Prevotella aff. ruminicola Tc2-24

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0MCE8|A0A1I0MCE8_9BACT Uncharacterized protein OS=Prevotella aff. ruminicola Tc2-24 OX=81582 GN=SAMN04487850_0537 PE=4 SV=1
MM1 pKa = 7.38NATIVIYY8 pKa = 10.41GSSTGTCEE16 pKa = 5.07AIAEE20 pKa = 4.44KK21 pKa = 10.28IASKK25 pKa = 10.53LGCEE29 pKa = 4.05AVNVQDD35 pKa = 3.8LTADD39 pKa = 3.07IVSNNQNLILGTSTWGAGEE58 pKa = 4.8LQDD61 pKa = 5.13DD62 pKa = 4.63WYY64 pKa = 10.78DD65 pKa = 3.71GLKK68 pKa = 9.16TLQGADD74 pKa = 3.75LNGKK78 pKa = 7.62TIAIFGCGDD87 pKa = 3.54CQSYY91 pKa = 11.48GDD93 pKa = 4.01TFVGGMGEE101 pKa = 4.66LYY103 pKa = 10.87NGIKK107 pKa = 10.52ASGARR112 pKa = 11.84FIGSVEE118 pKa = 4.08TDD120 pKa = 3.15GYY122 pKa = 9.92TYY124 pKa = 10.97DD125 pKa = 4.43DD126 pKa = 4.23SEE128 pKa = 4.27AVIDD132 pKa = 4.37GKK134 pKa = 11.16FIGLPLDD141 pKa = 5.91DD142 pKa = 5.12INEE145 pKa = 4.27DD146 pKa = 3.7DD147 pKa = 4.0KK148 pKa = 11.68TDD150 pKa = 3.28ARR152 pKa = 11.84IEE154 pKa = 3.96AWVAAITPDD163 pKa = 3.34LL164 pKa = 4.25

Molecular weight: 17.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0LWU9|A0A1I0LWU9_9BACT Thioredoxin-like OS=Prevotella aff. ruminicola Tc2-24 OX=81582 GN=SAMN04487850_0023 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2446

0

2446

896847

29

2473

366.7

41.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.224 ± 0.046

1.356 ± 0.021

5.922 ± 0.034

6.261 ± 0.049

4.347 ± 0.029

6.989 ± 0.045

2.159 ± 0.024

6.659 ± 0.035

6.059 ± 0.044

8.975 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.024

4.615 ± 0.039

3.699 ± 0.025

3.825 ± 0.033

4.995 ± 0.036

5.821 ± 0.037

5.695 ± 0.038

6.71 ± 0.033

1.349 ± 0.021

4.343 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski