Amino acid dipepetide frequency for Lactobacillus phage LBR48

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.84AlaAla: 8.84 ± 1.286
0.375AlaCys: 0.375 ± 0.164
5.844AlaAsp: 5.844 ± 0.764
4.57AlaGlu: 4.57 ± 0.561
2.023AlaPhe: 2.023 ± 0.384
5.319AlaGly: 5.319 ± 0.743
1.498AlaHis: 1.498 ± 0.307
6.293AlaIle: 6.293 ± 0.744
7.192AlaLys: 7.192 ± 0.841
5.844AlaLeu: 5.844 ± 0.705
1.723AlaMet: 1.723 ± 0.441
3.896AlaAsn: 3.896 ± 0.493
2.023AlaPro: 2.023 ± 0.388
4.495AlaGln: 4.495 ± 0.563
2.772AlaArg: 2.772 ± 0.477
5.544AlaSer: 5.544 ± 0.828
5.544AlaThr: 5.544 ± 0.667
4.645AlaVal: 4.645 ± 0.647
0.899AlaTrp: 0.899 ± 0.253
2.173AlaTyr: 2.173 ± 0.395
0.0AlaXaa: 0.0 ± 0.0
Cys
0.225CysAla: 0.225 ± 0.128
0.075CysCys: 0.075 ± 0.078
0.3CysAsp: 0.3 ± 0.159
0.075CysGlu: 0.075 ± 0.074
0.15CysPhe: 0.15 ± 0.11
0.749CysGly: 0.749 ± 0.256
0.075CysHis: 0.075 ± 0.082
0.225CysIle: 0.225 ± 0.125
0.225CysLys: 0.225 ± 0.118
0.749CysLeu: 0.749 ± 0.23
0.375CysMet: 0.375 ± 0.186
0.075CysAsn: 0.075 ± 0.069
0.15CysPro: 0.15 ± 0.1
0.3CysGln: 0.3 ± 0.166
0.375CysArg: 0.375 ± 0.148
0.225CysSer: 0.225 ± 0.12
0.45CysThr: 0.45 ± 0.171
0.3CysVal: 0.3 ± 0.124
0.075CysTrp: 0.075 ± 0.072
0.524CysTyr: 0.524 ± 0.198
0.0CysXaa: 0.0 ± 0.0
Asp
4.645AspAla: 4.645 ± 0.838
0.45AspCys: 0.45 ± 0.198
6.143AspAsp: 6.143 ± 0.702
4.72AspGlu: 4.72 ± 0.548
2.772AspPhe: 2.772 ± 0.37
4.87AspGly: 4.87 ± 0.688
1.498AspHis: 1.498 ± 0.316
3.296AspIle: 3.296 ± 0.536
5.244AspLys: 5.244 ± 0.584
5.394AspLeu: 5.394 ± 0.747
1.648AspMet: 1.648 ± 0.328
2.847AspAsn: 2.847 ± 0.375
2.697AspPro: 2.697 ± 0.449
2.173AspGln: 2.173 ± 0.415
3.072AspArg: 3.072 ± 0.518
4.645AspSer: 4.645 ± 0.652
4.645AspThr: 4.645 ± 0.579
5.394AspVal: 5.394 ± 0.612
1.573AspTrp: 1.573 ± 0.314
2.922AspTyr: 2.922 ± 0.528
0.0AspXaa: 0.0 ± 0.0
Glu
4.12GluAla: 4.12 ± 0.484
0.375GluCys: 0.375 ± 0.168
4.27GluAsp: 4.27 ± 0.583
2.847GluGlu: 2.847 ± 0.543
2.098GluPhe: 2.098 ± 0.393
2.397GluGly: 2.397 ± 0.304
1.349GluHis: 1.349 ± 0.294
3.072GluIle: 3.072 ± 0.486
3.821GluLys: 3.821 ± 0.602
5.019GluLeu: 5.019 ± 0.604
1.798GluMet: 1.798 ± 0.443
2.322GluAsn: 2.322 ± 0.399
1.423GluPro: 1.423 ± 0.276
2.997GluGln: 2.997 ± 0.435
3.896GluArg: 3.896 ± 0.745
3.896GluSer: 3.896 ± 0.451
3.072GluThr: 3.072 ± 0.494
4.046GluVal: 4.046 ± 0.538
0.749GluTrp: 0.749 ± 0.204
2.173GluTyr: 2.173 ± 0.409
0.0GluXaa: 0.0 ± 0.0
Phe
2.322PheAla: 2.322 ± 0.401
0.599PheCys: 0.599 ± 0.25
3.147PheAsp: 3.147 ± 0.497
1.798PheGlu: 1.798 ± 0.42
1.573PhePhe: 1.573 ± 0.46
3.147PheGly: 3.147 ± 0.551
0.749PheHis: 0.749 ± 0.205
1.423PheIle: 1.423 ± 0.274
2.997PheLys: 2.997 ± 0.427
1.723PheLeu: 1.723 ± 0.385
1.274PheMet: 1.274 ± 0.254
1.948PheAsn: 1.948 ± 0.38
1.199PhePro: 1.199 ± 0.314
0.749PheGln: 0.749 ± 0.272
1.798PheArg: 1.798 ± 0.386
2.922PheSer: 2.922 ± 0.505
2.173PheThr: 2.173 ± 0.375
1.049PheVal: 1.049 ± 0.314
0.375PheTrp: 0.375 ± 0.201
1.423PheTyr: 1.423 ± 0.433
0.0PheXaa: 0.0 ± 0.0
Gly
5.169GlyAla: 5.169 ± 0.734
0.45GlyCys: 0.45 ± 0.173
4.57GlyAsp: 4.57 ± 0.609
4.27GlyGlu: 4.27 ± 0.582
2.173GlyPhe: 2.173 ± 0.37
4.795GlyGly: 4.795 ± 0.656
1.349GlyHis: 1.349 ± 0.322
4.42GlyIle: 4.42 ± 0.578
6.218GlyLys: 6.218 ± 0.832
5.394GlyLeu: 5.394 ± 0.793
2.397GlyMet: 2.397 ± 0.346
2.772GlyAsn: 2.772 ± 0.519
1.274GlyPro: 1.274 ± 0.303
1.948GlyGln: 1.948 ± 0.364
2.322GlyArg: 2.322 ± 0.417
5.544GlySer: 5.544 ± 0.695
4.27GlyThr: 4.27 ± 0.59
3.671GlyVal: 3.671 ± 0.494
1.723GlyTrp: 1.723 ± 0.372
2.697GlyTyr: 2.697 ± 0.4
0.0GlyXaa: 0.0 ± 0.0
His
1.573HisAla: 1.573 ± 0.389
0.225HisCys: 0.225 ± 0.136
1.423HisAsp: 1.423 ± 0.262
1.798HisGlu: 1.798 ± 0.422
1.349HisPhe: 1.349 ± 0.263
2.397HisGly: 2.397 ± 0.439
0.375HisHis: 0.375 ± 0.19
1.274HisIle: 1.274 ± 0.293
1.423HisLys: 1.423 ± 0.294
1.349HisLeu: 1.349 ± 0.266
0.3HisMet: 0.3 ± 0.176
1.199HisAsn: 1.199 ± 0.406
0.599HisPro: 0.599 ± 0.194
0.749HisGln: 0.749 ± 0.262
1.199HisArg: 1.199 ± 0.357
1.049HisSer: 1.049 ± 0.245
0.749HisThr: 0.749 ± 0.194
1.274HisVal: 1.274 ± 0.273
0.45HisTrp: 0.45 ± 0.169
0.599HisTyr: 0.599 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
5.169IleAla: 5.169 ± 0.484
0.075IleCys: 0.075 ± 0.077
3.971IleAsp: 3.971 ± 0.669
3.596IleGlu: 3.596 ± 0.6
1.948IlePhe: 1.948 ± 0.392
3.147IleGly: 3.147 ± 0.509
1.498IleHis: 1.498 ± 0.297
2.472IleIle: 2.472 ± 0.585
4.345IleLys: 4.345 ± 0.501
4.42IleLeu: 4.42 ± 0.495
1.573IleMet: 1.573 ± 0.343
4.27IleAsn: 4.27 ± 0.586
2.847IlePro: 2.847 ± 0.497
2.248IleGln: 2.248 ± 0.342
2.997IleArg: 2.997 ± 0.549
3.896IleSer: 3.896 ± 0.559
4.72IleThr: 4.72 ± 0.476
3.147IleVal: 3.147 ± 0.44
0.45IleTrp: 0.45 ± 0.177
2.397IleTyr: 2.397 ± 0.401
0.0IleXaa: 0.0 ± 0.0
Lys
6.518LysAla: 6.518 ± 0.904
0.225LysCys: 0.225 ± 0.104
5.019LysAsp: 5.019 ± 0.672
4.046LysGlu: 4.046 ± 0.584
2.622LysPhe: 2.622 ± 0.452
5.319LysGly: 5.319 ± 0.73
1.573LysHis: 1.573 ± 0.334
4.12LysIle: 4.12 ± 0.562
6.967LysLys: 6.967 ± 0.926
5.993LysLeu: 5.993 ± 0.7
3.147LysMet: 3.147 ± 0.454
3.521LysAsn: 3.521 ± 0.66
3.371LysPro: 3.371 ± 0.561
4.195LysGln: 4.195 ± 0.595
4.345LysArg: 4.345 ± 0.618
6.443LysSer: 6.443 ± 0.757
4.046LysThr: 4.046 ± 0.598
4.195LysVal: 4.195 ± 0.442
1.498LysTrp: 1.498 ± 0.334
2.772LysTyr: 2.772 ± 0.445
0.0LysXaa: 0.0 ± 0.0
Leu
5.918LeuAla: 5.918 ± 0.854
0.599LeuCys: 0.599 ± 0.182
4.72LeuAsp: 4.72 ± 0.674
4.12LeuGlu: 4.12 ± 0.499
2.397LeuPhe: 2.397 ± 0.36
4.495LeuGly: 4.495 ± 0.593
1.498LeuHis: 1.498 ± 0.375
4.42LeuIle: 4.42 ± 0.653
6.967LeuLys: 6.967 ± 0.693
5.469LeuLeu: 5.469 ± 0.882
1.573LeuMet: 1.573 ± 0.341
4.495LeuAsn: 4.495 ± 0.612
2.622LeuPro: 2.622 ± 0.403
3.746LeuGln: 3.746 ± 0.587
3.296LeuArg: 3.296 ± 0.615
5.319LeuSer: 5.319 ± 0.598
4.87LeuThr: 4.87 ± 0.635
5.244LeuVal: 5.244 ± 0.813
1.349LeuTrp: 1.349 ± 0.357
2.472LeuTyr: 2.472 ± 0.485
0.075LeuXaa: 0.075 ± 0.07
Met
3.446MetAla: 3.446 ± 0.384
0.075MetCys: 0.075 ± 0.073
2.098MetAsp: 2.098 ± 0.44
1.723MetGlu: 1.723 ± 0.368
0.599MetPhe: 0.599 ± 0.212
2.248MetGly: 2.248 ± 0.41
0.524MetHis: 0.524 ± 0.184
1.423MetIle: 1.423 ± 0.338
2.622MetLys: 2.622 ± 0.478
1.873MetLeu: 1.873 ± 0.338
0.3MetMet: 0.3 ± 0.158
1.274MetAsn: 1.274 ± 0.276
1.423MetPro: 1.423 ± 0.309
0.824MetGln: 0.824 ± 0.283
1.349MetArg: 1.349 ± 0.307
2.248MetSer: 2.248 ± 0.394
1.798MetThr: 1.798 ± 0.321
2.023MetVal: 2.023 ± 0.388
0.075MetTrp: 0.075 ± 0.072
0.674MetTyr: 0.674 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
3.896AsnAla: 3.896 ± 0.569
0.15AsnCys: 0.15 ± 0.099
3.596AsnAsp: 3.596 ± 0.578
2.472AsnGlu: 2.472 ± 0.45
1.573AsnPhe: 1.573 ± 0.297
4.195AsnGly: 4.195 ± 0.428
1.648AsnHis: 1.648 ± 0.328
2.472AsnIle: 2.472 ± 0.429
3.596AsnLys: 3.596 ± 0.551
3.971AsnLeu: 3.971 ± 0.56
0.599AsnMet: 0.599 ± 0.211
2.622AsnAsn: 2.622 ± 0.465
2.547AsnPro: 2.547 ± 0.418
2.472AsnGln: 2.472 ± 0.449
2.098AsnArg: 2.098 ± 0.373
4.046AsnSer: 4.046 ± 0.674
2.023AsnThr: 2.023 ± 0.348
3.072AsnVal: 3.072 ± 0.518
1.049AsnTrp: 1.049 ± 0.346
2.397AsnTyr: 2.397 ± 0.356
0.0AsnXaa: 0.0 ± 0.0
Pro
3.147ProAla: 3.147 ± 0.515
0.15ProCys: 0.15 ± 0.102
3.221ProAsp: 3.221 ± 0.413
2.248ProGlu: 2.248 ± 0.369
0.899ProPhe: 0.899 ± 0.298
2.023ProGly: 2.023 ± 0.326
1.124ProHis: 1.124 ± 0.332
2.248ProIle: 2.248 ± 0.379
2.622ProLys: 2.622 ± 0.556
1.798ProLeu: 1.798 ± 0.412
1.199ProMet: 1.199 ± 0.33
1.498ProAsn: 1.498 ± 0.349
0.899ProPro: 0.899 ± 0.351
1.349ProGln: 1.349 ± 0.383
1.124ProArg: 1.124 ± 0.368
2.397ProSer: 2.397 ± 0.521
2.397ProThr: 2.397 ± 0.406
2.098ProVal: 2.098 ± 0.421
0.375ProTrp: 0.375 ± 0.179
1.124ProTyr: 1.124 ± 0.32
0.0ProXaa: 0.0 ± 0.0
Gln
3.746GlnAla: 3.746 ± 0.564
0.075GlnCys: 0.075 ± 0.076
1.873GlnAsp: 1.873 ± 0.337
2.547GlnGlu: 2.547 ± 0.558
1.199GlnPhe: 1.199 ± 0.311
1.723GlnGly: 1.723 ± 0.347
0.899GlnHis: 0.899 ± 0.261
2.622GlnIle: 2.622 ± 0.462
2.847GlnLys: 2.847 ± 0.577
4.345GlnLeu: 4.345 ± 0.684
1.723GlnMet: 1.723 ± 0.456
2.322GlnAsn: 2.322 ± 0.369
1.349GlnPro: 1.349 ± 0.271
2.023GlnGln: 2.023 ± 0.465
2.322GlnArg: 2.322 ± 0.44
2.697GlnSer: 2.697 ± 0.56
2.173GlnThr: 2.173 ± 0.411
2.397GlnVal: 2.397 ± 0.366
0.375GlnTrp: 0.375 ± 0.178
1.873GlnTyr: 1.873 ± 0.361
0.0GlnXaa: 0.0 ± 0.0
Arg
2.622ArgAla: 2.622 ± 0.481
0.375ArgCys: 0.375 ± 0.176
2.922ArgAsp: 2.922 ± 0.45
2.397ArgGlu: 2.397 ± 0.417
1.873ArgPhe: 1.873 ± 0.316
2.772ArgGly: 2.772 ± 0.416
1.199ArgHis: 1.199 ± 0.286
3.371ArgIle: 3.371 ± 0.498
3.296ArgLys: 3.296 ± 0.396
4.495ArgLeu: 4.495 ± 0.587
1.648ArgMet: 1.648 ± 0.364
2.472ArgAsn: 2.472 ± 0.517
1.349ArgPro: 1.349 ± 0.318
1.573ArgGln: 1.573 ± 0.367
2.772ArgArg: 2.772 ± 0.525
2.847ArgSer: 2.847 ± 0.466
3.596ArgThr: 3.596 ± 0.56
3.296ArgVal: 3.296 ± 0.539
0.45ArgTrp: 0.45 ± 0.195
1.948ArgTyr: 1.948 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
6.518SerAla: 6.518 ± 0.982
0.45SerCys: 0.45 ± 0.196
4.87SerAsp: 4.87 ± 0.541
4.495SerGlu: 4.495 ± 0.615
2.547SerPhe: 2.547 ± 0.374
5.094SerGly: 5.094 ± 0.663
1.498SerHis: 1.498 ± 0.352
3.221SerIle: 3.221 ± 0.383
5.694SerLys: 5.694 ± 0.761
5.094SerLeu: 5.094 ± 0.688
2.322SerMet: 2.322 ± 0.414
3.821SerAsn: 3.821 ± 0.406
1.948SerPro: 1.948 ± 0.359
2.997SerGln: 2.997 ± 0.406
2.997SerArg: 2.997 ± 0.456
6.068SerSer: 6.068 ± 0.809
4.046SerThr: 4.046 ± 0.561
5.019SerVal: 5.019 ± 0.602
0.899SerTrp: 0.899 ± 0.218
2.397SerTyr: 2.397 ± 0.467
0.0SerXaa: 0.0 ± 0.0
Thr
4.57ThrAla: 4.57 ± 0.836
0.3ThrCys: 0.3 ± 0.131
4.42ThrAsp: 4.42 ± 0.454
2.997ThrGlu: 2.997 ± 0.485
2.098ThrPhe: 2.098 ± 0.41
4.42ThrGly: 4.42 ± 0.646
1.049ThrHis: 1.049 ± 0.285
4.27ThrIle: 4.27 ± 0.519
5.319ThrLys: 5.319 ± 0.71
4.645ThrLeu: 4.645 ± 0.642
1.873ThrMet: 1.873 ± 0.337
2.322ThrAsn: 2.322 ± 0.348
2.098ThrPro: 2.098 ± 0.369
2.622ThrGln: 2.622 ± 0.451
2.772ThrArg: 2.772 ± 0.416
4.345ThrSer: 4.345 ± 0.459
4.12ThrThr: 4.12 ± 0.759
4.195ThrVal: 4.195 ± 0.58
0.824ThrTrp: 0.824 ± 0.211
1.798ThrTyr: 1.798 ± 0.369
0.0ThrXaa: 0.0 ± 0.0
Val
5.244ValAla: 5.244 ± 0.84
0.3ValCys: 0.3 ± 0.143
5.019ValAsp: 5.019 ± 0.564
2.922ValGlu: 2.922 ± 0.469
2.023ValPhe: 2.023 ± 0.356
4.645ValGly: 4.645 ± 0.547
1.049ValHis: 1.049 ± 0.272
5.544ValIle: 5.544 ± 0.626
5.769ValLys: 5.769 ± 0.568
3.221ValLeu: 3.221 ± 0.496
1.498ValMet: 1.498 ± 0.321
3.371ValAsn: 3.371 ± 0.487
2.697ValPro: 2.697 ± 0.373
1.274ValGln: 1.274 ± 0.288
2.997ValArg: 2.997 ± 0.458
4.495ValSer: 4.495 ± 0.629
3.671ValThr: 3.671 ± 0.608
4.72ValVal: 4.72 ± 0.618
0.899ValTrp: 0.899 ± 0.214
1.798ValTyr: 1.798 ± 0.394
0.0ValXaa: 0.0 ± 0.0
Trp
0.824TrpAla: 0.824 ± 0.249
0.15TrpCys: 0.15 ± 0.111
0.599TrpAsp: 0.599 ± 0.232
0.375TrpGlu: 0.375 ± 0.16
0.899TrpPhe: 0.899 ± 0.275
0.599TrpGly: 0.599 ± 0.23
0.45TrpHis: 0.45 ± 0.181
1.199TrpIle: 1.199 ± 0.288
1.049TrpLys: 1.049 ± 0.236
1.723TrpLeu: 1.723 ± 0.423
0.3TrpMet: 0.3 ± 0.162
1.349TrpAsn: 1.349 ± 0.293
0.45TrpPro: 0.45 ± 0.163
0.674TrpGln: 0.674 ± 0.236
0.749TrpArg: 0.749 ± 0.197
1.049TrpSer: 1.049 ± 0.252
0.674TrpThr: 0.674 ± 0.187
1.199TrpVal: 1.199 ± 0.297
0.3TrpTrp: 0.3 ± 0.155
0.3TrpTyr: 0.3 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.997TyrAla: 2.997 ± 0.428
0.225TyrCys: 0.225 ± 0.116
2.622TyrAsp: 2.622 ± 0.449
1.723TyrGlu: 1.723 ± 0.34
1.573TyrPhe: 1.573 ± 0.374
3.072TyrGly: 3.072 ± 0.484
0.45TyrHis: 0.45 ± 0.196
1.798TyrIle: 1.798 ± 0.354
1.723TyrLys: 1.723 ± 0.341
3.147TyrLeu: 3.147 ± 0.577
1.274TyrMet: 1.274 ± 0.32
2.098TyrAsn: 2.098 ± 0.435
0.974TyrPro: 0.974 ± 0.275
1.723TyrGln: 1.723 ± 0.384
2.023TyrArg: 2.023 ± 0.39
2.322TyrSer: 2.322 ± 0.409
2.023TyrThr: 2.023 ± 0.304
2.173TyrVal: 2.173 ± 0.399
0.524TyrTrp: 0.524 ± 0.174
1.349TyrTyr: 1.349 ± 0.438
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.075XaaGly: 0.075 ± 0.07
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (13349 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski