Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P12418|MU2_REOVD Microtubule-associated protein mu-2 OS=Reovirus type 3 (strain Dearing) OX=10886 GN=M1 PE=1 SV=1
MM1 pKa = 7.34 ANVWGVRR8 pKa = 11.84 LADD11 pKa = 3.82 SLSSPTIEE19 pKa = 4.01 TRR21 pKa = 11.84 TRR23 pKa = 11.84 QYY25 pKa = 10.47 TLHH28 pKa = 7.18 DD29 pKa = 4.26 LCSDD33 pKa = 3.92 LDD35 pKa = 4.12 ANPGRR40 pKa = 11.84 EE41 pKa = 3.64 PWKK44 pKa = 9.54 PLRR47 pKa = 11.84 NQRR50 pKa = 11.84 TNNIVAVQLFRR61 pKa = 11.84 PLQGLVLDD69 pKa = 4.01 TQLYY73 pKa = 8.21 GFPGAFDD80 pKa = 3.07 DD81 pKa = 3.81 WEE83 pKa = 4.65 RR84 pKa = 11.84 FMRR87 pKa = 11.84 EE88 pKa = 3.38 KK89 pKa = 11.01 LRR91 pKa = 11.84 VLKK94 pKa = 11.15 YY95 pKa = 9.5 EE96 pKa = 3.91 VLRR99 pKa = 11.84 IYY101 pKa = 10.23 PISNYY106 pKa = 10.13 SNEE109 pKa = 4.08 HH110 pKa = 5.13 VNVFVANALVGAFLSNQAFYY130 pKa = 11.48 DD131 pKa = 4.14 LLPLLIINDD140 pKa = 3.8 TMIGDD145 pKa = 4.58 LLGTGASLSQFFQSHH160 pKa = 6.04 GDD162 pKa = 3.68 VLEE165 pKa = 4.24 VAAGRR170 pKa = 11.84 KK171 pKa = 7.8 YY172 pKa = 10.94 LQMEE176 pKa = 4.56 NYY178 pKa = 10.61 SNDD181 pKa = 3.83 DD182 pKa = 3.58 DD183 pKa = 5.86 DD184 pKa = 5.56 PPLFAKK190 pKa = 10.55 DD191 pKa = 3.38 LSDD194 pKa = 3.66 YY195 pKa = 11.6 AKK197 pKa = 10.8 AFYY200 pKa = 10.69 SDD202 pKa = 3.4 TYY204 pKa = 10.92 EE205 pKa = 3.91 VLDD208 pKa = 3.77 RR209 pKa = 11.84 FFWTHH214 pKa = 6.75 DD215 pKa = 3.09 SSAGVLVHH223 pKa = 6.38 YY224 pKa = 8.23 DD225 pKa = 3.06 KK226 pKa = 10.18 PTNGHH231 pKa = 6.61 HH232 pKa = 6.2 YY233 pKa = 11.06 LLGTLTQMVSAPPYY247 pKa = 10.06 IINATDD253 pKa = 3.2 AMLLEE258 pKa = 4.74 SCLEE262 pKa = 3.89 QFSANVRR269 pKa = 11.84 ARR271 pKa = 11.84 PAQPVTRR278 pKa = 11.84 LDD280 pKa = 3.2 QCYY283 pKa = 9.51 HH284 pKa = 5.99 LRR286 pKa = 11.84 WGAQYY291 pKa = 10.87 VGEE294 pKa = 4.72 DD295 pKa = 3.3 SLTYY299 pKa = 10.4 RR300 pKa = 11.84 LGVLSLLATNGYY312 pKa = 9.17 QLARR316 pKa = 11.84 PIPRR320 pKa = 11.84 QLTNRR325 pKa = 11.84 WLSSFVSQIMSDD337 pKa = 3.81 GVNEE341 pKa = 4.18 TPLWPQEE348 pKa = 4.03 RR349 pKa = 11.84 YY350 pKa = 8.46 VQIAYY355 pKa = 9.43 DD356 pKa = 3.79 SPSVVDD362 pKa = 4.59 GATQYY367 pKa = 11.67 GYY369 pKa = 10.63 VRR371 pKa = 11.84 KK372 pKa = 9.02 NQLRR376 pKa = 11.84 LGMRR380 pKa = 11.84 ISALQSLSDD389 pKa = 3.84 TPSPVQWLPQYY400 pKa = 10.55 TIDD403 pKa = 4.28 QAAMDD408 pKa = 4.7 EE409 pKa = 4.25 GDD411 pKa = 4.05 LMVSRR416 pKa = 11.84 LTQLPLRR423 pKa = 11.84 PDD425 pKa = 3.52 YY426 pKa = 11.63 GNIWVGDD433 pKa = 3.82 ALSYY437 pKa = 10.3 YY438 pKa = 10.06 VDD440 pKa = 3.67 YY441 pKa = 11.11 NRR443 pKa = 11.84 SHH445 pKa = 6.66 RR446 pKa = 11.84 VVLSSEE452 pKa = 4.47 LPQLPDD458 pKa = 2.96 TYY460 pKa = 11.3 FDD462 pKa = 3.83 GDD464 pKa = 3.65 EE465 pKa = 4.0 QYY467 pKa = 11.5 GRR469 pKa = 11.84 SLFSLARR476 pKa = 11.84 KK477 pKa = 9.61 IGDD480 pKa = 3.21 RR481 pKa = 11.84 SLVKK485 pKa = 9.91 DD486 pKa = 3.47 TAVLKK491 pKa = 10.42 HH492 pKa = 6.56 AYY494 pKa = 8.89 QAIDD498 pKa = 3.52 PNTGKK503 pKa = 10.6 EE504 pKa = 4.1 YY505 pKa = 10.63 LRR507 pKa = 11.84 SRR509 pKa = 11.84 QSVAYY514 pKa = 8.95 FGASAGHH521 pKa = 6.32 SGADD525 pKa = 3.12 QPLVIEE531 pKa = 4.54 PWIQGKK537 pKa = 9.89 ISGVPPPSSVRR548 pKa = 11.84 QFGYY552 pKa = 10.76 DD553 pKa = 3.29 VARR556 pKa = 11.84 GAIVDD561 pKa = 4.35 LARR564 pKa = 11.84 PFPSGDD570 pKa = 3.41 YY571 pKa = 10.22 QFVYY575 pKa = 10.9 SDD577 pKa = 3.15 VDD579 pKa = 3.69 QVVDD583 pKa = 3.69 GHH585 pKa = 7.25 DD586 pKa = 4.11 DD587 pKa = 3.62 LSISSGLVEE596 pKa = 4.94 SLLSSCMHH604 pKa = 6.35 ATAPGGSFVVKK615 pKa = 10.23 INFPTRR621 pKa = 11.84 PVWHH625 pKa = 6.69 YY626 pKa = 10.51 IEE628 pKa = 5.3 QKK630 pKa = 9.72 ILPNITSYY638 pKa = 10.97 MLIKK642 pKa = 10.53 PFVTNNVEE650 pKa = 4.06 LFFVAFGVHH659 pKa = 4.71 QHH661 pKa = 6.6 SSLTWTSGVYY671 pKa = 10.02 FFLVDD676 pKa = 3.09 HH677 pKa = 7.04 FYY679 pKa = 10.67 RR680 pKa = 11.84 YY681 pKa = 7.46 EE682 pKa = 3.98 TLSTISRR689 pKa = 11.84 QLPSFGYY696 pKa = 10.34 VDD698 pKa = 4.85 DD699 pKa = 5.1 GSSVTGIEE707 pKa = 4.39 TISIEE712 pKa = 4.16 NPGFSNMTQAARR724 pKa = 11.84 IGISGLCANVGNARR738 pKa = 11.84 KK739 pKa = 9.98 SIAIYY744 pKa = 8.9 EE745 pKa = 4.3 SHH747 pKa = 6.82 GARR750 pKa = 11.84 VLTITSRR757 pKa = 11.84 RR758 pKa = 11.84 SPASARR764 pKa = 11.84 RR765 pKa = 11.84 KK766 pKa = 9.65 SRR768 pKa = 11.84 LRR770 pKa = 11.84 YY771 pKa = 9.57 LPLIDD776 pKa = 4.18 PRR778 pKa = 11.84 SLEE781 pKa = 4.04 VQARR785 pKa = 11.84 TILPADD791 pKa = 3.38 PVLFEE796 pKa = 4.41 NVSGASPHH804 pKa = 5.75 VCLTMMYY811 pKa = 9.72 NFEE814 pKa = 4.24 VSSAVYY820 pKa = 10.61 DD821 pKa = 3.73 GDD823 pKa = 3.94 VVLDD827 pKa = 4.5 LGTGPEE833 pKa = 3.96 AKK835 pKa = 9.76 ILEE838 pKa = 5.29 LIPATSPVTCVDD850 pKa = 3.28 IRR852 pKa = 11.84 PTAQPSGCWNVRR864 pKa = 11.84 TTFLEE869 pKa = 3.91 LDD871 pKa = 3.83 YY872 pKa = 11.51 LSDD875 pKa = 3.26 GWITGVRR882 pKa = 11.84 GDD884 pKa = 4.46 IVTCMLSLGAAAAGKK899 pKa = 10.91 SMTFDD904 pKa = 3.32 AAFQQLIKK912 pKa = 10.78 VLSKK916 pKa = 9.66 STANVVLVQVNCPTDD931 pKa = 3.4 VVRR934 pKa = 11.84 SIKK937 pKa = 10.54 GYY939 pKa = 10.91 LEE941 pKa = 3.89 IDD943 pKa = 3.83 STNKK947 pKa = 10.08 RR948 pKa = 11.84 YY949 pKa = 10.15 RR950 pKa = 11.84 FPKK953 pKa = 10.01 FGRR956 pKa = 11.84 DD957 pKa = 3.29 EE958 pKa = 4.96 PYY960 pKa = 10.91 SDD962 pKa = 3.58 MDD964 pKa = 4.02 ALEE967 pKa = 4.72 KK968 pKa = 10.16 ICRR971 pKa = 11.84 TAWPNCSITWVPLSYY986 pKa = 10.89 DD987 pKa = 3.71 LRR989 pKa = 11.84 WTRR992 pKa = 11.84 LALLEE997 pKa = 4.23 STTLSSASIRR1007 pKa = 11.84 IAEE1010 pKa = 4.22 LMYY1013 pKa = 10.6 KK1014 pKa = 9.97 YY1015 pKa = 9.76 MPIMRR1020 pKa = 11.84 IDD1022 pKa = 3.18 IHH1024 pKa = 6.13 GLPMEE1029 pKa = 4.29 KK1030 pKa = 9.6 RR1031 pKa = 11.84 GNFIVGQNCSLVIPGFNAQDD1051 pKa = 3.46 VFNCYY1056 pKa = 9.73 FNSALAFSTEE1066 pKa = 4.13 DD1067 pKa = 3.22 VNAAMIPQVSAQFDD1081 pKa = 3.63 ATKK1084 pKa = 11.06 GEE1086 pKa = 4.08 WTLDD1090 pKa = 3.42 MVFSDD1095 pKa = 3.23 AGIYY1099 pKa = 8.99 TMQALVGSNANPVSLGSFVVDD1120 pKa = 4.37 SPDD1123 pKa = 4.22 VDD1125 pKa = 3.82 ITDD1128 pKa = 3.6 AWPAQLDD1135 pKa = 3.67 FTIAGTDD1142 pKa = 2.91 VDD1144 pKa = 3.83 ITVNPYY1150 pKa = 8.82 YY1151 pKa = 10.96 RR1152 pKa = 11.84 LMTFVRR1158 pKa = 11.84 IDD1160 pKa = 3.81 GQWQIANPDD1169 pKa = 3.1 KK1170 pKa = 11.12 FQFFSSASGTLVMNVKK1186 pKa = 10.45 LDD1188 pKa = 3.41 IADD1191 pKa = 4.19 KK1192 pKa = 10.68 YY1193 pKa = 10.02 LLYY1196 pKa = 10.57 YY1197 pKa = 9.88 IRR1199 pKa = 11.84 DD1200 pKa = 3.64 VQSRR1204 pKa = 11.84 DD1205 pKa = 3.04 VGFYY1209 pKa = 9.9 IQHH1212 pKa = 7.14 PLQLLNTITLPTNEE1226 pKa = 5.26 DD1227 pKa = 3.81 LFLSAPDD1234 pKa = 3.44 MRR1236 pKa = 11.84 EE1237 pKa = 3.61 WAVKK1241 pKa = 10.18 EE1242 pKa = 4.15 SGNTICILNSQGFVLPQDD1260 pKa = 3.21 WDD1262 pKa = 3.8 VLTDD1266 pKa = 4.23 TISWSPSIPTYY1277 pKa = 10.24 IVPPGDD1283 pKa = 3.52 YY1284 pKa = 10.21 TLTPLL1289 pKa = 4.79
Molecular weight: 143.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.864
IPC2_protein 4.952
IPC_protein 4.952
Toseland 4.863
ProMoST 5.118
Dawson 4.978
Bjellqvist 5.105
Wikipedia 4.889
Rodwell 4.851
Grimsley 4.787
Solomon 4.978
Lehninger 4.927
Nozaki 5.092
DTASelect 5.334
Thurlkill 4.889
EMBOSS 4.927
Sillero 5.143
Patrickios 3.567
IPC_peptide 4.978
IPC2_peptide 5.143
IPC2.peptide.svr19 5.064
Protein with the highest isoelectric point:
>sp|P03526|SIGNS_REOVD Protein sigma-NS OS=Reovirus type 3 (strain Dearing) OX=10886 GN=S3 PE=2 SV=1
MM1 pKa = 7.31 ARR3 pKa = 11.84 AAFLFKK9 pKa = 10.33 TVGFGGLQNVPINDD23 pKa = 3.83 EE24 pKa = 4.26 LSSHH28 pKa = 6.8 LLRR31 pKa = 11.84 AGNSPWQLTQFLDD44 pKa = 4.17 WISLGRR50 pKa = 11.84 GLATSALVPTAGSRR64 pKa = 11.84 YY65 pKa = 8.74 YY66 pKa = 11.13 QMSCLLSGTLQIPFRR81 pKa = 11.84 PNHH84 pKa = 5.6 RR85 pKa = 11.84 WGDD88 pKa = 3.35 IRR90 pKa = 11.84 FLRR93 pKa = 11.84 LVWSAPTLDD102 pKa = 4.12 GLVVAPPQVLAQPALQAQADD122 pKa = 4.12 RR123 pKa = 11.84 VYY125 pKa = 11.29 DD126 pKa = 3.87 CDD128 pKa = 5.2 DD129 pKa = 3.71 YY130 pKa = 11.78 PFLARR135 pKa = 11.84 DD136 pKa = 3.34 PRR138 pKa = 11.84 FKK140 pKa = 10.89 HH141 pKa = 6.07 RR142 pKa = 11.84 VYY144 pKa = 10.5 QQLSAVTLLNLTGFGPISYY163 pKa = 10.03 VRR165 pKa = 11.84 VDD167 pKa = 3.06 EE168 pKa = 5.2 DD169 pKa = 3.21 MWSGDD174 pKa = 3.63 VNQLLMNYY182 pKa = 9.49 FGHH185 pKa = 6.38 TFAEE189 pKa = 4.2 IAYY192 pKa = 6.69 TLCQASANRR201 pKa = 11.84 PWEE204 pKa = 3.74 YY205 pKa = 10.93 DD206 pKa = 2.89 GTYY209 pKa = 11.24 ARR211 pKa = 11.84 MTQIVLSLFWLSYY224 pKa = 10.67 VGVIHH229 pKa = 6.07 QQNTYY234 pKa = 8.17 RR235 pKa = 11.84 TFYY238 pKa = 9.35 FQCNRR243 pKa = 11.84 RR244 pKa = 11.84 GDD246 pKa = 3.7 AAEE249 pKa = 4.12 VWILSCSLNHH259 pKa = 6.59 SAQIRR264 pKa = 11.84 PGNRR268 pKa = 11.84 SLFVMPTSPDD278 pKa = 2.57 WNMDD282 pKa = 3.01 VNLILSSTLTGCLCSGSQLPLIDD305 pKa = 4.33 NNSVPAVSRR314 pKa = 11.84 NIHH317 pKa = 5.35 GWTGRR322 pKa = 11.84 AGNQLHH328 pKa = 6.48 GFQVRR333 pKa = 11.84 RR334 pKa = 11.84 MVTEE338 pKa = 4.08 FCDD341 pKa = 3.06 RR342 pKa = 11.84 LRR344 pKa = 11.84 RR345 pKa = 11.84 DD346 pKa = 3.17 GVMTQAQQNQVEE358 pKa = 4.24 ALADD362 pKa = 3.24 QTQQFKK368 pKa = 10.57 RR369 pKa = 11.84 DD370 pKa = 3.67 KK371 pKa = 10.52 LEE373 pKa = 3.53 TWARR377 pKa = 11.84 EE378 pKa = 3.64 DD379 pKa = 3.39 DD380 pKa = 4.48 QYY382 pKa = 12.13 NQAHH386 pKa = 6.94 PNSTMFRR393 pKa = 11.84 TKK395 pKa = 10.55 PFTNAQWGRR404 pKa = 11.84 GNTGATSAAIAALII418 pKa = 4.13
Molecular weight: 47.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.5
IPC2_protein 7.644
IPC_protein 7.541
Toseland 7.132
ProMoST 8.053
Dawson 8.17
Bjellqvist 8.609
Wikipedia 8.083
Rodwell 8.17
Grimsley 7.176
Solomon 8.258
Lehninger 8.273
Nozaki 8.756
DTASelect 8.302
Thurlkill 8.331
EMBOSS 8.375
Sillero 8.668
Patrickios 4.126
IPC_peptide 8.244
IPC2_peptide 8.068
IPC2.peptide.svr19 7.98
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
1
11
8281
365
1289
752.8
83.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.006 ± 0.375
1.352 ± 0.152
6.461 ± 0.296
4.371 ± 0.292
3.502 ± 0.177
5.482 ± 0.369
2.005 ± 0.222
5.664 ± 0.219
3.768 ± 0.36
9.576 ± 0.353
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.236 ± 0.282
3.997 ± 0.363
5.482 ± 0.41
4.685 ± 0.347
5.651 ± 0.242
8.369 ± 0.304
6.135 ± 0.407
7.366 ± 0.354
1.473 ± 0.176
3.417 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here