Mycoplasma sp. OR1901
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 651 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6N1DVW2|A0A6N1DVW2_9MOLU 50S ribosomal protein L33 OS=Mycoplasma sp. OR1901 OX=2742195 GN=rpmG PE=3 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.4 LNKK5 pKa = 9.59 KK6 pKa = 7.61 VLKK9 pKa = 10.59 NILITLSGISVVTTVSCSQSQAKK32 pKa = 9.76 QEE34 pKa = 4.19 VKK36 pKa = 10.73 KK37 pKa = 10.61 EE38 pKa = 4.19 DD39 pKa = 3.63 NSGTNADD46 pKa = 3.81 TNVVKK51 pKa = 10.83 EE52 pKa = 4.2 NDD54 pKa = 3.7 STSDD58 pKa = 3.28 NVNNNSSNEE67 pKa = 3.82 EE68 pKa = 4.06 STTDD72 pKa = 3.22 STNDD76 pKa = 2.94 SKK78 pKa = 10.28 TKK80 pKa = 10.35 VEE82 pKa = 4.18 EE83 pKa = 4.43 EE84 pKa = 4.42 EE85 pKa = 4.28 VTTPADD91 pKa = 3.51 STNEE95 pKa = 3.76 RR96 pKa = 11.84 TTEE99 pKa = 4.1 GTNSDD104 pKa = 3.25 SSSRR108 pKa = 11.84 EE109 pKa = 4.05 GEE111 pKa = 3.94 NTTTDD116 pKa = 3.14 EE117 pKa = 4.34 KK118 pKa = 10.52 QTEE121 pKa = 4.39 EE122 pKa = 3.97 VDD124 pKa = 4.22 SNSSTGEE131 pKa = 3.93 TSSDD135 pKa = 3.24 GSGSTSGDD143 pKa = 3.11 NSSTGEE149 pKa = 3.89 ASSSEE154 pKa = 3.88 GDD156 pKa = 3.68 STSEE160 pKa = 3.99 GSKK163 pKa = 10.27 SDD165 pKa = 3.34 SSEE168 pKa = 4.28 GEE170 pKa = 4.19 TSSSEE175 pKa = 4.41 SEE177 pKa = 4.11 STPDD181 pKa = 4.46 GEE183 pKa = 4.63 KK184 pKa = 9.13 QTEE187 pKa = 4.44 GTGSSNGEE195 pKa = 4.12 TPSSGEE201 pKa = 3.87 GTTTGEE207 pKa = 4.23 KK208 pKa = 8.39 QTGGADD214 pKa = 3.42 SDD216 pKa = 4.14 SSGEE220 pKa = 4.03 VTSPDD225 pKa = 3.35 EE226 pKa = 4.65 GEE228 pKa = 4.14 SGGDD232 pKa = 3.48 GEE234 pKa = 4.87 TEE236 pKa = 3.93 GTIGSNTDD244 pKa = 3.23 DD245 pKa = 4.5 SGKK248 pKa = 10.41 EE249 pKa = 3.8 NQKK252 pKa = 9.08 QQVKK256 pKa = 9.09 VFVVNPKK263 pKa = 10.08 LNKK266 pKa = 9.77 EE267 pKa = 3.94 KK268 pKa = 9.99 MIEE271 pKa = 4.18 IINSKK276 pKa = 10.07 LKK278 pKa = 10.09 NGQVKK283 pKa = 10.29 LYY285 pKa = 10.24 KK286 pKa = 10.11 YY287 pKa = 11.08 ANTEE291 pKa = 3.63 YY292 pKa = 10.46 FGVYY296 pKa = 9.69 RR297 pKa = 11.84 GGSNEE302 pKa = 3.58 EE303 pKa = 3.48 NRR305 pKa = 11.84 FFKK308 pKa = 10.81 FDD310 pKa = 3.6 VNYY313 pKa = 10.84 SEE315 pKa = 5.93 SNVKK319 pKa = 10.22 LDD321 pKa = 3.54 YY322 pKa = 10.93 PEE324 pKa = 3.99 NAKK327 pKa = 9.3 TKK329 pKa = 9.72 TFLGNWDD336 pKa = 3.56 ADD338 pKa = 3.87 KK339 pKa = 11.19 KK340 pKa = 11.2 VLTISYY346 pKa = 9.26 KK347 pKa = 10.36 YY348 pKa = 10.2 KK349 pKa = 10.74 EE350 pKa = 4.1 EE351 pKa = 4.2 TIEE354 pKa = 4.44 QSFNLSNDD362 pKa = 2.86 EE363 pKa = 4.18 STTDD367 pKa = 3.44 EE368 pKa = 4.65 NGSEE372 pKa = 4.39 DD373 pKa = 3.72 SSSSSSSSSSSSEE386 pKa = 3.72 VSNNN390 pKa = 2.88
Molecular weight: 41.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.437
IPC2_protein 4.202
IPC_protein 4.164
Toseland 3.986
ProMoST 4.253
Dawson 4.101
Bjellqvist 4.253
Wikipedia 3.961
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.05
Nozaki 4.202
DTASelect 4.342
Thurlkill 3.986
EMBOSS 3.973
Sillero 4.266
Patrickios 3.948
IPC_peptide 4.101
IPC2_peptide 4.253
IPC2.peptide.svr19 4.196
Protein with the highest isoelectric point:
>tr|A0A6N1DSX4|A0A6N1DSX4_9MOLU 2 3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Mycoplasma sp. OR1901 OX=2742195 GN=gpmI PE=3 SV=1
MM1 pKa = 7.94 AYY3 pKa = 10.34 NKK5 pKa = 9.78 RR6 pKa = 11.84 KK7 pKa = 9.92 KK8 pKa = 10.1 VFSGRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 VCSFCEE21 pKa = 4.22 SKK23 pKa = 10.54 SQYY26 pKa = 10.55 VDD28 pKa = 3.44 YY29 pKa = 11.33 KK30 pKa = 11.13 DD31 pKa = 5.0 VEE33 pKa = 4.36 LLNNFISATGQIKK46 pKa = 10.25 ARR48 pKa = 11.84 SITGTCAKK56 pKa = 9.88 HH57 pKa = 4.71 QRR59 pKa = 11.84 KK60 pKa = 8.97 VSNAIKK66 pKa = 9.97 RR67 pKa = 11.84 ARR69 pKa = 11.84 FIAFMPYY76 pKa = 8.59 TVVRR80 pKa = 11.84 VRR82 pKa = 11.84 NLSKK86 pKa = 11.05
Molecular weight: 9.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.955
IPC_protein 10.687
Toseland 10.906
ProMoST 10.613
Dawson 10.994
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 11.345
Grimsley 11.038
Solomon 11.111
Lehninger 11.082
Nozaki 10.891
DTASelect 10.672
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.921
Patrickios 11.082
IPC_peptide 11.111
IPC2_peptide 9.721
IPC2.peptide.svr19 8.318
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
651
0
651
288149
37
4018
442.6
50.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.728 ± 0.13
0.254 ± 0.017
6.074 ± 0.075
7.67 ± 0.145
5.011 ± 0.122
4.455 ± 0.123
1.29 ± 0.035
9.032 ± 0.105
10.534 ± 0.103
9.178 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.542 ± 0.051
8.968 ± 0.146
2.435 ± 0.069
3.22 ± 0.078
2.892 ± 0.054
6.771 ± 0.081
5.453 ± 0.063
5.516 ± 0.073
0.845 ± 0.029
4.132 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here