Mastigocoleus testarum BC008

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Hapalosiphonaceae; Mastigocoleus; Mastigocoleus testarum

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0V7ZPP3|A0A0V7ZPP3_9CYAN Uncharacterized protein OS=Mastigocoleus testarum BC008 OX=371196 GN=BC008_25540 PE=4 SV=1
MM1 pKa = 7.57SNNAYY6 pKa = 8.63STASSVDD13 pKa = 3.28EE14 pKa = 4.22FQEE17 pKa = 4.44RR18 pKa = 11.84ISARR22 pKa = 11.84WDD24 pKa = 3.09EE25 pKa = 5.16GYY27 pKa = 11.09DD28 pKa = 3.69LVDD31 pKa = 3.14IEE33 pKa = 4.53YY34 pKa = 10.4TDD36 pKa = 5.27GIWFGVFQDD45 pKa = 4.18LPGGNAYY52 pKa = 8.6STANSIGEE60 pKa = 3.99FQEE63 pKa = 4.37KK64 pKa = 9.81IKK66 pKa = 11.0ARR68 pKa = 11.84WDD70 pKa = 3.41EE71 pKa = 4.67KK72 pKa = 10.5YY73 pKa = 11.05DD74 pKa = 3.75LVNVEE79 pKa = 4.65HH80 pKa = 7.31VDD82 pKa = 4.49GIWFGIFQEE91 pKa = 4.62DD92 pKa = 4.15FGANAYY98 pKa = 7.94STASSVDD105 pKa = 3.28EE106 pKa = 4.22FQEE109 pKa = 4.44RR110 pKa = 11.84ISARR114 pKa = 11.84WDD116 pKa = 3.09EE117 pKa = 5.16GYY119 pKa = 11.09DD120 pKa = 3.69LVDD123 pKa = 3.14IEE125 pKa = 4.53YY126 pKa = 10.4TDD128 pKa = 5.27GIWFGVFQDD137 pKa = 4.18LPGGNAYY144 pKa = 8.6STANSIGEE152 pKa = 3.99FQEE155 pKa = 4.37KK156 pKa = 9.81IKK158 pKa = 11.0ARR160 pKa = 11.84WDD162 pKa = 3.41EE163 pKa = 4.67KK164 pKa = 10.5YY165 pKa = 11.05DD166 pKa = 3.75LVNVEE171 pKa = 4.65HH172 pKa = 7.31VDD174 pKa = 4.49GIWFGIFQDD183 pKa = 4.23DD184 pKa = 3.94SSITSAYY191 pKa = 7.79HH192 pKa = 4.85TASTFDD198 pKa = 3.68EE199 pKa = 5.37LIEE202 pKa = 4.46SSQTLWDD209 pKa = 3.62KK210 pKa = 11.02QYY212 pKa = 11.09EE213 pKa = 4.31LVDD216 pKa = 3.27VEE218 pKa = 4.42YY219 pKa = 11.26ADD221 pKa = 5.54GIWFGTFEE229 pKa = 4.34KK230 pKa = 10.46EE231 pKa = 4.48IYY233 pKa = 9.93TPTLNDD239 pKa = 3.32YY240 pKa = 9.88VNQMSQYY247 pKa = 10.55NDD249 pKa = 3.53LLFSQSMALDD259 pKa = 4.06AVNMAIDD266 pKa = 3.45NSIIFF271 pKa = 4.06

Molecular weight: 31.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V7ZH09|A0A0V7ZH09_9CYAN Cytochrome P450 OS=Mastigocoleus testarum BC008 OX=371196 GN=BC008_14830 PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84KK3 pKa = 8.88NRR5 pKa = 11.84VWKK8 pKa = 9.86KK9 pKa = 9.49HH10 pKa = 4.86GIKK13 pKa = 9.9IVFGTVCVVGLFVSRR28 pKa = 11.84DD29 pKa = 3.66DD30 pKa = 3.5IANNIAMTNRR40 pKa = 11.84VKK42 pKa = 11.25AEE44 pKa = 3.79VSASNDD50 pKa = 2.97RR51 pKa = 11.84LSRR54 pKa = 11.84LEE56 pKa = 3.83RR57 pKa = 11.84QRR59 pKa = 11.84ADD61 pKa = 3.39EE62 pKa = 4.05KK63 pKa = 11.21RR64 pKa = 11.84EE65 pKa = 3.97AKK67 pKa = 9.97IAEE70 pKa = 3.92QRR72 pKa = 11.84YY73 pKa = 7.47EE74 pKa = 3.68KK75 pKa = 10.45CLPVVGKK82 pKa = 8.14VRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84GRR88 pKa = 11.84HH89 pKa = 4.06YY90 pKa = 10.68FVGLKK95 pKa = 9.65KK96 pKa = 10.59DD97 pKa = 3.34QLITDD102 pKa = 5.36RR103 pKa = 11.84ISGAPLVRR111 pKa = 11.84GTVVCDD117 pKa = 3.41AFGVTGVLNEE127 pKa = 4.23EE128 pKa = 4.36GRR130 pKa = 11.84VSAVAFTGNRR140 pKa = 11.84DD141 pKa = 3.07LVARR145 pKa = 11.84RR146 pKa = 11.84TKK148 pKa = 10.35RR149 pKa = 11.84FRR151 pKa = 11.84RR152 pKa = 11.84QQYY155 pKa = 9.73SMPVVGDD162 pKa = 3.16VSYY165 pKa = 11.71KK166 pKa = 10.63NGGSNEE172 pKa = 3.96SRR174 pKa = 3.46

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6353

0

6353

2258355

29

4794

355.5

39.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.167 ± 0.033

0.978 ± 0.01

4.971 ± 0.027

6.559 ± 0.032

4.161 ± 0.021

6.499 ± 0.038

1.734 ± 0.014

7.221 ± 0.027

5.868 ± 0.034

10.673 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.015

5.103 ± 0.031

4.31 ± 0.022

4.969 ± 0.026

4.931 ± 0.024

6.903 ± 0.032

5.472 ± 0.025

6.316 ± 0.025

1.373 ± 0.013

3.08 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski