Permianibacter aggregans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Permianibacter

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6UMW3|A0A4R6UMW3_9GAMM Uncharacterized protein OS=Permianibacter aggregans OX=1510150 GN=EV696_11346 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 9.5MKK4 pKa = 10.26KK5 pKa = 10.07VFGAAVLAIASMVSHH20 pKa = 6.68SAFADD25 pKa = 4.76LILPTDD31 pKa = 3.91PLVKK35 pKa = 9.89TNHH38 pKa = 6.68PSLNTGNVGGDD49 pKa = 3.66PVSIAATSGCASSDD63 pKa = 3.65YY64 pKa = 10.26CTLTEE69 pKa = 5.13LINGGSLSAGGLTFGNFNLDD89 pKa = 2.88FDD91 pKa = 4.85FGYY94 pKa = 11.3GDD96 pKa = 5.65ALDD99 pKa = 4.33TDD101 pKa = 4.53NVWVRR106 pKa = 11.84VFDD109 pKa = 3.92FGGAIMLDD117 pKa = 3.66YY118 pKa = 11.28DD119 pKa = 4.33FAPLGFGGPLPTLLSGEE136 pKa = 3.88ASGFIDD142 pKa = 4.01LSYY145 pKa = 11.32SVVSDD150 pKa = 3.35SSVDD154 pKa = 4.16IFAAWLWDD162 pKa = 3.66MIGAGTADD170 pKa = 4.3ADD172 pKa = 3.98YY173 pKa = 10.97EE174 pKa = 4.35LQFDD178 pKa = 3.73MTLFDD183 pKa = 5.37GDD185 pKa = 3.81TEE187 pKa = 4.19LAYY190 pKa = 10.72LYY192 pKa = 10.45LYY194 pKa = 10.89SLLEE198 pKa = 4.25NNSVIDD204 pKa = 4.25SDD206 pKa = 5.07ADD208 pKa = 3.59DD209 pKa = 4.4LAIFAALNSLNVWNSISAISYY230 pKa = 8.47EE231 pKa = 4.02GLNLFYY237 pKa = 10.51IGDD240 pKa = 3.46QMFFTEE246 pKa = 4.75VTQVPAPFTVLLFGFGLAALGLRR269 pKa = 11.84KK270 pKa = 9.3RR271 pKa = 11.84VRR273 pKa = 3.23

Molecular weight: 29.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6UD45|A0A4R6UD45_9GAMM Citrate lyase subunit beta/citryl-CoA lyase OS=Permianibacter aggregans OX=1510150 GN=EV696_13315 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.17KK12 pKa = 10.64KK13 pKa = 9.6RR14 pKa = 11.84DD15 pKa = 3.36HH16 pKa = 6.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39NGRR28 pKa = 11.84LVLKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.6GRR39 pKa = 11.84KK40 pKa = 8.83RR41 pKa = 11.84LTVVV45 pKa = 3.08

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3735

0

3735

1253671

31

13667

335.7

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.264 ± 0.046

0.932 ± 0.016

5.347 ± 0.029

6.078 ± 0.042

3.937 ± 0.029

7.014 ± 0.047

2.404 ± 0.02

5.262 ± 0.026

4.055 ± 0.04

10.943 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.025

3.489 ± 0.035

4.562 ± 0.024

4.92 ± 0.038

6.177 ± 0.035

5.995 ± 0.042

4.995 ± 0.033

6.984 ± 0.037

1.528 ± 0.021

2.684 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski