Zygosaccharomyces parabailii
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9766 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S7HPB4|A0A1S7HPB4_9SACH Uncharacterized protein OS=Zygosaccharomyces parabailii OX=1365886 GN=BZL39_H02390 PE=4 SV=1
MM1 pKa = 7.53 SLPEE5 pKa = 3.82 VFQFSVHH12 pKa = 5.87 TPPSLLSDD20 pKa = 4.55 SITEE24 pKa = 4.31 PEE26 pKa = 3.76 EE27 pKa = 3.68 STYY30 pKa = 10.42 QEE32 pKa = 4.46 EE33 pKa = 4.56 LLSTPLLIEE42 pKa = 4.01 DD43 pKa = 4.15 TMFKK47 pKa = 10.77 LDD49 pKa = 4.07 LEE51 pKa = 4.54 EE52 pKa = 4.57 PLIEE56 pKa = 4.33 QHH58 pKa = 6.09 DD59 pKa = 4.13 TCLVDD64 pKa = 3.64 WSPQEE69 pKa = 4.69 DD70 pKa = 3.8 MGSEE74 pKa = 4.33 FANAAQTNYY83 pKa = 10.67 RR84 pKa = 11.84 LWLGQAVV91 pKa = 3.37
Molecular weight: 10.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.77
IPC_protein 3.668
Toseland 3.503
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.516
Grimsley 3.414
Solomon 3.605
Lehninger 3.567
Nozaki 3.77
DTASelect 3.872
Thurlkill 3.554
EMBOSS 3.554
Sillero 3.783
Patrickios 1.812
IPC_peptide 3.605
IPC2_peptide 3.757
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A1S7HU20|A0A1S7HU20_9SACH YGL082W and YPL191C OS=Zygosaccharomyces parabailii OX=1365886 GN=ZPAR0K01420_B PE=4 SV=1
MM1 pKa = 7.73 LSLARR6 pKa = 11.84 NAFQASARR14 pKa = 11.84 RR15 pKa = 11.84 TFITIGNFSPLKK27 pKa = 10.09 TMGSLPLQRR36 pKa = 11.84 LLGPQLPFGMGQRR49 pKa = 11.84 RR50 pKa = 11.84 WKK52 pKa = 10.8 SRR54 pKa = 11.84 GNTYY58 pKa = 10.31 QPSTLKK64 pKa = 10.31 RR65 pKa = 11.84 KK66 pKa = 9.59 RR67 pKa = 11.84 RR68 pKa = 11.84 VGFLARR74 pKa = 11.84 ARR76 pKa = 11.84 SKK78 pKa = 10.66 QGSKK82 pKa = 9.72 ILQRR86 pKa = 11.84 RR87 pKa = 11.84 KK88 pKa = 9.93 QKK90 pKa = 10.44 GRR92 pKa = 11.84 WFLTHH97 pKa = 7.07
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.489
IPC2_protein 11.257
IPC_protein 12.661
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.822
Wikipedia 13.29
Rodwell 12.515
Grimsley 12.866
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.822
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.237
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9766
0
9766
4869795
25
4919
498.6
56.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.255 ± 0.022
1.368 ± 0.011
5.647 ± 0.016
6.772 ± 0.023
4.304 ± 0.017
5.586 ± 0.024
2.301 ± 0.01
5.598 ± 0.018
6.642 ± 0.026
9.762 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.008
4.968 ± 0.017
4.594 ± 0.023
4.348 ± 0.024
4.978 ± 0.017
8.699 ± 0.048
5.461 ± 0.031
6.123 ± 0.017
1.114 ± 0.007
3.313 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here