Microbotryum intermedium

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Microbotryales; Microbotryaceae; Microbotryum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8013 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238F381|A0A238F381_9BASI BQ2448_5118 protein OS=Microbotryum intermedium OX=269621 GN=BQ2448_5118 PE=4 SV=1
MM1 pKa = 7.65ACNTVNMFASACSSPTVVTACIGPYY26 pKa = 8.91TVSADD31 pKa = 3.48KK32 pKa = 10.71KK33 pKa = 9.45SCVLGTAWSIYY44 pKa = 9.74LLSSIPDD51 pKa = 3.38TPYY54 pKa = 10.31QSNIRR59 pKa = 11.84SPGACGNRR67 pKa = 11.84AVAKK71 pKa = 8.78GAQVAVFDD79 pKa = 4.54SGSLTCYY86 pKa = 10.75ASTANNSVSTMANLEE101 pKa = 3.95WDD103 pKa = 4.03LDD105 pKa = 3.96STTIVYY111 pKa = 7.55GTCKK115 pKa = 10.12KK116 pKa = 10.9NNVLNPAARR125 pKa = 11.84GRR127 pKa = 11.84CHH129 pKa = 7.13DD130 pKa = 4.41VILTTSACNLVADD143 pKa = 4.94KK144 pKa = 9.53STCSTLFAPCEE155 pKa = 3.92GDD157 pKa = 3.39QFRR160 pKa = 11.84NYY162 pKa = 10.95NGICQDD168 pKa = 3.4CDD170 pKa = 3.24EE171 pKa = 5.29VYY173 pKa = 10.92DD174 pKa = 4.48GALTCDD180 pKa = 3.4IFNGATSCIAGDD192 pKa = 3.71YY193 pKa = 7.52LTNGTCSSCSDD204 pKa = 4.33FDD206 pKa = 4.57PNAANCSADD215 pKa = 3.46GTVDD219 pKa = 3.11SCNDD223 pKa = 2.79GWVLSGNTCIADD235 pKa = 3.7TSSVPQIGLDD245 pKa = 4.0TAWSYY250 pKa = 11.92YY251 pKa = 9.11PDD253 pKa = 3.54SSISNLAALAVFNTNSTDD271 pKa = 3.43QYY273 pKa = 11.24DD274 pKa = 4.19CAEE277 pKa = 4.21SVYY280 pKa = 11.16DD281 pKa = 4.14SNFTMAVFGPTSQKK295 pKa = 10.71CYY297 pKa = 9.71ATNATNTLTNSIIDD311 pKa = 3.88LDD313 pKa = 4.03GASVFVIEE321 pKa = 4.68VCAANQAQVPYY332 pKa = 10.22NQGNTCYY339 pKa = 10.66DD340 pKa = 2.94ITLDD344 pKa = 4.81SEE346 pKa = 4.69SCQVNGGSCNN356 pKa = 3.45

Molecular weight: 37.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238F5F9|A0A238F5F9_9BASI BQ2448_5837 protein OS=Microbotryum intermedium OX=269621 GN=BQ2448_5837 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84SRR4 pKa = 11.84NPSSIRR10 pKa = 11.84LDD12 pKa = 4.18LIFGLYY18 pKa = 10.11LDD20 pKa = 4.54IVAGLSDD27 pKa = 3.47VRR29 pKa = 11.84IRR31 pKa = 11.84LLAANQPHH39 pKa = 7.61PFTLRR44 pKa = 11.84KK45 pKa = 9.42PPPNQHH51 pKa = 5.45RR52 pKa = 11.84RR53 pKa = 11.84PWIWIWWAYY62 pKa = 9.23FCHH65 pKa = 6.84PPPKK69 pKa = 9.07WYY71 pKa = 9.65HH72 pKa = 5.91AANHH76 pKa = 5.83VLFFPAGHH84 pKa = 7.27GIQLSVNPTLFRR96 pKa = 11.84GPDD99 pKa = 3.8GEE101 pKa = 4.81VMTPTSFIDD110 pKa = 3.48ASKK113 pKa = 10.32RR114 pKa = 11.84RR115 pKa = 11.84HH116 pKa = 5.8AFVFPPIIPEE126 pKa = 4.0GHH128 pKa = 5.45QKK130 pKa = 9.92IFFLTEE136 pKa = 3.75QRR138 pKa = 11.84WKK140 pKa = 10.58SWWKK144 pKa = 9.48ALRR147 pKa = 11.84KK148 pKa = 8.97IRR150 pKa = 11.84RR151 pKa = 11.84VHH153 pKa = 7.13SDD155 pKa = 3.32AEE157 pKa = 4.4DD158 pKa = 3.65TAHH161 pKa = 7.14LLSLGSLHH169 pKa = 7.08PGVQLSSATHH179 pKa = 5.28SQLNNRR185 pKa = 11.84SASYY189 pKa = 10.56VMCLSEE195 pKa = 4.87APEE198 pKa = 4.37SLPHH202 pKa = 6.36LAVGCTFARR211 pKa = 11.84RR212 pKa = 11.84LWAALSPSPHH222 pKa = 7.21PIFADD227 pKa = 4.87FVCPLVSRR235 pKa = 11.84SEE237 pKa = 3.86RR238 pKa = 11.84RR239 pKa = 11.84IVEE242 pKa = 3.64LRR244 pKa = 11.84ILFFHH249 pKa = 6.86SIWKK253 pKa = 9.43LSRR256 pKa = 11.84RR257 pKa = 11.84RR258 pKa = 11.84RR259 pKa = 11.84FSSQPLEE266 pKa = 4.82PITEE270 pKa = 4.29AEE272 pKa = 4.13LEE274 pKa = 4.14EE275 pKa = 4.41LRR277 pKa = 11.84GSIQGCTGLYY287 pKa = 9.97NISKK291 pKa = 10.46GGFSGYY297 pKa = 9.91GRR299 pKa = 11.84LWLKK303 pKa = 10.73GRR305 pKa = 4.01

Molecular weight: 35.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8013

0

8013

4386583

34

5376

547.4

59.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.405 ± 0.026

1.162 ± 0.014

5.521 ± 0.019

5.811 ± 0.023

3.447 ± 0.015

6.762 ± 0.023

2.523 ± 0.012

4.275 ± 0.015

4.493 ± 0.025

9.21 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.009

3.124 ± 0.012

6.459 ± 0.032

3.757 ± 0.018

6.493 ± 0.021

9.56 ± 0.042

6.364 ± 0.019

6.136 ± 0.018

1.296 ± 0.009

2.282 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski