Amino acid dipepetide frequency for Bacillus phage vB_BceM-HSE3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.666AlaAla: 1.666 ± 0.378
0.555AlaCys: 0.555 ± 0.115
3.463AlaAsp: 3.463 ± 0.34
3.595AlaGlu: 3.595 ± 0.301
1.851AlaPhe: 1.851 ± 0.259
3.886AlaGly: 3.886 ± 0.514
0.74AlaHis: 0.74 ± 0.127
3.966AlaIle: 3.966 ± 0.283
3.992AlaLys: 3.992 ± 0.331
4.864AlaLeu: 4.864 ± 0.35
1.454AlaMet: 1.454 ± 0.224
2.934AlaAsn: 2.934 ± 0.312
1.692AlaPro: 1.692 ± 0.203
2.855AlaGln: 2.855 ± 0.291
2.406AlaArg: 2.406 ± 0.226
3.595AlaSer: 3.595 ± 0.332
3.939AlaThr: 3.939 ± 0.331
2.961AlaVal: 2.961 ± 0.247
0.37AlaTrp: 0.37 ± 0.106
2.379AlaTyr: 2.379 ± 0.271
0.0AlaXaa: 0.0 ± 0.0
Cys
0.397CysAla: 0.397 ± 0.095
0.079CysCys: 0.079 ± 0.045
0.344CysAsp: 0.344 ± 0.105
0.661CysGlu: 0.661 ± 0.151
0.397CysPhe: 0.397 ± 0.104
0.449CysGly: 0.449 ± 0.131
0.106CysHis: 0.106 ± 0.045
0.687CysIle: 0.687 ± 0.135
0.82CysLys: 0.82 ± 0.147
0.687CysLeu: 0.687 ± 0.159
0.211CysMet: 0.211 ± 0.078
0.476CysAsn: 0.476 ± 0.099
0.37CysPro: 0.37 ± 0.115
0.264CysGln: 0.264 ± 0.075
0.291CysArg: 0.291 ± 0.101
0.608CysSer: 0.608 ± 0.165
0.767CysThr: 0.767 ± 0.156
0.449CysVal: 0.449 ± 0.116
0.159CysTrp: 0.159 ± 0.065
0.37CysTyr: 0.37 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
3.04AspAla: 3.04 ± 0.247
0.582AspCys: 0.582 ± 0.15
3.278AspAsp: 3.278 ± 0.252
4.521AspGlu: 4.521 ± 0.425
2.855AspPhe: 2.855 ± 0.26
4.203AspGly: 4.203 ± 0.452
0.925AspHis: 0.925 ± 0.181
4.283AspIle: 4.283 ± 0.385
5.076AspLys: 5.076 ± 0.386
6.266AspLeu: 6.266 ± 0.414
1.718AspMet: 1.718 ± 0.21
3.992AspAsn: 3.992 ± 0.432
2.512AspPro: 2.512 ± 0.254
2.459AspGln: 2.459 ± 0.239
2.247AspArg: 2.247 ± 0.218
4.203AspSer: 4.203 ± 0.337
3.728AspThr: 3.728 ± 0.294
3.648AspVal: 3.648 ± 0.367
0.608AspTrp: 0.608 ± 0.133
3.12AspTyr: 3.12 ± 0.246
0.0AspXaa: 0.0 ± 0.0
Glu
3.437GluAla: 3.437 ± 0.309
0.476GluCys: 0.476 ± 0.126
4.071GluAsp: 4.071 ± 0.43
6.398GluGlu: 6.398 ± 0.694
3.384GluPhe: 3.384 ± 0.362
4.626GluGly: 4.626 ± 0.294
1.031GluHis: 1.031 ± 0.146
4.838GluIle: 4.838 ± 0.349
3.543GluLys: 3.543 ± 0.314
7.64GluLeu: 7.64 ± 0.5
2.326GluMet: 2.326 ± 0.251
2.934GluAsn: 2.934 ± 0.27
2.036GluPro: 2.036 ± 0.277
2.379GluGln: 2.379 ± 0.266
2.3GluArg: 2.3 ± 0.288
5.525GluSer: 5.525 ± 0.472
2.934GluThr: 2.934 ± 0.297
6.107GluVal: 6.107 ± 0.437
0.925GluTrp: 0.925 ± 0.163
2.882GluTyr: 2.882 ± 0.275
0.0GluXaa: 0.0 ± 0.0
Phe
1.56PheAla: 1.56 ± 0.196
0.291PheCys: 0.291 ± 0.087
2.934PheAsp: 2.934 ± 0.277
2.723PheGlu: 2.723 ± 0.203
1.11PhePhe: 1.11 ± 0.181
2.538PheGly: 2.538 ± 0.259
0.608PheHis: 0.608 ± 0.118
2.697PheIle: 2.697 ± 0.294
3.172PheLys: 3.172 ± 0.279
3.12PheLeu: 3.12 ± 0.315
1.11PheMet: 1.11 ± 0.196
2.485PheAsn: 2.485 ± 0.289
1.216PhePro: 1.216 ± 0.196
1.243PheGln: 1.243 ± 0.161
1.322PheArg: 1.322 ± 0.187
2.934PheSer: 2.934 ± 0.313
2.459PheThr: 2.459 ± 0.261
2.009PheVal: 2.009 ± 0.207
0.37PheTrp: 0.37 ± 0.101
1.401PheTyr: 1.401 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
3.463GlyAla: 3.463 ± 0.513
0.661GlyCys: 0.661 ± 0.144
3.331GlyAsp: 3.331 ± 0.26
3.463GlyGlu: 3.463 ± 0.328
2.459GlyPhe: 2.459 ± 0.241
5.234GlyGly: 5.234 ± 1.169
0.925GlyHis: 0.925 ± 0.151
4.759GlyIle: 4.759 ± 0.317
4.626GlyLys: 4.626 ± 0.487
4.521GlyLeu: 4.521 ± 0.398
1.507GlyMet: 1.507 ± 0.207
3.569GlyAsn: 3.569 ± 0.373
1.084GlyPro: 1.084 ± 0.175
2.379GlyGln: 2.379 ± 0.463
2.829GlyArg: 2.829 ± 0.278
5.261GlySer: 5.261 ± 0.516
5.605GlyThr: 5.605 ± 0.492
4.732GlyVal: 4.732 ± 0.367
0.714GlyTrp: 0.714 ± 0.149
2.829GlyTyr: 2.829 ± 0.214
0.0GlyXaa: 0.0 ± 0.0
His
0.793HisAla: 0.793 ± 0.128
0.317HisCys: 0.317 ± 0.092
1.269HisAsp: 1.269 ± 0.18
1.031HisGlu: 1.031 ± 0.175
0.74HisPhe: 0.74 ± 0.154
1.057HisGly: 1.057 ± 0.153
0.476HisHis: 0.476 ± 0.109
1.216HisIle: 1.216 ± 0.174
1.269HisLys: 1.269 ± 0.185
1.428HisLeu: 1.428 ± 0.196
0.211HisMet: 0.211 ± 0.08
1.057HisAsn: 1.057 ± 0.16
0.978HisPro: 0.978 ± 0.139
0.767HisGln: 0.767 ± 0.127
0.661HisArg: 0.661 ± 0.142
1.057HisSer: 1.057 ± 0.174
1.031HisThr: 1.031 ± 0.159
1.005HisVal: 1.005 ± 0.185
0.211HisTrp: 0.211 ± 0.066
0.82HisTyr: 0.82 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
4.203IleAla: 4.203 ± 0.319
0.476IleCys: 0.476 ± 0.115
4.891IleAsp: 4.891 ± 0.366
5.367IleGlu: 5.367 ± 0.405
1.877IlePhe: 1.877 ± 0.236
3.754IleGly: 3.754 ± 0.36
1.243IleHis: 1.243 ± 0.175
4.389IleIle: 4.389 ± 0.407
5.684IleLys: 5.684 ± 0.393
4.97IleLeu: 4.97 ± 0.418
1.401IleMet: 1.401 ± 0.179
3.939IleAsn: 3.939 ± 0.376
3.331IlePro: 3.331 ± 0.289
2.723IleGln: 2.723 ± 0.259
3.12IleArg: 3.12 ± 0.324
5.446IleSer: 5.446 ± 0.442
4.917IleThr: 4.917 ± 0.527
4.045IleVal: 4.045 ± 0.416
0.555IleTrp: 0.555 ± 0.113
2.644IleTyr: 2.644 ± 0.256
0.0IleXaa: 0.0 ± 0.0
Lys
4.389LysAla: 4.389 ± 0.347
0.687LysCys: 0.687 ± 0.14
5.446LysAsp: 5.446 ± 0.397
4.626LysGlu: 4.626 ± 0.489
3.49LysPhe: 3.49 ± 0.286
4.917LysGly: 4.917 ± 0.383
1.137LysHis: 1.137 ± 0.183
4.6LysIle: 4.6 ± 0.325
4.97LysLys: 4.97 ± 0.38
7.297LysLeu: 7.297 ± 0.474
1.507LysMet: 1.507 ± 0.209
2.908LysAsn: 2.908 ± 0.258
2.379LysPro: 2.379 ± 0.286
1.93LysGln: 1.93 ± 0.217
2.564LysArg: 2.564 ± 0.315
5.79LysSer: 5.79 ± 0.42
3.807LysThr: 3.807 ± 0.321
5.895LysVal: 5.895 ± 0.42
0.793LysTrp: 0.793 ± 0.172
3.384LysTyr: 3.384 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
5.023LeuAla: 5.023 ± 0.332
0.714LeuCys: 0.714 ± 0.129
5.922LeuAsp: 5.922 ± 0.363
6.636LeuGlu: 6.636 ± 0.482
2.538LeuPhe: 2.538 ± 0.252
4.547LeuGly: 4.547 ± 0.337
1.956LeuHis: 1.956 ± 0.221
6.001LeuIle: 6.001 ± 0.451
7.138LeuLys: 7.138 ± 0.519
6.503LeuLeu: 6.503 ± 0.457
1.956LeuMet: 1.956 ± 0.228
4.468LeuAsn: 4.468 ± 0.357
3.331LeuPro: 3.331 ± 0.289
2.987LeuGln: 2.987 ± 0.255
4.045LeuArg: 4.045 ± 0.374
6.794LeuSer: 6.794 ± 0.481
5.367LeuThr: 5.367 ± 0.362
5.869LeuVal: 5.869 ± 0.337
0.714LeuTrp: 0.714 ± 0.132
3.463LeuTyr: 3.463 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
1.322MetAla: 1.322 ± 0.211
0.053MetCys: 0.053 ± 0.04
1.745MetAsp: 1.745 ± 0.266
1.692MetGlu: 1.692 ± 0.228
0.793MetPhe: 0.793 ± 0.138
1.057MetGly: 1.057 ± 0.228
0.476MetHis: 0.476 ± 0.122
1.877MetIle: 1.877 ± 0.225
1.903MetLys: 1.903 ± 0.284
1.586MetLeu: 1.586 ± 0.241
0.687MetMet: 0.687 ± 0.126
1.666MetAsn: 1.666 ± 0.19
1.005MetPro: 1.005 ± 0.178
0.555MetGln: 0.555 ± 0.151
0.793MetArg: 0.793 ± 0.164
2.036MetSer: 2.036 ± 0.232
1.93MetThr: 1.93 ± 0.226
1.295MetVal: 1.295 ± 0.19
0.344MetTrp: 0.344 ± 0.094
1.11MetTyr: 1.11 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
2.3AsnAla: 2.3 ± 0.294
0.793AsnCys: 0.793 ± 0.17
2.908AsnAsp: 2.908 ± 0.281
3.067AsnGlu: 3.067 ± 0.278
1.692AsnPhe: 1.692 ± 0.226
3.543AsnGly: 3.543 ± 0.28
0.899AsnHis: 0.899 ± 0.153
4.124AsnIle: 4.124 ± 0.368
3.78AsnLys: 3.78 ± 0.305
5.155AsnLeu: 5.155 ± 0.357
1.428AsnMet: 1.428 ± 0.199
3.543AsnAsn: 3.543 ± 0.372
2.644AsnPro: 2.644 ± 0.368
3.199AsnGln: 3.199 ± 0.505
1.824AsnArg: 1.824 ± 0.255
5.446AsnSer: 5.446 ± 0.478
3.648AsnThr: 3.648 ± 0.419
3.067AsnVal: 3.067 ± 0.334
0.529AsnTrp: 0.529 ± 0.113
2.512AsnTyr: 2.512 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
2.274ProAla: 2.274 ± 0.275
0.132ProCys: 0.132 ± 0.06
2.961ProAsp: 2.961 ± 0.258
3.648ProGlu: 3.648 ± 0.339
1.084ProPhe: 1.084 ± 0.163
1.718ProGly: 1.718 ± 0.222
0.582ProHis: 0.582 ± 0.127
2.326ProIle: 2.326 ± 0.263
2.247ProLys: 2.247 ± 0.248
2.802ProLeu: 2.802 ± 0.261
0.661ProMet: 0.661 ± 0.116
2.379ProAsn: 2.379 ± 0.307
1.507ProPro: 1.507 ± 0.267
1.348ProGln: 1.348 ± 0.27
1.454ProArg: 1.454 ± 0.219
2.749ProSer: 2.749 ± 0.285
2.723ProThr: 2.723 ± 0.306
2.512ProVal: 2.512 ± 0.24
0.291ProTrp: 0.291 ± 0.106
1.56ProTyr: 1.56 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
1.956GlnAla: 1.956 ± 0.247
0.344GlnCys: 0.344 ± 0.096
2.485GlnAsp: 2.485 ± 0.248
3.067GlnGlu: 3.067 ± 0.362
2.115GlnPhe: 2.115 ± 0.242
3.172GlnGly: 3.172 ± 0.623
0.397GlnHis: 0.397 ± 0.092
2.089GlnIle: 2.089 ± 0.218
1.454GlnLys: 1.454 ± 0.213
4.045GlnLeu: 4.045 ± 0.318
0.793GlnMet: 0.793 ± 0.138
1.666GlnAsn: 1.666 ± 0.236
1.56GlnPro: 1.56 ± 0.349
1.745GlnGln: 1.745 ± 0.382
1.348GlnArg: 1.348 ± 0.204
2.882GlnSer: 2.882 ± 0.295
1.48GlnThr: 1.48 ± 0.217
3.384GlnVal: 3.384 ± 0.319
0.529GlnTrp: 0.529 ± 0.129
1.93GlnTyr: 1.93 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
2.274ArgAla: 2.274 ± 0.224
0.344ArgCys: 0.344 ± 0.102
2.432ArgAsp: 2.432 ± 0.186
2.062ArgGlu: 2.062 ± 0.284
1.824ArgPhe: 1.824 ± 0.262
2.432ArgGly: 2.432 ± 0.23
0.555ArgHis: 0.555 ± 0.129
2.961ArgIle: 2.961 ± 0.272
3.305ArgLys: 3.305 ± 0.233
3.648ArgLeu: 3.648 ± 0.313
1.19ArgMet: 1.19 ± 0.184
2.141ArgAsn: 2.141 ± 0.269
1.11ArgPro: 1.11 ± 0.182
1.216ArgGln: 1.216 ± 0.203
1.851ArgArg: 1.851 ± 0.235
2.882ArgSer: 2.882 ± 0.325
2.564ArgThr: 2.564 ± 0.244
2.908ArgVal: 2.908 ± 0.299
0.687ArgTrp: 0.687 ± 0.166
1.983ArgTyr: 1.983 ± 0.264
0.0ArgXaa: 0.0 ± 0.0
Ser
4.441SerAla: 4.441 ± 0.398
0.634SerCys: 0.634 ± 0.14
4.732SerAsp: 4.732 ± 0.354
4.891SerGlu: 4.891 ± 0.358
2.459SerPhe: 2.459 ± 0.248
5.393SerGly: 5.393 ± 0.424
1.56SerHis: 1.56 ± 0.225
4.706SerIle: 4.706 ± 0.397
6.371SerLys: 6.371 ± 0.434
5.737SerLeu: 5.737 ± 0.38
1.666SerMet: 1.666 ± 0.227
4.626SerAsn: 4.626 ± 0.525
2.802SerPro: 2.802 ± 0.257
3.278SerGln: 3.278 ± 0.347
3.067SerArg: 3.067 ± 0.309
6.477SerSer: 6.477 ± 0.507
5.155SerThr: 5.155 ± 0.372
4.812SerVal: 4.812 ± 0.27
0.925SerTrp: 0.925 ± 0.161
3.331SerTyr: 3.331 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
3.41ThrAla: 3.41 ± 0.412
0.423ThrCys: 0.423 ± 0.117
