Gordonia phage Angelique
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385DWD3|A0A385DWD3_9CAUD Membrane protein OS=Gordonia phage Angelique OX=2301691 GN=24 PE=4 SV=1
MM1 pKa = 6.88 STTRR5 pKa = 11.84 RR6 pKa = 11.84 LLALGAAALFLVAGCSSDD24 pKa = 4.56 DD25 pKa = 3.82 GSSSSTTTAAATATTTATSTKK46 pKa = 9.61 PRR48 pKa = 11.84 PTLGQLNTPYY58 pKa = 10.84 EE59 pKa = 4.12 LTDD62 pKa = 3.47 RR63 pKa = 11.84 SGGDD67 pKa = 3.13 QVKK70 pKa = 10.66 YY71 pKa = 10.3 SVTSIAVDD79 pKa = 4.18 GQCPDD84 pKa = 2.77 TYY86 pKa = 11.56 YY87 pKa = 11.49 NDD89 pKa = 3.56 VEE91 pKa = 4.39 NGHH94 pKa = 6.79 LLVVGLDD101 pKa = 3.55 VEE103 pKa = 5.15 TGADD107 pKa = 3.33 WDD109 pKa = 4.23 PEE111 pKa = 4.19 FGEE114 pKa = 4.28 MFRR117 pKa = 11.84 PRR119 pKa = 11.84 GGYY122 pKa = 5.88 WTIVGDD128 pKa = 3.96 NGVTEE133 pKa = 4.25 SGITSAASYY142 pKa = 10.86 GCADD146 pKa = 3.57 SQIPQTFDD154 pKa = 3.24 PDD156 pKa = 3.09 SRR158 pKa = 11.84 YY159 pKa = 9.99 QFSIAFDD166 pKa = 3.5 TRR168 pKa = 11.84 NASGMLQLRR177 pKa = 11.84 VGEE180 pKa = 4.39 STTRR184 pKa = 11.84 WEE186 pKa = 4.28 YY187 pKa = 11.54
Molecular weight: 19.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.154
IPC2_protein 4.228
IPC_protein 4.177
Toseland 3.973
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.088
Rodwell 4.012
Grimsley 3.884
Solomon 4.151
Lehninger 4.113
Nozaki 4.279
DTASelect 4.52
Thurlkill 4.012
EMBOSS 4.101
Sillero 4.304
Patrickios 3.16
IPC_peptide 4.151
IPC2_peptide 4.279
IPC2.peptide.svr19 4.223
Protein with the highest isoelectric point:
>tr|A0A385DTW5|A0A385DTW5_9CAUD Portal protein OS=Gordonia phage Angelique OX=2301691 GN=3 PE=4 SV=1
MM1 pKa = 7.71 PKK3 pKa = 10.5 GVFPRR8 pKa = 11.84 CEE10 pKa = 3.74 RR11 pKa = 11.84 CGSRR15 pKa = 11.84 VQQWTVRR22 pKa = 11.84 VCARR26 pKa = 11.84 CKK28 pKa = 9.84 LARR31 pKa = 11.84 NQKK34 pKa = 9.08 EE35 pKa = 4.31 VNQQ38 pKa = 3.88
Molecular weight: 4.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.288
IPC2_protein 9.282
IPC_protein 9.545
Toseland 10.613
ProMoST 10.116
Dawson 10.657
Bjellqvist 10.335
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.774
Lehninger 10.774
Nozaki 10.687
DTASelect 10.277
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.613
Patrickios 10.833
IPC_peptide 10.789
IPC2_peptide 9.882
IPC2.peptide.svr19 8.316
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
16435
38
1874
216.3
23.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.658 ± 0.646
0.919 ± 0.143
7.082 ± 0.309
5.397 ± 0.246
2.805 ± 0.124
8.75 ± 0.472
2.02 ± 0.229
4.527 ± 0.19
3.261 ± 0.212
7.752 ± 0.22
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.057 ± 0.147
3.207 ± 0.24
5.543 ± 0.336
3.803 ± 0.487
7.441 ± 0.397
5.78 ± 0.233
6.644 ± 0.282
7.113 ± 0.324
2.075 ± 0.184
2.166 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here