Alloprevotella sp. E39

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Alloprevotella; unclassified Alloprevotella

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5P8E7P5|A0A5P8E7P5_9BACT SAM-dependent methyltransferase OS=Alloprevotella sp. E39 OX=2133944 GN=C7Y71_008150 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 9.34KK3 pKa = 9.02TIVVYY8 pKa = 10.71GSTTGSCQDD17 pKa = 3.44FAEE20 pKa = 5.23KK21 pKa = 10.08IASKK25 pKa = 10.55LGEE28 pKa = 4.16SDD30 pKa = 3.63VMNVSDD36 pKa = 4.89VSADD40 pKa = 3.42TLAEE44 pKa = 3.97YY45 pKa = 11.04DD46 pKa = 3.75NLLLGSSTWGAGEE59 pKa = 5.13LQDD62 pKa = 3.97DD63 pKa = 4.49WYY65 pKa = 10.87DD66 pKa = 3.52GVEE69 pKa = 4.14TLKK72 pKa = 10.6ATDD75 pKa = 3.95LSGKK79 pKa = 7.51TVAIFGCGDD88 pKa = 3.25AEE90 pKa = 4.86GYY92 pKa = 11.4GDD94 pKa = 4.05TFCGAMAEE102 pKa = 4.42IYY104 pKa = 10.63NAVKK108 pKa = 10.64DD109 pKa = 3.73SGANIIGQVPADD121 pKa = 3.71TYY123 pKa = 11.23SYY125 pKa = 10.2EE126 pKa = 4.2DD127 pKa = 3.65SEE129 pKa = 4.48AVSDD133 pKa = 4.05GMFVGLALDD142 pKa = 3.98CTEE145 pKa = 4.38SDD147 pKa = 3.53NNSEE151 pKa = 3.9EE152 pKa = 6.1RR153 pKa = 11.84IDD155 pKa = 3.42NWLNNIRR162 pKa = 11.84PEE164 pKa = 4.03LL165 pKa = 3.89

Molecular weight:
17.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5P8E4W5|A0A5P8E4W5_9BACT VOC family protein OS=Alloprevotella sp. E39 OX=2133944 GN=C7Y71_002535 PE=4 SV=1
MM1 pKa = 7.61FFPIFPVLSLSVIGFIGYY19 pKa = 9.41NGGMEE24 pKa = 3.95PCFRR28 pKa = 11.84MKK30 pKa = 9.87MQIRR34 pKa = 11.84MKK36 pKa = 10.02ILKK39 pKa = 9.25CRR41 pKa = 11.84LVIPLSPPPRR51 pKa = 11.84CGPGVASPIAQLVRR65 pKa = 11.84APHH68 pKa = 5.78

Molecular weight:
7.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2261

0

2261

846569

28

3648

374.4

42.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.491 ± 0.054

1.324 ± 0.021

5.813 ± 0.031

6.428 ± 0.054

4.352 ± 0.036

6.953 ± 0.05

1.948 ± 0.022

6.614 ± 0.047

6.315 ± 0.05

8.589 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.023

5.314 ± 0.047

3.696 ± 0.029

3.423 ± 0.028

4.809 ± 0.039

6.216 ± 0.05

6.059 ± 0.062

6.493 ± 0.037

1.212 ± 0.02

4.314 ± 0.039

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski