Flavihumibacter soli
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3820 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B3PFP5|A0A6B3PFP5_9BACT FadR family transcriptional regulator OS=Flavihumibacter soli OX=2705549 GN=GZH53_01795 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.21 SQYY5 pKa = 9.86 PLLSASCLYY14 pKa = 10.22 TEE16 pKa = 4.63 PLDD19 pKa = 3.85 YY20 pKa = 11.38 LEE22 pKa = 4.66 NFCGIRR28 pKa = 11.84 QVTKK32 pKa = 10.76 EE33 pKa = 3.68 FVQFLKK39 pKa = 10.89 SEE41 pKa = 4.06 SGKK44 pKa = 10.56 IMEE47 pKa = 5.18 EE48 pKa = 4.26 DD49 pKa = 3.62 SDD51 pKa = 5.19 DD52 pKa = 4.43 AGQALNTEE60 pKa = 4.59 EE61 pKa = 4.7 GDD63 pKa = 3.64 NPASFFYY70 pKa = 9.88 TQTFTPSSEE79 pKa = 4.19 DD80 pKa = 3.12
Molecular weight: 9.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.999
IPC_protein 3.884
Toseland 3.706
ProMoST 3.935
Dawson 3.846
Bjellqvist 4.075
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.77
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.929
Protein with the highest isoelectric point:
>tr|A0A6B3PW20|A0A6B3PW20_9BACT Uncharacterized protein OS=Flavihumibacter soli OX=2705549 GN=GZH53_14950 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.54 RR3 pKa = 11.84 TFQPSQRR10 pKa = 11.84 KK11 pKa = 8.72 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.3 HH16 pKa = 3.93 GFRR19 pKa = 11.84 EE20 pKa = 4.26 RR21 pKa = 11.84 MSSANGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.31 GRR39 pKa = 11.84 KK40 pKa = 8.36 RR41 pKa = 11.84 VSVSDD46 pKa = 3.68 VRR48 pKa = 11.84 RR49 pKa = 11.84 HH50 pKa = 5.0 KK51 pKa = 10.94 AA52 pKa = 2.9
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.383
IPC2_protein 10.818
IPC_protein 12.398
Toseland 12.574
ProMoST 13.071
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.042
Rodwell 12.252
Grimsley 12.618
Solomon 13.071
Lehninger 12.969
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 11.974
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3820
0
3820
1307137
38
3478
342.2
38.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.281 ± 0.04
0.752 ± 0.012
5.393 ± 0.026
6.049 ± 0.04
4.964 ± 0.031
6.961 ± 0.034
1.819 ± 0.019
7.288 ± 0.037
6.616 ± 0.039
9.602 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.251 ± 0.021
5.39 ± 0.04
3.916 ± 0.023
3.527 ± 0.02
4.365 ± 0.028
6.867 ± 0.038
5.553 ± 0.042
6.387 ± 0.03
1.146 ± 0.015
3.872 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here