Bacillus phage vB_BceM-HSE3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3EFM3|A0A2Z3EFM3_9CAUD Uncharacterized protein OS=Bacillus phage vB_BceM-HSE3 OX=2170705 GN=HSE3_gp013 PE=4 SV=1
MM1 pKa = 6.6NTSVQLTEE9 pKa = 4.33NGVEE13 pKa = 4.09VQVTRR18 pKa = 11.84KK19 pKa = 8.11ITIGSTFSISPSQAFMGIDD38 pKa = 3.22AGSVEE43 pKa = 4.76VIDD46 pKa = 3.35ICKK49 pKa = 10.59YY50 pKa = 10.67EE51 pKa = 4.25DD52 pKa = 3.54LQEE55 pKa = 4.14EE56 pKa = 4.45AKK58 pKa = 10.58SDD60 pKa = 3.27ADD62 pKa = 3.83AYY64 pKa = 11.26LSTLEE69 pKa = 4.23EE70 pKa = 5.09FEE72 pKa = 5.91DD73 pKa = 4.68EE74 pKa = 4.37YY75 pKa = 11.73NQEE78 pKa = 4.75KK79 pKa = 10.29SSAWIVARR87 pKa = 11.84YY88 pKa = 9.89LDD90 pKa = 4.04TEE92 pKa = 4.1QFGTDD97 pKa = 3.01KK98 pKa = 11.05LAFPLHH104 pKa = 6.01TFVDD108 pKa = 4.86HH109 pKa = 7.46ISAFF113 pKa = 3.64

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3EDP1|A0A2Z3EDP1_9CAUD Baseplate hub subunit OS=Bacillus phage vB_BceM-HSE3 OX=2170705 GN=HSE3_gp074 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.41RR3 pKa = 11.84FLQFTLAITMSISIYY18 pKa = 8.87TFLVISTINVTSILCSLIAMVVALILLRR46 pKa = 11.84KK47 pKa = 8.79YY48 pKa = 10.73

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

144

0

144

37827

26

1294

262.7

29.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.316 ± 0.195

0.859 ± 0.077

6.326 ± 0.154

6.982 ± 0.306

3.73 ± 0.131

6.395 ± 0.326

1.739 ± 0.069

6.897 ± 0.185

7.251 ± 0.187

8.597 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.145

5.61 ± 0.203

3.696 ± 0.153

3.844 ± 0.194

4.037 ± 0.144

7.706 ± 0.237

6.382 ± 0.199

6.717 ± 0.169

1.06 ± 0.062

4.462 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski