Rheinheimera nanhaiensis E407-8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Rheinheimera; Rheinheimera nanhaiensis

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1DV32|I1DV32_9GAMM TetR family transcriptional regulator OS=Rheinheimera nanhaiensis E407-8 OX=562729 GN=RNAN_0880 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84NIYY5 pKa = 9.68KK6 pKa = 10.72ALLTGIALSCSAAPALAGSVSVGGVTWDD34 pKa = 3.82PDD36 pKa = 3.75FSQAFPPLDD45 pKa = 4.18DD46 pKa = 4.2FTAQTKK52 pKa = 8.57FAQWYY57 pKa = 8.7VGAATPTAGLGVGNIGVVPNPADD80 pKa = 4.57AIAVGAAGVGDD91 pKa = 3.81VLVGYY96 pKa = 9.26GIIDD100 pKa = 4.08VINGTGEE107 pKa = 3.85VDD109 pKa = 3.84FCTNYY114 pKa = 10.06PCRR117 pKa = 11.84LTYY120 pKa = 10.39TFGGFAITGLSASLNPQPRR139 pKa = 11.84PLFSGGWVNFYY150 pKa = 11.05LDD152 pKa = 3.44DD153 pKa = 3.82TAPFVGNPNLTNPSEE168 pKa = 4.11AATGNLWLSANAEE181 pKa = 4.2SFLNDD186 pKa = 3.49LGEE189 pKa = 4.27ATTLNFLEE197 pKa = 4.84GGIFTGSAEE206 pKa = 4.18AFLSVIGGAAFGNFDD221 pKa = 3.94TDD223 pKa = 3.15VFTAPGGAVADD234 pKa = 4.21FLYY237 pKa = 10.83SGNASFTNASGQLDD251 pKa = 4.01PTAMYY256 pKa = 8.03STNGNGQLRR265 pKa = 11.84GDD267 pKa = 4.96SIPEE271 pKa = 3.8PGILFLFGAGLVGLGVMRR289 pKa = 11.84KK290 pKa = 9.01KK291 pKa = 10.56AAA293 pKa = 3.49

Molecular weight: 29.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1DZX4|I1DZX4_9GAMM Replication protein A OS=Rheinheimera nanhaiensis E407-8 OX=562729 GN=RNAN_2608 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 9.37RR15 pKa = 11.84NHH17 pKa = 5.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.4NGRR29 pKa = 11.84QVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.91GRR40 pKa = 11.84KK41 pKa = 9.04RR42 pKa = 11.84LTAA45 pKa = 4.18

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3730

0

3730

1193890

37

12454

320.1

35.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.697 ± 0.045

0.981 ± 0.015

5.237 ± 0.033

5.004 ± 0.041

3.876 ± 0.025

6.76 ± 0.039

2.184 ± 0.019

5.204 ± 0.031

4.276 ± 0.041

11.875 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.021

3.714 ± 0.029

4.216 ± 0.027

6.26 ± 0.058

4.934 ± 0.027

6.33 ± 0.036

5.008 ± 0.028

6.761 ± 0.039

1.323 ± 0.019

3.106 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski