Amino acid dipepetide frequency for Mycobacterium phage Phayonce

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.362AlaAla: 16.362 ± 1.427
1.003AlaCys: 1.003 ± 0.254
8.902AlaAsp: 8.902 ± 0.784
9.779AlaGlu: 9.779 ± 1.009
3.385AlaPhe: 3.385 ± 0.491
9.529AlaGly: 9.529 ± 1.32
2.758AlaHis: 2.758 ± 0.467
5.454AlaIle: 5.454 ± 0.501
4.263AlaLys: 4.263 ± 0.571
9.466AlaLeu: 9.466 ± 0.755
2.508AlaMet: 2.508 ± 0.294
3.134AlaAsn: 3.134 ± 0.454
6.77AlaPro: 6.77 ± 0.908
4.827AlaGln: 4.827 ± 0.662
8.087AlaArg: 8.087 ± 0.708
6.018AlaSer: 6.018 ± 0.639
6.52AlaThr: 6.52 ± 0.8
7.335AlaVal: 7.335 ± 0.602
1.881AlaTrp: 1.881 ± 0.408
2.508AlaTyr: 2.508 ± 0.342
0.0AlaXaa: 0.0 ± 0.0
Cys
0.94CysAla: 0.94 ± 0.267
0.063CysCys: 0.063 ± 0.064
0.627CysAsp: 0.627 ± 0.193
0.564CysGlu: 0.564 ± 0.184
0.063CysPhe: 0.063 ± 0.057
1.505CysGly: 1.505 ± 0.352
0.439CysHis: 0.439 ± 0.149
0.188CysIle: 0.188 ± 0.1
0.125CysLys: 0.125 ± 0.076
0.502CysLeu: 0.502 ± 0.19
0.0CysMet: 0.0 ± 0.0
0.188CysAsn: 0.188 ± 0.107
0.815CysPro: 0.815 ± 0.263
0.376CysGln: 0.376 ± 0.161
1.254CysArg: 1.254 ± 0.275
0.439CysSer: 0.439 ± 0.21
0.752CysThr: 0.752 ± 0.208
0.313CysVal: 0.313 ± 0.157
0.188CysTrp: 0.188 ± 0.115
0.251CysTyr: 0.251 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
8.087AspAla: 8.087 ± 0.843
0.627AspCys: 0.627 ± 0.228
5.203AspAsp: 5.203 ± 0.658
6.269AspGlu: 6.269 ± 0.687
1.693AspPhe: 1.693 ± 0.294
7.397AspGly: 7.397 ± 0.705
1.379AspHis: 1.379 ± 0.297
2.319AspIle: 2.319 ± 0.318
1.818AspLys: 1.818 ± 0.276
5.14AspLeu: 5.14 ± 0.673
1.191AspMet: 1.191 ± 0.238
1.943AspAsn: 1.943 ± 0.343
4.451AspPro: 4.451 ± 0.604
2.57AspGln: 2.57 ± 0.376
5.015AspArg: 5.015 ± 0.566
2.57AspSer: 2.57 ± 0.395
3.009AspThr: 3.009 ± 0.525
4.388AspVal: 4.388 ± 0.544
1.63AspTrp: 1.63 ± 0.316
1.693AspTyr: 1.693 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
6.645GluAla: 6.645 ± 0.69
0.502GluCys: 0.502 ± 0.172
3.134GluAsp: 3.134 ± 0.411
2.319GluGlu: 2.319 ± 0.458
2.257GluPhe: 2.257 ± 0.319
3.699GluGly: 3.699 ± 0.517
1.505GluHis: 1.505 ± 0.289
4.2GluIle: 4.2 ± 0.579
1.755GluLys: 1.755 ± 0.309
7.961GluLeu: 7.961 ± 0.933
1.442GluMet: 1.442 ± 0.282
1.567GluAsn: 1.567 ± 0.298
3.197GluPro: 3.197 ± 0.625
2.319GluGln: 2.319 ± 0.375
4.388GluArg: 4.388 ± 0.539
3.072GluSer: 3.072 ± 0.447
3.072GluThr: 3.072 ± 0.414
3.887GluVal: 3.887 ± 0.431
1.442GluTrp: 1.442 ± 0.241
1.755GluTyr: 1.755 ± 0.278
0.0GluXaa: 0.0 ± 0.0
Phe
2.633PheAla: 2.633 ± 0.424
0.376PheCys: 0.376 ± 0.153
1.881PheAsp: 1.881 ± 0.491
2.069PheGlu: 2.069 ± 0.395
0.439PhePhe: 0.439 ± 0.123
3.949PheGly: 3.949 ± 0.715
0.69PheHis: 0.69 ± 0.191
1.818PheIle: 1.818 ± 0.351
1.316PheLys: 1.316 ± 0.251
2.069PheLeu: 2.069 ± 0.331
0.376PheMet: 0.376 ± 0.154
0.69PheAsn: 0.69 ± 0.163
1.818PhePro: 1.818 ± 0.368
1.003PheGln: 1.003 ± 0.259
1.505PheArg: 1.505 ± 0.269
1.128PheSer: 1.128 ± 0.334
1.943PheThr: 1.943 ± 0.317
1.881PheVal: 1.881 ± 0.277
0.376PheTrp: 0.376 ± 0.147
0.94PheTyr: 0.94 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
9.152GlyAla: 9.152 ± 1.504
0.627GlyCys: 0.627 ± 0.208
5.893GlyAsp: 5.893 ± 0.444
4.764GlyGlu: 4.764 ± 0.578
2.946GlyPhe: 2.946 ± 0.628
10.218GlyGly: 10.218 ± 2.131
1.505GlyHis: 1.505 ± 0.293
3.009GlyIle: 3.009 ± 0.355
3.197GlyLys: 3.197 ± 0.397
7.711GlyLeu: 7.711 ± 0.847
1.63GlyMet: 1.63 ± 0.301
3.322GlyAsn: 3.322 ± 0.516
4.639GlyPro: 4.639 ± 0.5
3.573GlyGln: 3.573 ± 0.616
5.955GlyArg: 5.955 ± 0.49
4.89GlySer: 4.89 ± 0.77
6.331GlyThr: 6.331 ± 0.871
7.335GlyVal: 7.335 ± 0.699
1.63GlyTrp: 1.63 ± 0.357
2.946GlyTyr: 2.946 ± 0.523
0.0GlyXaa: 0.0 ± 0.0
His
1.818HisAla: 1.818 ± 0.31
0.251HisCys: 0.251 ± 0.105
1.442HisAsp: 1.442 ± 0.282
1.003HisGlu: 1.003 ± 0.341
0.376HisPhe: 0.376 ± 0.17
1.693HisGly: 1.693 ± 0.322
0.752HisHis: 0.752 ± 0.274
0.94HisIle: 0.94 ± 0.208
0.752HisLys: 0.752 ± 0.187
1.881HisLeu: 1.881 ± 0.397
0.313HisMet: 0.313 ± 0.136
0.502HisAsn: 0.502 ± 0.163
1.755HisPro: 1.755 ± 0.372
1.003HisGln: 1.003 ± 0.244
2.194HisArg: 2.194 ± 0.454
0.69HisSer: 0.69 ± 0.241
1.442HisThr: 1.442 ± 0.325
1.316HisVal: 1.316 ± 0.289
0.627HisTrp: 0.627 ± 0.192
0.94HisTyr: 0.94 ± 0.263
0.0HisXaa: 0.0 ± 0.0
Ile
6.081IleAla: 6.081 ± 0.389
0.439IleCys: 0.439 ± 0.169
4.137IleAsp: 4.137 ± 0.572
3.636IleGlu: 3.636 ± 0.459
0.815IlePhe: 0.815 ± 0.203
3.322IleGly: 3.322 ± 0.47
0.69IleHis: 0.69 ± 0.182
1.128IleIle: 1.128 ± 0.237
1.191IleLys: 1.191 ± 0.262
2.131IleLeu: 2.131 ± 0.326
0.502IleMet: 0.502 ± 0.255
1.191IleAsn: 1.191 ± 0.294
2.633IlePro: 2.633 ± 0.431
1.755IleGln: 1.755 ± 0.35
3.699IleArg: 3.699 ± 0.447
1.943IleSer: 1.943 ± 0.281
3.636IleThr: 3.636 ± 0.474
3.636IleVal: 3.636 ± 0.548
0.627IleTrp: 0.627 ± 0.187
0.94IleTyr: 0.94 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
3.761LysAla: 3.761 ± 0.497
0.502LysCys: 0.502 ± 0.176
1.567LysAsp: 1.567 ± 0.292
0.94LysGlu: 0.94 ± 0.292
1.066LysPhe: 1.066 ± 0.243
2.131LysGly: 2.131 ± 0.348
0.627LysHis: 0.627 ± 0.236
1.254LysIle: 1.254 ± 0.215
0.752LysLys: 0.752 ± 0.209
2.884LysLeu: 2.884 ± 0.47
0.502LysMet: 0.502 ± 0.157
0.815LysAsn: 0.815 ± 0.236
2.257LysPro: 2.257 ± 0.364
1.191LysGln: 1.191 ± 0.249
2.633LysArg: 2.633 ± 0.36
1.693LysSer: 1.693 ± 0.483
2.508LysThr: 2.508 ± 0.348
2.194LysVal: 2.194 ± 0.349
0.376LysTrp: 0.376 ± 0.136
0.878LysTyr: 0.878 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
10.782LeuAla: 10.782 ± 0.729
0.752LeuCys: 0.752 ± 0.244
6.081LeuAsp: 6.081 ± 0.57
3.322LeuGlu: 3.322 ± 0.339
2.257LeuPhe: 2.257 ± 0.321
8.087LeuGly: 8.087 ± 0.696
1.254LeuHis: 1.254 ± 0.26
4.764LeuIle: 4.764 ± 0.573
1.755LeuLys: 1.755 ± 0.394
7.46LeuLeu: 7.46 ± 0.632
1.442LeuMet: 1.442 ± 0.331
2.508LeuAsn: 2.508 ± 0.521
5.266LeuPro: 5.266 ± 0.424
2.758LeuGln: 2.758 ± 0.459
5.266LeuArg: 5.266 ± 0.585
3.385LeuSer: 3.385 ± 0.429
4.89LeuThr: 4.89 ± 0.46
4.702LeuVal: 4.702 ± 0.493
0.752LeuTrp: 0.752 ± 0.178
1.128LeuTyr: 1.128 ± 0.262
0.0LeuXaa: 0.0 ± 0.0
Met
3.322MetAla: 3.322 ± 0.403
0.063MetCys: 0.063 ± 0.053
1.191MetAsp: 1.191 ± 0.28
0.439MetGlu: 0.439 ± 0.152
0.439MetPhe: 0.439 ± 0.152
1.316MetGly: 1.316 ± 0.305
0.439MetHis: 0.439 ± 0.163
0.878MetIle: 0.878 ± 0.322
0.502MetLys: 0.502 ± 0.208
1.316MetLeu: 1.316 ± 0.317
0.313MetMet: 0.313 ± 0.153
0.878MetAsn: 0.878 ± 0.221
1.066MetPro: 1.066 ± 0.266
0.94MetGln: 0.94 ± 0.265
1.567MetArg: 1.567 ± 0.34
1.693MetSer: 1.693 ± 0.319
2.382MetThr: 2.382 ± 0.407
0.752MetVal: 0.752 ± 0.224
0.188MetTrp: 0.188 ± 0.114
0.063MetTyr: 0.063 ± 0.064
0.0MetXaa: 0.0 ± 0.0
Asn
3.573AsnAla: 3.573 ± 0.496
0.376AsnCys: 0.376 ± 0.15
2.069AsnAsp: 2.069 ± 0.371
1.505AsnGlu: 1.505 ± 0.299
0.376AsnPhe: 0.376 ± 0.149
3.448AsnGly: 3.448 ± 0.4
0.752AsnHis: 0.752 ± 0.303
1.254AsnIle: 1.254 ± 0.282
0.564AsnLys: 0.564 ± 0.194
1.943AsnLeu: 1.943 ± 0.45
0.125AsnMet: 0.125 ± 0.098
1.003AsnAsn: 1.003 ± 0.268
2.696AsnPro: 2.696 ± 0.416
1.003AsnGln: 1.003 ± 0.265
2.069AsnArg: 2.069 ± 0.418
1.818AsnSer: 1.818 ± 0.398
1.881AsnThr: 1.881 ± 0.334
1.818AsnVal: 1.818 ± 0.276
1.003AsnTrp: 1.003 ± 0.188
0.439AsnTyr: 0.439 ± 0.132
0.0AsnXaa: 0.0 ± 0.0
Pro
7.961ProAla: 7.961 ± 0.703
0.376ProCys: 0.376 ± 0.172
4.388ProAsp: 4.388 ± 0.519
4.451ProGlu: 4.451 ± 0.669
1.818ProPhe: 1.818 ± 0.339
5.955ProGly: 5.955 ± 0.603
0.752ProHis: 0.752 ± 0.196
2.194ProIle: 2.194 ± 0.338
2.069ProLys: 2.069 ± 0.446
4.576ProLeu: 4.576 ± 0.464
1.066ProMet: 1.066 ± 0.24
2.382ProAsn: 2.382 ± 0.446
4.137ProPro: 4.137 ± 0.74
2.57ProGln: 2.57 ± 0.446
4.075ProArg: 4.075 ± 0.587
2.696ProSer: 2.696 ± 0.377
3.197ProThr: 3.197 ± 0.469
5.015ProVal: 5.015 ± 0.515
1.442ProTrp: 1.442 ± 0.291
1.316ProTyr: 1.316 ± 0.257
0.0ProXaa: 0.0 ± 0.0
Gln
4.764GlnAla: 4.764 ± 0.643
0.376GlnCys: 0.376 ± 0.147
1.379GlnAsp: 1.379 ± 0.303
1.818GlnGlu: 1.818 ± 0.345
2.069GlnPhe: 2.069 ± 0.645
3.009GlnGly: 3.009 ± 0.54
0.69GlnHis: 0.69 ± 0.176
2.006GlnIle: 2.006 ± 0.302
0.878GlnLys: 0.878 ± 0.208
2.946GlnLeu: 2.946 ± 0.437
1.128GlnMet: 1.128 ± 0.247
0.69GlnAsn: 0.69 ± 0.204
2.382GlnPro: 2.382 ± 0.334
2.319GlnGln: 2.319 ± 0.389
2.946GlnArg: 2.946 ± 0.35
2.069GlnSer: 2.069 ± 0.404
1.755GlnThr: 1.755 ± 0.296
3.072GlnVal: 3.072 ± 0.464
0.94GlnTrp: 0.94 ± 0.222
0.69GlnTyr: 0.69 ± 0.166
0.0GlnXaa: 0.0 ± 0.0
Arg
8.087ArgAla: 8.087 ± 0.713
1.066ArgCys: 1.066 ± 0.32
5.14ArgAsp: 5.14 ± 0.587
3.636ArgGlu: 3.636 ± 0.551
2.069ArgPhe: 2.069 ± 0.308
5.14ArgGly: 5.14 ± 0.637
2.069ArgHis: 2.069 ± 0.393
3.26ArgIle: 3.26 ± 0.506
3.26ArgLys: 3.26 ± 0.373
5.078ArgLeu: 5.078 ± 0.591
2.445ArgMet: 2.445 ± 0.37
2.006ArgAsn: 2.006 ± 0.379
3.573ArgPro: 3.573 ± 0.461
2.194ArgGln: 2.194 ± 0.42
6.331ArgArg: 6.331 ± 0.949
3.134ArgSer: 3.134 ± 0.346
4.388ArgThr: 4.388 ± 0.47
4.764ArgVal: 4.764 ± 0.628
1.254ArgTrp: 1.254 ± 0.255
2.257ArgTyr: 2.257 ± 0.367
0.0ArgXaa: 0.0 ± 0.0
Ser
5.83SerAla: 5.83 ± 0.839
0.125SerCys: 0.125 ± 0.084
3.26SerAsp: 3.26 ± 0.398
2.633SerGlu: 2.633 ± 0.476
1.191SerPhe: 1.191 ± 0.267
5.517SerGly: 5.517 ± 0.91
1.191SerHis: 1.191 ± 0.264
1.818SerIle: 1.818 ± 0.317
1.442SerLys: 1.442 ± 0.305
3.26SerLeu: 3.26 ± 0.439
1.003SerMet: 1.003 ± 0.267
1.379SerAsn: 1.379 ± 0.254
3.009SerPro: 3.009 ± 0.478
1.505SerGln: 1.505 ± 0.297
2.696SerArg: 2.696 ± 0.361
2.319SerSer: 2.319 ± 0.366
3.636SerThr: 3.636 ± 0.516
3.636SerVal: 3.636 ± 0.429
1.003SerTrp: 1.003 ± 0.342
1.316SerTyr: 1.316 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
7.773ThrAla: 7.773 ± 0.832
0.627ThrCys: 0.627 ± 0.242
4.263ThrAsp: 4.263 ± 0.584
3.636ThrGlu: 3.636 ± 0.53
1.63ThrPhe: 1.63 ± 0.435
6.645ThrGly: 6.645 ± 0.858
1.505ThrHis: 1.505 ± 0.283
3.26ThrIle: 3.26 ± 0.453
2.131ThrLys: 2.131 ± 0.408
4.2ThrLeu: 4.2 ± 0.449
1.379ThrMet: 1.379 ± 0.33
1.63ThrAsn: 1.63 ± 0.285
4.952ThrPro: 4.952 ± 0.509
2.069ThrGln: 2.069 ± 0.324
4.012ThrArg: 4.012 ± 0.436
2.006ThrSer: 2.006 ± 0.316
3.448ThrThr: 3.448 ± 0.587
5.893ThrVal: 5.893 ± 0.592
1.505ThrTrp: 1.505 ± 0.348
1.254ThrTyr: 1.254 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
7.836ValAla: 7.836 ± 0.638
0.752ValCys: 0.752 ± 0.223
5.266ValAsp: 5.266 ± 0.847
5.14ValGlu: 5.14 ± 0.68
2.194ValPhe: 2.194 ± 0.318
5.14ValGly: 5.14 ± 0.61
1.755ValHis: 1.755 ± 0.342
2.758ValIle: 2.758 ± 0.347
1.943ValLys: 1.943 ± 0.437
4.514ValLeu: 4.514 ± 0.503
1.505ValMet: 1.505 ± 0.348
2.257ValAsn: 2.257 ± 0.357
4.514ValPro: 4.514 ± 0.484
2.006ValGln: 2.006 ± 0.379
4.514ValArg: 4.514 ± 0.603
3.511ValSer: 3.511 ± 0.503
5.893ValThr: 5.893 ± 0.538
5.83ValVal: 5.83 ± 0.78
1.379ValTrp: 1.379 ± 0.253
1.693ValTyr: 1.693 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
1.943TrpAla: 1.943 ± 0.294
0.439TrpCys: 0.439 ± 0.161
1.003TrpAsp: 1.003 ± 0.224
0.815TrpGlu: 0.815 ± 0.208
1.316TrpPhe: 1.316 ± 0.306
1.316TrpGly: 1.316 ± 0.278
0.313TrpHis: 0.313 ± 0.127
0.502TrpIle: 0.502 ± 0.171
0.439TrpLys: 0.439 ± 0.152
1.755TrpLeu: 1.755 ± 0.296
0.502TrpMet: 0.502 ± 0.178
0.878TrpAsn: 0.878 ± 0.192
1.191TrpPro: 1.191 ± 0.335
1.066TrpGln: 1.066 ± 0.233
1.254TrpArg: 1.254 ± 0.296
1.191TrpSer: 1.191 ± 0.245
1.066TrpThr: 1.066 ± 0.245
1.316TrpVal: 1.316 ± 0.293
0.69TrpTrp: 0.69 ± 0.191
0.439TrpTyr: 0.439 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.197TyrAla: 3.197 ± 0.367
0.251TyrCys: 0.251 ± 0.123
1.567TyrAsp: 1.567 ± 0.327
1.379TyrGlu: 1.379 ± 0.29
0.502TyrPhe: 0.502 ± 0.19
2.257TyrGly: 2.257 ± 0.416
0.627TyrHis: 0.627 ± 0.17
1.128TyrIle: 1.128 ± 0.268
0.376TyrLys: 0.376 ± 0.165
1.818TyrLeu: 1.818 ± 0.272
0.313TyrMet: 0.313 ± 0.195
0.752TyrAsn: 0.752 ± 0.26
1.316TyrPro: 1.316 ± 0.331
0.878TyrGln: 0.878 ± 0.269
1.693TyrArg: 1.693 ± 0.355
1.505TyrSer: 1.505 ± 0.243
2.069TyrThr: 2.069 ± 0.359
1.316TyrVal: 1.316 ± 0.326
0.502TyrTrp: 0.502 ± 0.172
0.439TyrTyr: 0.439 ± 0.134
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (15953 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski