = 7.15KK2 pKa = 10.05RR3 pKa = 11.84KK4 pKa = 9.92IIAISLFLYY13 pKa = 10.26IPLSNADD20 pKa = 3.16NWEE23 pKa = 4.73SITKK27 pKa = 10.09SYY29 pKa = 9.41YY30 pKa = 9.4TGFAISKK37 pKa = 8.02TVEE40 pKa = 4.13SKK42 pKa = 11.22DD43 pKa = 3.5KK44 pKa = 10.88DD45 pKa = 3.6GKK47 pKa = 9.15PVRR50 pKa = 11.84KK51 pKa = 9.06EE52 pKa = 4.03VITQADD58 pKa = 4.19LTTACNDD65 pKa = 3.48AKK67 pKa = 11.1ASAQNVFNQIKK78 pKa = 8.71LTLSGTWPNSQFRR91 pKa = 11.84LVTGDD96 pKa = 3.27TCVYY100 pKa = 10.11NGSPGEE106 pKa = 4.16KK107 pKa = 8.83TEE109 pKa = 3.93SWSIRR114 pKa = 11.84AQVEE118 pKa = 3.63GDD120 pKa = 3.29IQRR123 pKa = 11.84SVPDD127 pKa = 4.24EE128 pKa = 4.42EE129 pKa = 5.3PSEE132 pKa = 4.06QTPEE136 pKa = 4.36EE137 pKa = 3.99ICEE140 pKa = 4.15AKK142 pKa = 10.56PPIDD146 pKa = 3.35GVFNNVFKK154 pKa = 11.02GDD156 pKa = 3.35EE157 pKa = 3.98GGFYY161 pKa = 10.31INYY164 pKa = 9.6NGCEE168 pKa = 3.98YY169 pKa = 10.28EE170 pKa = 4.12ATGVTVCQNDD180 pKa = 3.77GTVCSSSAWKK190 pKa = 7.96PTGYY194 pKa = 10.11VPEE197 pKa = 4.74SGEE200 pKa = 4.13PSSSPLKK207 pKa = 11.08DD208 pKa = 3.02GDD210 pKa = 3.73TGGTGEE216 pKa = 4.86GGSDD220 pKa = 2.97TGGDD224 pKa = 3.31TGGGDD229 pKa = 3.38TGGGSTGGDD238 pKa = 3.1TGGSSGGGSSGGGSSGGSTGKK259 pKa = 10.44SLTKK263 pKa = 10.41EE264 pKa = 3.95DD265 pKa = 3.36VTAAIHH271 pKa = 5.43VASPSIGDD279 pKa = 3.69AVKK282 pKa = 10.86DD283 pKa = 4.01SLTEE287 pKa = 5.58DD288 pKa = 3.1NDD290 pKa = 3.84QYY292 pKa = 12.07DD293 pKa = 4.0NQKK296 pKa = 10.74KK297 pKa = 10.41ADD299 pKa = 3.92EE300 pKa = 4.18QSAKK304 pKa = 10.72ASASVSDD311 pKa = 5.04AISDD315 pKa = 3.64GMRR318 pKa = 11.84GVGNFVDD325 pKa = 4.54DD326 pKa = 4.6FGGEE330 pKa = 3.92SSQYY334 pKa = 8.8GTGNSEE340 pKa = 3.81MDD342 pKa = 3.99LSVSLAKK349 pKa = 10.52GQLGIDD355 pKa = 3.73RR356 pKa = 11.84EE357 pKa = 4.7GHH359 pKa = 4.86GSAWEE364 pKa = 4.1SFLNDD369 pKa = 3.12GALRR373 pKa = 11.84PSIPTGHH380 pKa = 6.79GCTNFVMYY388 pKa = 10.14QGSVYY393 pKa = 10.25QIEE396 pKa = 4.31IGCDD400 pKa = 2.93KK401 pKa = 11.26LNDD404 pKa = 3.72IKK406 pKa = 11.3SVLSWVMYY414 pKa = 10.54CLTFWYY420 pKa = 10.02VFQSVTSLLRR430 pKa = 11.84KK431 pKa = 10.08GEE433 pKa = 3.98QQ434 pKa = 2.97

Molecular weight: 45.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03658|G1P_BPIKE Gene 1 protein OS=Salmonella phage IKe OX=10867 GN=I PE=3 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84VLSTVLAAKK11 pKa = 10.51NKK13 pKa = 9.02IALGAATMLVSAGSFAAEE31 pKa = 3.81PNAATNYY38 pKa = 8.56ATEE41 pKa = 4.64AMDD44 pKa = 4.09SLKK47 pKa = 9.34TQAIDD52 pKa = 5.42LISQTWPVVTTVVVAGLVIRR72 pKa = 11.84LFKK75 pKa = 10.7KK76 pKa = 10.83FSSKK80 pKa = 10.85AVV82 pKa = 3.23

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

2

11

2253

32

437

204.8

22.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.057 ± 0.591

1.198 ± 0.338

6.17 ± 0.464

3.551 ± 0.815

4.749 ± 0.583

8.123 ± 1.182

1.332 ± 0.33

6.303 ± 0.96

5.992 ± 0.608

8.3 ± 0.858

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.349

5.593 ± 0.82

3.24 ± 0.282

3.95 ± 0.388

3.595 ± 0.412

9.498 ± 0.626

5.859 ± 0.455

8.522 ± 0.856

0.976 ± 0.189

3.684 ± 0.628
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski