Amino acid dipepetide frequency for Mastigocoleus testarum BC008

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.91AlaAla: 5.91 ± 0.067	0.764AlaCys: 0.764 ± 0.018	3.576AlaAsp: 3.576 ± 0.048	4.455AlaGlu: 4.455 ± 0.053	2.802AlaPhe: 2.802 ± 0.037	4.631AlaGly: 4.631 ± 0.06	1.114AlaHis: 1.114 ± 0.032	6.135AlaIle: 6.135 ± 0.062	4.263AlaLys: 4.263 ± 0.049	7.516AlaLeu: 7.516 ± 0.09	1.362AlaMet: 1.362 ± 0.03	3.544AlaAsn: 3.544 ± 0.07	2.419AlaPro: 2.419 ± 0.035	3.474AlaGln: 3.474 ± 0.048	3.255AlaArg: 3.255 ± 0.049	4.616AlaSer: 4.616 ± 0.058	3.995AlaThr: 3.995 ± 0.053	4.792AlaVal: 4.792 ± 0.055	0.924AlaTrp: 0.924 ± 0.022	2.126AlaTyr: 2.126 ± 0.034	0.002AlaXaa: 0.002 ± 0.001
Cys	0.598CysAla: 0.598 ± 0.017	0.153CysCys: 0.153 ± 0.009	0.557CysAsp: 0.557 ± 0.018	0.582CysGlu: 0.582 ± 0.019	0.444CysPhe: 0.444 ± 0.014	0.751CysGly: 0.751 ± 0.022	0.259CysHis: 0.259 ± 0.012	0.625CysIle: 0.625 ± 0.018	0.455CysLys: 0.455 ± 0.014	1.111CysLeu: 1.111 ± 0.025	0.142CysMet: 0.142 ± 0.008	0.434CysAsn: 0.434 ± 0.018	0.481CysPro: 0.481 ± 0.015	0.517CysGln: 0.517 ± 0.017	0.505CysArg: 0.505 ± 0.017	0.66CysSer: 0.66 ± 0.021	0.439CysThr: 0.439 ± 0.014	0.551CysVal: 0.551 ± 0.017	0.149CysTrp: 0.149 ± 0.009	0.368CysTyr: 0.368 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.228AspAla: 3.228 ± 0.039	0.539AspCys: 0.539 ± 0.015	2.429AspAsp: 2.429 ± 0.047	2.995AspGlu: 2.995 ± 0.044	2.535AspPhe: 2.535 ± 0.04	3.504AspGly: 3.504 ± 0.061	0.537AspHis: 0.537 ± 0.017	4.037AspIle: 4.037 ± 0.05	2.942AspLys: 2.942 ± 0.044	5.737AspLeu: 5.737 ± 0.066	0.766AspMet: 0.766 ± 0.021	2.536AspAsn: 2.536 ± 0.038	2.22AspPro: 2.22 ± 0.039	1.165AspGln: 1.165 ± 0.025	3.093AspArg: 3.093 ± 0.042	3.329AspSer: 3.329 ± 0.048	2.556AspThr: 2.556 ± 0.046	2.929AspVal: 2.929 ± 0.043	0.822AspTrp: 0.822 ± 0.021	1.811AspTyr: 1.811 ± 0.034	0.003AspXaa: 0.003 ± 0.001
Glu	4.898GluAla: 4.898 ± 0.054	0.53GluCys: 0.53 ± 0.017	3.082GluAsp: 3.082 ± 0.041	4.738GluGlu: 4.738 ± 0.062	2.63GluPhe: 2.63 ± 0.035	3.302GluGly: 3.302 ± 0.051	1.03GluHis: 1.03 ± 0.025	5.469GluIle: 5.469 ± 0.059	4.56GluLys: 4.56 ± 0.055	7.087GluLeu: 7.087 ± 0.079	1.211GluMet: 1.211 ± 0.025	3.537GluAsn: 3.537 ± 0.048	2.175GluPro: 2.175 ± 0.035	3.461GluGln: 3.461 ± 0.046	3.298GluArg: 3.298 ± 0.041	3.807GluSer: 3.807 ± 0.051	3.433GluThr: 3.433 ± 0.044	4.458GluVal: 4.458 ± 0.046	0.822GluTrp: 0.822 ± 0.022	2.058GluTyr: 2.058 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.126PheAla: 3.126 ± 0.041	0.545PheCys: 0.545 ± 0.017	2.345PheAsp: 2.345 ± 0.036	2.491PheGlu: 2.491 ± 0.041	1.842PhePhe: 1.842 ± 0.033	3.03PheGly: 3.03 ± 0.044	0.741PheHis: 0.741 ± 0.018	2.77PheIle: 2.77 ± 0.045	1.966PheLys: 1.966 ± 0.031	4.179PheLeu: 4.179 ± 0.056	0.683PheMet: 0.683 ± 0.018	2.002PheAsn: 2.002 ± 0.034	1.839PhePro: 1.839 ± 0.032	1.828PheGln: 1.828 ± 0.032	1.823PheArg: 1.823 ± 0.031	3.349PheSer: 3.349 ± 0.051	2.387PheThr: 2.387 ± 0.037	2.543PheVal: 2.543 ± 0.037	0.695PheTrp: 0.695 ± 0.019	1.422PheTyr: 1.422 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	4.334GlyAla: 4.334 ± 0.066	0.718GlyCys: 0.718 ± 0.017	3.348GlyAsp: 3.348 ± 0.058	4.283GlyGlu: 4.283 ± 0.045	2.999GlyPhe: 2.999 ± 0.038	4.716GlyGly: 4.716 ± 0.087	1.219GlyHis: 1.219 ± 0.031	5.135GlyIle: 5.135 ± 0.053	4.731GlyLys: 4.731 ± 0.068	6.389GlyLeu: 6.389 ± 0.061	1.362GlyMet: 1.362 ± 0.029	3.536GlyAsn: 3.536 ± 0.076	1.235GlyPro: 1.235 ± 0.027	2.342GlyGln: 2.342 ± 0.039	3.05GlyArg: 3.05 ± 0.043	4.2GlySer: 4.2 ± 0.053	3.609GlyThr: 3.609 ± 0.05	4.497GlyVal: 4.497 ± 0.055	1.008GlyTrp: 1.008 ± 0.022	2.278GlyTyr: 2.278 ± 0.035	0.002GlyXaa: 0.002 ± 0.001
His	0.924HisAla: 0.924 ± 0.024	0.22HisCys: 0.22 ± 0.009	0.67HisAsp: 0.67 ± 0.019	0.906HisGlu: 0.906 ± 0.023	0.817HisPhe: 0.817 ± 0.021	1.035HisGly: 1.035 ± 0.024	0.541HisHis: 0.541 ± 0.02	1.107HisIle: 1.107 ± 0.024	0.965HisLys: 0.965 ± 0.023	2.032HisLeu: 2.032 ± 0.034	0.25HisMet: 0.25 ± 0.009	0.86HisAsn: 0.86 ± 0.023	1.125HisPro: 1.125 ± 0.024	1.06HisGln: 1.06 ± 0.025	0.984HisArg: 0.984 ± 0.023	1.203HisSer: 1.203 ± 0.028	0.899HisThr: 0.899 ± 0.024	0.796HisVal: 0.796 ± 0.02	0.311HisTrp: 0.311 ± 0.013	0.635HisTyr: 0.635 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	6.478IleAla: 6.478 ± 0.064	0.825IleCys: 0.825 ± 0.02	3.979IleAsp: 3.979 ± 0.045	4.728IleGlu: 4.728 ± 0.05	3.176IlePhe: 3.176 ± 0.043	4.52IleGly: 4.52 ± 0.054	1.287IleHis: 1.287 ± 0.026	4.565IleIle: 4.565 ± 0.057	3.958IleLys: 3.958 ± 0.05	7.214IleLeu: 7.214 ± 0.065	0.875IleMet: 0.875 ± 0.021	3.805IleAsn: 3.805 ± 0.05	3.717IlePro: 3.717 ± 0.046	3.001IleGln: 3.001 ± 0.043	3.257IleArg: 3.257 ± 0.042	5.419IleSer: 5.419 ± 0.055	3.939IleThr: 3.939 ± 0.046	4.322IleVal: 4.322 ± 0.047	0.919IleTrp: 0.919 ± 0.022	2.225IleTyr: 2.225 ± 0.038	0.001IleXaa: 0.001 ± 0.001
Lys	4.183LysAla: 4.183 ± 0.057	0.43LysCys: 0.43 ± 0.016	2.718LysAsp: 2.718 ± 0.042	3.677LysGlu: 3.677 ± 0.046	2.313LysPhe: 2.313 ± 0.031	3.048LysGly: 3.048 ± 0.041	1.008LysHis: 1.008 ± 0.024	4.803LysIle: 4.803 ± 0.062	3.413LysLys: 3.413 ± 0.054	6.591LysLeu: 6.591 ± 0.071	1.043LysMet: 1.043 ± 0.021	3.116LysAsn: 3.116 ± 0.045	2.712LysPro: 2.712 ± 0.04	3.269LysGln: 3.269 ± 0.051	2.614LysArg: 2.614 ± 0.035	3.931LysSer: 3.931 ± 0.047	3.441LysThr: 3.441 ± 0.047	3.769LysVal: 3.769 ± 0.044	0.647LysTrp: 0.647 ± 0.018	1.959LysTyr: 1.959 ± 0.04	0.002LysXaa: 0.002 ± 0.001
Leu	8.243LeuAla: 8.243 ± 0.078	0.958LeuCys: 0.958 ± 0.022	5.275LeuAsp: 5.275 ± 0.052	7.682LeuGlu: 7.682 ± 0.076	3.956LeuPhe: 3.956 ± 0.051	7.283LeuGly: 7.283 ± 0.074	1.796LeuHis: 1.796 ± 0.032	7.013LeuIle: 7.013 ± 0.07	6.329LeuLys: 6.329 ± 0.068	10.661LeuLeu: 10.661 ± 0.093	1.943LeuMet: 1.943 ± 0.033	5.082LeuAsn: 5.082 ± 0.053	5.157LeuPro: 5.157 ± 0.061	5.634LeuGln: 5.634 ± 0.057	5.401LeuArg: 5.401 ± 0.058	7.699LeuSer: 7.699 ± 0.08	5.934LeuThr: 5.934 ± 0.066	6.615LeuVal: 6.615 ± 0.056	1.39LeuTrp: 1.39 ± 0.031	2.68LeuTyr: 2.68 ± 0.04	0.004LeuXaa: 0.004 ± 0.002
Met	1.293MetAla: 1.293 ± 0.027	0.12MetCys: 0.12 ± 0.008	0.712MetAsp: 0.712 ± 0.022	1.009MetGlu: 1.009 ± 0.021	0.593MetPhe: 0.593 ± 0.017	1.293MetGly: 1.293 ± 0.026	0.321MetHis: 0.321 ± 0.013	1.016MetIle: 1.016 ± 0.023	1.03MetLys: 1.03 ± 0.023	1.846MetLeu: 1.846 ± 0.029	0.399MetMet: 0.399 ± 0.015	0.884MetAsn: 0.884 ± 0.02	0.707MetPro: 0.707 ± 0.017	0.79MetGln: 0.79 ± 0.018	0.931MetArg: 0.931 ± 0.02	1.278MetSer: 1.278 ± 0.026	1.177MetThr: 1.177 ± 0.022	1.188MetVal: 1.188 ± 0.027	0.153MetTrp: 0.153 ± 0.008	0.337MetTyr: 0.337 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.027AsnAla: 3.027 ± 0.043	0.53AsnCys: 0.53 ± 0.016	2.005AsnAsp: 2.005 ± 0.038	2.145AsnGlu: 2.145 ± 0.036	2.388AsnPhe: 2.388 ± 0.036	2.982AsnGly: 2.982 ± 0.055	0.918AsnHis: 0.918 ± 0.022	3.858AsnIle: 3.858 ± 0.054	2.664AsnLys: 2.664 ± 0.043	6.456AsnLeu: 6.456 ± 0.106	0.704AsnMet: 0.704 ± 0.018	2.953AsnAsn: 2.953 ± 0.052	3.04AsnPro: 3.04 ± 0.044	2.874AsnGln: 2.874 ± 0.041	2.531AsnArg: 2.531 ± 0.038	4.144AsnSer: 4.144 ± 0.056	2.752AsnThr: 2.752 ± 0.039	2.354AsnVal: 2.354 ± 0.036	0.886AsnTrp: 0.886 ± 0.021	1.818AsnTyr: 1.818 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	2.473ProAla: 2.473 ± 0.038	0.333ProCys: 0.333 ± 0.014	2.646ProAsp: 2.646 ± 0.046	3.746ProGlu: 3.746 ± 0.046	1.71ProPhe: 1.71 ± 0.03	2.765ProGly: 2.765 ± 0.034	0.826ProHis: 0.826 ± 0.02	3.001ProIle: 3.001 ± 0.041	2.547ProLys: 2.547 ± 0.036	4.187ProLeu: 4.187 ± 0.048	0.647ProMet: 0.647 ± 0.018	2.284ProAsn: 2.284 ± 0.033	1.864ProPro: 1.864 ± 0.037	2.435ProGln: 2.435 ± 0.034	1.665ProArg: 1.665 ± 0.032	2.806ProSer: 2.806 ± 0.04	2.415ProThr: 2.415 ± 0.038	3.004ProVal: 3.004 ± 0.046	0.526ProTrp: 0.526 ± 0.016	1.218ProTyr: 1.218 ± 0.024	0.002ProXaa: 0.002 ± 0.001
Gln	3.92GlnAla: 3.92 ± 0.051	0.306GlnCys: 0.306 ± 0.012	2.137GlnAsp: 2.137 ± 0.035	3.631GlnGlu: 3.631 ± 0.046	1.589GlnPhe: 1.589 ± 0.025	2.979GlnGly: 2.979 ± 0.043	0.758GlnHis: 0.758 ± 0.022	3.72GlnIle: 3.72 ± 0.04	3.327GlnLys: 3.327 ± 0.047	5.307GlnLeu: 5.307 ± 0.068	0.895GlnMet: 0.895 ± 0.02	2.504GlnAsn: 2.504 ± 0.038	2.043GlnPro: 2.043 ± 0.034	3.13GlnGln: 3.13 ± 0.055	2.543GlnArg: 2.543 ± 0.038	2.905GlnSer: 2.905 ± 0.047	2.807GlnThr: 2.807 ± 0.037	3.434GlnVal: 3.434 ± 0.042	0.585GlnTrp: 0.585 ± 0.017	1.17GlnTyr: 1.17 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	2.948ArgAla: 2.948 ± 0.043	0.491ArgCys: 0.491 ± 0.016	2.547ArgAsp: 2.547 ± 0.035	3.307ArgGlu: 3.307 ± 0.042	2.089ArgPhe: 2.089 ± 0.032	2.938ArgGly: 2.938 ± 0.043	0.884ArgHis: 0.884 ± 0.023	3.47ArgIle: 3.47 ± 0.041	2.918ArgLys: 2.918 ± 0.044	5.423ArgLeu: 5.423 ± 0.058	0.936ArgMet: 0.936 ± 0.02	2.474ArgAsn: 2.474 ± 0.036	1.798ArgPro: 1.798 ± 0.033	2.764ArgGln: 2.764 ± 0.041	2.958ArgArg: 2.958 ± 0.047	3.234ArgSer: 3.234 ± 0.038	2.371ArgThr: 2.371 ± 0.037	3.231ArgVal: 3.231 ± 0.039	0.71ArgTrp: 0.71 ± 0.022	1.816ArgTyr: 1.816 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	4.337SerAla: 4.337 ± 0.051	0.66SerCys: 0.66 ± 0.019	3.385SerAsp: 3.385 ± 0.042	4.413SerGlu: 4.413 ± 0.059	2.962SerPhe: 2.962 ± 0.048	4.929SerGly: 4.929 ± 0.065	1.284SerHis: 1.284 ± 0.025	4.487SerIle: 4.487 ± 0.05	3.716SerLys: 3.716 ± 0.049	7.606SerLeu: 7.606 ± 0.071	1.176SerMet: 1.176 ± 0.024	3.493SerAsn: 3.493 ± 0.05	3.355SerPro: 3.355 ± 0.053	3.922SerGln: 3.922 ± 0.05	3.221SerArg: 3.221 ± 0.043	5.416SerSer: 5.416 ± 0.057	3.644SerThr: 3.644 ± 0.046	4.079SerVal: 4.079 ± 0.054	0.927SerTrp: 0.927 ± 0.023	2.018SerTyr: 2.018 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	4.13ThrAla: 4.13 ± 0.055	0.47ThrCys: 0.47 ± 0.016	2.738ThrAsp: 2.738 ± 0.042	3.403ThrGlu: 3.403 ± 0.042	2.141ThrPhe: 2.141 ± 0.032	4.055ThrGly: 4.055 ± 0.052	0.916ThrHis: 0.916 ± 0.019	3.907ThrIle: 3.907 ± 0.056	2.8ThrLys: 2.8 ± 0.034	5.81ThrLeu: 5.81 ± 0.059	0.729ThrMet: 0.729 ± 0.02	2.666ThrAsn: 2.666 ± 0.046	2.999ThrPro: 2.999 ± 0.039	2.795ThrGln: 2.795 ± 0.052	2.23ThrArg: 2.23 ± 0.036	3.755ThrSer: 3.755 ± 0.049	3.227ThrThr: 3.227 ± 0.053	3.705ThrVal: 3.705 ± 0.052	0.665ThrTrp: 0.665 ± 0.02	1.577ThrTyr: 1.577 ± 0.029	0.001ThrXaa: 0.001 ± 0.001
Val	4.982ValAla: 4.982 ± 0.054	0.65ValCys: 0.65 ± 0.021	3.357ValAsp: 3.357 ± 0.042	4.502ValGlu: 4.502 ± 0.047	2.573ValPhe: 2.573 ± 0.04	4.318ValGly: 4.318 ± 0.053	0.937ValHis: 0.937 ± 0.023	4.38ValIle: 4.38 ± 0.055	3.755ValLys: 3.755 ± 0.051	6.208ValLeu: 6.208 ± 0.057	1.231ValMet: 1.231 ± 0.025	3.24ValAsn: 3.24 ± 0.043	2.476ValPro: 2.476 ± 0.039	2.374ValGln: 2.374 ± 0.037	3.21ValArg: 3.21 ± 0.049	4.298ValSer: 4.298 ± 0.047	3.683ValThr: 3.683 ± 0.049	4.409ValVal: 4.409 ± 0.057	0.81ValTrp: 0.81 ± 0.02	1.768ValTyr: 1.768 ± 0.031	0.002ValXaa: 0.002 ± 0.001
Trp	0.716TrpAla: 0.716 ± 0.02	0.156TrpCys: 0.156 ± 0.009	0.734TrpAsp: 0.734 ± 0.024	1.101TrpGlu: 1.101 ± 0.024	0.619TrpPhe: 0.619 ± 0.017	0.953TrpGly: 0.953 ± 0.022	0.295TrpHis: 0.295 ± 0.014	0.823TrpIle: 0.823 ± 0.02	0.766TrpLys: 0.766 ± 0.019	1.75TrpLeu: 1.75 ± 0.029	0.294TrpMet: 0.294 ± 0.012	0.717TrpAsn: 0.717 ± 0.023	0.15TrpPro: 0.15 ± 0.009	0.836TrpGln: 0.836 ± 0.023	0.833TrpArg: 0.833 ± 0.02	0.851TrpSer: 0.851 ± 0.022	0.532TrpThr: 0.532 ± 0.016	0.908TrpVal: 0.908 ± 0.026	0.248TrpTrp: 0.248 ± 0.012	0.447TrpTyr: 0.447 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.924TyrAla: 1.924 ± 0.033	0.385TyrCys: 0.385 ± 0.015	1.471TyrAsp: 1.471 ± 0.034	1.791TyrGlu: 1.791 ± 0.034	1.426TyrPhe: 1.426 ± 0.033	1.952TyrGly: 1.952 ± 0.032	0.669TyrHis: 0.669 ± 0.02	1.887TyrIle: 1.887 ± 0.036	1.576TyrLys: 1.576 ± 0.033	3.619TyrLeu: 3.619 ± 0.043	0.433TyrMet: 0.433 ± 0.014	1.359TyrAsn: 1.359 ± 0.029	1.592TyrPro: 1.592 ± 0.026	2.016TyrGln: 2.016 ± 0.033	1.904TyrArg: 1.904 ± 0.03	2.133TyrSer: 2.133 ± 0.031	1.479TyrThr: 1.479 ± 0.03	1.58TyrVal: 1.58 ± 0.028	0.534TyrTrp: 0.534 ± 0.016	1.069TyrTyr: 1.069 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.002	0.0XaaHis: 0.0 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.004XaaLeu: 0.004 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 6353 proteins (2258355 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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