Mycobacterium phage Phayonce

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; Phayoncevirus; Mycobacterium virus Phayonce

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6WDW8|A0A0F6WDW8_9CAUD Uncharacterized protein OS=Mycobacterium phage Phayonce OX=1647302 GN=44 PE=4 SV=1
MM1 pKa = 7.64SSDD4 pKa = 3.68YY5 pKa = 11.28LDD7 pKa = 4.4ASGLTDD13 pKa = 4.59AGLDD17 pKa = 4.76DD18 pKa = 4.97EE19 pKa = 6.05FLDD22 pKa = 5.48LDD24 pKa = 4.15EE25 pKa = 6.56LDD27 pKa = 4.45LPRR30 pKa = 11.84SAEE33 pKa = 4.14PQCDD37 pKa = 4.19HH38 pKa = 7.25PPVACDD44 pKa = 3.17ATCRR48 pKa = 11.84RR49 pKa = 11.84MHH51 pKa = 6.18TAGEE55 pKa = 4.26VSTGIPRR62 pKa = 11.84TVPSPAGSTSPVPSIYY78 pKa = 10.63AAVSTVLLAVALAALVLAVFAA99 pKa = 5.37

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6SJK9|A0A0F6SJK9_9CAUD Uncharacterized protein OS=Mycobacterium phage Phayonce OX=1647302 GN=37 PE=4 SV=1
MM1 pKa = 7.42IEE3 pKa = 4.56RR4 pKa = 11.84WRR6 pKa = 11.84TWQFAQSLSRR16 pKa = 11.84RR17 pKa = 11.84TVDD20 pKa = 2.99EE21 pKa = 4.2RR22 pKa = 11.84SATVRR27 pKa = 11.84RR28 pKa = 11.84MATWCRR34 pKa = 11.84VAPEE38 pKa = 4.13LADD41 pKa = 3.52VEE43 pKa = 5.85HH44 pKa = 6.72IVTWLAEE51 pKa = 4.41GGDD54 pKa = 4.51GNWSARR60 pKa = 11.84TRR62 pKa = 11.84WTYY65 pKa = 11.34YY66 pKa = 10.31GALSAWFLWLQQQGYY81 pKa = 8.47RR82 pKa = 11.84QDD84 pKa = 3.5NPMVMIGRR92 pKa = 11.84PKK94 pKa = 10.09RR95 pKa = 11.84PKK97 pKa = 9.75SVPRR101 pKa = 11.84PVSNHH106 pKa = 5.44DD107 pKa = 3.48VQRR110 pKa = 11.84LLAVRR115 pKa = 11.84AHH117 pKa = 6.72RR118 pKa = 11.84RR119 pKa = 11.84TKK121 pKa = 11.44AMILLAAFQGLRR133 pKa = 11.84VHH135 pKa = 7.38EE136 pKa = 4.11IAQIKK141 pKa = 10.05GEE143 pKa = 4.07HH144 pKa = 6.8LDD146 pKa = 4.12LIEE149 pKa = 4.13RR150 pKa = 11.84TMTVTGKK157 pKa = 11.18GNITATLPVHH167 pKa = 5.97HH168 pKa = 6.77RR169 pKa = 11.84VVEE172 pKa = 3.95IAYY175 pKa = 8.39QMPRR179 pKa = 11.84KK180 pKa = 9.34GHH182 pKa = 6.4WFPGPDD188 pKa = 2.81RR189 pKa = 11.84GHH191 pKa = 5.67QRR193 pKa = 11.84RR194 pKa = 11.84EE195 pKa = 4.37SISGTIKK202 pKa = 10.18EE203 pKa = 4.1AMIRR207 pKa = 11.84AGVLGSAHH215 pKa = 7.05CLRR218 pKa = 11.84HH219 pKa = 4.94WFGTALLEE227 pKa = 4.69AGVDD231 pKa = 3.56LRR233 pKa = 11.84TVQEE237 pKa = 4.11LMRR240 pKa = 11.84HH241 pKa = 4.7QSLTSTEE248 pKa = 3.62IYY250 pKa = 10.3TRR252 pKa = 11.84VTDD255 pKa = 3.31QRR257 pKa = 11.84RR258 pKa = 11.84AEE260 pKa = 4.59GIEE263 pKa = 3.83RR264 pKa = 11.84LDD266 pKa = 3.98PFRR269 pKa = 11.84MSPIARR275 pKa = 11.84VHH277 pKa = 5.71EE278 pKa = 4.24RR279 pKa = 11.84LLNEE283 pKa = 5.28LLDD286 pKa = 5.03DD287 pKa = 5.61DD288 pKa = 5.13DD289 pKa = 5.06AAAASAAA296 pKa = 3.74

Molecular weight: 33.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

15953

38

1268

207.2

22.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.054 ± 0.504

0.959 ± 0.142

6.839 ± 0.175

6.011 ± 0.296

2.89 ± 0.181

9.127 ± 0.579

2.169 ± 0.175

4.413 ± 0.169

3.053 ± 0.191

7.861 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.975 ± 0.144

2.971 ± 0.16

6.243 ± 0.208

3.792 ± 0.224

7.115 ± 0.387

4.777 ± 0.228

6.381 ± 0.232

7.039 ± 0.267

1.943 ± 0.143

2.388 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski