Amino acid dipepetide frequency for Prevotella aff. ruminicola Tc2-24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.788AlaAla: 5.788 ± 0.113	1.086AlaCys: 1.086 ± 0.038	4.669AlaAsp: 4.669 ± 0.074	4.69AlaGlu: 4.69 ± 0.092	3.17AlaPhe: 3.17 ± 0.071	4.861AlaGly: 4.861 ± 0.087	1.388AlaHis: 1.388 ± 0.04	5.018AlaIle: 5.018 ± 0.08	4.295AlaLys: 4.295 ± 0.079	6.758AlaLeu: 6.758 ± 0.103	2.236AlaMet: 2.236 ± 0.055	2.959AlaAsn: 2.959 ± 0.066	2.265AlaPro: 2.265 ± 0.059	3.092AlaGln: 3.092 ± 0.066	3.269AlaArg: 3.269 ± 0.065	4.089AlaSer: 4.089 ± 0.079	3.843AlaThr: 3.843 ± 0.076	4.98AlaVal: 4.98 ± 0.083	0.853AlaTrp: 0.853 ± 0.03	2.929AlaTyr: 2.929 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.963CysAla: 0.963 ± 0.035	0.245CysCys: 0.245 ± 0.018	0.736CysAsp: 0.736 ± 0.032	0.789CysGlu: 0.789 ± 0.027	0.597CysPhe: 0.597 ± 0.027	1.177CysGly: 1.177 ± 0.04	0.4CysHis: 0.4 ± 0.023	0.886CysIle: 0.886 ± 0.035	0.66CysLys: 0.66 ± 0.029	1.211CysLeu: 1.211 ± 0.038	0.346CysMet: 0.346 ± 0.022	0.578CysAsn: 0.578 ± 0.027	0.56CysPro: 0.56 ± 0.026	0.467CysGln: 0.467 ± 0.024	0.675CysArg: 0.675 ± 0.025	0.803CysSer: 0.803 ± 0.039	0.707CysThr: 0.707 ± 0.033	0.952CysVal: 0.952 ± 0.036	0.19CysTrp: 0.19 ± 0.015	0.615CysTyr: 0.615 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.231AspAla: 4.231 ± 0.071	0.763AspCys: 0.763 ± 0.031	3.511AspAsp: 3.511 ± 0.072	4.252AspGlu: 4.252 ± 0.079	3.002AspPhe: 3.002 ± 0.06	4.624AspGly: 4.624 ± 0.088	1.264AspHis: 1.264 ± 0.04	4.271AspIle: 4.271 ± 0.073	3.517AspLys: 3.517 ± 0.072	4.682AspLeu: 4.682 ± 0.078	1.748AspMet: 1.748 ± 0.041	2.652AspAsn: 2.652 ± 0.055	2.091AspPro: 2.091 ± 0.045	1.65AspGln: 1.65 ± 0.042	2.804AspArg: 2.804 ± 0.062	3.24AspSer: 3.24 ± 0.058	2.93AspThr: 2.93 ± 0.065	3.93AspVal: 3.93 ± 0.063	0.946AspTrp: 0.946 ± 0.036	3.115AspTyr: 3.115 ± 0.071	0.0AspXaa: 0.0 ± 0.0
Glu	4.972GluAla: 4.972 ± 0.086	0.687GluCys: 0.687 ± 0.032	3.264GluAsp: 3.264 ± 0.065	4.554GluGlu: 4.554 ± 0.094	2.199GluPhe: 2.199 ± 0.053	4.204GluGly: 4.204 ± 0.069	1.404GluHis: 1.404 ± 0.043	4.042GluIle: 4.042 ± 0.072	4.507GluLys: 4.507 ± 0.081	5.536GluLeu: 5.536 ± 0.084	2.075GluMet: 2.075 ± 0.052	3.114GluAsn: 3.114 ± 0.06	1.84GluPro: 1.84 ± 0.048	2.921GluGln: 2.921 ± 0.062	3.571GluArg: 3.571 ± 0.071	2.892GluSer: 2.892 ± 0.056	3.361GluThr: 3.361 ± 0.076	4.158GluVal: 4.158 ± 0.069	0.859GluTrp: 0.859 ± 0.029	2.449GluTyr: 2.449 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	2.92PheAla: 2.92 ± 0.068	0.836PheCys: 0.836 ± 0.035	2.866PheAsp: 2.866 ± 0.054	2.423PheGlu: 2.423 ± 0.061	2.139PhePhe: 2.139 ± 0.064	3.377PheGly: 3.377 ± 0.066	0.922PheHis: 0.922 ± 0.032	2.752PheIle: 2.752 ± 0.073	2.142PheLys: 2.142 ± 0.046	3.819PheLeu: 3.819 ± 0.085	1.227PheMet: 1.227 ± 0.042	2.003PheAsn: 2.003 ± 0.047	1.648PhePro: 1.648 ± 0.043	1.208PheGln: 1.208 ± 0.036	2.103PheArg: 2.103 ± 0.056	3.094PheSer: 3.094 ± 0.061	2.521PheThr: 2.521 ± 0.052	3.037PheVal: 3.037 ± 0.066	0.627PheTrp: 0.627 ± 0.027	1.804PheTyr: 1.804 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.631GlyAla: 4.631 ± 0.093	1.105GlyCys: 1.105 ± 0.04	3.952GlyAsp: 3.952 ± 0.065	4.095GlyGlu: 4.095 ± 0.066	3.109GlyPhe: 3.109 ± 0.061	5.055GlyGly: 5.055 ± 0.098	1.622GlyHis: 1.622 ± 0.044	5.232GlyIle: 5.232 ± 0.084	4.702GlyLys: 4.702 ± 0.087	6.061GlyLeu: 6.061 ± 0.093	2.168GlyMet: 2.168 ± 0.049	3.145GlyAsn: 3.145 ± 0.073	1.502GlyPro: 1.502 ± 0.041	2.502GlyGln: 2.502 ± 0.06	3.387GlyArg: 3.387 ± 0.075	3.953GlySer: 3.953 ± 0.067	4.239GlyThr: 4.239 ± 0.079	4.927GlyVal: 4.927 ± 0.079	1.093GlyTrp: 1.093 ± 0.042	3.41GlyTyr: 3.41 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	1.375HisAla: 1.375 ± 0.042	0.331HisCys: 0.331 ± 0.019	1.285HisAsp: 1.285 ± 0.039	1.2HisGlu: 1.2 ± 0.04	1.095HisPhe: 1.095 ± 0.037	1.503HisGly: 1.503 ± 0.042	0.688HisHis: 0.688 ± 0.037	1.572HisIle: 1.572 ± 0.042	0.908HisLys: 0.908 ± 0.036	2.073HisLeu: 2.073 ± 0.047	0.416HisMet: 0.416 ± 0.022	0.915HisAsn: 0.915 ± 0.036	1.122HisPro: 1.122 ± 0.043	0.943HisGln: 0.943 ± 0.032	1.137HisArg: 1.137 ± 0.034	1.119HisSer: 1.119 ± 0.035	1.153HisThr: 1.153 ± 0.039	1.402HisVal: 1.402 ± 0.042	0.304HisTrp: 0.304 ± 0.019	1.053HisTyr: 1.053 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.09IleAla: 5.09 ± 0.093	1.014IleCys: 1.014 ± 0.038	4.488IleAsp: 4.488 ± 0.075	4.203IleGlu: 4.203 ± 0.076	2.488IlePhe: 2.488 ± 0.062	4.815IleGly: 4.815 ± 0.09	1.385IleHis: 1.385 ± 0.042	4.46IleIle: 4.46 ± 0.093	3.642IleLys: 3.642 ± 0.076	5.736IleLeu: 5.736 ± 0.102	1.549IleMet: 1.549 ± 0.044	3.06IleAsn: 3.06 ± 0.067	3.114IlePro: 3.114 ± 0.075	2.246IleGln: 2.246 ± 0.053	3.523IleArg: 3.523 ± 0.071	4.15IleSer: 4.15 ± 0.064	3.857IleThr: 3.857 ± 0.069	4.506IleVal: 4.506 ± 0.087	0.734IleTrp: 0.734 ± 0.029	2.537IleTyr: 2.537 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	4.822LysAla: 4.822 ± 0.081	0.525LysCys: 0.525 ± 0.027	3.697LysAsp: 3.697 ± 0.07	4.69LysGlu: 4.69 ± 0.085	1.84LysPhe: 1.84 ± 0.044	4.218LysGly: 4.218 ± 0.076	1.152LysHis: 1.152 ± 0.036	3.725LysIle: 3.725 ± 0.078	4.559LysLys: 4.559 ± 0.082	4.46LysLeu: 4.46 ± 0.078	2.163LysMet: 2.163 ± 0.059	3.085LysAsn: 3.085 ± 0.068	2.087LysPro: 2.087 ± 0.043	2.314LysGln: 2.314 ± 0.052	3.066LysArg: 3.066 ± 0.061	3.15LysSer: 3.15 ± 0.062	3.604LysThr: 3.604 ± 0.068	4.11LysVal: 4.11 ± 0.067	0.735LysTrp: 0.735 ± 0.023	2.582LysTyr: 2.582 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	6.323LeuAla: 6.323 ± 0.092	1.442LeuCys: 1.442 ± 0.045	4.762LeuAsp: 4.762 ± 0.078	4.637LeuGlu: 4.637 ± 0.083	4.281LeuPhe: 4.281 ± 0.088	5.848LeuGly: 5.848 ± 0.099	1.88LeuHis: 1.88 ± 0.048	5.214LeuIle: 5.214 ± 0.098	5.483LeuLys: 5.483 ± 0.082	8.641LeuLeu: 8.641 ± 0.153	2.822LeuMet: 2.822 ± 0.063	4.192LeuAsn: 4.192 ± 0.076	3.839LeuPro: 3.839 ± 0.071	3.416LeuGln: 3.416 ± 0.056	4.691LeuArg: 4.691 ± 0.078	6.441LeuSer: 6.441 ± 0.106	5.706LeuThr: 5.706 ± 0.081	5.378LeuVal: 5.378 ± 0.081	1.135LeuTrp: 1.135 ± 0.037	3.614LeuTyr: 3.614 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	2.446MetAla: 2.446 ± 0.058	0.29MetCys: 0.29 ± 0.019	1.557MetAsp: 1.557 ± 0.045	1.79MetGlu: 1.79 ± 0.045	1.022MetPhe: 1.022 ± 0.035	2.075MetGly: 2.075 ± 0.05	0.543MetHis: 0.543 ± 0.028	1.745MetIle: 1.745 ± 0.046	2.53MetLys: 2.53 ± 0.053	2.745MetLeu: 2.745 ± 0.058	1.111MetMet: 1.111 ± 0.036	1.487MetAsn: 1.487 ± 0.043	1.315MetPro: 1.315 ± 0.043	1.148MetGln: 1.148 ± 0.041	1.609MetArg: 1.609 ± 0.041	1.559MetSer: 1.559 ± 0.04	1.943MetThr: 1.943 ± 0.042	1.927MetVal: 1.927 ± 0.045	0.307MetTrp: 0.307 ± 0.02	0.832MetTyr: 0.832 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.346AsnAla: 3.346 ± 0.069	0.544AsnCys: 0.544 ± 0.025	2.752AsnAsp: 2.752 ± 0.058	2.682AsnGlu: 2.682 ± 0.049	1.893AsnPhe: 1.893 ± 0.047	3.801AsnGly: 3.801 ± 0.08	1.053AsnHis: 1.053 ± 0.037	3.387AsnIle: 3.387 ± 0.061	2.604AsnLys: 2.604 ± 0.057	3.81AsnLeu: 3.81 ± 0.072	1.212AsnMet: 1.212 ± 0.037	2.217AsnAsn: 2.217 ± 0.053	2.282AsnPro: 2.282 ± 0.055	1.58AsnGln: 1.58 ± 0.048	2.268AsnArg: 2.268 ± 0.051	2.483AsnSer: 2.483 ± 0.07	2.521AsnThr: 2.521 ± 0.062	2.926AsnVal: 2.926 ± 0.059	0.623AsnTrp: 0.623 ± 0.026	2.166AsnTyr: 2.166 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	2.555ProAla: 2.555 ± 0.057	0.389ProCys: 0.389 ± 0.021	2.641ProAsp: 2.641 ± 0.041	3.121ProGlu: 3.121 ± 0.059	1.712ProPhe: 1.712 ± 0.049	2.252ProGly: 2.252 ± 0.05	0.784ProHis: 0.784 ± 0.034	2.202ProIle: 2.202 ± 0.051	1.984ProLys: 1.984 ± 0.046	3.313ProLeu: 3.313 ± 0.069	1.103ProMet: 1.103 ± 0.029	1.622ProAsn: 1.622 ± 0.048	0.74ProPro: 0.74 ± 0.032	1.669ProGln: 1.669 ± 0.049	1.442ProArg: 1.442 ± 0.041	2.146ProSer: 2.146 ± 0.047	2.056ProThr: 2.056 ± 0.047	2.947ProVal: 2.947 ± 0.056	0.501ProTrp: 0.501 ± 0.023	1.809ProTyr: 1.809 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.697GlnAla: 2.697 ± 0.066	0.323GlnCys: 0.323 ± 0.021	1.764GlnAsp: 1.764 ± 0.049	2.473GlnGlu: 2.473 ± 0.058	1.568GlnPhe: 1.568 ± 0.042	2.332GlnGly: 2.332 ± 0.055	0.847GlnHis: 0.847 ± 0.029	2.464GlnIle: 2.464 ± 0.048	2.592GlnLys: 2.592 ± 0.057	3.756GlnLeu: 3.756 ± 0.069	1.355GlnMet: 1.355 ± 0.042	1.731GlnAsn: 1.731 ± 0.048	1.432GlnPro: 1.432 ± 0.045	2.058GlnGln: 2.058 ± 0.054	2.048GlnArg: 2.048 ± 0.048	1.984GlnSer: 1.984 ± 0.044	2.326GlnThr: 2.326 ± 0.054	2.344GlnVal: 2.344 ± 0.05	0.594GlnTrp: 0.594 ± 0.026	1.564GlnTyr: 1.564 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	2.892ArgAla: 2.892 ± 0.072	0.59ArgCys: 0.59 ± 0.029	2.62ArgAsp: 2.62 ± 0.058	3.229ArgGlu: 3.229 ± 0.065	2.314ArgPhe: 2.314 ± 0.048	2.709ArgGly: 2.709 ± 0.061	1.27ArgHis: 1.27 ± 0.035	3.562ArgIle: 3.562 ± 0.064	3.111ArgLys: 3.111 ± 0.054	5.176ArgLeu: 5.176 ± 0.089	1.676ArgMet: 1.676 ± 0.042	2.35ArgAsn: 2.35 ± 0.051	1.742ArgPro: 1.742 ± 0.048	2.602ArgGln: 2.602 ± 0.056	2.936ArgArg: 2.936 ± 0.07	2.52ArgSer: 2.52 ± 0.056	2.461ArgThr: 2.461 ± 0.053	2.87ArgVal: 2.87 ± 0.059	0.802ArgTrp: 0.802 ± 0.031	2.521ArgTyr: 2.521 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.013SerAla: 4.013 ± 0.077	0.859SerCys: 0.859 ± 0.032	3.462SerAsp: 3.462 ± 0.062	3.268SerGlu: 3.268 ± 0.061	2.94SerPhe: 2.94 ± 0.059	4.188SerGly: 4.188 ± 0.077	1.309SerHis: 1.309 ± 0.038	3.972SerIle: 3.972 ± 0.086	3.118SerLys: 3.118 ± 0.06	5.672SerLeu: 5.672 ± 0.103	1.622SerMet: 1.622 ± 0.043	2.434SerAsn: 2.434 ± 0.063	2.207SerPro: 2.207 ± 0.044	2.156SerGln: 2.156 ± 0.052	2.64SerArg: 2.64 ± 0.057	3.663SerSer: 3.663 ± 0.071	3.099SerThr: 3.099 ± 0.064	4.082SerVal: 4.082 ± 0.069	0.845SerTrp: 0.845 ± 0.036	2.658SerTyr: 2.658 ± 0.069	0.0SerXaa: 0.0 ± 0.0
Thr	4.332ThrAla: 4.332 ± 0.072	0.692ThrCys: 0.692 ± 0.029	3.603ThrAsp: 3.603 ± 0.064	3.207ThrGlu: 3.207 ± 0.068	2.677ThrPhe: 2.677 ± 0.051	4.234ThrGly: 4.234 ± 0.08	1.141ThrHis: 1.141 ± 0.04	4.111ThrIle: 4.111 ± 0.076	2.895ThrLys: 2.895 ± 0.057	5.322ThrLeu: 5.322 ± 0.092	1.514ThrMet: 1.514 ± 0.044	2.362ThrAsn: 2.362 ± 0.057	2.53ThrPro: 2.53 ± 0.056	1.822ThrGln: 1.822 ± 0.049	2.403ThrArg: 2.403 ± 0.057	3.373ThrSer: 3.373 ± 0.069	3.41ThrThr: 3.41 ± 0.072	4.22ThrVal: 4.22 ± 0.07	0.702ThrTrp: 0.702 ± 0.026	2.401ThrTyr: 2.401 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.952ValAla: 4.952 ± 0.088	1.102ValCys: 1.102 ± 0.032	4.051ValAsp: 4.051 ± 0.072	4.058ValGlu: 4.058 ± 0.069	2.94ValPhe: 2.94 ± 0.058	4.496ValGly: 4.496 ± 0.08	1.112ValHis: 1.112 ± 0.032	4.489ValIle: 4.489 ± 0.09	4.177ValLys: 4.177 ± 0.074	5.851ValLeu: 5.851 ± 0.091	2.024ValMet: 2.024 ± 0.049	3.24ValAsn: 3.24 ± 0.059	2.5ValPro: 2.5 ± 0.049	1.966ValGln: 1.966 ± 0.051	3.295ValArg: 3.295 ± 0.069	4.386ValSer: 4.386 ± 0.074	3.976ValThr: 3.976 ± 0.074	4.964ValVal: 4.964 ± 0.081	0.835ValTrp: 0.835 ± 0.035	2.686ValTyr: 2.686 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.854TrpAla: 0.854 ± 0.033	0.176TrpCys: 0.176 ± 0.015	0.766TrpAsp: 0.766 ± 0.033	0.747TrpGlu: 0.747 ± 0.031	0.569TrpPhe: 0.569 ± 0.027	1.029TrpGly: 1.029 ± 0.037	0.366TrpHis: 0.366 ± 0.021	0.781TrpIle: 0.781 ± 0.03	0.814TrpLys: 0.814 ± 0.027	1.384TrpLeu: 1.384 ± 0.039	0.454TrpMet: 0.454 ± 0.025	0.727TrpAsn: 0.727 ± 0.033	0.356TrpPro: 0.356 ± 0.022	0.681TrpGln: 0.681 ± 0.027	0.723TrpArg: 0.723 ± 0.031	0.753TrpSer: 0.753 ± 0.033	0.762TrpThr: 0.762 ± 0.032	0.756TrpVal: 0.756 ± 0.034	0.221TrpTrp: 0.221 ± 0.018	0.572TrpTyr: 0.572 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.034TyrAla: 3.034 ± 0.06	0.559TyrCys: 0.559 ± 0.025	2.779TyrAsp: 2.779 ± 0.068	2.501TyrGlu: 2.501 ± 0.053	1.914TyrPhe: 1.914 ± 0.049	3.091TyrGly: 3.091 ± 0.067	1.064TyrHis: 1.064 ± 0.041	2.708TyrIle: 2.708 ± 0.059	2.348TyrLys: 2.348 ± 0.054	3.74TyrLeu: 3.74 ± 0.074	1.161TyrMet: 1.161 ± 0.037	2.277TyrAsn: 2.277 ± 0.06	1.817TyrPro: 1.817 ± 0.052	1.81TyrGln: 1.81 ± 0.051	2.363TyrArg: 2.363 ± 0.056	2.408TyrSer: 2.408 ± 0.064	2.476TyrThr: 2.476 ± 0.058	2.683TyrVal: 2.683 ± 0.052	0.584TyrTrp: 0.584 ± 0.028	2.113TyrTyr: 2.113 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2446 proteins (896847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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