3.86ThrAsp: 3.86 ± 0.342
3.886ThrGlu: 3.886 ± 0.353
2.009ThrPhe: 2.009 ± 0.201
4.098ThrGly: 4.098 ± 0.382
1.428ThrHis: 1.428 ± 0.189
4.838ThrIle: 4.838 ± 0.464
4.177ThrLys: 4.177 ± 0.329
4.838ThrLeu: 4.838 ± 0.361
1.243ThrMet: 1.243 ± 0.153
4.018ThrAsn: 4.018 ± 0.332
2.961ThrPro: 2.961 ± 0.31
2.776ThrGln: 2.776 ± 0.272
2.855ThrArg: 2.855 ± 0.343
4.812ThrSer: 4.812 ± 0.41
4.23ThrThr: 4.23 ± 0.514
4.283ThrVal: 4.283 ± 0.448
0.793ThrTrp: 0.793 ± 0.149
2.882ThrTyr: 2.882 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
4.256ValAla: 4.256 ± 0.346
0.344ValCys: 0.344 ± 0.089
3.992ValAsp: 3.992 ± 0.336
4.917ValGlu: 4.917 ± 0.442
2.459ValPhe: 2.459 ± 0.264
4.098ValGly: 4.098 ± 0.336
1.084ValHis: 1.084 ± 0.162
4.309ValIle: 4.309 ± 0.362
4.679ValLys: 4.679 ± 0.32
5.129ValLeu: 5.129 ± 0.433
1.348ValMet: 1.348 ± 0.246
3.992ValAsn: 3.992 ± 0.351
2.934ValPro: 2.934 ± 0.309
2.115ValGln: 2.115 ± 0.243
3.067ValArg: 3.067 ± 0.302
4.441ValSer: 4.441 ± 0.369
4.812ValThr: 4.812 ± 0.41
5.261ValVal: 5.261 ± 0.39
0.397ValTrp: 0.397 ± 0.112
3.543ValTyr: 3.543 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
0.529TrpAla: 0.529 ± 0.106
0.106TrpCys: 0.106 ± 0.051
0.687TrpAsp: 0.687 ± 0.121
0.634TrpGlu: 0.634 ± 0.133
0.449TrpPhe: 0.449 ± 0.108
0.555TrpGly: 0.555 ± 0.11
0.291TrpHis: 0.291 ± 0.107
0.925TrpIle: 0.925 ± 0.184
0.793TrpLys: 0.793 ± 0.143
0.899TrpLeu: 0.899 ± 0.141
0.317TrpMet: 0.317 ± 0.104
0.846TrpAsn: 0.846 ± 0.112
0.026TrpPro: 0.026 ± 0.028
0.317TrpGln: 0.317 ± 0.095
0.37TrpArg: 0.37 ± 0.087
1.057TrpSer: 1.057 ± 0.176
0.582TrpThr: 0.582 ± 0.116
0.661TrpVal: 0.661 ± 0.157
0.132TrpTrp: 0.132 ± 0.052
0.423TrpTyr: 0.423 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.432TyrAla: 2.432 ± 0.256
0.582TyrCys: 0.582 ± 0.152
2.512TyrAsp: 2.512 ± 0.294
2.934TyrGlu: 2.934 ± 0.31
1.718TyrPhe: 1.718 ± 0.23
2.67TyrGly: 2.67 ± 0.254
0.978TyrHis: 0.978 ± 0.178
3.384TyrIle: 3.384 ± 0.288
3.516TyrLys: 3.516 ± 0.309
4.838TyrLeu: 4.838 ± 0.385
1.163TyrMet: 1.163 ± 0.18
2.459TyrAsn: 2.459 ± 0.283
1.48TyrPro: 1.48 ± 0.212
1.903TyrGln: 1.903 ± 0.23
1.956TyrArg: 1.956 ± 0.248
3.067TyrSer: 3.067 ± 0.325
2.141TyrThr: 2.141 ± 0.247
2.274TyrVal: 2.274 ± 0.233
0.555TyrTrp: 0.555 ± 0.13
2.062TyrTyr: 2.062 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 144 proteins (37827 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski