Amino acid dipepetide frequency for Microbotryum intermedium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.296AlaAla: 10.296 ± 0.081	1.078AlaCys: 1.078 ± 0.022	4.125AlaAsp: 4.125 ± 0.039	4.788AlaGlu: 4.788 ± 0.044	3.251AlaPhe: 3.251 ± 0.029	5.697AlaGly: 5.697 ± 0.041	2.17AlaHis: 2.17 ± 0.02	4.012AlaIle: 4.012 ± 0.028	4.143AlaLys: 4.143 ± 0.041	8.74AlaLeu: 8.74 ± 0.06	1.963AlaMet: 1.963 ± 0.022	2.944AlaAsn: 2.944 ± 0.028	6.259AlaPro: 6.259 ± 0.05	3.676AlaGln: 3.676 ± 0.035	5.991AlaArg: 5.991 ± 0.041	10.098AlaSer: 10.098 ± 0.075	6.466AlaThr: 6.466 ± 0.039	5.218AlaVal: 5.218 ± 0.041	1.113AlaTrp: 1.113 ± 0.016	2.02AlaTyr: 2.02 ± 0.023	0.0AlaXaa: 0.0 ± 0.0
Cys	0.937CysAla: 0.937 ± 0.019	0.214CysCys: 0.214 ± 0.008	0.628CysAsp: 0.628 ± 0.02	0.55CysGlu: 0.55 ± 0.013	0.503CysPhe: 0.503 ± 0.011	0.781CysGly: 0.781 ± 0.02	0.297CysHis: 0.297 ± 0.018	0.527CysIle: 0.527 ± 0.012	0.526CysLys: 0.526 ± 0.012	1.191CysLeu: 1.191 ± 0.02	0.196CysMet: 0.196 ± 0.007	0.369CysAsn: 0.369 ± 0.015	0.721CysPro: 0.721 ± 0.016	0.425CysGln: 0.425 ± 0.013	0.705CysArg: 0.705 ± 0.013	1.022CysSer: 1.022 ± 0.02	0.744CysThr: 0.744 ± 0.019	0.79CysVal: 0.79 ± 0.019	0.171CysTrp: 0.171 ± 0.006	0.327CysTyr: 0.327 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	4.975AspAla: 4.975 ± 0.042	0.554AspCys: 0.554 ± 0.021	5.13AspAsp: 5.13 ± 0.072	4.868AspGlu: 4.868 ± 0.052	1.863AspPhe: 1.863 ± 0.024	3.893AspGly: 3.893 ± 0.035	1.415AspHis: 1.415 ± 0.02	2.029AspIle: 2.029 ± 0.023	2.16AspLys: 2.16 ± 0.027	5.207AspLeu: 5.207 ± 0.036	0.897AspMet: 0.897 ± 0.012	1.304AspAsn: 1.304 ± 0.018	3.501AspPro: 3.501 ± 0.029	2.007AspGln: 2.007 ± 0.023	3.292AspArg: 3.292 ± 0.032	4.072AspSer: 4.072 ± 0.039	2.46AspThr: 2.46 ± 0.023	3.469AspVal: 3.469 ± 0.028	0.804AspTrp: 0.804 ± 0.014	1.309AspTyr: 1.309 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.517GluAla: 5.517 ± 0.042	0.578GluCys: 0.578 ± 0.012	3.937GluAsp: 3.937 ± 0.044	5.262GluGlu: 5.262 ± 0.062	1.697GluPhe: 1.697 ± 0.02	4.074GluGly: 4.074 ± 0.034	1.367GluHis: 1.367 ± 0.018	2.404GluIle: 2.404 ± 0.027	2.819GluLys: 2.819 ± 0.034	5.307GluLeu: 5.307 ± 0.045	1.408GluMet: 1.408 ± 0.019	1.58GluAsn: 1.58 ± 0.023	2.688GluPro: 2.688 ± 0.029	2.486GluGln: 2.486 ± 0.028	4.288GluArg: 4.288 ± 0.04	3.964GluSer: 3.964 ± 0.032	2.996GluThr: 2.996 ± 0.03	3.57GluVal: 3.57 ± 0.032	0.882GluTrp: 0.882 ± 0.015	1.291GluTyr: 1.291 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.217PheAla: 3.217 ± 0.029	0.497PheCys: 0.497 ± 0.011	2.304PheAsp: 2.304 ± 0.023	2.154PheGlu: 2.154 ± 0.022	1.416PhePhe: 1.416 ± 0.018	2.703PheGly: 2.703 ± 0.034	0.856PheHis: 0.856 ± 0.013	1.516PheIle: 1.516 ± 0.019	1.438PheLys: 1.438 ± 0.021	3.12PheLeu: 3.12 ± 0.035	0.583PheMet: 0.583 ± 0.01	1.132PheAsn: 1.132 ± 0.018	1.848PhePro: 1.848 ± 0.023	1.142PheGln: 1.142 ± 0.016	1.752PheArg: 1.752 ± 0.02	2.89PheSer: 2.89 ± 0.026	1.958PheThr: 1.958 ± 0.025	2.567PheVal: 2.567 ± 0.026	0.51PheTrp: 0.51 ± 0.012	0.863PheTyr: 0.863 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	6.066GlyAla: 6.066 ± 0.045	0.811GlyCys: 0.811 ± 0.016	3.402GlyAsp: 3.402 ± 0.033	3.675GlyGlu: 3.675 ± 0.033	2.517GlyPhe: 2.517 ± 0.03	6.297GlyGly: 6.297 ± 0.062	1.712GlyHis: 1.712 ± 0.021	2.791GlyIle: 2.791 ± 0.031	3.416GlyLys: 3.416 ± 0.035	5.97GlyLeu: 5.97 ± 0.043	1.461GlyMet: 1.461 ± 0.021	1.996GlyAsn: 1.996 ± 0.024	3.487GlyPro: 3.487 ± 0.032	2.447GlyGln: 2.447 ± 0.027	4.275GlyArg: 4.275 ± 0.039	6.237GlySer: 6.237 ± 0.054	4.039GlyThr: 4.039 ± 0.037	4.296GlyVal: 4.296 ± 0.036	1.05GlyTrp: 1.05 ± 0.017	1.67GlyTyr: 1.67 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	2.197HisAla: 2.197 ± 0.023	0.344HisCys: 0.344 ± 0.012	1.427HisAsp: 1.427 ± 0.026	1.258HisGlu: 1.258 ± 0.018	0.963HisPhe: 0.963 ± 0.016	1.609HisGly: 1.609 ± 0.021	1.136HisHis: 1.136 ± 0.022	0.947HisIle: 0.947 ± 0.015	0.862HisLys: 0.862 ± 0.015	2.693HisLeu: 2.693 ± 0.025	0.364HisMet: 0.364 ± 0.008	0.668HisAsn: 0.668 ± 0.013	1.96HisPro: 1.96 ± 0.023	1.072HisGln: 1.072 ± 0.016	1.742HisArg: 1.742 ± 0.021	2.204HisSer: 2.204 ± 0.028	1.244HisThr: 1.244 ± 0.016	1.564HisVal: 1.564 ± 0.017	0.303HisTrp: 0.303 ± 0.008	0.673HisTyr: 0.673 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.236IleAla: 4.236 ± 0.033	0.54IleCys: 0.54 ± 0.013	2.832IleAsp: 2.832 ± 0.024	2.83IleGlu: 2.83 ± 0.026	1.517IlePhe: 1.517 ± 0.02	2.807IleGly: 2.807 ± 0.029	0.993IleHis: 0.993 ± 0.013	1.907IleIle: 1.907 ± 0.021	2.028IleLys: 2.028 ± 0.024	3.742IleLeu: 3.742 ± 0.031	0.752IleMet: 0.752 ± 0.011	1.465IleAsn: 1.465 ± 0.019	2.721IlePro: 2.721 ± 0.023	1.484IleGln: 1.484 ± 0.02	2.545IleArg: 2.545 ± 0.028	3.308IleSer: 3.308 ± 0.026	2.339IleThr: 2.339 ± 0.025	3.092IleVal: 3.092 ± 0.028	0.599IleTrp: 0.599 ± 0.013	1.014IleTyr: 1.014 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.468LysAla: 4.468 ± 0.042	0.469LysCys: 0.469 ± 0.014	2.364LysAsp: 2.364 ± 0.028	2.991LysGlu: 2.991 ± 0.03	1.236LysPhe: 1.236 ± 0.018	3.17LysGly: 3.17 ± 0.032	1.014LysHis: 1.014 ± 0.015	2.002LysIle: 2.002 ± 0.025	3.083LysLys: 3.083 ± 0.047	3.89LysLeu: 3.89 ± 0.034	0.961LysMet: 0.961 ± 0.015	1.371LysAsn: 1.371 ± 0.017	2.352LysPro: 2.352 ± 0.026	1.588LysGln: 1.588 ± 0.021	3.447LysArg: 3.447 ± 0.037	3.315LysSer: 3.315 ± 0.03	2.697LysThr: 2.697 ± 0.024	2.95LysVal: 2.95 ± 0.033	0.587LysTrp: 0.587 ± 0.013	0.978LysTyr: 0.978 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	8.941LeuAla: 8.941 ± 0.049	1.135LeuCys: 1.135 ± 0.018	5.351LeuAsp: 5.351 ± 0.04	5.384LeuGlu: 5.384 ± 0.042	3.281LeuPhe: 3.281 ± 0.033	6.288LeuGly: 6.288 ± 0.04	2.377LeuHis: 2.377 ± 0.025	3.775LeuIle: 3.775 ± 0.035	3.953LeuLys: 3.953 ± 0.033	8.767LeuLeu: 8.767 ± 0.059	1.665LeuMet: 1.665 ± 0.022	2.952LeuAsn: 2.952 ± 0.024	5.98LeuPro: 5.98 ± 0.038	3.472LeuGln: 3.472 ± 0.031	6.082LeuArg: 6.082 ± 0.047	8.367LeuSer: 8.367 ± 0.054	5.177LeuThr: 5.177 ± 0.035	5.996LeuVal: 5.996 ± 0.046	1.072LeuTrp: 1.072 ± 0.017	2.087LeuTyr: 2.087 ± 0.022	0.0LeuXaa: 0.0 ± 0.0
Met	1.821MetAla: 1.821 ± 0.022	0.233MetCys: 0.233 ± 0.007	1.045MetAsp: 1.045 ± 0.016	0.965MetGlu: 0.965 ± 0.016	0.586MetPhe: 0.586 ± 0.012	1.342MetGly: 1.342 ± 0.021	0.444MetHis: 0.444 ± 0.011	0.845MetIle: 0.845 ± 0.013	0.78MetLys: 0.78 ± 0.015	1.737MetLeu: 1.737 ± 0.025	0.525MetMet: 0.525 ± 0.011	0.608MetAsn: 0.608 ± 0.011	1.11MetPro: 1.11 ± 0.017	0.747MetGln: 0.747 ± 0.014	1.286MetArg: 1.286 ± 0.017	1.863MetSer: 1.863 ± 0.023	1.399MetThr: 1.399 ± 0.015	1.179MetVal: 1.179 ± 0.018	0.225MetTrp: 0.225 ± 0.007	0.451MetTyr: 0.451 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.09AsnAla: 3.09 ± 0.031	0.355AsnCys: 0.355 ± 0.015	1.791AsnAsp: 1.791 ± 0.02	1.86AsnGlu: 1.86 ± 0.023	0.986AsnPhe: 0.986 ± 0.017	2.632AsnGly: 2.632 ± 0.033	0.759AsnHis: 0.759 ± 0.014	1.249AsnIle: 1.249 ± 0.018	1.418AsnLys: 1.418 ± 0.022	2.838AsnLeu: 2.838 ± 0.026	0.509AsnMet: 0.509 ± 0.011	1.168AsnAsn: 1.168 ± 0.023	2.12AsnPro: 2.12 ± 0.023	1.119AsnGln: 1.119 ± 0.019	1.848AsnArg: 1.848 ± 0.021	2.614AsnSer: 2.614 ± 0.035	1.676AsnThr: 1.676 ± 0.022	2.031AsnVal: 2.031 ± 0.023	0.418AsnTrp: 0.418 ± 0.009	0.755AsnTyr: 0.755 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.635ProAla: 5.635 ± 0.045	0.568ProCys: 0.568 ± 0.011	2.815ProAsp: 2.815 ± 0.026	3.019ProGlu: 3.019 ± 0.027	2.185ProPhe: 2.185 ± 0.024	3.508ProGly: 3.508 ± 0.035	1.562ProHis: 1.562 ± 0.021	2.786ProIle: 2.786 ± 0.028	2.606ProLys: 2.606 ± 0.035	5.638ProLeu: 5.638 ± 0.044	1.11ProMet: 1.11 ± 0.017	2.087ProAsn: 2.087 ± 0.025	6.21ProPro: 6.21 ± 0.067	2.271ProGln: 2.271 ± 0.029	3.859ProArg: 3.859 ± 0.036	8.026ProSer: 8.026 ± 0.067	5.192ProThr: 5.192 ± 0.04	3.559ProVal: 3.559 ± 0.031	0.605ProTrp: 0.605 ± 0.011	1.346ProTyr: 1.346 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.854GlnAla: 3.854 ± 0.037	0.415GlnCys: 0.415 ± 0.016	1.883GlnAsp: 1.883 ± 0.024	2.07GlnGlu: 2.07 ± 0.025	1.098GlnPhe: 1.098 ± 0.014	2.565GlnGly: 2.565 ± 0.026	1.24GlnHis: 1.24 ± 0.02	1.573GlnIle: 1.573 ± 0.019	1.403GlnLys: 1.403 ± 0.019	3.563GlnLeu: 3.563 ± 0.032	0.726GlnMet: 0.726 ± 0.015	1.066GlnAsn: 1.066 ± 0.017	2.243GlnPro: 2.243 ± 0.032	2.579GlnGln: 2.579 ± 0.078	2.705GlnArg: 2.705 ± 0.028	2.844GlnSer: 2.844 ± 0.03	2.043GlnThr: 2.043 ± 0.023	2.397GlnVal: 2.397 ± 0.022	0.477GlnTrp: 0.477 ± 0.011	0.826GlnTyr: 0.826 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	5.719ArgAla: 5.719 ± 0.049	0.729ArgCys: 0.729 ± 0.014	3.149ArgAsp: 3.149 ± 0.035	3.618ArgGlu: 3.618 ± 0.036	2.261ArgPhe: 2.261 ± 0.021	4.025ArgGly: 4.025 ± 0.041	1.697ArgHis: 1.697 ± 0.021	2.965ArgIle: 2.965 ± 0.03	3.279ArgLys: 3.279 ± 0.028	6.135ArgLeu: 6.135 ± 0.05	1.343ArgMet: 1.343 ± 0.019	1.926ArgAsn: 1.926 ± 0.023	4.007ArgPro: 4.007 ± 0.036	2.448ArgGln: 2.448 ± 0.023	5.683ArgArg: 5.683 ± 0.055	6.075ArgSer: 6.075 ± 0.051	3.753ArgThr: 3.753 ± 0.03	3.662ArgVal: 3.662 ± 0.027	0.969ArgTrp: 0.969 ± 0.016	1.487ArgTyr: 1.487 ± 0.017	0.0ArgXaa: 0.0 ± 0.0
Ser	8.467SerAla: 8.467 ± 0.057	0.954SerCys: 0.954 ± 0.021	4.45SerAsp: 4.45 ± 0.034	3.936SerGlu: 3.936 ± 0.036	3.259SerPhe: 3.259 ± 0.032	5.774SerGly: 5.774 ± 0.046	2.347SerHis: 2.347 ± 0.03	4.213SerIle: 4.213 ± 0.035	3.879SerLys: 3.879 ± 0.033	8.398SerLeu: 8.398 ± 0.054	1.703SerMet: 1.703 ± 0.021	3.317SerAsn: 3.317 ± 0.036	6.784SerPro: 6.784 ± 0.076	3.026SerGln: 3.026 ± 0.031	5.611SerArg: 5.611 ± 0.047	13.614SerSer: 13.614 ± 0.13	8.207SerThr: 8.207 ± 0.071	4.776SerVal: 4.776 ± 0.037	1.001SerTrp: 1.001 ± 0.017	1.886SerTyr: 1.886 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	5.625ThrAla: 5.625 ± 0.036	0.834ThrCys: 0.834 ± 0.023	2.592ThrAsp: 2.592 ± 0.028	2.726ThrGlu: 2.726 ± 0.025	2.242ThrPhe: 2.242 ± 0.025	3.867ThrGly: 3.867 ± 0.032	1.433ThrHis: 1.433 ± 0.016	2.958ThrIle: 2.958 ± 0.028	2.709ThrLys: 2.709 ± 0.028	5.753ThrLeu: 5.753 ± 0.037	1.158ThrMet: 1.158 ± 0.017	2.102ThrAsn: 2.102 ± 0.021	5.051ThrPro: 5.051 ± 0.043	1.971ThrGln: 1.971 ± 0.024	3.78ThrArg: 3.78 ± 0.034	7.552ThrSer: 7.552 ± 0.067	5.721ThrThr: 5.721 ± 0.058	3.407ThrVal: 3.407 ± 0.029	0.761ThrTrp: 0.761 ± 0.013	1.402ThrTyr: 1.402 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	5.855ValAla: 5.855 ± 0.044	0.789ValCys: 0.789 ± 0.019	3.742ValAsp: 3.742 ± 0.031	4.215ValGlu: 4.215 ± 0.038	2.214ValPhe: 2.214 ± 0.025	4.175ValGly: 4.175 ± 0.033	1.463ValHis: 1.463 ± 0.018	2.539ValIle: 2.539 ± 0.029	2.835ValLys: 2.835 ± 0.028	5.882ValLeu: 5.882 ± 0.044	1.229ValMet: 1.229 ± 0.016	1.899ValAsn: 1.899 ± 0.023	3.779ValPro: 3.779 ± 0.032	2.287ValGln: 2.287 ± 0.023	3.695ValArg: 3.695 ± 0.029	4.605ValSer: 4.605 ± 0.038	3.292ValThr: 3.292 ± 0.03	4.605ValVal: 4.605 ± 0.039	0.846ValTrp: 0.846 ± 0.017	1.417ValTyr: 1.417 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.101TrpAla: 1.101 ± 0.019	0.179TrpCys: 0.179 ± 0.007	0.753TrpAsp: 0.753 ± 0.016	0.71TrpGlu: 0.71 ± 0.014	0.43TrpPhe: 0.43 ± 0.012	0.791TrpGly: 0.791 ± 0.012	0.308TrpHis: 0.308 ± 0.009	0.7TrpIle: 0.7 ± 0.014	0.702TrpLys: 0.702 ± 0.014	1.128TrpLeu: 1.128 ± 0.017	0.27TrpMet: 0.27 ± 0.01	0.503TrpAsn: 0.503 ± 0.012	0.524TrpPro: 0.524 ± 0.012	0.484TrpGln: 0.484 ± 0.011	0.891TrpArg: 0.891 ± 0.014	1.14TrpSer: 1.14 ± 0.019	0.915TrpThr: 0.915 ± 0.017	0.833TrpVal: 0.833 ± 0.014	0.265TrpTrp: 0.265 ± 0.008	0.337TrpTyr: 0.337 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.031TyrAla: 2.031 ± 0.025	0.349TyrCys: 0.349 ± 0.009	1.492TyrAsp: 1.492 ± 0.022	1.231TyrGlu: 1.231 ± 0.017	0.963TyrPhe: 0.963 ± 0.016	1.617TyrGly: 1.617 ± 0.025	0.642TyrHis: 0.642 ± 0.01	1.012TyrIle: 1.012 ± 0.016	0.891TyrLys: 0.891 ± 0.015	2.4TyrLeu: 2.4 ± 0.028	0.368TyrMet: 0.368 ± 0.01	0.779TyrAsn: 0.779 ± 0.015	1.244TyrPro: 1.244 ± 0.018	0.84TyrGln: 0.84 ± 0.013	1.457TyrArg: 1.457 ± 0.02	1.792TyrSer: 1.792 ± 0.021	1.328TyrThr: 1.328 ± 0.019	1.401TyrVal: 1.401 ± 0.019	0.304TyrTrp: 0.304 ± 0.008	0.678TyrTyr: 0.678 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8013 proteins (4386583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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