Lactococcus allomyrinae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2440 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A387BH84|A0A387BH84_9LACT DUF3397 domain-containing protein OS=Lactococcus allomyrinae OX=2419773 GN=D7I46_12050 PE=4 SV=1
MM1 pKa = 7.42NKK3 pKa = 9.98LVLKK7 pKa = 10.77SSALLATVLIAGPVLISTAGAATTTGGDD35 pKa = 3.7YY36 pKa = 10.64TSSANLTLQAGTGPVDD52 pKa = 3.92PLDD55 pKa = 4.16PTDD58 pKa = 3.65PTNPVTPVDD67 pKa = 4.31PGTPGAPGNLTVDD80 pKa = 3.9YY81 pKa = 11.04GSTLYY86 pKa = 10.82FGTQSISTQAQTYY99 pKa = 7.67YY100 pKa = 10.65AHH102 pKa = 7.87PDD104 pKa = 3.37MVVDD108 pKa = 5.2KK109 pKa = 11.13DD110 pKa = 3.84DD111 pKa = 3.51ATKK114 pKa = 10.32IVPNYY119 pKa = 10.38AQVTDD124 pKa = 3.65QSGDD128 pKa = 3.4LAGWTLTLTQASDD141 pKa = 3.33LHH143 pKa = 7.09LSSGSSSDD151 pKa = 3.04AGYY154 pKa = 11.22ALTGAQINFTGGTIVGGNGITSGTPSDD181 pKa = 3.96VVTSGSLTPGVATKK195 pKa = 10.53LVSAAEE201 pKa = 4.04NEE203 pKa = 4.71GAGTWLYY210 pKa = 11.6NFGDD214 pKa = 3.41VDD216 pKa = 4.12AYY218 pKa = 10.91DD219 pKa = 3.86AASVDD224 pKa = 3.79TANTSDD230 pKa = 3.44QSKK233 pKa = 9.78VATKK237 pKa = 10.46SAISLTVPSGLVEE250 pKa = 3.9KK251 pKa = 10.45AAAYY255 pKa = 6.94TTDD258 pKa = 5.0LYY260 pKa = 10.7WSLQAVPGNDD270 pKa = 2.74WSGNTSAVSQQ280 pKa = 3.74

Molecular weight: 28.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A387BGX7|A0A387BGX7_9LACT Uncharacterized protein OS=Lactococcus allomyrinae OX=2419773 GN=D7I46_02870 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.06QPHH8 pKa = 5.63KK9 pKa = 9.98KK10 pKa = 9.63SRR12 pKa = 11.84KK13 pKa = 4.36TTHH16 pKa = 6.07GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.11NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.65GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2440

0

2440

725829

19

2644

297.5

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.524 ± 0.065

0.477 ± 0.013

5.322 ± 0.045

6.805 ± 0.062

4.855 ± 0.044

6.611 ± 0.06

1.85 ± 0.024

7.541 ± 0.048

6.917 ± 0.052

9.757 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.025

5.111 ± 0.046

3.297 ± 0.032

3.783 ± 0.031

3.769 ± 0.037

6.45 ± 0.044

6.107 ± 0.059

6.76 ± 0.04

1.02 ± 0.02

3.537 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski