Amino acid dipepetide frequency for Streptococcus phage IPP51

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.82AlaAla: 2.82 ± 0.808
0.389AlaCys: 0.389 ± 0.19
4.473AlaAsp: 4.473 ± 0.694
5.542AlaGlu: 5.542 ± 0.938
2.042AlaPhe: 2.042 ± 0.476
3.986AlaGly: 3.986 ± 0.951
0.583AlaHis: 0.583 ± 0.214
5.153AlaIle: 5.153 ± 1.435
5.25AlaLys: 5.25 ± 0.617
6.417AlaLeu: 6.417 ± 0.984
2.139AlaMet: 2.139 ± 0.453
3.209AlaAsn: 3.209 ± 0.665
1.167AlaPro: 1.167 ± 0.364
2.625AlaGln: 2.625 ± 0.792
2.528AlaArg: 2.528 ± 0.43
3.792AlaSer: 3.792 ± 0.511
4.57AlaThr: 4.57 ± 0.81
4.181AlaVal: 4.181 ± 0.792
0.972AlaTrp: 0.972 ± 0.38
2.625AlaTyr: 2.625 ± 0.461
0.0AlaXaa: 0.0 ± 0.0
Cys
0.194CysAla: 0.194 ± 0.12
0.0CysCys: 0.0 ± 0.0
0.292CysAsp: 0.292 ± 0.182
0.875CysGlu: 0.875 ± 0.319
0.194CysPhe: 0.194 ± 0.114
0.389CysGly: 0.389 ± 0.217
0.292CysHis: 0.292 ± 0.206
0.292CysIle: 0.292 ± 0.204
0.486CysLys: 0.486 ± 0.289
0.681CysLeu: 0.681 ± 0.298
0.0CysMet: 0.0 ± 0.0
0.097CysAsn: 0.097 ± 0.108
0.194CysPro: 0.194 ± 0.147
0.292CysGln: 0.292 ± 0.18
0.389CysArg: 0.389 ± 0.238
0.389CysSer: 0.389 ± 0.296
0.194CysThr: 0.194 ± 0.158
0.292CysVal: 0.292 ± 0.169
0.0CysTrp: 0.0 ± 0.0
0.486CysTyr: 0.486 ± 0.194
0.0CysXaa: 0.0 ± 0.0
Asp
3.5AspAla: 3.5 ± 0.638
0.292AspCys: 0.292 ± 0.161
3.889AspAsp: 3.889 ± 0.723
4.084AspGlu: 4.084 ± 1.017
3.014AspPhe: 3.014 ± 0.633
4.57AspGly: 4.57 ± 0.864
1.264AspHis: 1.264 ± 0.411
4.861AspIle: 4.861 ± 0.789
5.445AspLys: 5.445 ± 0.96
5.056AspLeu: 5.056 ± 0.553
1.264AspMet: 1.264 ± 0.352
3.306AspAsn: 3.306 ± 0.552
1.75AspPro: 1.75 ± 0.499
1.264AspGln: 1.264 ± 0.324
2.139AspArg: 2.139 ± 0.41
3.111AspSer: 3.111 ± 0.501
3.306AspThr: 3.306 ± 0.498
3.597AspVal: 3.597 ± 0.589
1.264AspTrp: 1.264 ± 0.397
3.5AspTyr: 3.5 ± 0.486
0.0AspXaa: 0.0 ± 0.0
Glu
5.25GluAla: 5.25 ± 1.426
0.583GluCys: 0.583 ± 0.286
3.5GluAsp: 3.5 ± 0.494
7.0GluGlu: 7.0 ± 1.118
3.306GluPhe: 3.306 ± 0.549
2.528GluGly: 2.528 ± 0.562
0.583GluHis: 0.583 ± 0.267
7.292GluIle: 7.292 ± 0.851
8.945GluLys: 8.945 ± 1.648
8.945GluLeu: 8.945 ± 0.781
1.75GluMet: 1.75 ± 0.486
4.861GluAsn: 4.861 ± 0.8
2.431GluPro: 2.431 ± 0.569
3.597GluGln: 3.597 ± 0.936
3.209GluArg: 3.209 ± 0.71
3.403GluSer: 3.403 ± 0.443
4.473GluThr: 4.473 ± 0.543
4.667GluVal: 4.667 ± 0.84
1.556GluTrp: 1.556 ± 0.449
1.75GluTyr: 1.75 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.528PheAla: 2.528 ± 0.591
0.292PheCys: 0.292 ± 0.183
4.278PheAsp: 4.278 ± 0.709
3.209PheGlu: 3.209 ± 0.498
1.458PhePhe: 1.458 ± 0.56
2.431PheGly: 2.431 ± 0.573
0.681PheHis: 0.681 ± 0.308
2.528PheIle: 2.528 ± 0.615
3.111PheLys: 3.111 ± 0.633
3.597PheLeu: 3.597 ± 1.012
1.847PheMet: 1.847 ± 0.686
3.306PheAsn: 3.306 ± 0.752
0.778PhePro: 0.778 ± 0.242
1.264PheGln: 1.264 ± 0.471
1.361PheArg: 1.361 ± 0.356
2.82PheSer: 2.82 ± 0.626
2.917PheThr: 2.917 ± 0.504
1.75PheVal: 1.75 ± 0.536
0.583PheTrp: 0.583 ± 0.231
1.264PheTyr: 1.264 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
3.597GlyAla: 3.597 ± 0.963
0.097GlyCys: 0.097 ± 0.106
2.236GlyAsp: 2.236 ± 0.416
3.5GlyGlu: 3.5 ± 0.589
3.403GlyPhe: 3.403 ± 0.632
3.5GlyGly: 3.5 ± 0.635
0.875GlyHis: 0.875 ± 0.264
4.57GlyIle: 4.57 ± 0.577
4.861GlyLys: 4.861 ± 0.574
5.056GlyLeu: 5.056 ± 0.882
2.139GlyMet: 2.139 ± 0.566
3.403GlyAsn: 3.403 ± 0.451
0.681GlyPro: 0.681 ± 0.292
3.014GlyGln: 3.014 ± 0.672
3.014GlyArg: 3.014 ± 0.582
3.597GlySer: 3.597 ± 0.693
3.403GlyThr: 3.403 ± 0.537
3.792GlyVal: 3.792 ± 0.435
1.361GlyTrp: 1.361 ± 0.592
2.236GlyTyr: 2.236 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
0.778HisAla: 0.778 ± 0.239
0.097HisCys: 0.097 ± 0.089
0.583HisAsp: 0.583 ± 0.312
1.264HisGlu: 1.264 ± 0.328
0.681HisPhe: 0.681 ± 0.245
0.778HisGly: 0.778 ± 0.293
0.0HisHis: 0.0 ± 0.0
1.75HisIle: 1.75 ± 0.319
0.972HisLys: 0.972 ± 0.278
1.653HisLeu: 1.653 ± 0.493
0.292HisMet: 0.292 ± 0.164
1.07HisAsn: 1.07 ± 0.305
0.583HisPro: 0.583 ± 0.223
0.681HisGln: 0.681 ± 0.278
0.583HisArg: 0.583 ± 0.29
1.458HisSer: 1.458 ± 0.515
1.07HisThr: 1.07 ± 0.457
0.778HisVal: 0.778 ± 0.295
0.0HisTrp: 0.0 ± 0.0
1.264HisTyr: 1.264 ± 0.526
0.0HisXaa: 0.0 ± 0.0
Ile
5.834IleAla: 5.834 ± 0.832
0.681IleCys: 0.681 ± 0.379
5.056IleAsp: 5.056 ± 0.688
6.32IleGlu: 6.32 ± 1.061
2.625IlePhe: 2.625 ± 0.578
4.084IleGly: 4.084 ± 0.729
1.07IleHis: 1.07 ± 0.348
3.5IleIle: 3.5 ± 0.904
5.056IleLys: 5.056 ± 0.736
4.959IleLeu: 4.959 ± 0.713
1.361IleMet: 1.361 ± 0.331
4.375IleAsn: 4.375 ± 0.621
1.653IlePro: 1.653 ± 0.3
2.528IleGln: 2.528 ± 0.425
3.111IleArg: 3.111 ± 0.656
5.639IleSer: 5.639 ± 0.869
3.986IleThr: 3.986 ± 0.41
3.403IleVal: 3.403 ± 0.627
0.972IleTrp: 0.972 ± 0.279
1.458IleTyr: 1.458 ± 0.505
0.0IleXaa: 0.0 ± 0.0
Lys
5.931LysAla: 5.931 ± 1.003
0.292LysCys: 0.292 ± 0.214
5.834LysAsp: 5.834 ± 0.782
7.487LysGlu: 7.487 ± 1.167
2.431LysPhe: 2.431 ± 0.507
4.57LysGly: 4.57 ± 0.8
1.945LysHis: 1.945 ± 0.373
4.861LysIle: 4.861 ± 0.819
5.931LysLys: 5.931 ± 1.261
7.098LysLeu: 7.098 ± 0.976
2.625LysMet: 2.625 ± 0.434
4.667LysAsn: 4.667 ± 0.569
1.945LysPro: 1.945 ± 0.518
3.889LysGln: 3.889 ± 0.49
3.5LysArg: 3.5 ± 0.729
5.834LysSer: 5.834 ± 0.579
5.931LysThr: 5.931 ± 0.617
5.153LysVal: 5.153 ± 0.918
0.875LysTrp: 0.875 ± 0.295
4.084LysTyr: 4.084 ± 0.697
0.0LysXaa: 0.0 ± 0.0
Leu
6.223LeuAla: 6.223 ± 0.784
0.292LeuCys: 0.292 ± 0.25
6.32LeuAsp: 6.32 ± 0.785
7.098LeuGlu: 7.098 ± 0.936
3.209LeuPhe: 3.209 ± 0.554
5.737LeuGly: 5.737 ± 0.777
1.556LeuHis: 1.556 ± 0.518
5.445LeuIle: 5.445 ± 0.578
7.973LeuLys: 7.973 ± 0.784
5.639LeuLeu: 5.639 ± 1.033
2.236LeuMet: 2.236 ± 0.609
5.834LeuAsn: 5.834 ± 0.807
3.111LeuPro: 3.111 ± 0.739
2.139LeuGln: 2.139 ± 0.581
4.375LeuArg: 4.375 ± 0.688
7.195LeuSer: 7.195 ± 0.964
5.056LeuThr: 5.056 ± 0.866
3.695LeuVal: 3.695 ± 0.572
0.583LeuTrp: 0.583 ± 0.378
2.431LeuTyr: 2.431 ± 0.478
0.0LeuXaa: 0.0 ± 0.0
Met
1.361MetAla: 1.361 ± 0.361
0.486MetCys: 0.486 ± 0.28
0.875MetAsp: 0.875 ± 0.321
2.528MetGlu: 2.528 ± 0.659
0.389MetPhe: 0.389 ± 0.245
1.458MetGly: 1.458 ± 0.325
0.194MetHis: 0.194 ± 0.146
1.458MetIle: 1.458 ± 0.503
2.625MetLys: 2.625 ± 0.558
2.139MetLeu: 2.139 ± 0.471
0.389MetMet: 0.389 ± 0.204
1.945MetAsn: 1.945 ± 0.558
0.875MetPro: 0.875 ± 0.365
0.681MetGln: 0.681 ± 0.281
0.972MetArg: 0.972 ± 0.313
1.264MetSer: 1.264 ± 0.572
1.556MetThr: 1.556 ± 0.416
1.945MetVal: 1.945 ± 0.386
0.097MetTrp: 0.097 ± 0.105
0.583MetTyr: 0.583 ± 0.234
0.0MetXaa: 0.0 ± 0.0
Asn
3.014AsnAla: 3.014 ± 0.785
0.097AsnCys: 0.097 ± 0.074
3.209AsnAsp: 3.209 ± 0.74
4.473AsnGlu: 4.473 ± 0.403
2.917AsnPhe: 2.917 ± 0.491
3.597AsnGly: 3.597 ± 0.763
1.361AsnHis: 1.361 ± 0.374
3.889AsnIle: 3.889 ± 0.655
4.667AsnLys: 4.667 ± 0.735
5.348AsnLeu: 5.348 ± 0.781
0.972AsnMet: 0.972 ± 0.447
3.597AsnAsn: 3.597 ± 0.659
2.236AsnPro: 2.236 ± 0.459
3.014AsnGln: 3.014 ± 0.659
3.792AsnArg: 3.792 ± 0.58
3.792AsnSer: 3.792 ± 1.001
3.209AsnThr: 3.209 ± 0.494
3.792AsnVal: 3.792 ± 0.659
1.07AsnTrp: 1.07 ± 0.288
2.042AsnTyr: 2.042 ± 0.473
0.0AsnXaa: 0.0 ± 0.0
Pro
1.945ProAla: 1.945 ± 0.359
0.389ProCys: 0.389 ± 0.232
1.945ProAsp: 1.945 ± 0.432
1.653ProGlu: 1.653 ± 0.47
0.875ProPhe: 0.875 ± 0.364
0.972ProGly: 0.972 ± 0.325
0.486ProHis: 0.486 ± 0.209
1.556ProIle: 1.556 ± 0.549
3.014ProLys: 3.014 ± 0.441
2.431ProLeu: 2.431 ± 0.541
0.486ProMet: 0.486 ± 0.269
0.972ProAsn: 0.972 ± 0.321
0.389ProPro: 0.389 ± 0.252
1.556ProGln: 1.556 ± 0.473
1.167ProArg: 1.167 ± 0.428
1.458ProSer: 1.458 ± 0.411
1.264ProThr: 1.264 ± 0.43
1.847ProVal: 1.847 ± 0.333
0.389ProTrp: 0.389 ± 0.171
0.681ProTyr: 0.681 ± 0.341
0.0ProXaa: 0.0 ± 0.0
Gln
3.792GlnAla: 3.792 ± 0.619
0.097GlnCys: 0.097 ± 0.108
1.264GlnAsp: 1.264 ± 0.305
4.181GlnGlu: 4.181 ± 0.821
1.167GlnPhe: 1.167 ± 0.247
1.75GlnGly: 1.75 ± 0.361
0.292GlnHis: 0.292 ± 0.154
2.236GlnIle: 2.236 ± 0.42
3.111GlnLys: 3.111 ± 0.499
3.695GlnLeu: 3.695 ± 0.58
0.681GlnMet: 0.681 ± 0.237
2.625GlnAsn: 2.625 ± 0.484
1.07GlnPro: 1.07 ± 0.265
1.167GlnGln: 1.167 ± 0.297
2.431GlnArg: 2.431 ± 0.59
2.917GlnSer: 2.917 ± 0.585
2.431GlnThr: 2.431 ± 0.473
2.82GlnVal: 2.82 ± 0.551
0.292GlnTrp: 0.292 ± 0.163
0.875GlnTyr: 0.875 ± 0.331
0.0GlnXaa: 0.0 ± 0.0
Arg
3.014ArgAla: 3.014 ± 0.458
0.486ArgCys: 0.486 ± 0.209
2.139ArgAsp: 2.139 ± 0.417
3.5ArgGlu: 3.5 ± 0.58
2.042ArgPhe: 2.042 ± 0.429
1.945ArgGly: 1.945 ± 0.447
0.972ArgHis: 0.972 ± 0.356
3.209ArgIle: 3.209 ± 0.711
4.181ArgLys: 4.181 ± 0.634
4.278ArgLeu: 4.278 ± 0.632
1.167ArgMet: 1.167 ± 0.285
2.722ArgAsn: 2.722 ± 0.577
1.556ArgPro: 1.556 ± 0.442
2.625ArgGln: 2.625 ± 0.611
2.333ArgArg: 2.333 ± 0.61
2.139ArgSer: 2.139 ± 0.357
3.209ArgThr: 3.209 ± 0.935
2.431ArgVal: 2.431 ± 0.492
0.681ArgTrp: 0.681 ± 0.216
2.139ArgTyr: 2.139 ± 0.557
0.0ArgXaa: 0.0 ± 0.0
Ser
4.764SerAla: 4.764 ± 1.032
0.292SerCys: 0.292 ± 0.173
3.889SerAsp: 3.889 ± 0.733
4.278SerGlu: 4.278 ± 0.737
3.111SerPhe: 3.111 ± 0.514
4.181SerGly: 4.181 ± 0.864
0.875SerHis: 0.875 ± 0.361
3.792SerIle: 3.792 ± 0.619
4.861SerLys: 4.861 ± 0.649
4.667SerLeu: 4.667 ± 0.814
0.972SerMet: 0.972 ± 0.378
4.278SerAsn: 4.278 ± 0.812
1.556SerPro: 1.556 ± 0.38
1.945SerGln: 1.945 ± 0.55
3.5SerArg: 3.5 ± 0.81
5.153SerSer: 5.153 ± 1.367
4.764SerThr: 4.764 ± 0.853
3.986SerVal: 3.986 ± 0.923
1.07SerTrp: 1.07 ± 0.309
2.236SerTyr: 2.236 ± 0.399
0.0SerXaa: 0.0 ± 0.0
Thr
4.375ThrAla: 4.375 ± 1.088
0.389ThrCys: 0.389 ± 0.208
3.792ThrAsp: 3.792 ± 0.655
3.5ThrGlu: 3.5 ± 0.654
3.5ThrPhe: 3.5 ± 0.761
5.056ThrGly: 5.056 ± 0.942
0.972ThrHis: 0.972 ± 0.286
5.056ThrIle: 5.056 ± 0.544
4.764ThrLys: 4.764 ± 0.518
5.056ThrLeu: 5.056 ± 0.816
0.583ThrMet: 0.583 ± 0.255
3.014ThrAsn: 3.014 ± 0.576
0.972ThrPro: 0.972 ± 0.386
2.625ThrGln: 2.625 ± 0.496
2.722ThrArg: 2.722 ± 0.531
4.181ThrSer: 4.181 ± 0.672
5.056ThrThr: 5.056 ± 1.008
4.667ThrVal: 4.667 ± 0.54
1.167ThrTrp: 1.167 ± 0.395
2.236ThrTyr: 2.236 ± 0.495
0.0ThrXaa: 0.0 ± 0.0
Val
2.82ValAla: 2.82 ± 0.492
0.389ValCys: 0.389 ± 0.19
4.084ValAsp: 4.084 ± 0.483
6.125ValGlu: 6.125 ± 0.657
3.111ValPhe: 3.111 ± 0.81
3.986ValGly: 3.986 ± 0.771
1.07ValHis: 1.07 ± 0.337
2.431ValIle: 2.431 ± 0.453
5.639ValLys: 5.639 ± 0.954
3.5ValLeu: 3.5 ± 0.66
1.264ValMet: 1.264 ± 0.325
3.695ValAsn: 3.695 ± 0.458
1.458ValPro: 1.458 ± 0.32
1.75ValGln: 1.75 ± 0.375
2.722ValArg: 2.722 ± 0.582
3.111ValSer: 3.111 ± 0.578
5.056ValThr: 5.056 ± 0.93
4.084ValVal: 4.084 ± 0.56
1.07ValTrp: 1.07 ± 0.284
2.042ValTyr: 2.042 ± 0.603
0.0ValXaa: 0.0 ± 0.0
Trp
0.583TrpAla: 0.583 ± 0.217
0.097TrpCys: 0.097 ± 0.118
0.486TrpAsp: 0.486 ± 0.26
0.778TrpGlu: 0.778 ± 0.223
0.681TrpPhe: 0.681 ± 0.313
1.264TrpGly: 1.264 ± 0.308
0.292TrpHis: 0.292 ± 0.183
1.458TrpIle: 1.458 ± 0.375
0.778TrpLys: 0.778 ± 0.282
1.361TrpLeu: 1.361 ± 0.453
0.583TrpMet: 0.583 ± 0.207
1.361TrpAsn: 1.361 ± 0.389
0.097TrpPro: 0.097 ± 0.093
0.389TrpGln: 0.389 ± 0.153
0.486TrpArg: 0.486 ± 0.205
1.167TrpSer: 1.167 ± 0.535
0.875TrpThr: 0.875 ± 0.244
0.972TrpVal: 0.972 ± 0.264
0.097TrpTrp: 0.097 ± 0.074
0.972TrpTyr: 0.972 ± 0.666
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.236TyrAla: 2.236 ± 0.407
0.292TyrCys: 0.292 ± 0.15
2.333TyrAsp: 2.333 ± 0.506
2.236TyrGlu: 2.236 ± 0.504
1.847TyrPhe: 1.847 ± 0.56
1.556TyrGly: 1.556 ± 0.457
0.972TyrHis: 0.972 ± 0.268
2.333TyrIle: 2.333 ± 0.472
3.209TyrLys: 3.209 ± 0.744
4.57TyrLeu: 4.57 ± 0.716
0.972TyrMet: 0.972 ± 0.39
1.75TyrAsn: 1.75 ± 0.494
0.972TyrPro: 0.972 ± 0.305
1.847TyrGln: 1.847 ± 0.479
2.431TyrArg: 2.431 ± 0.571
1.75TyrSer: 1.75 ± 0.305
1.361TyrThr: 1.361 ± 0.335
1.556TyrVal: 1.556 ± 0.372
0.681TyrTrp: 0.681 ± 0.254
2.042TyrTyr: 2.042 ± 0.945
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (10286 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski