Klebsiella phage K5-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Klebsiella virus K5-2

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A219YH87|A0A219YH87_9CAUD Uncharacterized protein OS=Klebsiella phage K5-2 OX=1932361 GN=k52_006 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.1YY9 pKa = 10.68DD10 pKa = 4.19LLAATVEE17 pKa = 4.19LFNARR22 pKa = 11.84IQQDD26 pKa = 4.22EE27 pKa = 4.44LSMFSDD33 pKa = 3.38WSDD36 pKa = 3.28ALHH39 pKa = 6.1EE40 pKa = 4.49VVDD43 pKa = 4.59GQVPHH48 pKa = 6.81YY49 pKa = 8.66YY50 pKa = 10.32HH51 pKa = 7.62EE52 pKa = 4.81IFTVMAADD60 pKa = 5.45GIDD63 pKa = 4.46LEE65 pKa = 4.93FDD67 pKa = 3.76DD68 pKa = 5.97SGLIPDD74 pKa = 4.27TKK76 pKa = 10.52DD77 pKa = 3.0VSRR80 pKa = 11.84ICQARR85 pKa = 11.84IYY87 pKa = 9.16EE88 pKa = 4.1ALYY91 pKa = 10.87NDD93 pKa = 3.61VSNDD97 pKa = 3.23SGIVWWEE104 pKa = 3.87DD105 pKa = 3.4EE106 pKa = 4.3EE107 pKa = 6.57DD108 pKa = 4.89DD109 pKa = 6.28EE110 pKa = 5.4EE111 pKa = 6.29DD112 pKa = 5.06CDD114 pKa = 4.89DD115 pKa = 3.87

Molecular weight: 13.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219YHB9|A0A219YHB9_9CAUD Uncharacterized protein OS=Klebsiella phage K5-2 OX=1932361 GN=k52_033 PE=4 SV=1
MM1 pKa = 7.4PLWMSLHH8 pKa = 5.29YY9 pKa = 10.53ARR11 pKa = 11.84RR12 pKa = 11.84SYY14 pKa = 11.03VKK16 pKa = 10.32SEE18 pKa = 3.94KK19 pKa = 9.9QRR21 pKa = 11.84EE22 pKa = 4.17ALCLSSQRR30 pKa = 11.84EE31 pKa = 3.93SSRR34 pKa = 11.84ILWLSWCLPSGGTWVHH50 pKa = 6.39NLRR53 pKa = 11.84TPNGRR58 pKa = 11.84RR59 pKa = 11.84KK60 pKa = 10.33YY61 pKa = 10.66RR62 pKa = 11.84MSTLRR67 pKa = 11.84SKK69 pKa = 10.79RR70 pKa = 11.84LEE72 pKa = 3.91LKK74 pKa = 9.87LRR76 pKa = 11.84KK77 pKa = 9.48RR78 pKa = 11.84LTQYY82 pKa = 11.07RR83 pKa = 11.84LSTKK87 pKa = 8.29QTLRR91 pKa = 11.84GWRR94 pKa = 11.84AALIGLLLICVATISGCASEE114 pKa = 5.38SNLPVSPQDD123 pKa = 3.42QTVDD127 pKa = 3.23ASLMVPSNYY136 pKa = 9.09TKK138 pKa = 10.58QLLEE142 pKa = 4.16VLSQQ146 pKa = 3.24

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

12413

67

1321

295.5

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.111 ± 0.45

1.08 ± 0.168

6.332 ± 0.244

6.727 ± 0.431

3.504 ± 0.155

8.241 ± 0.52

1.861 ± 0.188

4.761 ± 0.196

6.38 ± 0.445

8.225 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.198

4.213 ± 0.23

3.69 ± 0.178

4.229 ± 0.284

5.446 ± 0.248

6.284 ± 0.357

5.671 ± 0.326

6.759 ± 0.369

1.426 ± 0.193

3.504 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski