Candidatus Parvarchaeum acidiphilum ARMAN-4

Taxonomy: cellular organisms; Archaea; DPANN group; Candidatus Parvarchaeota; Candidatus Parvarchaeum; Candidatus Parvarchaeum acidiphilum

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2EGA5|D2EGA5_PARA4 Uncharacterized protein OS=Candidatus Parvarchaeum acidiphilum ARMAN-4 OX=662760 GN=BJBARM4_0805 PE=4 SV=1
EEE2 pKa = 5.18INDDD6 pKa = 3.68GGEEE10 pKa = 4.44THHH13 pKa = 5.75TPPNPLVEEE22 pKa = 4.28EE23 pKa = 4.15VIAEEE28 pKa = 4.12YY29 pKa = 10.4WKKK32 pKa = 10.33SEEE35 pKa = 5.15PSDDD39 pKa = 4.92EEE41 pKa = 3.88LSLFGNPSSLTGPPVVTCGGGNLALGDDD69 pKa = 4.25VMLMLEEE76 pKa = 4.24EE77 pKa = 4.68GMNCSGKKK85 pKa = 10.3YY86 pKa = 9.58PAILDDD92 pKa = 3.44VNDDD96 pKa = 4.72KK97 pKa = 10.83ISTVNNLPSITPQNYYY113 pKa = 7.33EEE115 pKa = 4.87LTIVNSTFAEEE126 pKa = 3.94GAFNFSEEE134 pKa = 4.92YYY136 pKa = 9.61

Molecular weight: 14.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2EFS7|D2EFS7_PARA4 Peptidase M50 OS=Candidatus Parvarchaeum acidiphilum ARMAN-4 OX=662760 GN=BJBARM4_0602 PE=4 SV=1
MM1 pKa = 7.9AEE3 pKa = 4.01RR4 pKa = 11.84SNPVADD10 pKa = 3.81NRR12 pKa = 11.84LFYY15 pKa = 10.87NVYY18 pKa = 9.89VCRR21 pKa = 11.84NCNAKK26 pKa = 9.75IRR28 pKa = 11.84VSSQKK33 pKa = 9.03VLRR36 pKa = 11.84RR37 pKa = 11.84NEE39 pKa = 3.86VRR41 pKa = 11.84CRR43 pKa = 11.84NCGSSALRR51 pKa = 11.84PKK53 pKa = 10.49HH54 pKa = 6.6KK55 pKa = 9.98EE56 pKa = 3.58LRR58 pKa = 11.84KK59 pKa = 10.36LKK61 pKa = 10.44VV62 pKa = 2.93

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

911

0

911

236784

31

3049

259.9

29.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.868 ± 0.084

0.699 ± 0.034

4.955 ± 0.082

6.279 ± 0.116

5.113 ± 0.078

6.228 ± 0.075

1.18 ± 0.028

9.417 ± 0.106

8.972 ± 0.166

9.417 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.036

6.413 ± 0.117

3.264 ± 0.057

2.333 ± 0.04

3.311 ± 0.081

8.224 ± 0.163

4.941 ± 0.097

6.157 ± 0.064

0.672 ± 0.025

4.314 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski