Alloprevotella sp. E39
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2261 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8E7P5|A0A5P8E7P5_9BACT SAM-dependent methyltransferase OS=Alloprevotella sp. E39 OX=2133944 GN=C7Y71_008150 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.34 KK3 pKa = 9.02 TIVVYY8 pKa = 10.71 GSTTGSCQDD17 pKa = 3.44 FAEE20 pKa = 5.23 KK21 pKa = 10.08 IASKK25 pKa = 10.55 LGEE28 pKa = 4.16 SDD30 pKa = 3.63 VMNVSDD36 pKa = 4.89 VSADD40 pKa = 3.42 TLAEE44 pKa = 3.97 YY45 pKa = 11.04 DD46 pKa = 3.75 NLLLGSSTWGAGEE59 pKa = 5.13 LQDD62 pKa = 3.97 DD63 pKa = 4.49 WYY65 pKa = 10.87 DD66 pKa = 3.52 GVEE69 pKa = 4.14 TLKK72 pKa = 10.6 ATDD75 pKa = 3.95 LSGKK79 pKa = 7.51 TVAIFGCGDD88 pKa = 3.25 AEE90 pKa = 4.86 GYY92 pKa = 11.4 GDD94 pKa = 4.05 TFCGAMAEE102 pKa = 4.42 IYY104 pKa = 10.63 NAVKK108 pKa = 10.64 DD109 pKa = 3.73 SGANIIGQVPADD121 pKa = 3.71 TYY123 pKa = 11.23 SYY125 pKa = 10.2 EE126 pKa = 4.2 DD127 pKa = 3.65 SEE129 pKa = 4.48 AVSDD133 pKa = 4.05 GMFVGLALDD142 pKa = 3.98 CTEE145 pKa = 4.38 SDD147 pKa = 3.53 NNSEE151 pKa = 3.9 EE152 pKa = 6.1 RR153 pKa = 11.84 IDD155 pKa = 3.42 NWLNNIRR162 pKa = 11.84 PEE164 pKa = 4.03 LL165 pKa = 3.89
Molecular weight: 17.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.808
Patrickios 0.985
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A5P8E4W5|A0A5P8E4W5_9BACT VOC family protein OS=Alloprevotella sp. E39 OX=2133944 GN=C7Y71_002535 PE=4 SV=1
MM1 pKa = 7.61 FFPIFPVLSLSVIGFIGYY19 pKa = 9.41 NGGMEE24 pKa = 3.95 PCFRR28 pKa = 11.84 MKK30 pKa = 9.87 MQIRR34 pKa = 11.84 MKK36 pKa = 10.02 ILKK39 pKa = 9.25 CRR41 pKa = 11.84 LVIPLSPPPRR51 pKa = 11.84 CGPGVASPIAQLVRR65 pKa = 11.84 APHH68 pKa = 5.78
Molecular weight: 7.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.604
IPC_protein 10.189
Toseland 10.672
ProMoST 10.292
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 11.052
Grimsley 10.789
Solomon 10.862
Lehninger 10.847
Nozaki 10.701
DTASelect 10.423
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.701
Patrickios 10.906
IPC_peptide 10.877
IPC2_peptide 9.838
IPC2.peptide.svr19 8.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2261
0
2261
846569
28
3648
374.4
42.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.491 ± 0.054
1.324 ± 0.021
5.813 ± 0.031
6.428 ± 0.054
4.352 ± 0.036
6.953 ± 0.05
1.948 ± 0.022
6.614 ± 0.047
6.315 ± 0.05
8.589 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.023
5.314 ± 0.047
3.696 ± 0.029
3.423 ± 0.028
4.809 ± 0.039
6.216 ± 0.05
6.059 ± 0.062
6.493 ± 0.037
1.212 ± 0.02
4.314 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here