Streptococcus phage IPP51

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SDQ7|A0A1S5SDQ7_9CAUD Holin 1 OS=Streptococcus phage IPP51 OX=1916189 GN=IPP51_00046 PE=4 SV=1
MM1 pKa = 7.74VGVTYY6 pKa = 9.81QEE8 pKa = 3.08IHH10 pKa = 6.73LFVEE14 pKa = 5.07FLKK17 pKa = 10.63EE18 pKa = 3.59QYY20 pKa = 10.55GQGRR24 pKa = 11.84PDD26 pKa = 3.75YY27 pKa = 10.97IEE29 pKa = 4.84ALNDD33 pKa = 3.49LDD35 pKa = 4.31GLVEE39 pKa = 3.81VSYY42 pKa = 11.25RR43 pKa = 11.84EE44 pKa = 4.08AIEE47 pKa = 4.99RR48 pKa = 11.84FLEE51 pKa = 4.39DD52 pKa = 3.12EE53 pKa = 4.37VRR55 pKa = 4.08

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SDV5|A0A1S5SDV5_9CAUD Uncharacterized protein OS=Streptococcus phage IPP51 OX=1916189 GN=IPP51_00022 PE=4 SV=1
MM1 pKa = 7.21FFEE4 pKa = 4.59IARR7 pKa = 11.84FASILRR13 pKa = 11.84PKK15 pKa = 10.64YY16 pKa = 10.55LFLEE20 pKa = 4.29NVKK23 pKa = 10.75GLLNHH28 pKa = 6.4EE29 pKa = 4.5NGVTFEE35 pKa = 4.73TIISTLDD42 pKa = 3.13EE43 pKa = 3.82LRR45 pKa = 11.84YY46 pKa = 9.25NVEE49 pKa = 3.85WQVLNSKK56 pKa = 10.31NFGVPQNRR64 pKa = 11.84EE65 pKa = 3.39RR66 pKa = 11.84VFIIGHH72 pKa = 4.36SRR74 pKa = 11.84KK75 pKa = 9.71RR76 pKa = 11.84GTRR79 pKa = 11.84RR80 pKa = 11.84VFPMGQNN87 pKa = 3.14

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

10286

34

2134

214.3

24.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.455 ± 0.54

0.603 ± 0.13

6.047 ± 0.324

7.962 ± 0.353

4.2 ± 0.205

5.833 ± 0.29

1.633 ± 0.19

6.766 ± 0.325

8.487 ± 0.425

8.614 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.254

6.008 ± 0.296

2.547 ± 0.235

3.889 ± 0.254

4.676 ± 0.343

6.416 ± 0.513

6.368 ± 0.38

5.94 ± 0.225

1.478 ± 0.145

3.694 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski