Hyunsoonleella pacifica

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Hyunsoonleella

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9FMP5|A0A4Q9FMP5_9FLAO Alpha-L-rhamnosidase OS=Hyunsoonleella pacifica OX=1080224 GN=EYD46_16395 PE=4 SV=1
MM1 pKa = 7.34IFKK4 pKa = 10.22IIKK7 pKa = 9.12KK8 pKa = 9.53YY9 pKa = 10.18NIGVILLLSMFLALISCYY27 pKa = 9.54DD28 pKa = 3.15TGYY31 pKa = 11.23EE32 pKa = 4.06EE33 pKa = 5.3FVPPTGNVNNIQPNTLFTTTTSADD57 pKa = 3.42DD58 pKa = 3.45NLRR61 pKa = 11.84IVFRR65 pKa = 11.84SYY67 pKa = 10.09STDD70 pKa = 2.93AVSYY74 pKa = 10.43LWDD77 pKa = 4.47FGDD80 pKa = 4.46GNTSTEE86 pKa = 3.87ANPNYY91 pKa = 10.06TYY93 pKa = 10.64ATGGLYY99 pKa = 10.37AVTLNTVSSDD109 pKa = 3.19GLEE112 pKa = 4.35ANASANVSPIFVDD125 pKa = 4.81FNFSAIDD132 pKa = 3.61SEE134 pKa = 5.02VTFEE138 pKa = 4.12NLTTGAEE145 pKa = 4.1SLVWDD150 pKa = 5.02FGDD153 pKa = 4.06GEE155 pKa = 4.53SVEE158 pKa = 4.19WSIEE162 pKa = 3.99DD163 pKa = 4.31SEE165 pKa = 4.87ADD167 pKa = 3.35PDD169 pKa = 4.25FSPVYY174 pKa = 10.08SYY176 pKa = 9.63KK177 pKa = 10.07TAEE180 pKa = 4.24TFQATLTATNYY191 pKa = 10.69LGVQVSITKK200 pKa = 10.4NIEE203 pKa = 3.75GLVLSTVPDD212 pKa = 3.71FTFDD216 pKa = 3.41VSSLEE221 pKa = 4.01VQFTDD226 pKa = 3.34TSILAVSHH234 pKa = 6.25SWDD237 pKa = 3.61FGDD240 pKa = 5.51GNTSTDD246 pKa = 3.1ASPLHH251 pKa = 6.47TYY253 pKa = 7.98ATAGTYY259 pKa = 10.53DD260 pKa = 3.5VTLTTTNAAGVSRR273 pKa = 11.84SITKK277 pKa = 8.83PVPVGGIEE285 pKa = 3.9PTFKK289 pKa = 11.02VIVQNSDD296 pKa = 3.97CNVHH300 pKa = 6.1TSNTGDD306 pKa = 4.3NADD309 pKa = 3.98AWDD312 pKa = 3.94MTPNSTVVDD321 pKa = 4.47DD322 pKa = 4.44NLGTIDD328 pKa = 3.64SPYY331 pKa = 9.76RR332 pKa = 11.84ALWRR336 pKa = 11.84NDD338 pKa = 3.21EE339 pKa = 4.19LNAYY343 pKa = 9.16IDD345 pKa = 3.64ATFCTNEE352 pKa = 4.16QPGSTSDD359 pKa = 3.53GNKK362 pKa = 9.87FGPDD366 pKa = 2.9AGGGRR371 pKa = 11.84GVKK374 pKa = 10.41LSNNCRR380 pKa = 11.84RR381 pKa = 11.84LYY383 pKa = 10.48QLVAVEE389 pKa = 4.63PGVEE393 pKa = 4.03YY394 pKa = 10.23TFTIDD399 pKa = 3.3TRR401 pKa = 11.84SEE403 pKa = 3.63AAGINTEE410 pKa = 4.26VFILNNEE417 pKa = 3.87ITTEE421 pKa = 3.96EE422 pKa = 4.72AINTPAALAINTDD435 pKa = 3.78GYY437 pKa = 12.02ALIDD441 pKa = 3.46NDD443 pKa = 3.99FNSSKK448 pKa = 10.79SSSTNDD454 pKa = 2.94TFTTTTLTFEE464 pKa = 4.3ASANIAVIYY473 pKa = 10.32VRR475 pKa = 11.84ALNAIDD481 pKa = 3.79SSNEE485 pKa = 3.66VFIDD489 pKa = 4.06NIDD492 pKa = 3.83IITPGFDD499 pKa = 2.85

Molecular weight: 53.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2JB50|A0A4V2JB50_9FLAO 3-phosphoglycerate dehydrogenase OS=Hyunsoonleella pacifica OX=1080224 GN=EYD46_03580 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.03TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3449

0

3449

1242761

22

3122

360.3

40.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.045 ± 0.041

0.778 ± 0.013

5.816 ± 0.036

6.38 ± 0.036

5.241 ± 0.033

6.281 ± 0.042

1.769 ± 0.019

8.105 ± 0.041

7.953 ± 0.068

9.161 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.978 ± 0.023

6.645 ± 0.049

3.292 ± 0.023

3.215 ± 0.022

3.342 ± 0.027

6.616 ± 0.035

5.985 ± 0.055

6.149 ± 0.028

1.081 ± 0.015

4.171 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski