Tortoise microvirus 41
Average proteome isoelectric point is 5.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVY3|A0A4V1FVY3_9VIRU Major capsid protein OS=Tortoise microvirus 41 OX=2583145 PE=3 SV=1
MM1 pKa = 7.76 SDD3 pKa = 4.67 FIPIDD8 pKa = 3.67 FFTDD12 pKa = 3.02 DD13 pKa = 4.25 GYY15 pKa = 11.87 APVPVRR21 pKa = 11.84 YY22 pKa = 8.71 LASPEE27 pKa = 3.86 TAEE30 pKa = 3.88 VFNRR34 pKa = 11.84 VMSMAPTFFPDD45 pKa = 4.22 LPVDD49 pKa = 4.28 ANPVIAEE56 pKa = 3.94 QQYY59 pKa = 11.45 AMALVTLAVNFYY71 pKa = 10.79 FDD73 pKa = 3.9 TLGLLSPPEE82 pKa = 4.25 VAPSTPVGDD91 pKa = 3.93 DD92 pKa = 3.19 VLDD95 pKa = 3.77 IKK97 pKa = 10.49 EE98 pKa = 4.2 SRR100 pKa = 11.84 VV101 pKa = 3.25
Molecular weight: 11.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.081
IPC2_protein 3.656
IPC_protein 3.605
Toseland 3.389
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.757
DTASelect 3.973
Thurlkill 3.478
EMBOSS 3.592
Sillero 3.732
Patrickios 3.02
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A4P8W6H2|A0A4P8W6H2_9VIRU Uncharacterized protein OS=Tortoise microvirus 41 OX=2583145 PE=4 SV=1
MM1 pKa = 7.48 SCLSPVTIHH10 pKa = 6.62 VNKK13 pKa = 10.03 QNYY16 pKa = 7.41 EE17 pKa = 4.28 VPCRR21 pKa = 11.84 QCLSCRR27 pKa = 11.84 LDD29 pKa = 3.28 YY30 pKa = 11.34 QMYY33 pKa = 8.78 LTFCVEE39 pKa = 3.77 NEE41 pKa = 4.22 LYY43 pKa = 10.68 DD44 pKa = 3.74 LYY46 pKa = 11.42 KK47 pKa = 10.53 RR48 pKa = 11.84 GLGATFCTFTYY59 pKa = 10.8 SDD61 pKa = 4.46 ANLPPNGSLRR71 pKa = 11.84 KK72 pKa = 9.41 SDD74 pKa = 3.74 LQNLFKK80 pKa = 10.29 TVRR83 pKa = 11.84 TTAKK87 pKa = 9.88 RR88 pKa = 11.84 DD89 pKa = 3.2 GFLPSFKK96 pKa = 10.75 YY97 pKa = 9.81 IACGEE102 pKa = 4.18 YY103 pKa = 10.04 GDD105 pKa = 4.41 KK106 pKa = 10.53 FGRR109 pKa = 11.84 PHH111 pKa = 5.0 YY112 pKa = 10.21 HH113 pKa = 6.23 AVILGWSDD121 pKa = 3.85 VICHH125 pKa = 5.67 NMFRR129 pKa = 11.84 KK130 pKa = 9.15 YY131 pKa = 6.7 WTYY134 pKa = 10.59 GLSDD138 pKa = 4.11 FGVLRR143 pKa = 11.84 PGGVRR148 pKa = 11.84 YY149 pKa = 9.48 VLKK152 pKa = 10.73 YY153 pKa = 7.99 CTKK156 pKa = 10.14 SVRR159 pKa = 11.84 GTKK162 pKa = 10.19 AEE164 pKa = 3.98 EE165 pKa = 4.25 MYY167 pKa = 10.64 DD168 pKa = 3.53 AQGLEE173 pKa = 4.24 RR174 pKa = 11.84 PFISHH179 pKa = 5.12 SHH181 pKa = 6.35 KK182 pKa = 10.38 IGYY185 pKa = 8.86 DD186 pKa = 3.31 WLCRR190 pKa = 11.84 NADD193 pKa = 4.24 DD194 pKa = 4.84 IVANNFTYY202 pKa = 10.82 LNNGVRR208 pKa = 11.84 RR209 pKa = 11.84 PIPKK213 pKa = 9.86 YY214 pKa = 10.43 FRR216 pKa = 11.84 DD217 pKa = 3.46 KK218 pKa = 11.04 YY219 pKa = 11.44 DD220 pKa = 3.2 YY221 pKa = 10.93 FKK223 pKa = 10.77 TFNPMPAVEE232 pKa = 4.01 FLKK235 pKa = 10.9 RR236 pKa = 11.84 EE237 pKa = 4.41 GALHH241 pKa = 7.05 SYY243 pKa = 10.02 PDD245 pKa = 3.37 YY246 pKa = 11.28 RR247 pKa = 11.84 EE248 pKa = 3.76 WSKK251 pKa = 11.37 VKK253 pKa = 10.42 AYY255 pKa = 10.57 NKK257 pKa = 10.13 EE258 pKa = 3.58 KK259 pKa = 11.01 GLIQRR264 pKa = 11.84 TRR266 pKa = 11.84 QEE268 pKa = 4.91 GIPVDD273 pKa = 4.35 DD274 pKa = 5.09 GNFIATPLPSPSLAPSLVTEE294 pKa = 4.22 VLNRR298 pKa = 11.84 PP299 pKa = 3.68
Molecular weight: 34.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.404
IPC2_protein 8.361
IPC_protein 8.229
Toseland 8.302
ProMoST 8.712
Dawson 8.946
Bjellqvist 9.077
Wikipedia 9.033
Rodwell 9.004
Grimsley 8.434
Solomon 9.033
Lehninger 9.004
Nozaki 9.151
DTASelect 8.887
Thurlkill 8.931
EMBOSS 9.092
Sillero 9.18
Patrickios 3.884
IPC_peptide 9.019
IPC2_peptide 8.039
IPC2.peptide.svr19 7.965
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1846
83
579
230.8
25.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.692 ± 1.218
1.408 ± 0.571
6.23 ± 0.474
4.605 ± 0.646
4.605 ± 0.542
5.905 ± 0.58
1.679 ± 0.196
4.55 ± 0.714
3.684 ± 0.936
9.263 ± 1.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.492 ± 0.231
5.038 ± 0.398
6.013 ± 0.734
4.713 ± 1.802
5.525 ± 0.359
8.613 ± 0.978
6.23 ± 0.548
5.905 ± 0.576
0.65 ± 0.147
5.2 ± 0.865
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here