Bovine papular stomatitis virus
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6TV73|Q6TV73_9POXV Uncharacterized protein OS=Bovine papular stomatitis virus OX=129727 GN=BVTX09c15_115 PE=4 SV=1
MM1 pKa = 7.17 IHH3 pKa = 6.54 FLLRR7 pKa = 11.84 HH8 pKa = 5.51 SLFLYY13 pKa = 9.06 WFIEE17 pKa = 3.54 PRR19 pKa = 11.84 YY20 pKa = 8.76 PHH22 pKa = 6.9 TNTLGKK28 pKa = 9.65 MSEE31 pKa = 4.34 TAPSSSTTDD40 pKa = 3.02 STSAGTSDD48 pKa = 4.73 ASTSTNDD55 pKa = 3.02 STAMSSAPTTDD66 pKa = 3.35 ASSTSTTSNTSSATTSSSSDD86 pKa = 3.48 TEE88 pKa = 4.45 SSSTTANNADD98 pKa = 3.85 SGSATTTTTTTEE110 pKa = 4.09 STPTASTGSEE120 pKa = 4.12 STSSATQPTTTDD132 pKa = 3.62 PTPDD136 pKa = 4.16 PAPVPATAPTASTGSEE152 pKa = 4.12 STSSATQPTTTVPTTTDD169 pKa = 3.62 PTPDD173 pKa = 4.2 PAPAPAPAPAPAPAPMPNPVTTTTTTVSTTATNGTNRR210 pKa = 11.84 CEE212 pKa = 4.26 CVCTCTTTMTGGNQYY227 pKa = 10.27 PPEE230 pKa = 5.36 NIDD233 pKa = 3.9 LGNSASTPSPPDD245 pKa = 3.23 VEE247 pKa = 4.46 EE248 pKa = 4.7 PPAEE252 pKa = 4.04 EE253 pKa = 4.41 DD254 pKa = 3.68 EE255 pKa = 4.44 EE256 pKa = 4.97 VII258 pKa = 5.15
Molecular weight: 25.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.465
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.503
Grimsley 3.376
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.516
EMBOSS 3.579
Sillero 3.783
Patrickios 0.604
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|Q6TVC0|Q6TVC0_9POXV Primase D5 OS=Bovine papular stomatitis virus OX=129727 GN=BVTX09c1_068 PE=3 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 SYY4 pKa = 11.29 TSLLSKK10 pKa = 10.13 LVKK13 pKa = 10.32 ANRR16 pKa = 11.84 RR17 pKa = 11.84 LGSTRR22 pKa = 11.84 VFRR25 pKa = 11.84 DD26 pKa = 3.33 PLQHH30 pKa = 6.51 ISATAFVYY38 pKa = 10.37 RR39 pKa = 11.84 RR40 pKa = 11.84 IDD42 pKa = 3.14 RR43 pKa = 11.84 YY44 pKa = 10.64 RR45 pKa = 11.84 RR46 pKa = 11.84 ISICAVLTTTDD57 pKa = 3.8 GLVVACRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 LSFLSTEE73 pKa = 4.01 LAEE76 pKa = 4.29 TRR78 pKa = 11.84 SAARR82 pKa = 11.84 RR83 pKa = 11.84 VMLAARR89 pKa = 11.84 HH90 pKa = 5.84 ADD92 pKa = 3.11 ALSRR96 pKa = 11.84 IGASRR101 pKa = 11.84 SRR103 pKa = 11.84 DD104 pKa = 2.99 DD105 pKa = 3.89 VMFPGGAPLPGEE117 pKa = 4.23 TFVDD121 pKa = 4.21 CVTRR125 pKa = 11.84 EE126 pKa = 4.05 VEE128 pKa = 4.03 EE129 pKa = 4.02 EE130 pKa = 3.66 TGIRR134 pKa = 11.84 RR135 pKa = 11.84 DD136 pKa = 3.26 QMRR139 pKa = 11.84 IDD141 pKa = 3.26 EE142 pKa = 4.54 RR143 pKa = 11.84 MFVHH147 pKa = 7.21 AFIDD151 pKa = 3.84 DD152 pKa = 4.24 LVTGRR157 pKa = 11.84 DD158 pKa = 3.15 FDD160 pKa = 5.78 AIIFTGDD167 pKa = 3.3 LRR169 pKa = 11.84 LSSAEE174 pKa = 3.91 VEE176 pKa = 4.3 RR177 pKa = 11.84 QFVPNGEE184 pKa = 4.37 VKK186 pKa = 10.64 GLLFLRR192 pKa = 11.84 PTEE195 pKa = 4.21 DD196 pKa = 2.74 AGGVMARR203 pKa = 11.84 LAAFARR209 pKa = 11.84 AAARR213 pKa = 11.84 MRR215 pKa = 11.84 CWGSAATRR223 pKa = 3.58
Molecular weight: 24.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.297
IPC_protein 10.452
Toseland 10.438
ProMoST 10.716
Dawson 10.555
Bjellqvist 10.409
Wikipedia 10.862
Rodwell 10.438
Grimsley 10.628
Solomon 10.774
Lehninger 10.73
Nozaki 10.496
DTASelect 10.379
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.131
IPC_peptide 10.774
IPC2_peptide 9.838
IPC2.peptide.svr19 8.838
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
130
0
130
42358
53
1289
325.8
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.736 ± 0.316
2.373 ± 0.125
6.233 ± 0.117
5.69 ± 0.123
4.445 ± 0.157
5.352 ± 0.147
2.297 ± 0.103
4.174 ± 0.168
3.631 ± 0.165
9.542 ± 0.202
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.883 ± 0.095
3.485 ± 0.14
4.776 ± 0.152
2.122 ± 0.095
8.006 ± 0.198
7.042 ± 0.208
5.645 ± 0.163
8.86 ± 0.171
0.67 ± 0.059
3.038 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here