Wenzhouxiangella sp. W260

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Wenzhouxiangellaceae; Wenzhouxiangella; unclassified Wenzhouxiangella

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N0TLE1|A0A5N0TLE1_9GAMM TolC family protein OS=Wenzhouxiangella sp. W260 OX=2613842 GN=F3N42_00925 PE=3 SV=1
MM1 pKa = 7.33TPTVVATASRR11 pKa = 11.84HH12 pKa = 4.91RR13 pKa = 11.84FSLALTSTLLLLAGPIEE30 pKa = 4.13AQVFINEE37 pKa = 3.83LHH39 pKa = 6.25YY40 pKa = 11.5DD41 pKa = 3.76NVSGDD46 pKa = 3.49SGEE49 pKa = 4.25MVEE52 pKa = 4.56LAGPAGTSLAGWTVALYY69 pKa = 10.57NGSASQLNVYY79 pKa = 8.45DD80 pKa = 4.58TINLSGTFADD90 pKa = 3.89QAGGMGTLAFNRR102 pKa = 11.84AGIQNGSPDD111 pKa = 3.6GLALVDD117 pKa = 3.91GSNNVVQFLSYY128 pKa = 10.79EE129 pKa = 4.41GVFTAASGPAAGMTSTDD146 pKa = 2.87IGVEE150 pKa = 4.16EE151 pKa = 5.02GSGTPVGHH159 pKa = 6.54SLQLVGAGLAYY170 pKa = 10.31DD171 pKa = 3.91DD172 pKa = 4.41FTWAEE177 pKa = 3.93AMAATPGQPNTGQDD191 pKa = 3.46FGGEE195 pKa = 3.92PSVPEE200 pKa = 4.2LLLSEE205 pKa = 5.16LVVTPTGGEE214 pKa = 3.99FIEE217 pKa = 4.19IHH219 pKa = 6.11NPGGSAVDD227 pKa = 3.98LSDD230 pKa = 5.18VYY232 pKa = 10.92LTDD235 pKa = 3.23ATFAGGNTYY244 pKa = 10.6YY245 pKa = 11.29YY246 pKa = 10.81NIVLGANAGGGGFSDD261 pKa = 3.95FHH263 pKa = 8.69ARR265 pKa = 11.84FPAGASIPAGGFQTVALAGSEE286 pKa = 4.31AFFAEE291 pKa = 4.72YY292 pKa = 10.2GVQPTYY298 pKa = 11.34EE299 pKa = 4.1LFEE302 pKa = 6.89DD303 pKa = 5.1GAADD307 pKa = 5.53AIPDD311 pKa = 3.66MRR313 pKa = 11.84EE314 pKa = 3.86ALPGSINAQGGLTNSGEE331 pKa = 4.53VVVLYY336 pKa = 10.36HH337 pKa = 6.7WDD339 pKa = 3.54EE340 pKa = 4.57SSDD343 pKa = 3.68LVADD347 pKa = 4.38LDD349 pKa = 4.13YY350 pKa = 11.4AVWGDD355 pKa = 3.27KK356 pKa = 11.17DD357 pKa = 3.7EE358 pKa = 5.72AVDD361 pKa = 3.68KK362 pKa = 11.11TGVALDD368 pKa = 4.58GPDD371 pKa = 3.57GDD373 pKa = 4.54TLVSEE378 pKa = 4.84YY379 pKa = 11.19AADD382 pKa = 3.94TPIASQDD389 pKa = 3.82VISGGAHH396 pKa = 6.41AFGEE400 pKa = 4.69SFTRR404 pKa = 11.84IDD406 pKa = 3.53FTEE409 pKa = 4.64GSEE412 pKa = 4.27TQSGGNGVNGADD424 pKa = 3.37EE425 pKa = 4.28TSEE428 pKa = 4.01NLSVTWASRR437 pKa = 11.84TPSPNSDD444 pKa = 3.88YY445 pKa = 11.31VPPTPDD451 pKa = 3.21LVITEE456 pKa = 4.12VMQNPAAVGDD466 pKa = 4.29SSGEE470 pKa = 3.59WFEE473 pKa = 3.7IHH475 pKa = 6.59NAGDD479 pKa = 3.31VDD481 pKa = 3.82VDD483 pKa = 3.68INGWTIADD491 pKa = 4.37LGSDD495 pKa = 3.4SHH497 pKa = 7.53VINAGGPLVVPAGGYY512 pKa = 8.05MVLGNNADD520 pKa = 3.69SGSNGGLVVDD530 pKa = 4.13YY531 pKa = 10.32AYY533 pKa = 11.23SGVFLANGDD542 pKa = 4.08DD543 pKa = 3.77EE544 pKa = 5.98LVLFDD549 pKa = 3.93AAGFEE554 pKa = 4.48VDD556 pKa = 3.55GVAWDD561 pKa = 4.85GGPVWPDD568 pKa = 3.01PTGASMALIDD578 pKa = 5.65LALDD582 pKa = 3.9NNDD585 pKa = 3.09GNNWCEE591 pKa = 4.0SQNPYY596 pKa = 11.1GDD598 pKa = 3.89GDD600 pKa = 3.74RR601 pKa = 11.84GTPGVANSCEE611 pKa = 3.9VLIPEE616 pKa = 5.44FGACGDD622 pKa = 3.84AATKK626 pKa = 9.92IHH628 pKa = 6.56AVQGNGLASPIAGSAGVIIEE648 pKa = 4.22GVVVGDD654 pKa = 3.5FQGGDD659 pKa = 3.14RR660 pKa = 11.84LNGFFVQEE668 pKa = 3.95EE669 pKa = 4.37SADD672 pKa = 3.74VDD674 pKa = 3.66GDD676 pKa = 3.86PATSEE681 pKa = 4.55GIFVYY686 pKa = 10.6DD687 pKa = 3.44EE688 pKa = 4.19NFGVDD693 pKa = 3.65VNVGDD698 pKa = 3.91VVRR701 pKa = 11.84VQGTVIEE708 pKa = 4.88FFDD711 pKa = 3.86LTEE714 pKa = 4.15ISPVINLVTCGTGTADD730 pKa = 3.53TQDD733 pKa = 3.14ILLPLAGPDD742 pKa = 3.24ALEE745 pKa = 4.24ALEE748 pKa = 4.35GMSVHH753 pKa = 7.03LPQTLFVTDD762 pKa = 3.68NFTLGRR768 pKa = 11.84YY769 pKa = 9.66GEE771 pKa = 4.25VGLAVGGPLDD781 pKa = 3.48IPTNVVAPGAAAQALQAQNDD801 pKa = 3.82LSRR804 pKa = 11.84IQLDD808 pKa = 4.14DD809 pKa = 3.85GSGLQNPVPVPPYY822 pKa = 9.31FAPDD826 pKa = 3.11GTLRR830 pKa = 11.84VGDD833 pKa = 3.73ATSAVTGVLGFGFGEE848 pKa = 4.4YY849 pKa = 9.7EE850 pKa = 3.74IHH852 pKa = 6.35PTDD855 pKa = 3.09VVAFSRR861 pKa = 11.84EE862 pKa = 3.79NEE864 pKa = 4.27RR865 pKa = 11.84PDD867 pKa = 4.1DD868 pKa = 4.45APDD871 pKa = 3.26VGNPLVTVAGFNVLNYY887 pKa = 7.55FTTLDD892 pKa = 4.43DD893 pKa = 3.81NTPVCGPSASMDD905 pKa = 3.22CRR907 pKa = 11.84GAEE910 pKa = 3.69NAFEE914 pKa = 4.44FEE916 pKa = 4.29RR917 pKa = 11.84QRR919 pKa = 11.84SKK921 pKa = 10.7IVAALSLLGADD932 pKa = 3.16VVGLVEE938 pKa = 4.27VEE940 pKa = 4.08NAAGDD945 pKa = 4.27GPVADD950 pKa = 5.03LVDD953 pKa = 5.33GINDD957 pKa = 3.22ILGADD962 pKa = 4.18TYY964 pKa = 11.71AYY966 pKa = 9.54IATGAIGTDD975 pKa = 4.44AIRR978 pKa = 11.84QALIYY983 pKa = 10.41KK984 pKa = 8.98PDD986 pKa = 3.28AVTPVGGFEE995 pKa = 4.46TLDD998 pKa = 3.77SSDD1001 pKa = 4.37DD1002 pKa = 3.57PAFIDD1007 pKa = 3.7TANRR1011 pKa = 11.84PALAQSFTDD1020 pKa = 4.19GSTGEE1025 pKa = 4.33VFTVAVNHH1033 pKa = 6.36LKK1035 pKa = 10.85SKK1037 pKa = 10.9GSDD1040 pKa = 3.4CDD1042 pKa = 3.77GLGDD1046 pKa = 4.84PDD1048 pKa = 4.01TGDD1051 pKa = 3.44GQGNCNLIRR1060 pKa = 11.84AAAASALATWLAADD1074 pKa = 3.8PTGSGSEE1081 pKa = 3.72RR1082 pKa = 11.84SLIIGDD1088 pKa = 4.15LNAYY1092 pKa = 9.83AKK1094 pKa = 9.95EE1095 pKa = 4.02DD1096 pKa = 4.57PIVVLKK1102 pKa = 10.93DD1103 pKa = 3.09AGFVDD1108 pKa = 5.13LVEE1111 pKa = 4.57VYY1113 pKa = 10.85NGVGYY1118 pKa = 11.02ADD1120 pKa = 3.66GAYY1123 pKa = 10.06SYY1125 pKa = 11.65GFGGQMGYY1133 pKa = 10.54LDD1135 pKa = 4.38HH1136 pKa = 7.3ALASAAFAADD1146 pKa = 3.5ASGATAWHH1154 pKa = 6.63INADD1158 pKa = 3.57EE1159 pKa = 4.33PAALDD1164 pKa = 4.14YY1165 pKa = 11.32NDD1167 pKa = 4.96HH1168 pKa = 5.99NQDD1171 pKa = 3.38VLFSPDD1177 pKa = 3.28PYY1179 pKa = 10.28RR1180 pKa = 11.84ASDD1183 pKa = 3.35HH1184 pKa = 6.7DD1185 pKa = 4.14AVVVGLFNDD1194 pKa = 3.71EE1195 pKa = 4.91DD1196 pKa = 5.36DD1197 pKa = 4.86DD1198 pKa = 5.1GVWDD1202 pKa = 5.21TIDD1205 pKa = 3.64ACPATVIPEE1214 pKa = 4.21TPPTRR1219 pKa = 11.84GLGNNRR1225 pKa = 11.84FALLDD1230 pKa = 4.1DD1231 pKa = 5.65DD1232 pKa = 5.75GVFDD1236 pKa = 3.88TTEE1239 pKa = 4.05GGGPGVSFTIHH1250 pKa = 6.38DD1251 pKa = 3.85TAGCSCEE1258 pKa = 4.12QIIAAADD1265 pKa = 3.88LGAGHH1270 pKa = 7.37AKK1272 pKa = 9.99FGCSIGEE1279 pKa = 3.74IRR1281 pKa = 11.84DD1282 pKa = 3.58WVSAQQ1287 pKa = 3.07

Molecular weight: 132.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N0T971|A0A5N0T971_9GAMM DUF481 domain-containing protein OS=Wenzhouxiangella sp. W260 OX=2613842 GN=F3N42_13780 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.1RR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.41GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.67GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3042

0

3042

1066961

25

2914

350.7

38.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.541 ± 0.064

0.868 ± 0.017

6.664 ± 0.038

6.089 ± 0.04

3.676 ± 0.029

8.662 ± 0.036

2.153 ± 0.021

4.506 ± 0.032

2.485 ± 0.034

10.199 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.02

2.888 ± 0.026

5.108 ± 0.032

3.405 ± 0.025

7.048 ± 0.048

5.326 ± 0.034

5.23 ± 0.04

7.569 ± 0.036

1.643 ± 0.022

2.506 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski