Lactobacillus phage LBR48
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6PST9|D6PST9_9CAUD Uncharacterized protein OS=Lactobacillus phage LBR48 OX=755164 PE=4 SV=1
MM1 pKa = 7.76 IAPDD5 pKa = 3.76 HH6 pKa = 6.32 ATFDD10 pKa = 4.16 LNNYY14 pKa = 7.2 MNRR17 pKa = 11.84 LDD19 pKa = 4.55 KK20 pKa = 11.23 DD21 pKa = 3.65 SAEE24 pKa = 4.54 DD25 pKa = 3.41 KK26 pKa = 9.64 QTYY29 pKa = 10.31 DD30 pKa = 2.8 IDD32 pKa = 4.24 GFPLIYY38 pKa = 10.38 GEE40 pKa = 5.18 KK41 pKa = 10.33 YY42 pKa = 6.57 WTLDD46 pKa = 3.1 DD47 pKa = 3.88 GRR49 pKa = 11.84 YY50 pKa = 10.59 VMDD53 pKa = 5.6 DD54 pKa = 3.4 AASMHH59 pKa = 6.91 DD60 pKa = 3.92 FLDD63 pKa = 4.65 SEE65 pKa = 4.68 HH66 pKa = 7.21 EE67 pKa = 4.38 DD68 pKa = 3.44 YY69 pKa = 11.43 GDD71 pKa = 5.03 PIDD74 pKa = 4.16 YY75 pKa = 10.38 DD76 pKa = 3.8 YY77 pKa = 12.09 GNLAKK82 pKa = 10.38 IMEE85 pKa = 4.45 EE86 pKa = 4.03 FKK88 pKa = 10.3 GTEE91 pKa = 3.91 VISWIKK97 pKa = 9.63 YY98 pKa = 7.96
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.961
IPC_protein 3.948
Toseland 3.719
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.77
Grimsley 3.63
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.329
Thurlkill 3.783
EMBOSS 3.91
Sillero 4.075
Patrickios 1.939
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.953
Protein with the highest isoelectric point:
>tr|D6PSU6|D6PSU6_9CAUD Uncharacterized protein OS=Lactobacillus phage LBR48 OX=755164 PE=4 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 VRR4 pKa = 11.84 LTSSKK9 pKa = 10.59 GYY11 pKa = 9.85 HH12 pKa = 5.74 EE13 pKa = 5.69 CGLSYY18 pKa = 11.27 ARR20 pKa = 11.84 VHH22 pKa = 5.58 YY23 pKa = 10.38 LGRR26 pKa = 11.84 TPTWKK31 pKa = 9.99 IKK33 pKa = 10.22 AAIWQQKK40 pKa = 9.7 KK41 pKa = 9.55 PVINGWRR48 pKa = 11.84 VEE50 pKa = 4.29 VIKK53 pKa = 11.01
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.984
IPC_protein 10.54
Toseland 10.73
ProMoST 10.423
Dawson 10.847
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.286
Grimsley 10.891
Solomon 10.906
Lehninger 10.891
Nozaki 10.716
DTASelect 10.511
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.76
Patrickios 11.052
IPC_peptide 10.921
IPC2_peptide 9.385
IPC2.peptide.svr19 8.382
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
13349
29
512
155.2
17.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.15 ± 0.388
0.569 ± 0.067
7.064 ± 0.376
5.573 ± 0.27
3.438 ± 0.176
6.75 ± 0.243
2.135 ± 0.157
5.918 ± 0.283
7.993 ± 0.416
7.566 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.862 ± 0.141
5.049 ± 0.202
3.394 ± 0.184
4.113 ± 0.198
4.727 ± 0.314
7.199 ± 0.394
6.105 ± 0.288
6.315 ± 0.269
1.491 ± 0.126
3.581 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here